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Cytochrome P450 3A4



Homo sapiens (Human)
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli


Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2-exo-monooxygenase. The enzyme also hydroxylates etoposide (PubMed:11159812). Catalyzes 4-beta-hydroxylation of cholesterol. May catalyze 25-hydroxylation of cholesterol in vitro (PubMed:21576599).2 Publications


Chimeric transcripts, characterized by CYP3A43 exon 1 joined at canonical splice sites to distinct sets of CYP3A4 exons, have been detected. All are possibly produced by trans-splicing. The chimeric transcripts exist in 3 different combinations: CYP3A43 exon 1 joined in frame to CYP3A4 exons 2-13, CYP3A43 exon 1 joined in frame to CYP3A4 exons 4-13 and CYP3A43 exon 1 joined in frame to CYP3A4 exon 7-13. The longest chimeric isoform (CYP3A43 exon 1 joined to CYP3A4 exons 2-13) exhibits 6-beta-hydroxylase activity, while a shorter isoform (CYP3A43 exon 1 joined to CYP3A4 exons 4-13) does not. All chimeric transcripts are expressed at very low levels in the liver (PubMed:11726664).1 Publication

Catalytic activityi

Quinine + NADPH + O2 = 3-hydroxyquinine + NADP+ + H2O.1 Publication
Taurochenodeoxycholate + NADPH + O2 = taurohyocholate + NADP+ + H2O.1 Publication
Lithocholate + NADPH + O2 = hyodeoxycholate + NADP+ + H2O.1 Publication
Albendazole + NADPH + O2 = albendazole S-oxide + NADP+ + H2O.1 Publication
1,8-cineole + NADPH + O2 = 2-exo-hydroxy-1,8-cineole + NADP+ + H2O.1 Publication
RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.1 Publication



Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi442Iron (heme axial ligand)1

GO - Molecular functioni

  • 1,8-cineole 2-exo-monooxygenase activity Source: UniProtKB-EC
  • albendazole monooxygenase activity Source: UniProtKB-EC
  • aromatase activity Source: UniProtKB-EC
  • caffeine oxidase activity Source: BHF-UCL
  • enzyme binding Source: BHF-UCL
  • estrogen 16-alpha-hydroxylase activity Source: BHF-UCL
  • heme binding Source: InterPro
  • iron ion binding Source: BHF-UCL
  • monooxygenase activity Source: BHF-UCL
  • oxidoreductase activity Source: BHF-UCL
  • oxygen binding Source: Reactome
  • quinine 3-monooxygenase activity Source: UniProtKB-EC
  • steroid binding Source: BHF-UCL
  • steroid hydroxylase activity Source: BHF-UCL
  • taurochenodeoxycholate 6alpha-hydroxylase activity Source: UniProtKB-EC
  • testosterone 6-beta-hydroxylase activity Source: BHF-UCL
  • vitamin D 24-hydroxylase activity Source: BHF-UCL
  • vitamin D3 25-hydroxylase activity Source: BHF-UCL

GO - Biological processi

  • alkaloid catabolic process Source: BHF-UCL
  • androgen metabolic process Source: BHF-UCL
  • drug catabolic process Source: BHF-UCL
  • drug metabolic process Source: BHF-UCL
  • exogenous drug catabolic process Source: BHF-UCL
  • heterocycle metabolic process Source: BHF-UCL
  • lipid hydroxylation Source: BHF-UCL
  • lipid metabolic process Source: ProtInc
  • monoterpenoid metabolic process Source: BHF-UCL
  • oxidation-reduction process Source: BHF-UCL
  • oxidative demethylation Source: BHF-UCL
  • steroid catabolic process Source: BHF-UCL
  • steroid metabolic process Source: BHF-UCL
  • vitamin D metabolic process Source: BHF-UCL
  • xenobiotic metabolic process Source: Reactome


Molecular functionMonooxygenase, Oxidoreductase
Biological processLipid metabolism, Steroid metabolism, Sterol metabolism
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.14.13.67. 2681. 2681.
ReactomeiR-HSA-211981. Xenobiotics.
R-HSA-5423646. Aflatoxin activation and detoxification.

