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P08659 (LUCI_PHOPY) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Luciferin 4-monooxygenase

Short name=Luciferase
EC=1.13.12.7
OrganismPhotinus pyralis (Common eastern firefly) (Lampyris pyralis)
Taxonomic identifier7054 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaColeopteraPolyphagaElateriformiaElateroideaLampyridaeLampyrinaePhotinus

Protein attributes

Sequence length550 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Produces green light with a wavelength of 562 nm.

Catalytic activity

Photinus luciferin + O2 + ATP = oxidized Photinus luciferin + CO2 + AMP + diphosphate + light.

Cofactor

Magnesium.

Subcellular location

Peroxisome Ref.3.

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
   Biological processLuminescence
   Cellular componentPeroxisome
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionMonooxygenase
Oxidoreductase
Photoprotein
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processbioluminescence

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentperoxisome

Inferred from direct assay Ref.3. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing) activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 550550Luciferin 4-monooxygenase
PRO_0000193147

Regions

Motif548 – 5503Microbody targeting signal

Experimental info

Sequence conflict3611A → G in CAA59281. Ref.2

Secondary structure

...................................................................................................... 550
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P08659 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: E380FCE9D56ACCDE

FASTA55060,745
        10         20         30         40         50         60 
MEDAKNIKKG PAPFYPLEDG TAGEQLHKAM KRYALVPGTI AFTDAHIEVN ITYAEYFEMS 

        70         80         90        100        110        120 
VRLAEAMKRY GLNTNHRIVV CSENSLQFFM PVLGALFIGV AVAPANDIYN ERELLNSMNI 

       130        140        150        160        170        180 
SQPTVVFVSK KGLQKILNVQ KKLPIIQKII IMDSKTDYQG FQSMYTFVTS HLPPGFNEYD 

       190        200        210        220        230        240 
FVPESFDRDK TIALIMNSSG STGLPKGVAL PHRTACVRFS HARDPIFGNQ IIPDTAILSV 

       250        260        270        280        290        300 
VPFHHGFGMF TTLGYLICGF RVVLMYRFEE ELFLRSLQDY KIQSALLVPT LFSFFAKSTL 

       310        320        330        340        350        360 
IDKYDLSNLH EIASGGAPLS KEVGEAVAKR FHLPGIRQGY GLTETTSAIL ITPEGDDKPG 

       370        380        390        400        410        420 
AVGKVVPFFE AKVVDLDTGK TLGVNQRGEL CVRGPMIMSG YVNNPEATNA LIDKDGWLHS 

       430        440        450        460        470        480 
GDIAYWDEDE HFFIVDRLKS LIKYKGYQVA PAELESILLQ HPNIFDAGVA GLPDDDAGEL 

       490        500        510        520        530        540 
PAAVVVLEHG KTMTEKEIVD YVASQVTTAK KLRGGVVFVD EVPKGLTGKL DARKIREILI 

       550 
KAKKGGKSKL 

« Hide

References

[1]"Firefly luciferase gene: structure and expression in mammalian cells."
de Wet J.R., Wood K.V., Deluca M., Helinski D.R., Subramani S.
Mol. Cell. Biol. 7:725-737(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Construction and utilization of an Autographa californica nuclear polyhedrosis virus vector with a unique cloning site: expression of genes amplified by the polymerase chain reaction."
Croizier G.M.J.
Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Firefly luciferase is targeted to peroxisomes in mammalian cells."
Keller G.-A., Gould S., de Luca M., Subramani S.
Proc. Natl. Acad. Sci. U.S.A. 84:3264-3268(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[4]"Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes."
Conti E., Franks N.P., Brick P.
Structure 4:287-298(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[5]"Structural basis for the inhibition of firefly luciferase by a general anesthetic."
Franks N.P., Jenkins A., Conti E., Lieb W.R., Brick P.
Biophys. J. 75:2205-2211(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
+Additional computationally mapped references.

Web resources

Wikipedia

Firefly luciferase entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M15077 Genomic DNA. Translation: AAA29795.1.
X84846 Genomic DNA. Translation: CAA59281.1.
X84848 Genomic DNA. Translation: CAA59283.1.
U03687 Unassigned DNA. Translation: AAA03561.1.
U89934 Genomic DNA. Translation: AAB64396.1.
U89935 Genomic DNA. Translation: AAB64399.1.
PIRA26772.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1BA3X-ray2.20A1-550[»]
1LCIX-ray2.00A1-550[»]
3IEPX-ray2.10A1-550[»]
3IERX-ray2.05A1-550[»]
3IESX-ray2.00A1-550[»]
3RIXX-ray1.70A1-550[»]
4E5DX-ray2.20A1-550[»]
4G36X-ray2.62A/B1-550[»]
4G37X-ray2.40A/B1-550[»]
ProteinModelPortalP08659.
SMRP08659. Positions 3-544.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP08659. 1 interaction.
MINTMINT-7890251.

Chemistry

BindingDBP08659.
ChEMBLCHEMBL5567.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-16917.
SABIO-RKP08659.

Family and domain databases

Gene3D4.10.8.10. 1 hit.
InterProIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR027380. Luciferase_N_dom.
[Graphical view]
PfamPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP08659.

Entry information

Entry nameLUCI_PHOPY
AccessionPrimary (citable) accession number: P08659
Secondary accession number(s): Q27755
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: December 11, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references