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Protein

Lactose regulatory protein LAC9

Gene

LAC9

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Positive regulatory protein, that controls induction of the lactose-galactose regulation of Kluyveromyces lactis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi95 – 951Zinc 1
Metal bindingi95 – 951Zinc 2
Metal bindingi98 – 981Zinc 1
Metal bindingi105 – 1051Zinc 1
Metal bindingi112 – 1121Zinc 1
Metal bindingi112 – 1121Zinc 2
Metal bindingi115 – 1151Zinc 2
Metal bindingi122 – 1221Zinc 2

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi95 – 12228Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Carbohydrate metabolism, Galactose metabolism, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Lactose regulatory protein LAC9
Gene namesi
Name:LAC9
Ordered Locus Names:KLLA0D12672g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome D

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 865865Lactose regulatory protein LAC9PRO_0000114953Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi284590.XP_453627.1.

Structurei

Secondary structure

1
865
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi96 – 1005Combined sources
Beta strandi109 – 1113Combined sources
Helixi113 – 1186Combined sources
Helixi853 – 8597Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CLDNMR-A85-144[»]
3E1KX-ray3.00B/D/F/H/J/L/N/P844-865[»]
ProteinModelPortaliP08657.
SMRiP08657. Positions 93-125.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08657.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi68 – 8417His-richAdd
BLAST

Sequence similaritiesi

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IJFD. Eukaryota.
ENOG410YVCP. LUCA.
InParanoidiP08657.
KOiK09241.
OMAiFRYILWE.
OrthoDBiEOG7J44JV.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR005600. Gal4_dimer_dom.
IPR007219. Transcription_factor_dom_fun.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF04082. Fungal_trans. 1 hit.
PF03902. Gal4_dimer. 1 hit.
PF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00906. Fungal_trans. 1 hit.
SM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08657-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSRASNSPS FSSKAETLLP SEYKKNAVKK ETIRNGKKRK LPDTESSDPE
60 70 80 90 100
FASRRLIANE TGTDAVSNGN KNDSNANNNN NNNNKKSSEV MHQACDACRK
110 120 130 140 150
KKWKCSKTVP TCTNCLKYNL DCVYSPQVVR TPLTRAHLTE MENRVAELEQ
160 170 180 190 200
FLKELFPVWD IDRLLQQKDT YRIRELLTMG STNTVPGLAS NNIDSSLEQP
210 220 230 240 250
VAFGTAQPAQ SLSTDPAVQS QAYPMQPVPM TELQSITNLR HTPSLLDEQQ
260 270 280 290 300
MNTISTATLR NMYSSGNNNN NLGNISGLSP VTEAFFRWQE GETSIDNSYF
310 320 330 340 350
GKGSILFWLN QLLSSEKIAG VTSKVGNDIN TNNNNINHQK LPLILNNNIT
360 370 380 390 400
HNVSDITTTS TSSNKRAMSP LSANDSVYLA KRETISAYID AYFKHYHALY
410 420 430 440 450
PLVSKEMFFA QYNDQIKPEN VEIWHILLNA VLALGSWCSN SCSSHHTLYY
460 470 480 490 500
QNALSYLSTA VLETGSTDLT IALILLTHYV QKMHKPNTAW SLIGLCSHMA
510 520 530 540 550
TSLGLHRDLP NSTIHDQQLR RVLWWTIYCT GCDLSLETGR PSLLPNLQAI
560 570 580 590 600
DIPLPASSAT IKEPSIYSSI IQESQWSQIL QQKLSNNSYQ QSAGECLSWF
610 620 630 640 650
DSVQAFLDHW PTPSTEAELK ALNETQLDWL PLVKFRPYWM FHCSLISLFS
660 670 680 690 700
VFFEEDAPTD NNVIRCKELC LQLSSRNIFS VATFVRSYAF NSLSCWYATH
710 720 730 740 750
YLVRSALVPL HFASRISPQH ALWETVKAQL LSAHEAMGIL SQESSLAAKF
760 770 780 790 800
DGILTKNYSE ILQREGINKS QLMPPPTPLL QSTSFSDLLS LWSANAEDAP
810 820 830 840 850
RVSNSQMPQS ITITDSLLQS STTQMRPPTT SGWPDTNNFL NPSTQQLFNT
860
TTMDDVYNYI FDNDE
Length:865
Mass (Da):97,038
Last modified:January 1, 1988 - v1
Checksum:iD6CC912F238DBEBE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15210 Genomic DNA. Translation: AAA35266.1.
X06215 Genomic DNA. Translation: CAA29565.1.
CR382124 Genomic DNA. Translation: CAH00723.1.
PIRiA93641. A25762.
S08482.
RefSeqiXP_453627.1. XM_453627.1.

Genome annotation databases

EnsemblFungiiCAH00723; CAH00723; KLLA0_D12672g.
GeneIDi2893282.
KEGGikla:KLLA0D12672g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15210 Genomic DNA. Translation: AAA35266.1.
X06215 Genomic DNA. Translation: CAA29565.1.
CR382124 Genomic DNA. Translation: CAH00723.1.
PIRiA93641. A25762.
S08482.
RefSeqiXP_453627.1. XM_453627.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CLDNMR-A85-144[»]
3E1KX-ray3.00B/D/F/H/J/L/N/P844-865[»]
ProteinModelPortaliP08657.
SMRiP08657. Positions 93-125.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi284590.XP_453627.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAH00723; CAH00723; KLLA0_D12672g.
GeneIDi2893282.
KEGGikla:KLLA0D12672g.

Phylogenomic databases

eggNOGiENOG410IJFD. Eukaryota.
ENOG410YVCP. LUCA.
InParanoidiP08657.
KOiK09241.
OMAiFRYILWE.
OrthoDBiEOG7J44JV.

Miscellaneous databases

EvolutionaryTraceiP08657.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR005600. Gal4_dimer_dom.
IPR007219. Transcription_factor_dom_fun.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF04082. Fungal_trans. 1 hit.
PF03902. Gal4_dimer. 1 hit.
PF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00906. Fungal_trans. 1 hit.
SM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a positive regulatory gene, LAC9, that controls induction of the lactose-galactose regulon of Kluyveromyces lactis: structural and functional relationships to GAL4 of Saccharomyces cerevisiae."
    Wray L.V. Jr., Witte M.M., Dickson R.C., Riley M.I.
    Mol. Cell. Biol. 7:1111-1121(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of the Kluyveromyces lactis positive regulatory gene LAC9 reveals functional homology to, but sequence divergence from, the Saccharomyces cerevisiae GAL4 gene."
    Salmeron J.M., Johnston S.A.
    Nucleic Acids Res. 14:7767-7781(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.
  3. "Genome evolution in yeasts."
    Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
    , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
    Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.
  4. "A mutation in the Zn-finger of the GAL4 homolog LAC9 results in glucose repression of its target genes."
    Kuger P., Goedecke A., Breunig K.D.
    Nucleic Acids Res. 18:745-751(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.
  5. "Solution structure of the Kluyveromyces lactis LAC9 Cd2 Cys6 DNA-binding domain."
    Gardner K.H., Anderson S.F., Coleman J.E.
    Nat. Struct. Biol. 2:898-905(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 85-144.

Entry informationi

Entry nameiLAC9_KLULA
AccessioniPrimary (citable) accession number: P08657
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: June 8, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.