ID LUCI_PHOPY Reviewed; 550 AA. AC P08659; Q27755; DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot. DT 01-JAN-1988, sequence version 1. DT 03-MAY-2023, entry version 130. DE RecName: Full=Luciferin 4-monooxygenase; DE Short=Luciferase; DE EC=1.13.12.7 {ECO:0000250|UniProtKB:Q26304}; OS Photinus pyralis (Common eastern firefly) (Lampyris pyralis). OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Coleoptera; Polyphaga; Elateriformia; Elateroidea; OC Lampyridae; Lampyrinae; Photinus. OX NCBI_TaxID=7054; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX PubMed=3821727; DOI=10.1128/mcb.7.2.725-737.1987; RA de Wet J.R., Wood K.V., Deluca M., Helinski D.R., Subramani S.; RT "Firefly luciferase gene: structure and expression in mammalian cells."; RL Mol. Cell. Biol. 7:725-737(1987). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RA Croizier G.M.J.; RT "Construction and utilization of an Autographa californica nuclear RT polyhedrosis virus vector with a unique cloning site: expression of genes RT amplified by the polymerase chain reaction."; RL Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases. RN [3] RP SUBCELLULAR LOCATION. RX PubMed=3554235; DOI=10.1073/pnas.84.10.3264; RA Keller G.-A., Gould S., de Luca M., Subramani S.; RT "Firefly luciferase is targeted to peroxisomes in mammalian cells."; RL Proc. Natl. Acad. Sci. U.S.A. 84:3264-3268(1987). RN [4] RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS). RX PubMed=8805533; DOI=10.1016/s0969-2126(96)00033-0; RA Conti E., Franks N.P., Brick P.; RT "Crystal structure of firefly luciferase throws light on a superfamily of RT adenylate-forming enzymes."; RL Structure 4:287-298(1996). RN [5] RP X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS). RX PubMed=9788915; DOI=10.1016/s0006-3495(98)77664-7; RA Franks N.P., Jenkins A., Conti E., Lieb W.R., Brick P.; RT "Structural basis for the inhibition of firefly luciferase by a general RT anesthetic."; RL Biophys. J. 75:2205-2211(1998). CC -!- FUNCTION: Produces green light with a wavelength of 562 nm. CC {ECO:0000250|UniProtKB:Q26304}. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + firefly D-luciferin + O2 = AMP + CO2 + diphosphate + CC firefly oxyluciferin + hnu; Xref=Rhea:RHEA:10732, ChEBI:CHEBI:15379, CC ChEBI:CHEBI:16526, ChEBI:CHEBI:16792, ChEBI:CHEBI:30212, CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58038, CC ChEBI:CHEBI:456215; EC=1.13.12.7; CC Evidence={ECO:0000250|UniProtKB:Q26304}; CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; CC -!- SUBCELLULAR LOCATION: Peroxisome {ECO:0000269|PubMed:3554235}. CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family. CC {ECO:0000305}. CC -!- WEB RESOURCE: Name=Wikipedia; Note=Firefly luciferase entry; CC URL="https://en.wikipedia.org/wiki/Firefly_luciferase"; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M15077; AAA29795.1; -; Genomic_DNA. DR EMBL; X84846; CAA59281.1; -; Genomic_DNA. DR EMBL; X84848; CAA59283.1; -; Genomic_DNA. DR EMBL; U03687; AAA03561.1; -; Unassigned_DNA. DR EMBL; U89934; AAB64396.1; -; Genomic_DNA. DR EMBL; U89935; AAB64399.1; -; Genomic_DNA. DR PIR; A26772; A26772. DR PDB; 1BA3; X-ray; 2.20 A; A=1-550. DR PDB; 1LCI; X-ray; 