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Protein

Luciferin 4-monooxygenase

Gene
N/A
Organism
Photinus pyralis (Common eastern firefly) (Lampyris pyralis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Produces green light with a wavelength of 562 nm.

Catalytic activityi

Photinus luciferin + O2 + ATP = oxidized Photinus luciferin + CO2 + AMP + diphosphate + light.

Cofactori

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase, Photoprotein

Keywords - Biological processi

Luminescence

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16917.
BRENDAi1.13.12.7. 4775.
6.2.1.3. 4775.
SABIO-RKP08659.

Names & Taxonomyi

Protein namesi
Recommended name:
Luciferin 4-monooxygenase (EC:1.13.12.7)
Short name:
Luciferase
OrganismiPhotinus pyralis (Common eastern firefly) (Lampyris pyralis)
Taxonomic identifieri7054 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaColeopteraPolyphagaElateriformiaElateroideaLampyridaeLampyrinaePhotinus

Subcellular locationi

GO - Cellular componenti

  • peroxisome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5567.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001931471 – 550Luciferin 4-monooxygenaseAdd BLAST550

Interactioni

Protein-protein interaction databases

IntActiP08659. 1 interactor.
MINTiMINT-7890251.

Chemistry databases

BindingDBiP08659.

Structurei

Secondary structure

1550
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 6Combined sources3
Beta strandi7 – 9Combined sources3
Helixi22 – 34Combined sources13
Beta strandi40 – 44Combined sources5
Turni45 – 47Combined sources3
Beta strandi50 – 52Combined sources3
Helixi53 – 70Combined sources18
Beta strandi77 – 81Combined sources5
Turni86 – 88Combined sources3
Helixi89 – 97Combined sources9
Beta strandi101 – 104Combined sources4
Helixi111 – 121Combined sources11
Beta strandi124 – 128Combined sources5
Helixi130 – 132Combined sources3
Helixi133 – 142Combined sources10
Beta strandi148 – 151Combined sources4
Beta strandi161 – 163Combined sources3
Helixi164 – 171Combined sources8
Turni178 – 180Combined sources3
Turni188 – 190Combined sources3
Beta strandi191 – 197Combined sources7
Beta strandi207 – 211Combined sources5
Helixi212 – 222Combined sources11
Turni225 – 227Combined sources3
Beta strandi236 – 239Combined sources4
Helixi246 – 258Combined sources13
Beta strandi261 – 264Combined sources4
Helixi270 – 279Combined sources10
Beta strandi283 – 287Combined sources5
Helixi289 – 297Combined sources9
Helixi300 – 303Combined sources4
Beta strandi311 – 314Combined sources4
Helixi321 – 330Combined sources10
Beta strandi337 – 341Combined sources5
Helixi343 – 345Combined sources3
Beta strandi346 – 351Combined sources6
Beta strandi363 – 365Combined sources3
Beta strandi370 – 374Combined sources5
Turni376 – 378Combined sources3
Beta strandi388 – 394Combined sources7
Beta strandi399 – 401Combined sources3
Helixi405 – 411Combined sources7
Beta strandi418 – 426Combined sources9
Beta strandi432 – 434Combined sources3
Helixi437 – 439Combined sources3
Beta strandi442 – 444Combined sources3
Beta strandi447 – 449Combined sources3
Helixi451 – 459Combined sources9
Beta strandi464 – 474Combined sources11
Turni475 – 477Combined sources3
Beta strandi478 – 487Combined sources10
Helixi495 – 505Combined sources11
Helixi508 – 510Combined sources3
Beta strandi515 – 521Combined sources7
Beta strandi528 – 530Combined sources3
Helixi532 – 542Combined sources11
Turni544 – 546Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BA3X-ray2.20A1-550[»]
1LCIX-ray2.00A1-550[»]
3IEPX-ray2.10A1-550[»]
3IERX-ray2.05A1-550[»]
3IESX-ray2.00A1-550[»]
3RIXX-ray1.70A1-550[»]
4E5DX-ray2.20A1-550[»]
4G36X-ray2.62A/B1-550[»]
4G37X-ray2.40A/B1-550[»]
5DV9X-ray2.40A1-550[»]
5DWVX-ray2.30A1-550[»]
ProteinModelPortaliP08659.
SMRiP08659.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08659.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi548 – 550Microbody targeting signal3

