Nuclear factor 1 C-type - Homo sapiens (Human)

Names and origin

Protein names

Recommended name:
    Nuclear factor 1 C-type
      Short name=Nuclear factor 1/C
      Short name=NF1-C
Alternative name(s):
    Nuclear factor I/C
      Short name=NF-I/C
      Short name=NFI-C
    CCAAT-box-binding transcription factor
      Short name=CTF
    TGGCA-binding protein

Gene names

Name:	NFIC
Synonyms:	NFI

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Subunit structure	Binds DNA as a homodimer.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the CTF/NF-I family. Contains 1 CTF/NF-I DNA-binding domain.

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Ontologies

Keywords
Biological process	DNA replication Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of transcription from RNA polymerase II promoter Inferred from genetic interaction. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI transcription from RNA polymerase II promoter Ref.2 Traceable author statement. Source: ProtInc
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transcription factor activity Ref.2 Inferred from direct assay. Source: MGI
Complete GO annotation...

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Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 4 (identifier: P08651-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: P08651-2) The sequence of this isoform differs from the canonical sequence as follows: 1-10: MYSSPLCLTQ → M

Isoform 2 (identifier: P08651-3) The sequence of this isoform differs from the canonical sequence as follows: 1-10: MYSSPLCLTQ → M 424-439: LNGSGQLKMPSHCLSA → PTLRPTRPLQTVPLWD 440-508: Missing.

Isoform 3 (identifier: P08651-4) The sequence of this isoform differs from the canonical sequence as follows: 1-10: MYSSPLCLTQ → M 188-211: Missing. 424-439: LNGSGQLKMPSHCLSA → PTLRPTRPLQTVPLWD 440-508: Missing.

Isoform 5 (identifier: P08651-5) The sequence of this isoform differs from the canonical sequence as follows: 424-503: Missing.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 508

508

Nuclear factor 1 C-type

PRO_0000100199

Regions

DNA binding

1 – 195

195

CTF/NF-I

Amino acid modifications

Modified residue

4

1

Phosphoserine Ref.8

Modified residue

194

1

Phosphoserine Ref.8 Ref.7

Modified residue

294

1

Phosphoserine Ref.12

Modified residue

304

1

Phosphoserine Ref.10

Modified residue

305

1

Phosphoserine Ref.12 Ref.10

Modified residue

323

1

Phosphoserine Ref.8 Ref.12 Ref.9 Ref.11

Modified residue

333

1

Phosphoserine Ref.8 Ref.7 Ref.12 Ref.14

Modified residue

337

1

Phosphoserine By similarity

Modified residue

339

1

Phosphoserine Ref.8 Ref.7 Ref.12 Ref.10 Ref.9

Modified residue

343

1

Phosphoserine Ref.10

Modified residue

378

1

Phosphothreonine By similarity

Natural variations

Alternative sequence

1 – 10

10

MYSSPLCLTQ → M in isoform 1, isoform 2 and isoform 3.

VSP_003552

Alternative sequence

188 – 211

24

Missing in isoform 3.

VSP_003553

Alternative sequence

424 – 503

80

Missing in isoform 5.

VSP_003554

Alternative sequence

424 – 439

16

LNGSG…HCLSA → PTLRPTRPLQTVPLWD in isoform 2 and isoform 3.

VSP_003555

Alternative sequence

440 – 508

69

Missing in isoform 2 and isoform 3.

VSP_003556

Natural variant

417

1

A → S: dbSNP rs10412720.

