P08668 (GP_HANTV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Envelope glycoprotein
Short name=GP

Alternative name(s):
M polyprotein

Cleaved into the following 2 chains:

1. Glycoprotein G1
2. Glycoprotein G2

Gene names

Name:

GP

Organism

Hantaan virus (strain 76-118) (Korean hemorrhagic fever virus) [Reference proteome]

Taxonomic identifier

11602 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Bunyaviridae › Hantavirus › Hantaan virus

Virus host

Apodemus agrarius (Eurasian field mouse) [TaxID: 39030]
Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	1135 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to host cell receptors. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Also promote fusion of viral membrane with host endosomal membrane after endocytosis of the virion. G1 contains an ITAM motif which is likely to dysregulate normal immune and endothelial cell responses and contribute to virus pathogenesis By similarity. Ref.4
Subunit structure	G1 and G2 interacts with each other By similarity.
Subcellular location	Glycoprotein G1: Virion membrane Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential. Host endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Note: Interaction between G1 and G2 is essential for proper targeting of G1 to the Golgi complex, where virion budding occurs By similarity. Glycoprotein G2: Virion membrane Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential.
Post-translational modification	Specific enzymatic cleavages in vivo yield mature proteins including glycoprotein G1 and glycoprotein G2.
Miscellaneous	It was not possible to precisely define the C-terminus of G1 and G2 mature glycoproteins.
Sequence similarities	Belongs to the hantavirus envelope glycoprotein family. Contains 1 ITAM domain.

Ontologies

Keywords
Biological process	Fusion of virus membrane with host endosomal membrane Fusion of virus membrane with host membrane Host-virus interaction Inhibition of host IFN-mediated response initiation by virus Inhibition of host TRAFs by virus Inhibition of host innate immune response by virus Viral attachment to host cell Viral immunoevasion Viral penetration into host cytoplasm Virus endocytosis by host Virus entry into host cell
Cellular component	Host Golgi apparatus Host endoplasmic reticulum Host membrane Membrane Viral envelope protein Virion
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	signal transduction Inferred from electronic annotation. Source: InterPro suppression by virus of host TRAF activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host innate immune response Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon production Inferred from electronic annotation. Source: UniProtKB-KW viral attachment to host cell Inferred from electronic annotation. Source: UniProtKB-KW viral entry into host cell via endocytosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell host cell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18
	Chain	19 – 1135	1117	Envelope glycoprotein		PRO_0000036815
	Chain	19 – ?648	630	Glycoprotein G1		PRO_0000036816
	Chain	649 – ?1126	478	Glycoprotein G2		PRO_0000036817
Regions
	Topological domain	19 – 485	467	Lumenal Potential
	Transmembrane	486 – 506	21	Helical; Potential
	Topological domain	507 – 648	142	Cytoplasmic Potential
	Topological domain	649 – 1105	457	Lumenal Potential
	Transmembrane	1106 – 1126	21	Helical; Potential
	Topological domain	1127 – 1135	9	Cytoplasmic Potential
	Domain	611 – 634	24	ITAM
Sites
	Site	645 – 646	2	Cleavage; by host signal peptidase
Amino acid modifications
	Glycosylation	134	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	235	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	347	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	399	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	928	1	N-linked (GlcNAc...); by host Potential
Experimental info
	Sequence conflict	37	1	E → G in CAA68456. Ref.2
	Sequence conflict	64	1	N → S in CAA68456. Ref.2
	Sequence conflict	173	1	S → T in CAA68456. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P08668 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 8E40B8EA68EA62FA	FASTA	1,135	126,421
	10 20 30 40 50 60 MGIWKWLVMA SLVWPVLTLR NVYDMKIECP HTVSFGENSV IGYVELPPVP LADTAQMVPE 70 80 90 100 110 120 SSCNMDNHQS LNTITKYTQV SWRGKADQSQ SSQNSFETVS TEVDLKGTCV LKHKMVEESY 130 140 150 160 170 180 RSRKSVTCYD LSCNSTYCKP TLYMIVPIHA CNMMKSCLIA LGPYRVQVVY ERSYCMTGVL 190 200 210 220 230 240 IEGKCFVPDQ SVVSIIKHGI FDIASVHIVC FFVAVKGNTY KIFEQVKKSF ESTCNDTENK 250 260 270 280 290 300 VQGYYICIVG GNSAPIYVPT LDDFRSMEAF TGIFRSPHGE DHDLAGEEIA SYSIVGPANA 310 320 330 340 350 360 KVPHSASSDT LSLIAYSGIP SYSSLSILTS STEAKHVFSP GLFPKLNHTN CDKSAIPLIW 370 380 390 400 410 420 TGMIDLPGYY EAVHPCTVFC VLSGPGASCE AFSEGGIFNI TSPMCLVSKQ NRFRLTEQQV 430 440 450 460 470 480 NFVCQRVDMD IVVYCNGQRK VILTKTLVIG QCIYTITSLF SLLPGVAHSI AVELCVPGFH 490 500 510 520 530 540 GWATAALLVT FCFGWVLIPA ITFIILTVLK FIANIFHTSN QENRLKSVLR KIKEEFEKTK 550 560 570 580 590 600 GSMVCDVCKY ECETYKELKA HGVSCPQSQC PYCFTHCEPT EAAFQAHYKV CQVTHRFRDD 610 620 630 640 650 660 LKKTVTPQNF TPGCYRTLNL FRYKSRCYIF TMWIFLLVLE SILWAASASE TPLTPVWNDN 670 680 690 700 710 720 AHGVGSVPMH TDLELDFSLT SSSKYTYRRK LTNPLEEAQS IDLHIEIEEQ TIGVDVHALG 730 740 750 760 770 780 HWFDGRLNLK TSFHCYGACT KYEYPWHTAK CHYERDYQYE TSWGCNPSDC PGVGTGCTAC 790 800 810 820 830 840 GLYLDQLKPV GSAYKIITIR YSRRVCVQFG EENLCKIIDM NDCFVSRHVK VCIIGTVSKF 850 860 870 880 890 900 SQGDTLLFFG PLEGGGLIFK HWCTSTCQFG DPGDIMSPRD KGFLCPEFPG SFRKKCNFAT 910 920 930 940 950 960 TPICEYDGNM VSGYKKVMAT IDSFQSFNTS TMHFTDERIE WKDPDGMLRD HINILVTKDI 970 980 990 1000 1010 1020 DFDNLGENPC KIGLQTSSIE GAWGSGVGFT LTCLVSLTEC PTFLTSIKAC DKAICYGAES 1030 1040 1050 1060 1070 1080 VTLTRGQNTV KVSGKGGHSG STFRCCHGED CSQIGLHAAA PHLDKVNGIS EIENSKVYDD 1090 1100 1110 1120 1130 GAPQCGIKCW FVKSGEWISG IFSGNWIVLI VLCVFLLFSL VLLSILCPVR KHKKS

