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P08659

- LUCI_PHOPY

UniProt

P08659 - LUCI_PHOPY

Protein

Luciferin 4-monooxygenase

Gene
N/A
Organism
Photinus pyralis (Common eastern firefly) (Lampyris pyralis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Jan 1988)
      Previous versions | rss
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    Functioni

    Produces green light with a wavelength of 562 nm.

    Catalytic activityi

    Photinus luciferin + O2 + ATP = oxidized Photinus luciferin + CO2 + AMP + diphosphate + light.

    Cofactori

    Magnesium.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing) activity Source: UniProtKB-EC

    GO - Biological processi

    1. bioluminescence Source: UniProtKB-KW

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase, Photoprotein

    Keywords - Biological processi

    Luminescence

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16917.
    SABIO-RKP08659.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Luciferin 4-monooxygenase (EC:1.13.12.7)
    Short name:
    Luciferase
    OrganismiPhotinus pyralis (Common eastern firefly) (Lampyris pyralis)
    Taxonomic identifieri7054 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaColeopteraPolyphagaElateriformiaElateroideaLampyridaeLampyrinaePhotinus

    Subcellular locationi

    Peroxisome 1 Publication

    GO - Cellular componenti

    1. peroxisome Source: UniProtKB

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 550550Luciferin 4-monooxygenasePRO_0000193147Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    IntActiP08659. 1 interaction.
    MINTiMINT-7890251.

    Structurei

    Secondary structure

    1
    550
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 63
    Beta strandi7 – 93
    Helixi22 – 3413
    Beta strandi40 – 445
    Turni45 – 473
    Beta strandi50 – 523
    Helixi53 – 7018
    Beta strandi77 – 815
    Turni86 – 883
    Helixi89 – 979
    Beta strandi101 – 1044
    Helixi111 – 12111
    Beta strandi124 – 1285
    Helixi130 – 1323
    Helixi133 – 14210
    Beta strandi148 – 1514
    Beta strandi161 – 1633
    Helixi164 – 1718
    Turni178 – 1803
    Turni188 – 1903
    Beta strandi191 – 1977
    Beta strandi207 – 2115
    Helixi212 – 22211
    Turni225 – 2273
    Beta strandi236 – 2394
    Helixi246 – 25813
    Beta strandi261 – 2644
    Helixi270 – 27910
    Beta strandi283 – 2875
    Helixi289 – 2979
    Helixi300 – 3034
    Beta strandi311 – 3144
    Helixi321 – 33010
    Beta strandi337 – 3415
    Helixi343 – 3453
    Beta strandi346 – 3516
    Beta strandi363 – 3653
    Beta strandi370 – 3745
    Turni376 – 3783
    Beta strandi388 – 3947
    Beta strandi399 – 4013
    Helixi405 – 4117
    Beta strandi418 – 4269
    Beta strandi432 – 4343
    Helixi437 – 4393
    Beta strandi442 – 4443
    Beta strandi447 – 4493
    Helixi451 – 4599
    Beta strandi464 – 47411
    Turni475 – 4773
    Beta strandi478 – 48710
    Helixi495 – 50511
    Helixi508 – 5103
    Beta strandi515 – 5217
    Beta strandi528 – 5303
    Helixi532 – 54211

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BA3X-ray2.20A1-550[»]
    1LCIX-ray2.00A1-550[»]
    3IEPX-ray2.10A1-550[»]
    3IERX-ray2.05A1-550[»]
    3IESX-ray2.00A1-550[»]
    3RIXX-ray1.70A1-550[»]
    4E5DX-ray2.20A1-550[»]
    4G36X-ray2.62A/B1-550[»]
    4G37X-ray2.40A/B1-550[»]
    ProteinModelPortaliP08659.
    SMRiP08659. Positions 3-544.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP08659.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi548 – 5503Microbody targeting signal

