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P08659

- LUCI_PHOPY

UniProt

P08659 - LUCI_PHOPY

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Protein

Luciferin 4-monooxygenase

Gene
N/A
Organism
Photinus pyralis (Common eastern firefly) (Lampyris pyralis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Produces green light with a wavelength of 562 nm.

Catalytic activityi

Photinus luciferin + O2 + ATP = oxidized Photinus luciferin + CO2 + AMP + diphosphate + light.

Cofactori

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing) activity Source: UniProtKB-EC

GO - Biological processi

  1. bioluminescence Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase, Photoprotein

Keywords - Biological processi

Luminescence

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16917.
SABIO-RKP08659.

Names & Taxonomyi

Protein namesi
Recommended name:
Luciferin 4-monooxygenase (EC:1.13.12.7)
Short name:
Luciferase
OrganismiPhotinus pyralis (Common eastern firefly) (Lampyris pyralis)
Taxonomic identifieri7054 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaColeopteraPolyphagaElateriformiaElateroideaLampyridaeLampyrinaePhotinus

Subcellular locationi

Peroxisome 1 Publication

GO - Cellular componenti

  1. peroxisome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 550550Luciferin 4-monooxygenasePRO_0000193147Add
BLAST

Interactioni

Protein-protein interaction databases

IntActiP08659. 1 interaction.
MINTiMINT-7890251.

Structurei

Secondary structure

1
550
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 63Combined sources
Beta strandi7 – 93Combined sources
Helixi22 – 3413Combined sources
Beta strandi40 – 445Combined sources
Turni45 – 473Combined sources
Beta strandi50 – 523Combined sources
Helixi53 – 7018Combined sources
Beta strandi77 – 815Combined sources
Turni86 – 883Combined sources
Helixi89 – 979Combined sources
Beta strandi101 – 1044Combined sources
Helixi111 – 12111Combined sources
Beta strandi124 – 1285Combined sources
Helixi130 – 1323Combined sources
Helixi133 – 14210Combined sources
Beta strandi148 – 1514Combined sources
Beta strandi161 – 1633Combined sources
Helixi164 – 1718Combined sources
Turni178 – 1803Combined sources
Turni188 – 1903Combined sources
Beta strandi191 – 1977Combined sources
Beta strandi207 – 2115Combined sources
Helixi212 – 22211Combined sources
Turni225 – 2273Combined sources
Beta strandi236 – 2394Combined sources
Helixi246 – 25813Combined sources
Beta strandi261 – 2644Combined sources
Helixi270 – 27910Combined sources
Beta strandi283 – 2875Combined sources
Helixi289 – 2979Combined sources
Helixi300 – 3034Combined sources
Beta strandi311 – 3144Combined sources
Helixi321 – 33010Combined sources
Beta strandi337 – 3415Combined sources
Helixi343 – 3453Combined sources
Beta strandi346 – 3516Combined sources
Beta strandi363 – 3653Combined sources
Beta strandi370 – 3745Combined sources
Turni376 – 3783Combined sources
Beta strandi388 – 3947Combined sources
Beta strandi399 – 4013Combined sources
Helixi405 – 4117Combined sources
Beta strandi418 – 4269Combined sources
Beta strandi432 – 4343Combined sources
Helixi437 – 4393Combined sources
Beta strandi442 – 4443Combined sources
Beta strandi447 – 4493Combined sources
Helixi451 – 4599Combined sources
Beta strandi464 – 47411Combined sources
Turni475 – 4773Combined sources
Beta strandi478 – 48710Combined sources
Helixi495 – 50511Combined sources
Helixi508 – 5103Combined sources
Beta strandi515 – 5217Combined sources
Beta strandi528 – 5303Combined sources
Helixi532 – 54211Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BA3X-ray2.20A1-550[»]
1LCIX-ray2.00A1-550[»]
3IEPX-ray2.10A1-550[»]
3IERX-ray2.05A1-550[»]
3IESX-ray2.00A1-550[»]
3RIXX-ray1.70A1-550[»]
4E5DX-ray2.20A1-550[»]
4G36X-ray2.62A/B1-550[»]
4G37X-ray2.40A/B1-550[»]
ProteinModelPortaliP08659.
SMRiP08659. Positions 3-544.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08659.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi548 – 5503Microbody targeting signal