References

[1]	"Hantaan virus M RNA: coding strategy, nucleotide sequence, and gene order." Schmaljohn C.S., Schmaljohn A.L., Dalrymple J.M. Virology 157:31-39(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Nucleotide sequence of the M segment of the genomic RNA of Hantaan virus 76-118." Yoo D., Kang C.Y. Nucleic Acids Res. 15:6299-6299(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]	"The Hantaan virus glycoprotein precursor is cleaved at the conserved pentapeptide WAASA." Loeber C., Anheier B., Lindow S., Klenk H.-D., Feldmann H. Virology 289:224-229(2001) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE BY SIGNAL PEPTIDASE.
[4]	"Hantaan virus enters cells by clathrin-dependent receptor-mediated endocytosis." Jin M., Park J., Lee S., Park B., Shin J., Song K.J., Ahn T.I., Hwang S.Y., Ahn B.Y., Ahn K. Virology 294:60-69(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M14627 Genomic RNA. Translation: AAA43836.1. Y00386 mRNA. Translation: CAA68456.1.
PIR	GNVUHV. A26348. GNVUH7. A29382.
RefSeq	NP_941978.1. NC_005219.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016402. Envelope_gylcoprot_Hantavirus. IPR002534. Hanta_G1. IPR002532. Hanta_G2. IPR012316. ITAM_motif_hantavir-typ. [Graphical view]
Pfam	PF01567. Hanta_G1. 1 hit. PF01561. Hanta_G2. 1 hit. PF10538. ITAM_Cys-rich. 1 hit. [Graphical view]
PIRSF	PIRSF003945. M_poly_HantaV. 1 hit.
ProDom	PD001813. Hanta_G2. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS51055. ITAM_1. False negative. PS51056. ITAM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GP_HANTV

Accession

Primary (citable) accession number: P08668

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	April 3, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program