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Protein

Citrate synthase, peroxisomal

Gene

CIT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.
Present with 2310 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase, mitochondrial (CIT1), Citrate synthase 3, mitochondrial (CIT3), Citrate synthase, peroxisomal (CIT2)
  2. Aconitate hydratase, mitochondrial (ACO1)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei293PROSITE-ProRule annotation1
Active sitei339PROSITE-ProRule annotation1
Active sitei394PROSITE-ProRule annotation1

GO - Molecular functioni

  • citrate (Si)-synthase activity Source: SGD

GO - Biological processi

  • citrate metabolic process Source: SGD
  • glutamate biosynthetic process Source: SGD
  • glyoxylate cycle Source: SGD
  • tricarboxylic acid cycle Source: UniProtKB-UniPathway

Keywordsi

Molecular functionTransferase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

BioCyciYEAST:YCR005C-MONOMER
ReactomeiR-SCE-71403 Citric acid cycle (TCA cycle)
UniPathwayiUPA00223; UER00717

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase, peroxisomal (EC:2.3.3.16)
Gene namesi
Name:CIT2
Ordered Locus Names:YCR005C
ORF Names:YCR043, YCR5C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR005C
SGDiS000000598 CIT2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1741170

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001699841 – 460Citrate synthase, peroxisomalAdd BLAST460

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei21PhosphoserineCombined sources1
Cross-linki218Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki239Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki354Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki385Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP08679
PaxDbiP08679
PRIDEiP08679

PTM databases

iPTMnetiP08679

Interactioni

Protein-protein interaction databases

BioGridi30983, 107 interactors
DIPiDIP-1259N
IntActiP08679, 5 interactors
MINTiP08679
STRINGi4932.YCR005C

Structurei

3D structure databases

ProteinModelPortaliP08679
SMRiP08679
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi458 – 460Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000006813
HOGENOMiHOG000130831
InParanoidiP08679
KOiK01647
OMAiNCYPVLF
OrthoDBiEOG092C1UDR

Family and domain databases

Gene3Di1.10.230.10, 1 hit
1.10.580.10, 2 hits
InterProiView protein in InterPro
IPR016142 Citrate_synth-like_lrg_a-sub
IPR016143 Citrate_synth-like_sm_a-sub
IPR002020 Citrate_synthase
IPR019810 Citrate_synthase_AS
IPR010109 Citrate_synthase_euk
IPR036969 Citrate_synthase_sf
PANTHERiPTHR11739 PTHR11739, 1 hit
PfamiView protein in Pfam
PF00285 Citrate_synt, 1 hit
PRINTSiPR00143 CITRTSNTHASE
SUPFAMiSSF48256 SSF48256, 1 hit
TIGRFAMsiTIGR01793 cit_synth_euk, 1 hit
PROSITEiView protein in PROSITE
PS00480 CITRATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

P08679-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVPYLNSNR NVASYLQSNS SQEKTLKERF SEIYPIHAQD VRQFVKEHGK
60 70 80 90 100
TKISDVLLEQ VYGGMRGIPG SVWEGSVLDP EDGIRFRGRT IADIQKDLPK
110 120 130 140 150
AKGSSQPLPE ALFWLLLTGE VPTQAQVENL SADLMSRSEL PSHVVQLLDN
160 170 180 190 200
LPKDLHPMAQ FSIAVTALES ESKFAKAYAQ GISKQDYWSY TFEDSLDLLG
210 220 230 240 250
KLPVIAAKIY RNVFKDGKMG EVDPNADYAK NLVNLIGSKD EDFVDLMRLY
260 270 280 290 300
LTIHSDHEGG NVSAHTSHLV GSALSSPYLS LASGLNGLAG PLHGRANQEV
310 320 330 340 350
LEWLFALKEE VNDDYSKDTI EKYLWDTLNS GRVIPGYGHA VLRKTDPRYM
360 370 380 390 400
AQRKFAMDHF PDYELFKLVS SIYEVAPGVL TEHGKTKNPW PNVDAHSGVL
410 420 430 440 450
LQYYGLKESS FYTVLFGVSR AFGILAQLIT DRAIGASIER PKSYSTEKYK
460
ELVKNIESKL
Length:460
Mass (Da):51,413
Last modified:January 1, 1988 - v1
Checksum:iAB2F66AD9A9399EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11113 Genomic DNA Translation: CAA77442.1
M14686 Genomic DNA Translation: AAA34497.1
X59720 Genomic DNA Translation: CAA42342.1
AY692837 Genomic DNA Translation: AAT92856.1
M54982 Genomic DNA Translation: AAA34498.1
BK006937 Genomic DNA Translation: DAA07484.1
PIRiA25393 YKBYC
RefSeqiNP_009931.1, NM_001178718.1

Genome annotation databases

EnsemblFungiiCAA42342; CAA42342; CAA42342
YCR005C; YCR005C; YCR005C
GeneIDi850361
KEGGisce:YCR005C

Similar proteinsi

Entry informationi

Entry nameiCISY2_YEAST
AccessioniPrimary (citable) accession number: P08679
Secondary accession number(s): D6VR15
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: May 23, 2018
This is version 173 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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