Sequence similaritiesi

Family and domain databases

Gene3Di4.10.8.10. 1 hit.
InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR027380. Luciferase_N_dom.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08659-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEDAKNIKKG PAPFYPLEDG TAGEQLHKAM KRYALVPGTI AFTDAHIEVN
60 70 80 90 100
ITYAEYFEMS VRLAEAMKRY GLNTNHRIVV CSENSLQFFM PVLGALFIGV
110 120 130 140 150
AVAPANDIYN ERELLNSMNI SQPTVVFVSK KGLQKILNVQ KKLPIIQKII
160 170 180 190 200
IMDSKTDYQG FQSMYTFVTS HLPPGFNEYD FVPESFDRDK TIALIMNSSG
210 220 230 240 250
STGLPKGVAL PHRTACVRFS HARDPIFGNQ IIPDTAILSV VPFHHGFGMF
260 270 280 290 300
TTLGYLICGF RVVLMYRFEE ELFLRSLQDY KIQSALLVPT LFSFFAKSTL
310 320 330 340 350
IDKYDLSNLH EIASGGAPLS KEVGEAVAKR FHLPGIRQGY GLTETTSAIL
360 370 380 390 400
ITPEGDDKPG AVGKVVPFFE AKVVDLDTGK TLGVNQRGEL CVRGPMIMSG
410 420 430 440 450
YVNNPEATNA LIDKDGWLHS GDIAYWDEDE HFFIVDRLKS LIKYKGYQVA
460 470 480 490 500
PAELESILLQ HPNIFDAGVA GLPDDDAGEL PAAVVVLEHG KTMTEKEIVD
510 520 530 540 550
YVASQVTTAK KLRGGVVFVD EVPKGLTGKL DARKIREILI KAKKGGKSKL
Length:550
Mass (Da):60,745
Last modified:January 1, 1988 - v1
Checksum:iE380FCE9D56ACCDE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti361 – 3611A → G in CAA59281. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15077 Genomic DNA. Translation: AAA29795.1.
X84846 Genomic DNA. Translation: CAA59281.1.
X84848 Genomic DNA. Translation: CAA59283.1.
U03687 Unassigned DNA. Translation: AAA03561.1.
U89934 Genomic DNA. Translation: AAB64396.1.
U89935 Genomic DNA. Translation: AAB64399.1.
PIRiA26772.

Cross-referencesi

Web resourcesi

Wikipedia

Firefly luciferase entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15077 Genomic DNA. Translation: AAA29795.1 .
X84846 Genomic DNA. Translation: CAA59281.1 .
X84848 Genomic DNA. Translation: CAA59283.1 .
U03687 Unassigned DNA. Translation: AAA03561.1 .
U89934 Genomic DNA. Translation: AAB64396.1 .
U89935 Genomic DNA. Translation: AAB64399.1 .
PIRi A26772.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1BA3 X-ray 2.20 A 1-550 [» ]
1LCI X-ray 2.00 A 1-550 [» ]
3IEP X-ray 2.10 A 1-550 [» ]
3IER X-ray 2.05 A 1-550 [» ]
3IES X-ray 2.00 A 1-550 [» ]
3RIX X-ray 1.70 A 1-550 [» ]
4E5D X-ray 2.20 A 1-550 [» ]
4G36 X-ray 2.62 A/B 1-550 [» ]
4G37 X-ray 2.40 A/B 1-550 [» ]
ProteinModelPortali P08659.
SMRi P08659. Positions 3-544.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P08659. 1 interaction.
MINTi MINT-7890251.

Chemistry

BindingDBi P08659.
ChEMBLi CHEMBL5567.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-16917.
SABIO-RK P08659.

Miscellaneous databases

EvolutionaryTracei P08659.

Family and domain databases

Gene3Di 4.10.8.10. 1 hit.
InterProi IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR027380. Luciferase_N_dom.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Firefly luciferase gene: structure and expression in mammalian cells."
    de Wet J.R., Wood K.V., Deluca M., Helinski D.R., Subramani S.
    Mol. Cell. Biol. 7:725-737(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Construction and utilization of an Autographa californica nuclear polyhedrosis virus vector with a unique cloning site: expression of genes amplified by the polymerase chain reaction."
    Croizier G.M.J.
    Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Firefly luciferase is targeted to peroxisomes in mammalian cells."
    Keller G.-A., Gould S., de Luca M., Subramani S.
    Proc. Natl. Acad. Sci. U.S.A. 84:3264-3268(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  4. "Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes."
    Conti E., Franks N.P., Brick P.
    Structure 4:287-298(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
  5. "Structural basis for the inhibition of firefly luciferase by a general anesthetic."
    Franks N.P., Jenkins A., Conti E., Lieb W.R., Brick P.
    Biophys. J. 75:2205-2211(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).

Entry informationi

Entry nameiLUCI_PHOPY
AccessioniPrimary (citable) accession number: P08659
Secondary accession number(s): Q27755
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 26, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3