Sequence similaritiesi

Family and domain databases

Gene3Di4.10.8.10. 1 hit.
InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR027380. Luciferase_N_dom.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08659-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDAKNIKKG PAPFYPLEDG TAGEQLHKAM KRYALVPGTI AFTDAHIEVN
60 70 80 90 100
ITYAEYFEMS VRLAEAMKRY GLNTNHRIVV CSENSLQFFM PVLGALFIGV
110 120 130 140 150
AVAPANDIYN ERELLNSMNI SQPTVVFVSK KGLQKILNVQ KKLPIIQKII
160 170 180 190 200
IMDSKTDYQG FQSMYTFVTS HLPPGFNEYD FVPESFDRDK TIALIMNSSG
210 220 230 240 250
STGLPKGVAL PHRTACVRFS HARDPIFGNQ IIPDTAILSV VPFHHGFGMF
260 270 280 290 300
TTLGYLICGF RVVLMYRFEE ELFLRSLQDY KIQSALLVPT LFSFFAKSTL
310 320 330 340 350
IDKYDLSNLH EIASGGAPLS KEVGEAVAKR FHLPGIRQGY GLTETTSAIL
360 370 380 390 400
ITPEGDDKPG AVGKVVPFFE AKVVDLDTGK TLGVNQRGEL CVRGPMIMSG
410 420 430 440 450
YVNNPEATNA LIDKDGWLHS GDIAYWDEDE HFFIVDRLKS LIKYKGYQVA
460 470 480 490 500
PAELESILLQ HPNIFDAGVA GLPDDDAGEL PAAVVVLEHG KTMTEKEIVD
510 520 530 540 550
YVASQVTTAK KLRGGVVFVD EVPKGLTGKL DARKIREILI KAKKGGKSKL
Length:550
Mass (Da):60,745
Last modified:January 1, 1988 - v1
Checksum:iE380FCE9D56ACCDE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti361A → G in CAA59281 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15077 Genomic DNA. Translation: AAA29795.1.
X84846 Genomic DNA. Translation: CAA59281.1.
X84848 Genomic DNA. Translation: CAA59283.1.
U03687 Unassigned DNA. Translation: AAA03561.1.
U89934 Genomic DNA. Translation: AAB64396.1.
U89935 Genomic DNA. Translation: AAB64399.1.
PIRiA26772.

Cross-referencesi

Web resourcesi

Wikipedia

Firefly luciferase entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15077 Genomic DNA. Translation: AAA29795.1.
X84846 Genomic DNA. Translation: CAA59281.1.
X84848 Genomic DNA. Translation: CAA59283.1.
U03687 Unassigned DNA. Translation: AAA03561.1.
U89934 Genomic DNA. Translation: AAB64396.1.
U89935 Genomic DNA. Translation: AAB64399.1.
PIRiA26772.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BA3X-ray2.20A1-550[»]
1LCIX-ray2.00A1-550[»]
3IEPX-ray2.10A1-550[»]
3IERX-ray2.05A1-550[»]
3IESX-ray2.00A1-550[»]
3RIXX-ray1.70A1-550[»]
4E5DX-ray2.20A1-550[»]
4G36X-ray2.62A/B1-550[»]
4G37X-ray2.40A/B1-550[»]
5DV9X-ray2.40A1-550[»]
5DWVX-ray2.30A1-550[»]
ProteinModelPortaliP08659.
SMRiP08659.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP08659. 1 interactor.
MINTiMINT-7890251.

Chemistry databases

BindingDBiP08659.
ChEMBLiCHEMBL5567.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16917.
BRENDAi1.13.12.7. 4775.
6.2.1.3. 4775.
SABIO-RKP08659.

Miscellaneous databases

EvolutionaryTraceiP08659.

Family and domain databases

Gene3Di4.10.8.10. 1 hit.
InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR027380. Luciferase_N_dom.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLUCI_PHOPY
AccessioniPrimary (citable) accession number: P08659
Secondary accession number(s): Q27755
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 30, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.