Chemistry databases


Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 3A4 (EC:1.14.13.-)
Alternative name(s):
1,8-cineole 2-exo-monooxygenase (EC:
Albendazole monooxygenase (EC:
Albendazole sulfoxidase
Cholesterol 25-hydroxylase1 Publication (EC: Publication)
Cytochrome P450 3A3
Cytochrome P450 HLp
Cytochrome P450 NF-25
Cytochrome P450-PCN1
Nifedipine oxidase
Quinine 3-monooxygenase (EC:
Taurochenodeoxycholate 6-alpha-hydroxylase (EC:
Gene namesi
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases


Subcellular locationi


Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei2 – 22HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • endoplasmic reticulum membrane Source: Reactome
  • integral component of membrane Source: UniProtKB-KW
  • intracellular membrane-bounded organelle Source: ProtInc
  • organelle membrane Source: UniProtKB-SubCell

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Organism-specific databases


Chemistry databases

DrugBankiDB05812. Abiraterone.
DB01418. Acenocoumarol.
DB00316. Acetaminophen.
DB00819. Acetazolamide.
DB00546. Adinazolam.
DB00518. Albendazole.
DB00240. Alclometasone.
DB00041. Aldesleukin.
DB00802. Alfentanil.
DB00346. Alfuzosin.
DB01246. Alimemazine.
DB09026. Aliskiren.
DB01431. Allylestrenol.
DB00918. Almotriptan.
DB06203. Alogliptin.
DB00969. Alosetron.
DB00404. Alprazolam.
DB06403. Ambrisentan.
DB06742. Ambroxol.
DB00357. Aminoglutethimide.
DB01424. Aminophenazone.
DB01223. Aminophylline.
DB01118. Amiodarone.
DB00321. Amitriptyline.
DB00381. Amlodipine.
DB00701. Amprenavir.
DB01217. Anastrozole.
DB01435. Antipyrine.
DB06605. Apixaban.
DB05676. Apremilast.
DB00673. Aprepitant.
DB00278. Argatroban.
DB01238. Aripiprazole.
DB06413. Armodafinil.
DB01169. Arsenic trioxide.
DB06697. Artemether.
DB06216. Asenapine.
DB00637. Astemizole.
DB01072. Atazanavir.
DB00289. Atomoxetine.
DB01076. Atorvastatin.
DB06237. Avanafil.
DB06626. Axitinib.
DB00719. Azatadine.
DB00972. Azelastine.
DB04957. Azimilide.
DB00207. Azithromycin.
DB04975. Banoxantrone.
DB08903. Bedaquiline.
DB00865. Benzphetamine.
DB06770. Benzyl alcohol.
DB00443. Betamethasone.
DB00307. Bexarotene.
DB01393. Bezafibrate.
DB01128. Bicalutamide.
DB04794. Bifonazole.
DB00612. Bisoprolol.
DB08873. Boceprevir.
DB00188. Bortezomib.
DB00559. Bosentan.
DB06616. Bosutinib.
DB08870. Brentuximab vedotin.
DB09128. Brexpiprazole.
DB01194. Brinzolamide.
DB01558. Bromazepam.
DB01200. Bromocriptine.
DB00835. Brompheniramine.
DB01222. Budesonide.
DB00297. Bupivacaine.
DB00921. Buprenorphine.
DB01156. Bupropion.
DB00490. Buspirone.
DB01008. Busulfan.
DB06772. Cabazitaxel.
DB00248. Cabergoline.
DB08875. Cabozantinib.
DB00201. Caffeine.
DB04886. Calanolide A.
DB00136. Calcitriol.
DB08907. Canagliflozin.
DB06774. Capsaicin.
DB00564. Carbamazepine.
DB00748. Carbinoxamine.
DB06016. Cariprazine.
DB01136. Carvedilol.
DB00520. Caspofungin.
DB01333. Cefradine.