2.00 A; A=1-550. DR PDB; 3IEP; X-ray; 2.10 A; A=1-550. DR PDB; 3IER; X-ray; 2.05 A; A=1-550. DR PDB; 3IES; X-ray; 2.00 A; A=1-550. DR PDB; 3RIX; X-ray; 1.70 A; A=1-550. DR PDB; 4E5D; X-ray; 2.20 A; A=1-550. DR PDB; 4G36; X-ray; 2.62 A; A/B=1-550. DR PDB; 4G37; X-ray; 2.40 A; A/B=1-550. DR PDB; 5DV9; X-ray; 2.40 A; A=1-550. DR PDB; 5DWV; X-ray; 2.30 A; A=1-550. DR PDB; 5GYZ; X-ray; 2.40 A; A=4-438. DR PDB; 5GZ2; X-ray; 2.00 A; A=3-438. DR PDB; 5KYT; X-ray; 2.00 A; A/B=1-550. DR PDB; 5KYV; X-ray; 2.50 A; A/B=1-550. DR PDB; 5WYS; X-ray; 3.00 A; A=1-550. DR PDB; 6HPS; X-ray; 3.10 A; A/B=4-546. DR PDB; 6Q2M; X-ray; 2.75 A; A/B/C=1-550. DR PDBsum; 1BA3; -. DR PDBsum; 1LCI; -. DR PDBsum; 3IEP; -. DR PDBsum; 3IER; -. DR PDBsum; 3IES; -. DR PDBsum; 3RIX; -. DR PDBsum; 4E5D; -. DR PDBsum; 4G36; -. DR PDBsum; 4G37; -. DR PDBsum; 5DV9; -. DR PDBsum; 5DWV; -. DR PDBsum; 5GYZ; -. DR PDBsum; 5GZ2; -. DR PDBsum; 5KYT; -. DR PDBsum; 5KYV; -. DR PDBsum; 5WYS; -. DR PDBsum; 6HPS; -. DR PDBsum; 6Q2M; -. DR AlphaFoldDB; P08659; -. DR BMRB; P08659; -. DR SMR; P08659; -. DR ELM; P08659; -. DR IntAct; P08659; 1. DR MINT; P08659; -. DR BindingDB; P08659; -. DR ChEMBL; CHEMBL5567; -. DR OrthoDB; 3185532at2759; -. DR BRENDA; 1.13.12.7; 4775. DR BRENDA; 6.2.1.3; 4775. DR SABIO-RK; P08659; -. DR EvolutionaryTrace; P08659; -. DR GO; GO:0005777; C:peroxisome; IDA:UniProtKB. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0047077; F:Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing) activity; IEA:UniProtKB-EC. DR GO; GO:0051087; F:protein-folding chaperone binding; IPI:CAFA. DR GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW. DR CDD; cd17642; Firefly_Luc; 1. DR Gene3D; 3.30.300.30; -; 1. DR Gene3D; 3.40.50.980; -; 2. DR InterPro; IPR025110; AMP-bd_C. DR InterPro; IPR045851; AMP-bd_C_sf. DR InterPro; IPR020845; AMP-binding_CS. DR InterPro; IPR000873; AMP-dep_Synth/Lig_com. DR PANTHER; PTHR24096:SF353; GH16244P-RELATED; 1. DR PANTHER; PTHR24096; LONG-CHAIN-FATTY-ACID--COA LIGASE; 1. DR Pfam; PF00501; AMP-binding; 1. DR Pfam; PF13193; AMP-binding_C; 1. DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1. DR PROSITE; PS00455; AMP_BINDING; 1. PE 1: Evidence at protein level; KW 3D-structure; ATP-binding; Luminescence; Magnesium; Metal-binding; KW Monooxygenase; Nucleotide-binding; Oxidoreductase; Peroxisome; KW Photoprotein. FT CHAIN 1..550 FT /note="Luciferin 4-monooxygenase" FT /id="PRO_0000193147" FT MOTIF 548..550 FT /note="Microbody targeting signal" FT CONFLICT 361 FT /note="A -> G (in Ref. 2; CAA59281)" FT /evidence="ECO:0000305" FT HELIX 4..6 FT /evidence="ECO:0007829|PDB:3RIX" FT STRAND 7..9 FT /evidence="ECO:0007829|PDB:3RIX" FT HELIX 22..34 FT /evidence="ECO:0007829|PDB:3RIX" FT STRAND 40..44 FT /evidence="ECO:0007829|PDB:3RIX" FT TURN 45..47 FT /evidence="ECO:0007829|PDB:3RIX" FT STRAND 50..52 FT /evidence="ECO:0007829|PDB:3RIX" FT HELIX 53..70 FT /evidence="ECO:0007829|PDB:3RIX" FT STRAND 77..81 FT /evidence="ECO:0007829|PDB:3RIX" FT TURN 86..88 FT /evidence="ECO:0007829|PDB:3RIX" FT HELIX 89..97 FT /evidence="ECO:0007829|PDB:3RIX" FT STRAND 101..104 FT /evidence="ECO:0007829|PDB:3RIX" FT HELIX 111..121 FT /evidence="ECO:0007829|PDB:3RIX" FT STRAND 