VAR_057656

Experimental info

Sequence conflict

212

1

D → V in CAA63440. Ref.2

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Sequences

Sequence		Length	Mass (Da)
Isoform 4 [UniParc]. Last modified June 1, 2001. Version 2. Checksum: 3D0F97DA0F3AF992 Show »	FASTA	508	55,675
10 20 30 40 50 60 MYSSPLCLTQ DEFHPFIEAL LPHVRAFAYT WFNLQARKRK YFKKHEKRMS KDEERAVKDE 70 80 90 100 110 120 LLGEKPEVKQ KWASRLLAKL RKDIRPECRE DFVLSITGKK APGCVLSNPD QKGKMRRIDC 130 140 150 160 170 180 LRQADKVWRL DLVMVILFKG IPLESTDGER LVKAAQCGHP VLCVQPHHIG VAVKELDLYL 190 200 210 220 230 240 AYFVRERDAE QSGSPRTGMG SDQEDSKPIT LDTTDFQESF VTSGVFSVTE LIQVSRTPVV 250 260 270 280 290 300 TGTGPNFSLG ELQGHLAYDL NPASTGLRRT LPSTSSSGSK RHKSGSMEED VDTSPGGDYY 310 320 330 340 350 360 TSPSSPTSSS RNWTEDMEGG ISSPVKKTEM DKSPFNSPSP QDSPRLSSFT QHHRPVIAVH 370 380 390 400 410 420 SGIARSPHPS SALHFPTTSI LPQTASTYFP HTAIRYPPHL NPQDPLKDLV SLACDPASQQ 430 440 450 460 470 480 PGPLNGSGQL KMPSHCLSAQ MLAPPPPGLP RLALPPATKP ATTSEGGATS PTSPSYSPPD 490 500 TSPANRSFVG LGPRDPAGIY QAQSWYLG « Hide
Isoform 1. Checksum: B6A59BD10B4B6D1B Show »	FASTA	499	54,682
10 20 30 40 50 60 MDEFHPFIEA LLPHVRAFAY TWFNLQARKR KYFKKHEKRM SKDEERAVKD ELLGEKPEVK 70 80 90 100 110 120 QKWASRLLAK LRKDIRPECR EDFVLSITGK KAPGCVLSNP DQKGKMRRID CLRQADKVWR 130 140 150 160 170 180 LDLVMVILFK GIPLESTDGE RLVKAAQCGH PVLCVQPHHI GVAVKELDLY LAYFVRERDA 190 200 210 220 230 240 EQSGSPRTGM GSDQEDSKPI TLDTTDFQES FVTSGVFSVT ELIQVSRTPV VTGTGPNFSL 250 260 270 280 290 300 GELQGHLAYD LNPASTGLRR TLPSTSSSGS KRHKSGSMEE DVDTSPGGDY YTSPSSPTSS 310 320 330 340 350 360 SRNWTEDMEG GISSPVKKTE MDKSPFNSPS PQDSPRLSSF TQHHRPVIAV HSGIARSPHP 370 380 390 400 410 420 SSALHFPTTS ILPQTASTYF PHTAIRYPPH LNPQDPLKDL VSLACDPASQ QPGPLNGSGQ 430 440 450 460 470 480 LKMPSHCLSA QMLAPPPPGL PRLALPPATK PATTSEGGAT SPTSPSYSPP DTSPANRSFV 490 GLGPRDPAGI YQAQSWYLG « Hide
Isoform 2. Checksum: 99820E6484D655B3 Show »	FASTA	430	47,901
10 20 30 40 50 60 MDEFHPFIEA LLPHVRAFAY TWFNLQARKR KYFKKHEKRM SKDEERAVKD ELLGEKPEVK 70 80 90 100 110 120 QKWASRLLAK LRKDIRPECR EDFVLSITGK KAPGCVLSNP DQKGKMRRID CLRQADKVWR 130 140 150 160 170 180 LDLVMVILFK GIPLESTDGE RLVKAAQCGH PVLCVQPHHI GVAVKELDLY LAYFVRERDA 190 200 210 220 230 240 EQSGSPRTGM GSDQEDSKPI TLDTTDFQES FVTSGVFSVT ELIQVSRTPV VTGTGPNFSL 250 260 270 280 290 300 GELQGHLAYD LNPASTGLRR TLPSTSSSGS KRHKSGSMEE DVDTSPGGDY YTSPSSPTSS 310 320 330 340 350 360 SRNWTEDMEG GISSPVKKTE MDKSPFNSPS PQDSPRLSSF TQHHRPVIAV HSGIARSPHP 370 380 390 400 410 420 SSALHFPTTS ILPQTASTYF PHTAIRYPPH LNPQDPLKDL VSLACDPASQ QPGPPTLRPT 430 RPLQTVPLWD « Hide
Isoform 3. Checksum: EA00A93762A9FAC1 Show »	FASTA	406	45,413