DB00482. Celecoxib.
DB04846. Celiprolol.
DB00567. Cephalexin.
DB09063. Ceritinib.
DB00439. Cerivastatin.
DB00185. Cevimeline.
DB06777. Chenodeoxycholic acid.
DB00446. Chloramphenicol.
DB00475. Chlordiazepoxide.
DB00608. Chloroquine.
DB01114. Chlorphenamine.
DB00856. Chlorphenesin.
DB00477. Chlorpromazine.
DB00356. Chlorzoxazone.
DB00169. Cholecalciferol.
DB01410. Ciclesonide.
DB01166. Cilostazol.
DB00501. Cimetidine.
DB01012. Cinacalcet.
DB00537. Ciprofloxacin.
DB00604. Cisapride.
DB00215. Citalopram.
DB01211. Clarithromycin.
DB00283. Clemastine.
DB04920. Clevidipine.
DB01190. Clindamycin.
DB00349. Clobazam.
DB00845. Clofazimine.
DB00636. Clofibrate.
DB00882. Clomifene.
DB01242. Clomipramine.
DB01068. Clonazepam.
DB00575. Clonidine.
DB00758. Clopidogrel.
DB00628. Clorazepate.
DB01559. Clotiazepam.
DB00257. Clotrimazole.
DB00363. Clozapine.
DB09065. Cobicistat.
DB05239. Cobimetinib.
DB00907. Cocaine.
DB00318. Codeine.
DB01394. Colchicine.
DB00872. Conivaptan.
DB00286. Conjugated Equine Estrogens.
DB01380. Cortisone acetate.
DB08865. Crizotinib.
DB00924. Cyclobenzaprine.
DB00531. Cyclophosphamide.
DB00091. Cyclosporine.
DB00987. Cytarabine.
DB08912. Dabrafenib.
DB09102. Daclatasvir.
DB01764. Dalfopristin.
DB01406. Danazol.
DB01219. Dantrolene.
DB06292. Dapagliflozin.
DB00250. Dapsone.
DB00496. Darifenacin.
DB01264. Darunavir.
DB09183. Dasabuvir.
DB01254. Dasatinib.
DB00694. Daunorubicin.
DB01609. Deferasirox.
DB11921. Deflazacort.
DB00705. Delavirdine.
DB01151. Desipramine.
DB06700. Desvenlafaxine.
DB01234. Dexamethasone.
DB04856. Dexloxiglumide.
DB00514. Dextromethorphan.
DB00647. Dextropropoxyphene.
DB00829. Diazepam.
DB00586. Diclofenac.
DB00485. Dicloxacillin.
DB00255. Diethylstilbestrol.
DB01396. Digitoxin.
DB00390. Digoxin.
DB01551. Dihydrocodeine.
DB00320. Dihydroergotamine.
DB00343. Diltiazem.
DB01093. Dimethyl sulfoxide.
DB00280. Disopyramide.
DB00822. Disulfiram.
DB01248. Docetaxel.
DB00204. Dofetilide.
DB00757. Dolasetron.
DB08930. Dolutegravir.
DB01184. Domperidone.
DB00843. Donepezil.
DB00869. Dorzolamide.
DB01142. Doxepin.
DB00997. Doxorubicin.
DB00254. Doxycycline.
DB00470. Dronabinol.
DB04855. Dronedarone.
DB01395. Drospirenone.
DB01126. Dutasteride.
DB01127. Econazole.
DB00625. Efavirenz.
DB00216. Eletriptan.
DB09039. Eliglustat.
DB09101. Elvitegravir.
DB00584. Enalapril.
DB08899. Enzalutamide.
DB00751. Epinastine.
DB00668. Epinephrine.
DB00700. Eplerenone.
DB00153. Ergocalciferol.
DB01049. Ergoloid mesylate.
DB01253. Ergonovine.
DB00696. Ergotamine.
DB00530. Erlotinib.
DB00199. Erythromycin.
DB01175. Escitalopram.
DB09119. Eslicarbazepine acetate.
DB00736. Esomeprazole.
DB01215. Estazolam.
DB00783. Estradiol.
DB01196. Estramustine.
DB00655. Estrone.
DB04574. Estrone sulfate.
DB00402. Eszopiclone.
DB00898. Ethanol.
DB00977. Ethinyl Estradiol.
DB00593. Ethosuximide.
DB01466. Ethylmorphine.
DB00294. Etonogestrel.
DB00773. Etoposide.