124..128 FT /evidence="ECO:0007829|PDB:3RIX" FT HELIX 130..132 FT /evidence="ECO:0007829|PDB:3RIX" FT HELIX 133..142 FT /evidence="ECO:0007829|PDB:3RIX" FT STRAND 148..151 FT /evidence="ECO:0007829|PDB:3RIX" FT STRAND 161..163 FT /evidence="ECO:0007829|PDB:1LCI" FT HELIX 164..171 FT /evidence="ECO:0007829|PDB:3RIX" FT TURN 178..180 FT /evidence="ECO:0007829|PDB:3RIX" FT TURN 188..190 FT /evidence="ECO:0007829|PDB:3RIX" FT STRAND 191..197 FT /evidence="ECO:0007829|PDB:3RIX" FT STRAND 201..204 FT /evidence="ECO:0007829|PDB:5KYT" FT STRAND 207..211 FT /evidence="ECO:0007829|PDB:3RIX" FT HELIX 212..222 FT /evidence="ECO:0007829|PDB:3RIX" FT TURN 225..227 FT /evidence="ECO:0007829|PDB:3RIX" FT STRAND 236..239 FT /evidence="ECO:0007829|PDB:3RIX" FT HELIX 246..258 FT /evidence="ECO:0007829|PDB:3RIX" FT STRAND 261..264 FT /evidence="ECO:0007829|PDB:3RIX" FT HELIX 270..279 FT /evidence="ECO:0007829|PDB:3RIX" FT STRAND 283..287 FT /evidence="ECO:0007829|PDB:3RIX" FT HELIX 289..297 FT /evidence="ECO:0007829|PDB:3RIX" FT HELIX 300..303 FT /evidence="ECO:0007829|PDB:3RIX" FT STRAND 311..314 FT /evidence="ECO:0007829|PDB:3RIX" FT HELIX 321..330 FT /evidence="ECO:0007829|PDB:3RIX" FT STRAND 337..341 FT /evidence="ECO:0007829|PDB:3RIX" FT HELIX 343..345 FT /evidence="ECO:0007829|PDB:3RIX" FT STRAND 346..351 FT /evidence="ECO:0007829|PDB:3RIX" FT STRAND 363..365 FT /evidence="ECO:0007829|PDB:3RIX" FT STRAND 370..374 FT /evidence="ECO:0007829|PDB:3RIX" FT TURN 376..378 FT /evidence="ECO:0007829|PDB:3RIX" FT STRAND 388..394 FT /evidence="ECO:0007829|PDB:3RIX" FT STRAND 399..401 FT /evidence="ECO:0007829|PDB:3RIX" FT HELIX 405..411 FT /evidence="ECO:0007829|PDB:3RIX" FT TURN 414..416 FT /evidence="ECO:0007829|PDB:5WYS" FT STRAND 418..426 FT /evidence="ECO:0007829|PDB:3RIX" FT STRAND 432..434 FT /evidence="ECO:0007829|PDB:3RIX" FT HELIX 438..440 FT /evidence="ECO:0007829|PDB:5KYT" FT STRAND 442..444 FT /evidence="ECO:0007829|PDB:1LCI" FT STRAND 447..449 FT /evidence="ECO:0007829|PDB:1LCI" FT HELIX 451..459 FT /evidence="ECO:0007829|PDB:1LCI" FT STRAND 464..474 FT /evidence="ECO:0007829|PDB:1LCI" FT TURN 475..477 FT /evidence="ECO:0007829|PDB:1LCI" FT STRAND 478..487 FT /evidence="ECO:0007829|PDB:1LCI" FT HELIX 495..505 FT /evidence="ECO:0007829|PDB:1LCI" FT HELIX 508..510 FT /evidence="ECO:0007829|PDB:1LCI" FT STRAND 515..521 FT /evidence="ECO:0007829|PDB:1LCI" FT STRAND 528..530 FT /evidence="ECO:0007829|PDB:1BA3" FT HELIX 532..542 FT /evidence="ECO:0007829|PDB:1LCI" FT TURN 544..546 FT /evidence="ECO:0007829|PDB:4G36" SQ SEQUENCE 550 AA; 60745 MW; E380FCE9D56ACCDE CRC64; MEDAKNIKKG PAPFYPLEDG TAGEQLHKAM KRYALVPGTI AFTDAHIEVN ITYAEYFEMS VRLAEAMKRY GLNTNHRIVV CSENSLQFFM PVLGALFIGV AVAPANDIYN ERELLNSMNI SQPTVVFVSK KGLQKILNVQ KKLPIIQKII IMDSKTDYQG FQSMYTFVTS HLPPGFNEYD FVPESFDRDK TIALIMNSSG STGLPKGVAL PHRTACVRFS HARDPIFGNQ IIPDTAILSV VPFHHGFGMF TTLGYLICGF RVVLMYRFEE ELFLRSLQDY KIQSALLVPT LFSFFAKSTL IDKYDLSNLH EIASGGAPLS KEVGEAVAKR FHLPGIRQGY GLTETTSAIL ITPEGDDKPG AVGKVVPFFE AKVVDLDTGK TLGVNQRGEL CVRGPMIMSG YVNNPEATNA LIDKDGWLHS GDIAYWDEDE HFFIVDRLKS LIKYKGYQVA PAELESILLQ HPNIFDAGVA GLPDDDAGEL PAAVVVLEHG KTMTEKEIVD YVASQVTTAK KLRGGVVFVD EVPKGLTGKL DARKIREILI KAKKGGKSKL //