10 20 30 40 50 60 MDEFHPFIEA LLPHVRAFAY TWFNLQARKR KYFKKHEKRM SKDEERAVKD ELLGEKPEVK 70 80 90 100 110 120 QKWASRLLAK LRKDIRPECR EDFVLSITGK KAPGCVLSNP DQKGKMRRID CLRQADKVWR 130 140 150 160 170 180 LDLVMVILFK GIPLESTDGE RLVKAAQCGH PVLCVQPHHI GVAVKELDLY LAYFVRERDT 190 200 210 220 230 240 TDFQESFVTS GVFSVTELIQ VSRTPVVTGT GPNFSLGELQ GHLAYDLNPA STGLRRTLPS 250 260 270 280 290 300 TSSSGSKRHK SGSMEEDVDT SPGGDYYTSP SSPTSSSRNW TEDMEGGISS PVKKTEMDKS 310 320 330 340 350 360 PFNSPSPQDS PRLSSFTQHH RPVIAVHSGI ARSPHPSSAL HFPTTSILPQ TASTYFPHTA 370 380 390 400 IRYPPHLNPQ DPLKDLVSLA CDPASQQPGP PTLRPTRPLQ TVPLWD « Hide
Isoform 5. Checksum: 239F7A15BBEA2539 Show »	FASTA	428	47,629
10 20 30 40 50 60 MYSSPLCLTQ DEFHPFIEAL LPHVRAFAYT WFNLQARKRK YFKKHEKRMS KDEERAVKDE 70 80 90 100 110 120 LLGEKPEVKQ KWASRLLAKL RKDIRPECRE DFVLSITGKK APGCVLSNPD QKGKMRRIDC 130 140 150 160 170 180 LRQADKVWRL DLVMVILFKG IPLESTDGER LVKAAQCGHP VLCVQPHHIG VAVKELDLYL 190 200 210 220 230 240 AYFVRERDAE QSGSPRTGMG SDQEDSKPIT LDTTDFQESF VTSGVFSVTE LIQVSRTPVV 250 260 270 280 290 300 TGTGPNFSLG ELQGHLAYDL NPASTGLRRT LPSTSSSGSK RHKSGSMEED VDTSPGGDYY 310 320 330 340 350 360 TSPSSPTSSS RNWTEDMEGG ISSPVKKTEM DKSPFNSPSP QDSPRLSSFT QHHRPVIAVH 370 380 390 400 410 420 SGIARSPHPS SALHFPTTSI LPQTASTYFP HTAIRYPPHL NPQDPLKDLV SLACDPASQQ PGPSWYLG « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A family of human CCAAT-box-binding proteins active in transcription and DNA replication: cloning and expression of multiple cDNAs." Santoro C., Mermod N., Andrews P.C., Tjian R. Nature 334:218-224(1988) [PubMed: 3398920] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), PARTIAL PROTEIN SEQUENCE.
[2]	"CTF5 -- a new transcriptional activator of the NFI/CTF family." Wenzelides S., Altmann S., Wendler W., Winnacker E.L. Nucleic Acids Res. 24:2416-2421(1996) [PubMed: 8710515] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Caudate nucleus and Uterus.
[4]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5). Tissue: Uterus.
[7]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-194; SER-333 AND SER-339, MASS SPECTROMETRY. Tissue: Epithelium.
[8]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-4; SER-194; SER-323; SER-333 AND SER-339, MASS SPECTROMETRY. Tissue: Epithelium.
[9]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-323 AND SER-339, MASS SPECTROMETRY.
[10]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-304; SER-305; SER-339 AND SER-343, MASS SPECTROMETRY.
[11]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-323, MASS SPECTROMETRY.
[12]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-294; SER-305; SER-323; SER-333 AND SER-339, MASS SPECTROMETRY.
[13]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed: 19369195] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-194, MASS SPECTROMETRY.