DB01628. Etoricoxib.
DB06414. Etravirine.
DB01590. Everolimus.
DB00990. Exemestane.
DB00973. Ezetimibe.
DB00426. Famciclovir.
DB00949. Felbamate.
DB01023. Felodipine.
DB01039. Fenofibrate.
DB00813. Fentanyl.
DB06702. Fesoterodine.
DB01216. Finasteride.
DB08868. Fingolimod.
DB04908. Flibanserin.
DB00301. Flucloxacillin.
DB00196. Fluconazole.
DB00180. Flunisolide.
DB01544. Flunitrazepam.
DB00324. Fluorometholone.
DB00472. Fluoxetine.
DB00690. Flurazepam.
DB00499. Flutamide.
DB08906. Fluticasone furoate.
DB00588. Fluticasone Propionate.
DB01095. Fluvastatin.
DB00176. Fluvoxamine.
DB01319. Fosamprenavir.
DB06717. Fosaprepitant.
DB01320. Fosphenytoin.
DB00947. Fulvestrant.
DB00674. Galantamine.
DB00317. Gefitinib.
DB01241. Gemfibrozil.
DB01067. Glipizide.
DB01016. Glyburide.
DB08909. Glycerol Phenylbutyrate.
DB00889. Granisetron.
DB00365. Grepafloxacin.
DB00400. Griseofulvin.
DB01170. Guanethidine.
DB01018. Guanfacine.
DB01218. Halofantrine.
DB00502. Haloperidol.
DB01159. Halothane.
DB01355. Hexobarbital.
DB05381. Histamine.
DB01275. Hydralazine.
DB00956. Hydrocodone.
DB00769. Hydrocortamate.
DB00741. Hydrocortisone.
DB00327. Hydromorphone.
DB06789. Hydroxyprogesterone caproate.
DB09053. Ibrutinib.
DB11737. Icotinib.
DB09054. Idelalisib.
DB01181. Ifosfamide.
DB04946. Iloperidone.
DB00619. Imatinib.
DB00458. Imipramine.
DB00724. Imiquimod.
DB05039. Indacaterol.
DB00808. Indapamide.
DB00224. Indinavir.
DB00332. Ipratropium bromide.
DB01029. Irbesartan.
DB00762. Irinotecan.
DB06636. Isavuconazonium.
DB00951. Isoniazid.
DB00883. Isosorbide Dinitrate.
DB01020. Isosorbide Mononitrate.
DB00270. Isradipine.
DB01167. Itraconazole.
DB09083. Ivabradine.
DB08820. Ivacaftor.
DB00602. Ivermectin.
DB04845. Ixabepilone.
DB09570. Ixazomib.
DB01321. Josamycin.
DB01221. Ketamine.
DB01587. Ketazolam.
DB06738. Ketobemidone.
DB01026. Ketoconazole.
DB00448. Lansoprazole.
DB01259. Lapatinib.
DB06685. Laquinimod.
DB09078. Lenvatinib.
DB00528. Lercanidipine.
DB01006. Letrozole.
DB01002. Levobupivacaine.
DB06282. Levocetirizine.
DB01137. Levofloxacin.
DB01227. Levomethadyl Acetate.
DB08918. Levomilnacipran.
DB00367. Levonorgestrel.
DB13139. Levosalbutamol.
DB00451. Levothyroxine.
DB00281. Lidocaine.
DB08882. Linagliptin.
DB00589. Lisuride.
DB08827. Lomitapide.
DB01206. Lomustine.
DB00836. Loperamide.
DB01601. Lopinavir.
DB00455. Loratadine.
DB04871. Lorcaserin.
DB00678. Losartan.
DB00227. Lovastatin.
DB08933. Luliconazole.
DB09280. Lumacaftor.
DB06708. Lumefantrine.
DB08815. Lurasidone.
DB08932. Macitentan.
DB04835. Maraviroc.
DB00643. Mebendazole.
DB00603. Medroxyprogesterone acetate.
DB00358. Mefloquine.
DB00814. Meloxicam.
DB01071. Mequitazine.
DB04817. Metamizole.
DB00333. Methadone.
DB04833. Methaqualone.
DB00703. Methazolamide.
DB00763. Methimazole.
DB00553. Methoxsalen.
DB01028. Methoxyflurane.
DB09543. Methyl salicylate.
DB00353. Methylergometrine.
DB00959. Methylprednisolone.