    Sequence similaritiesi

    Family and domain databases

    Gene3Di4.10.8.10. 1 hit.
    InterProiIPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    IPR027380. Luciferase_N_dom.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view]
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P08659-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDAKNIKKG PAPFYPLEDG TAGEQLHKAM KRYALVPGTI AFTDAHIEVN    50
    ITYAEYFEMS VRLAEAMKRY GLNTNHRIVV CSENSLQFFM PVLGALFIGV 100
    AVAPANDIYN ERELLNSMNI SQPTVVFVSK KGLQKILNVQ KKLPIIQKII 150
    IMDSKTDYQG FQSMYTFVTS HLPPGFNEYD FVPESFDRDK TIALIMNSSG 200
    STGLPKGVAL PHRTACVRFS HARDPIFGNQ IIPDTAILSV VPFHHGFGMF 250
    TTLGYLICGF RVVLMYRFEE ELFLRSLQDY KIQSALLVPT LFSFFAKSTL 300
    IDKYDLSNLH EIASGGAPLS KEVGEAVAKR FHLPGIRQGY GLTETTSAIL 350
    ITPEGDDKPG AVGKVVPFFE AKVVDLDTGK TLGVNQRGEL CVRGPMIMSG 400
    YVNNPEATNA LIDKDGWLHS GDIAYWDEDE HFFIVDRLKS LIKYKGYQVA 450
    PAELESILLQ HPNIFDAGVA GLPDDDAGEL PAAVVVLEHG KTMTEKEIVD 500
    YVASQVTTAK KLRGGVVFVD EVPKGLTGKL DARKIREILI KAKKGGKSKL 550
    Length:550
    Mass (Da):60,745
    Last modified:January 1, 1988 - v1
    Checksum:iE380FCE9D56ACCDE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti361 – 3611A → G in CAA59281. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15077 Genomic DNA. Translation: AAA29795.1.
    X84846 Genomic DNA. Translation: CAA59281.1.
    X84848 Genomic DNA. Translation: CAA59283.1.
    U03687 Unassigned DNA. Translation: AAA03561.1.
    U89934 Genomic DNA. Translation: AAB64396.1.
    U89935 Genomic DNA. Translation: AAB64399.1.
    PIRiA26772.

    Cross-referencesi

    Web resourcesi

    Wikipedia

    Firefly luciferase entry

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15077 Genomic DNA. Translation: AAA29795.1 .
    X84846 Genomic DNA. Translation: CAA59281.1 .
    X84848 Genomic DNA. Translation: CAA59283.1 .
    U03687 Unassigned DNA. Translation: AAA03561.1 .
    U89934 Genomic DNA. Translation: AAB64396.1 .
    U89935 Genomic DNA. Translation: AAB64399.1 .
    PIRi A26772.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BA3 X-ray 2.20 A 1-550 [» ]
    1LCI X-ray 2.00 A 1-550 [» ]
    3IEP X-ray 2.10 A 1-550 [» ]
    3IER X-ray 2.05 A 1-550 [» ]
    3IES X-ray 2.00 A 1-550 [» ]
    3RIX X-ray 1.70 A 1-550 [» ]
    4E5D X-ray 2.20 A 1-550 [» ]
    4G36 X-ray 2.62 A/B 1-550 [» ]
    4G37 X-ray 2.40 A/B 1-550 [» ]
    ProteinModelPortali P08659.
    SMRi P08659. Positions 3-544.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P08659. 1 interaction.
    MINTi MINT-7890251.

    Chemistry

    BindingDBi P08659.
    ChEMBLi CHEMBL5567.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-16917.
    SABIO-RK P08659.

    Miscellaneous databases

    EvolutionaryTracei P08659.

    Family and domain databases

    Gene3Di 4.10.8.10. 1 hit.
    InterProi IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    IPR027380. Luciferase_N_dom.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view ]
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Firefly luciferase gene: structure and expression in mammalian cells."
      de Wet J.R., Wood K.V., Deluca M., Helinski D.R., Subramani S.
      Mol. Cell. Biol. 7:725-737(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Construction and utilization of an Autographa californica nuclear polyhedrosis virus vector with a unique cloning site: expression of genes amplified by the polymerase chain reaction."
      Croizier G.M.J.
      Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Firefly luciferase is targeted to peroxisomes in mammalian cells."
      Keller G.-A., Gould S., de Luca M., Subramani S.
      Proc. Natl. Acad. Sci. U.S.A. 84:3264-3268(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    4. "Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes."
      Conti E., Franks N.P., Brick P.
      Structure 4:287-298(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
    5. "Structural basis for the inhibition of firefly luciferase by a general anesthetic."
      Franks N.P., Jenkins A., Conti E., Lieb W.R., Brick P.
      Biophys. J. 75:2205-2211(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).

    Entry informationi

    Entry nameiLUCI_PHOPY
    AccessioniPrimary (citable) accession number: P08659
    Secondary accession number(s): Q27755
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 1, 1988
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3