Lysine-sensitive aspartokinase 3 - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P08660 (AK3_ECOLI)

Last modified June 16, 2009. Version 86. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Lysine-sensitive aspartokinase 3
    EC=2.7.2.4
Alternative name(s):
    Lysine-sensitive aspartokinase III
    Aspartate kinase III

Gene names

Name:	lysC
Synonyms:	apk
Ordered Locus Names:	b4024, JW3984

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
Enzyme regulation	Synthesis and activity are sensitive to lysine, which is one of the end metabolites of the aspartic acid family branched pathway.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 1/4.
Subunit structure	Homodimer.
Miscellaneous	Aspartokinases I and II also catalyze the same reaction(s).
Sequence similarities	Belongs to the aspartokinase family. Contains 1 ACT domain.

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Ontologies

Keywords
Biological process	Amino-acid biosynthesis Lysine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	lysine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW amino acid binding Inferred from electronic annotation. Source: InterPro aspartate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 449

449

Lysine-sensitive aspartokinase 3

PRO_0000066676

Regions

Domain

309 – 386

ACT

Region

1 – 245

245

Aspartokinase

Region

246 – 449

204

Interface

Experimental info

Sequence conflict

G → C in AAA24095. Ref.1

Sequence conflict

401

G → A in AAA24095. Ref.1

Secondary structure

449

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P08660-1 [UniParc].

Last modified October 1, 1993. Version 2.
Checksum: 5B41CE3A6E4D984B
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FASTA

449

48,532

        10         20         30         40         50         60 
MSEIVVSKFG GTSVADFDAM NRSADIVLSD ANVRLVVLSA SAGITNLLVA LAEGLEPGER 

        70         80         90        100        110        120 
FEKLDAIRNI QFAILERLRY PNVIREEIER LLENITVLAE AAALATSPAL TDELVSHGEL 

       130        140        150        160        170        180 
MSTLLFVEIL RERDVQAQWF DVRKVMRTND RFGRAEPDIA ALAELAALQL LPRLNEGLVI 

       190        200        210        220        230        240 
TQGFIGSENK GRTTTLGRGG SDYTAALLAE ALHASRVDIW TDVPGIYTTD PRVVSAAKRI 

       250        260        270        280        290        300 
DEIAFAEAAE MATFGAKVLH PATLLPAVRS DIPVFVGSSK DPRAGGTLVC NKTENPPLFR 

       310        320        330        340        350        360 
ALALRRNQTL LTLHSLNMLH SRGFLAEVFG ILARHNISVD LITTSEVSVA LTLDTTGSTS 

       370        380        390        400        410        420 
TGDTLLTQSL LMELSALCRV EVEEGLALVA LIGNDLSKAC GVGKEVFGVL EPFNIRMICY 

       430        440 
GASSHNLCFL VPGEDAEQVV QKLHSNLFE

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of lysC gene encoding the lysine-sensitive aspartokinase III of Escherichia coli K12. Evolutionary pathway leading to three isofunctional enzymes." Cassan M., Parsot C., Cohen G.N., Patte J.-C. J. Biol. Chem. 261:1052-1057(1986) [PubMed: 3003049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Nucleotide sequence of the promoter region of the E. coli lysC gene." Cassan M., Ronceray J., Patte J.-C. Nucleic Acids Res. 11:6157-6166(1983) [PubMed: 6312411] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-20.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

M11812 Genomic DNA. Translation: AAA24095.1.
U00006 Genomic DNA. Translation: AAC43118.1.
U00096 Genomic DNA. Translation: AAC76994.1.
AP009048 Genomic DNA. Translation: BAE78026.1.
X00008 Genomic DNA. Translation: CAA24910.1. Sequence problems.

PIR

KIECD3. G65209.

RefSeq

AP_004525.1.
NP_418448.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2J0W	X-ray	2.50	A	1-449	[»]
2J0X	X-ray	2.80	A/B	1-449	[»]

ModBase

Search...

Genome annotation databases

GeneID

948531.

GenomeReviews

Gene locus JW3984 in contig AP009048_GR.
Gene locus b4024 in contig U00096_GR.

KEGG

ecj:JW3984.
eco:b4024.

Organism-specific databases

EchoBASE

EB0545.

EcoGene

EG10550. lysC.

CMR

Search...

Phylogenomic databases

HOGENOM

P08660.

OMA

P08660. GFIGADE.

Enzyme and pathway databases

BioCyc

EcoCyc:ASPKINIII-MON.
MetaCyc:ASPKINIII-MON.

Family and domain databases

InterPro

IPR002912. ACT_bd.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR005260. Asp_kin_monofn.
IPR001341. Asp_kin_reg.
IPR018042. Aspartate_kinase_CS.
[Graphical view]

Gene3D

G3DSA:3.40.1160.10. Aa_kinase. 1 hit.

Pfam

PF00696. AA_kinase. 1 hit.
PF01842. ACT. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00656. asp_kin_monofn. 1 hit.
TIGR00657. asp_kinases. 1 hit.

PROSITE

PS00324. ASPARTOKINASE. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

AK3_ECOLI

Accession

Primary (citable) accession number: P08660
Secondary accession number(s): Q2M6T0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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