DB06710. Methyltestosterone.
DB00916. Metronidazole.
DB01011. Metyrapone.
DB00379. Mexiletine.
DB06148. Mianserin.
DB01388. Mibefradil.
DB01110. Miconazole.
DB00683. Midazolam.
DB00834. Mifepristone.
DB08893. Mirabegron.
DB00370. Mirtazapine.
DB00648. Mitotane.
DB01204. Mitoxantrone.
DB00745. Modafinil.
DB00471. Montelukast.
DB00295. Morphine.
DB00688. Mycophenolate mofetil.
DB00607. Nafcillin.
DB09049. Naloxegol.
DB01183. Naloxone.
DB00731. Nateglinide.
DB01149. Nefazodone.
DB00220. Nelfinavir.
DB09048. Netupitant.
DB00238. Nevirapine.
DB00622. Nicardipine.
DB02701. Nicotinamide.
DB00184. Nicotine.
DB01115. Nifedipine.
DB04868. Nilotinib.
DB06712. Nilvadipine.
DB00393. Nimodipine.
DB00401. Nisoldipine.
DB01595. Nitrazepam.
DB01054. Nitrendipine.
DB00435. Nitric Oxide.
DB00717. Norethisterone.
DB01059. Norfloxacin.
DB00957. Norgestimate.
DB09389. Norgestrel.
DB00540. Nortriptyline.
DB00334. Olanzapine.
DB09074. Olaparib.
DB00768. Olopatadine.
DB00338. Omeprazole.
DB00904. Ondansetron.
DB04911. Oritavancin.
DB01083. Orlistat.
DB01173. Orphenadrine.
DB09330. Osimertinib.
DB04938. Ospemifene.
DB00842. Oxazepam.
DB00776. Oxcarbazepine.
DB00239. Oxiconazole.
DB01062. Oxybutynin.
DB00497. Oxycodone.
DB01192. Oxymorphone.
DB01229. Paclitaxel.
DB09073. Palbociclib.
DB01267. Paliperidone.
DB00377. Palonosetron.
DB06603. Panobinostat.
DB00213. Pantoprazole.
DB00617. Paramethadione.
DB01384. Paramethasone.
DB08439. Parecoxib.
DB00910. Paricalcitol.
DB09297. Paritaprevir.
DB06589. Pazopanib.
DB00082. Pegvisomant.
DB00738. Pentamidine.
DB00312. Pentobarbital.
DB08883. Perampanel.
DB01186. Pergolide.
DB01074. Perhexiline.
DB04930. Permethrin.
DB00850. Perphenazine.
DB00454. Pethidine.
DB03783. Phenacetin.
DB00780. Phenelzine.
DB01174. Phenobarbital.
DB00925. Phenoxybenzamine.
DB00946. Phenprocoumon.
DB00812. Phenylbutazone.
DB00252. Phenytoin.
DB01085. Pilocarpine.
DB00337. Pimecrolimus.
DB01100. Pimozide.
DB01132. Pioglitazone.
DB01621. Pipotiazine.
DB04977. Plitidepsin.
DB01179. Podofilox.
DB08910. Pomalidomide.
DB08901. Ponatinib.
DB01263. Posaconazole.
DB05478. Pradefovir Mesylate.
DB01411. Pranlukast.
DB01708. Prasterone.
DB06209. Prasugrel.
DB00175. Pravastatin.
DB01588. Prazepam.
DB01058. Praziquantel.
DB00860. Prednisolone.
DB00635. Prednisone.
DB01087. Primaquine.
DB00794. Primidone.
DB01032. Probenecid.
DB00433. Prochlorperazine.
DB00396. Progesterone.
DB01131. Proguanil.
DB00420. Promazine.
DB01182. Propafenone.
DB00818. Propofol.
DB09396. Propoxyphene napsylate.
DB00571. Propranolol.
DB00339. Pyrazinamide.
DB00545. Pyridostigmine.
DB01589. Quazepam.
DB01224. Quetiapine.
DB01103. Quinacrine.
DB00908. Quinidine.
DB00468. Quinine.
DB01369. Quinupristin.
DB01129. Rabeprazole.
DB00481. Raloxifene.
DB00980. Ramelteon.
DB00863. Ranitidine.
DB00243. Ranolazine.
DB00234. Reboxetine.
DB08896. Regorafenib.
DB00912. Repaglinide.
DB02709. Resveratrol.
DB01256. Retapamulin.
DB00615. Rifabutin.
DB04934. Rifalazil.
DB01045. Rifampicin.
DB01201. Rifapentine.
DB01220. Rifaximin.
DB08864. Rilpivirine.
DB06155. Rimonabant.
DB08931. Riociguat.
DB00734. Risperidone.
DB00503. Ritonavir.
DB06228. Rivaroxaban.
DB00989. Rivastigmine.
DB00533. Rofecoxib.
DB09291. Rolapitant.
DB01301. Rolitetracycline.
DB06176. Romidepsin.
DB00268. Ropinirole.
DB00296. Ropivacaine.
DB01098. Rosuvastatin.
DB05271. Rotigotine.
DB00778. Roxithromycin.
DB12332. Rucaparib.
DB06201. Rufinamide.
DB01698. Rutin.
DB08877. Ruxolitinib.
DB06654. Safinamide.
DB01001. Salbutamol.
DB00938. Salmeterol.
DB01232. Saquinavir.
DB06335. Saxagliptin.
DB01037. Selegiline.
DB11362. Selexipag.
DB06144. Sertindole.
DB01104. Sertraline.
DB01236. Sevoflurane.
DB01105. Sibutramine.
DB00203. Sildenafil.
DB06207. Silodosin.
DB06290. Simeprevir.
DB00641. Simvastatin.
DB00877. Sirolimus.
DB01261. Sitagliptin.
DB06268. Sitaxentan.
DB01591. Solifenacin.
DB09143. Sonidegib.
DB00398. Sorafenib.
DB06145. Spiramycin.
DB01323. St. John's Wort.
DB00708. Sufentanil.
DB00359. Sulfadiazine.
DB01015. Sulfamethoxazole.
DB00259. Sulfanilamide.
DB01138. Sulfinpyrazone.
DB00263. Sulfisoxazole.
DB01268. Sunitinib.
DB00864. Tacrolimus.
DB00820. Tadalafil.
DB00675. Tamoxifen.
DB00706. Tamsulosin.
DB01349. Tasosartan.
DB05521. Telaprevir.
DB00976. Telithromycin.
DB00231. Temazepam.
DB00853. Temozolomide.
DB06287. Temsirolimus.
DB00444. Teniposide.
DB00857. Terbinafine.
DB00342. Terfenadine.
DB04905. Tesmilifene.
DB00624. Testosterone.
DB00759. Tetracycline.
DB00277. Theophylline.
DB00599. Thiopental.
DB04572. Thiotepa.
DB00906. Tiagabine.
DB08816. Ticagrelor.
DB00208. Ticlopidine.
DB00911. Tinidazole.
DB01007. Tioconazole.
DB01409. Tiotropium.
DB00932. Tipranavir.
DB08895. Tofacitinib.
DB08811. Tofisopam.
DB01036. Tolterodine.
DB06212. Tolvaptan.
DB00273. Topiramate.
DB01030. Topotecan.
DB00539. Toremifene.
DB05109. Trabectedin.
DB00193. Tramadol.
DB08911. Trametinib.
DB00752. Tranylcypromine.
DB05773. Trastuzumab emtansine.
DB00656. Trazodone.
DB00755. Tretinoin.
DB00620. Triamcinolone.
DB00897. Triazolam.
DB00347. Trimethadione.
DB00440. Trimethoprim.
DB00726. Trimipramine.
DB00197. Troglitazone.
DB01361. Troleandomycin.
DB06267. Udenafil.
DB08867. Ulipristal.
DB00580. Valdecoxib.
DB00313. Valproic Acid.
DB05294. Vandetanib.
DB03701. Vanoxerine.
DB00862. Vardenafil.
DB08881. Vemurafenib.
DB11581. Venetoclax.
DB00285. Venlafaxine.
DB00661. Verapamil.
DB09082. Vilanterol.
DB06684. Vilazodone.
DB00570. Vinblastine.
DB00541. Vincristine.
DB00309. Vindesine.
DB00361. Vinorelbine.
DB08828. Vismodegib.
DB00582. Voriconazole.
DB09068. Vortioxetine.
DB00682. Warfarin.
DB01392. Yohimbine.
DB00549. Zafirlukast.
DB00943. Zalcitabine.
DB00962. Zaleplon.
DB00495. Zidovudine.
DB00744. Zileuton.
DB00246. Ziprasidone.
DB00425. Zolpidem.
DB04828. Zomepirac.
DB00909. Zonisamide.
DB01198. Zopiclone.