[14]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-333, MASS SPECTROMETRY. Tissue: T-cell.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	X12492 mRNA. Translation: CAA31012.1. X92857 mRNA. Translation: CAA63440.1. AK289885 mRNA. Translation: BAF82574.1. AK304816 mRNA. Translation: BAH14259.1. AC005514 Genomic DNA. Translation: AAC32593.1. AC005514 Genomic DNA. Translation: AAC32594.1. AC005551 Genomic DNA. Translation: AAC33190.1. AC005551 Genomic DNA. Translation: AAC33191.1. AC005551 Genomic DNA. Translation: AAC33192.1. AC005778 Genomic DNA. Translation: AAC62842.1. CH471139 Genomic DNA. Translation: EAW69325.1. CH471139 Genomic DNA. Translation: EAW69326.1. BC012120 mRNA. Translation: AAH12120.1.
IPI	IPI00029795. IPI00218037. IPI00218039. IPI00218041. IPI00941207.
PIR	B33416. S01038.
RefSeq	NP_005588.2. NP_995315.1.
UniGene	Hs.170131
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P08651. 5 interactions.
STRING	P08651.
PTM databases
PhosphoSite	P08651.
Proteomic databases
PRIDE	P08651.
Genome annotation databases
Ensembl	ENST00000269778; ENSP00000269778; ENSG00000141905; Homo sapiens. [Genome view]
GeneID	4782.
KEGG	hsa:4782.
UCSC	uc002lxo.1. human. uc002lxp.1. human.
Organism-specific databases
CTD	4782.
GeneCards	GC19P003310.
H-InvDB	HIX0014638.
HGNC	HGNC:7786. NFIC.
HPA	CAB004300. CAB004674.
MIM	600729. gene.
PharmGKB	PA31592.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15913.
HOVERGEN	P08651.
OMA	LKMPSHC.
OrthoDB	EOG9G4K8W.
PhylomeDB	P08651.
Enzyme and pathway databases
Pathway_Interaction_DB	hnf3apathway. FOXA1 transcription factor network.
Gene expression databases
ArrayExpress	P08651.
Bgee	P08651.
CleanEx	HS_NFIC.
Genevestigator	P08651.
GermOnline	ENSG00000141905. Homo sapiens.
Family and domain databases
InterPro	IPR000647. CTF/NFI. IPR020604. CTF/NFI_DNA-bd-dom. IPR019739. CTF/NFI_DNA-bd_CS. IPR019548. CTF/NFI_DNA-bd_N. IPR003619. MAD_homology1_Dwarfin-type. [Graphical view]
PANTHER	PTHR11492. CTF_NFI. 1 hit.
Pfam	PF00859. CTF_NFI. 1 hit. PF03165. MH1. 1 hit. PF10524. NfI_DNAbd_pre-N. 1 hit. [Graphical view]
SMART	SM00523. DWA. 1 hit. [Graphical view]
PROSITE	PS00349. CTF_NFI_1. 1 hit. PS51080. CTF_NFI_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	18440.
SOURCE	Search...

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Entry information

Entry name

NFIC_HUMAN

Accession

Primary (citable) accession number: P08651
Secondary accession number(s): A8K1H0

Q9UPK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	June 1, 2001
Last modified:	February 9, 2010
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Relevant documents

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents