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P08659

- LUCI_PHOPY

UniProt

P08659 - LUCI_PHOPY

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Protein
Luciferin 4-monooxygenase
Gene
N/A
Organism
Photinus pyralis (Common eastern firefly) (Lampyris pyralis)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Produces green light with a wavelength of 562 nm.

Catalytic activityi

Photinus luciferin + O2 + ATP = oxidized Photinus luciferin + CO2 + AMP + diphosphate + light.

Cofactori

Magnesium.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing) activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. bioluminescence Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase, Photoprotein

Keywords - Biological processi

Luminescence

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16917.
SABIO-RKP08659.

Names & Taxonomyi

Protein namesi
Recommended name:
Luciferin 4-monooxygenase (EC:1.13.12.7)
Short name:
Luciferase
OrganismiPhotinus pyralis (Common eastern firefly) (Lampyris pyralis)
Taxonomic identifieri7054 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaColeopteraPolyphagaElateriformiaElateroideaLampyridaeLampyrinaePhotinus

Subcellular locationi

Peroxisome 1 Publication

GO - Cellular componenti

  1. peroxisome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 550550Luciferin 4-monooxygenase
PRO_0000193147Add
BLAST

Interactioni

Protein-protein interaction databases

IntActiP08659. 1 interaction.
MINTiMINT-7890251.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 63
Beta strandi7 – 93
Helixi22 – 3413
Beta strandi40 – 445
Turni45 – 473
Beta strandi50 – 523
Helixi53 – 7018
Beta strandi77 – 815
Turni86 – 883
Helixi89 – 979
Beta strandi101 – 1044
Helixi111 – 12111
Beta strandi124 – 1285
Helixi130 – 1323
Helixi133 – 14210
Beta strandi148 – 1514
Beta strandi161 – 1633
Helixi164 – 1718
Turni178 – 1803
Turni188 – 1903
Beta strandi191 – 1977
Beta strandi207 – 2115
Helixi212 – 22211
Turni225 – 2273
Beta strandi236 – 2394
Helixi246 – 25813
Beta strandi261 – 2644
Helixi270 – 27910
Beta strandi283 – 2875
Helixi289 – 2979
Helixi300 – 3034
Beta strandi311 – 3144
Helixi321 – 33010
Beta strandi337 – 3415
Helixi343 – 3453
Beta strandi346 – 3516
Beta strandi363 – 3653
Beta strandi370 – 3745
Turni376 – 3783
Beta strandi388 – 3947
Beta strandi399 – 4013
Helixi405 – 4117
Beta strandi418 – 4269
Beta strandi432 – 4343
Helixi437 – 4393
Beta strandi442 – 4443
Beta strandi447 – 4493
Helixi451 – 4599
Beta strandi464 – 47411
Turni475 – 4773
Beta strandi478 – 48710
Helixi495 – 50511
Helixi508 – 5103
Beta strandi515 – 5217
Beta strandi528 – 5303
Helixi532 – 54211

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BA3X-ray2.20A1-550[»]
1LCIX-ray2.00A1-550[»]
3IEPX-ray2.10A1-550[»]
3IERX-ray2.05A1-550[»]
3IESX-ray2.00A1-550[»]
3RIXX-ray1.70A1-550[»]
4E5DX-ray2.20A1-550[»]
4G36X-ray2.62A/B1-550[»]
4G37X-ray2.40A/B1-550[»]
ProteinModelPortaliP08659.
SMRiP08659. Positions 3-544.

Miscellaneous databases

EvolutionaryTraceiP08659.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi548 – 5503Microbody targeting signal

Sequence similaritiesi

Family and domain databases

Gene3Di4.10.8.10. 1 hit.
InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR027380. Luciferase_N_dom.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08659-1 [UniParc]FASTAAdd to Basket

« Hide

MEDAKNIKKG PAPFYPLEDG TAGEQLHKAM KRYALVPGTI AFTDAHIEVN    50
ITYAEYFEMS VRLAEAMKRY GLNTNHRIVV CSENSLQFFM PVLGALFIGV 100
AVAPANDIYN ERELLNSMNI SQPTVVFVSK KGLQKILNVQ KKLPIIQKII 150
IMDSKTDYQG FQSMYTFVTS HLPPGFNEYD FVPESFDRDK TIALIMNSSG 200
STGLPKGVAL PHRTACVRFS HARDPIFGNQ IIPDTAILSV VPFHHGFGMF 250
TTLGYLICGF RVVLMYRFEE ELFLRSLQDY KIQSALLVPT LFSFFAKSTL 300
IDKYDLSNLH EIASGGAPLS KEVGEAVAKR FHLPGIRQGY GLTETTSAIL 350
ITPEGDDKPG AVGKVVPFFE AKVVDLDTGK TLGVNQRGEL CVRGPMIMSG 400
YVNNPEATNA LIDKDGWLHS GDIAYWDEDE HFFIVDRLKS LIKYKGYQVA 450
PAELESILLQ HPNIFDAGVA GLPDDDAGEL PAAVVVLEHG KTMTEKEIVD 500
YVASQVTTAK KLRGGVVFVD EVPKGLTGKL DARKIREILI KAKKGGKSKL 550
Length:550
Mass (Da):60,745
Last modified:January 1, 1988 - v1
Checksum:iE380FCE9D56ACCDE
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti361 – 3611A → G in CAA59281. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M15077 Genomic DNA. Translation: AAA29795.1.
X84846 Genomic DNA. Translation: CAA59281.1.
X84848 Genomic DNA. Translation: CAA59283.1.
U03687 Unassigned DNA. Translation: AAA03561.1.
U89934 Genomic DNA. Translation: AAB64396.1.
U89935 Genomic DNA. Translation: AAB64399.1.
PIRiA26772.

Cross-referencesi

Web resourcesi

Wikipedia

Firefly luciferase entry

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M15077 Genomic DNA. Translation: AAA29795.1 .
X84846 Genomic DNA. Translation: CAA59281.1 .
X84848 Genomic DNA. Translation: CAA59283.1 .
U03687 Unassigned DNA. Translation: AAA03561.1 .
U89934 Genomic DNA. Translation: AAB64396.1 .
U89935 Genomic DNA. Translation: AAB64399.1 .
PIRi A26772.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1BA3 X-ray 2.20 A 1-550 [» ]
1LCI X-ray 2.00 A 1-550 [» ]
3IEP X-ray 2.10 A 1-550 [» ]
3IER X-ray 2.05 A 1-550 [» ]
3IES X-ray 2.00 A 1-550 [» ]
3RIX X-ray 1.70 A 1-550 [» ]
4E5D X-ray 2.20 A 1-550 [» ]
4G36 X-ray 2.62 A/B 1-550 [» ]
4G37 X-ray 2.40 A/B 1-550 [» ]
ProteinModelPortali P08659.
SMRi P08659. Positions 3-544.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P08659. 1 interaction.
MINTi MINT-7890251.

Chemistry

BindingDBi P08659.
ChEMBLi CHEMBL5567.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-16917.
SABIO-RK P08659.

Miscellaneous databases

EvolutionaryTracei P08659.

Family and domain databases

Gene3Di 4.10.8.10. 1 hit.
InterProi IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR027380. Luciferase_N_dom.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Firefly luciferase gene: structure and expression in mammalian cells."
    de Wet J.R., Wood K.V., Deluca M., Helinski D.R., Subramani S.
    Mol. Cell. Biol. 7:725-737(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Construction and utilization of an Autographa californica nuclear polyhedrosis virus vector with a unique cloning site: expression of genes amplified by the polymerase chain reaction."
    Croizier G.M.J.
    Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Firefly luciferase is targeted to peroxisomes in mammalian cells."
    Keller G.-A., Gould S., de Luca M., Subramani S.
    Proc. Natl. Acad. Sci. U.S.A. 84:3264-3268(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  4. "Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes."
    Conti E., Franks N.P., Brick P.
    Structure 4:287-298(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
  5. "Structural basis for the inhibition of firefly luciferase by a general anesthetic."
    Franks N.P., Jenkins A., Conti E., Lieb W.R., Brick P.
    Biophys. J. 75:2205-2211(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).

Entry informationi

Entry nameiLUCI_PHOPY
AccessioniPrimary (citable) accession number: P08659
Secondary accession number(s): Q27755
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: December 11, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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