Polymorphism and mutation databases


PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00000517862 – 503Cytochrome P450 3A4Add BLAST502

Post-translational modificationi

Polyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro).1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases


PTM databases



Tissue specificityi

Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056). According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level).6 Publications


By glucocorticoids. Also induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Gene expression databases

ExpressionAtlasiP08684. baseline and differential.
GenevisibleiP08684. HS.

Organism-specific databases



GO - Molecular functioni

  • enzyme binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi107948. 16 interactors.
IntActiP08684. 2 interactors.

Chemistry databases



Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi23 – 26Combined sources4
Helixi29 – 31Combined sources3
Helixi32 – 36Combined sources5
Turni45 – 47Combined sources3
Helixi50 – 55Combined sources6
Helixi57 – 68Combined sources12
Beta strandi70 – 76Combined sources7
Beta strandi79 – 84Combined sources6
Helixi87 – 94Combined sources8
Turni95 – 101Combined sources7
Helixi112 – 116Combined sources5
Turni118 – 120Combined sources3
Helixi123 – 133Combined sources11
Helixi134 – 137Combined sources4
Helixi139 – 164Combined sources26
Turni165 – 167Combined sources3
Helixi172 – 189Combined sources18
Turni195 – 197Combined sources3
Helixi202 – 207Combined sources6
Beta strandi209 – 212Combined sources4
Beta strandi215 – 217Combined sources3
Helixi218 – 225Combined sources8
Helixi227 – 229Combined sources3
Helixi230 – 235Combined sources6
Helixi243 – 258Combined sources16
Turni259 – 263Combined sources5
Helixi271 – 277Combined sources7
Beta strandi284 – 286Combined sources3
Helixi292 – 323Combined sources32
Helixi325 – 338Combined sources14
Turni340 – 342Combined sources3
Helixi347 – 351Combined sources5
Helixi354 – 366Combined sources13
Beta strandi369 – 371Combined sources3
Beta strandi373 – 376Combined sources4
Beta strandi381 – 383Combined sources3
Beta strandi386 – 388Combined sources3
Beta strandi393 – 396Combined sources4
Helixi398 – 402Combined sources5
Turni405 – 407Combined sources3
Beta strandi408 – 410Combined sources3
Helixi416 – 419Combined sources4
Turni421 – 423Combined sources3
Helixi424 – 426Combined sources3
Turni429 – 431Combined sources3
Helixi438 – 440Combined sources3
Helixi445 – 462Combined sources18
Beta strandi463 – 467Combined sources5
Beta strandi469 – 471Combined sources3
Beta strandi479 – 482Combined sources4
Beta strandi485 – 487Combined sources3
Beta strandi490 – 495Combined sources6

3D structure databases

Select the link destinations:
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum

Miscellaneous databases


Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0158. Eukaryota.
COG2124. LUCA.

Family and domain databases

InterProiView protein in InterPro
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR008072. Cyt_P450_E_CYP3A.
IPR002402. Cyt_P450_E_grp-II.
PfamiView protein in Pfam
PF00067. p450. 1 hit.
PRINTSiPR00464. EP450II.
PR01689. EP450IICYP3A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiView protein in PROSITE
PS00086. CYTOCHROME_P450. 1 hit.


Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08684-1 [UniParc]FASTAAdd to basket

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Mass (Da):57,343
Last modified:January 23, 2007 - v4

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3L → V in AAA35747 (PubMed:2463251).Curated1
Sequence conflicti12W → R in AAF13598 (Ref. 9) Curated1
Sequence conflicti72W → C in AAA35747 (PubMed:2463251).Curated1
Sequence conflicti92T → L in BAA00001 (PubMed:3460094).Curated1
Sequence conflicti92T → L in AAA35742 (PubMed:3460094).Curated1
Sequence conflicti106R → E in BAA00001 (PubMed:3460094).Curated1
Sequence conflicti106R → E in AAA35742 (PubMed:3460094).Curated1
Sequence conflicti164A → R in BAA00001 (PubMed:3460094).Curated1
Sequence conflicti164A → R in AAA35742 (PubMed:3460094).Curated1
Sequence conflicti187T → S in BAA00001 (PubMed:3460094).Curated1
Sequence conflicti187T → S in AAA35742 (PubMed:3460094).Curated1
Sequence conflicti193I → V in BAA00001 (PubMed:3460094).Curated1
Sequence conflicti193I → V in AAA35742 (PubMed:3460094).Curated1
Sequence conflicti203F → L in BAA00001 (PubMed:3460094).Curated1
Sequence conflicti203F → L in AAA35742 (PubMed:3460094).Curated1
Sequence conflicti224 – 225TV → I in AAA35747 (PubMed:2463251).Curated2
Sequence conflicti279Q → HK in BAA00001 (PubMed:3460094).Curated1
Sequence conflicti279Q → HK in AAA35742 (PubMed:3460094).Curated1
Sequence conflicti307Y → C in AAF13598 (Ref. 9) Curated1
Sequence conflicti392V → W in BAA00001 (PubMed:3460094).Curated1
Sequence conflicti392V → W in AAA35742 (PubMed:3460094).Curated1
Sequence conflicti392V → W in AAA35744 (PubMed:3464943).Curated1
Sequence conflicti392V → W in CAA30944 (PubMed:2563251).Curated1
Sequence conflicti392V → W in AAA35747 (PubMed:2463251).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01159715L → P in allele CYP3A4*14. 1 PublicationCorresponds to variant dbSNP:rs12721634Ensembl.1
Natural variantiVAR_01159856G → D in allele CYP3A4*7. 1 PublicationCorresponds to variant dbSNP:rs56324128Ensembl.1
Natural variantiVAR_03754796K → E. Corresponds to variant dbSNP:rs3091339Ensembl.1
Natural variantiVAR_011599118I → V in allele CYP3A4*4. 1 PublicationCorresponds to variant dbSNP:rs55951658Ensembl.1
Natural variantiVAR_011600130R → Q in allele CYP3A4*8. 1 PublicationCorresponds to variant dbSNP:rs72552799Ensembl.1
Natural variantiVAR_011601162R → Q in allele CYP3A4*15. 2 PublicationsCorresponds to variant dbSNP:rs4986907Ensembl.1
Natural variantiVAR_011602170V → I in allele CYP3A4*9. 1 PublicationCorresponds to variant dbSNP:rs72552798Ensembl.1
Natural variantiVAR_011603174D → H in allele CYP3A4*10. 3 PublicationsCorresponds to variant dbSNP:rs4986908Ensembl.1
Natural variantiVAR_011604185T → S in allele CYP3A4*16. 2 PublicationsCorresponds to variant dbSNP:rs12721627Ensembl.1
Natural variantiVAR_014322189F → S in allele CYP3A4*17; exhibits lower turnover numbers for testosterone and chlorpyrifos. 1 PublicationCorresponds to variant dbSNP:rs4987161Ensembl.1
Natural variantiVAR_011605218P → R in allele CYP3A4*5. 1 PublicationCorresponds to variant dbSNP:rs55901263Ensembl.1
Natural variantiVAR_008363222S → P in allele CYP3A4*2; exhibits a lower intrinsic clearance toward nifedipine. 1 PublicationCorresponds to variant dbSNP:rs55785340Ensembl.1
Natural variantiVAR_037548252S → A1 PublicationCorresponds to variant dbSNP:rs3208363Ensembl.1
Natural variantiVAR_014323293L → P in allele CYP3A4*18; exhibits higher turnover numbers for testosterone and chlorpyrifos. 2 PublicationsCorresponds to variant dbSNP:rs28371759Ensembl.1
Natural variantiVAR_037549349T → N. Corresponds to variant dbSNP:rs10250778Ensembl.1
Natural variantiVAR_011606363T → M in allele CYP3A4*11; unstable form. 1 PublicationCorresponds to variant dbSNP:rs67784355Ensembl.1
Natural variantiVAR_011607373L → F in allele CYP3A4*12; has an altered testosterone hydroxylase activity. 2 PublicationsCorresponds to variant dbSNP:rs12721629Ensembl.1
Natural variantiVAR_011608416P → L in allele CYP3A4*13; lack of expression. 1 PublicationCorresponds to variant dbSNP:rs4986909Ensembl.1
Natural variantiVAR_037550431I → T2 PublicationsCorresponds to variant dbSNP:rs1041988Ensembl.1
Natural variantiVAR_008364445M → T in allele CYP3A4*3. 2 PublicationsCorresponds to variant dbSNP:rs4986910Ensembl.1
Natural variantiVAR_014324467P → S in allele CYP3A4*19. 1 PublicationCorresponds to variant dbSNP:rs4986913Ensembl.1

Sequence databases

Select the link destinations:
Links Updated
D00003 mRNA. Translation: BAA00001.1.
M13785 mRNA. Translation: AAA35742.1.
M18907 mRNA. Translation: AAA35745.1.
M14096 mRNA. Translation: AAA35744.1.
X12387 mRNA. Translation: CAA30944.1.
J04449 mRNA. Translation: AAA35747.1.
AF182273 mRNA. Translation: AAF13598.1.
AF280107 Genomic DNA. Translation: AAG32290.1.
AF209389 Genomic DNA. Translation: AAF21034.1.
RefSeqiNP_001189784.1. NM_001202855.2.
NP_059488.2. NM_017460.5.

Genome annotation databases

EnsembliENST00000336411; ENSP00000337915; ENSG00000160868.

Keywords - Coding sequence diversityi


Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCP3A4_HUMAN
AccessioniPrimary (citable) accession number: P08684
Secondary accession number(s): P05184, Q16757, Q9UK50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 23, 2007
Last modified: June 7, 2017
This is version 199 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.


Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome


  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families