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P08651 (NFIC_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 154. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear factor 1 C-type

Short name=NF1-C
Short name=Nuclear factor 1/C
Alternative name(s):
CCAAT-box-binding transcription factor
Short name=CTF
Nuclear factor I/C
Short name=NF-I/C
Short name=NFI-C
TGGCA-binding protein
Gene names
Name:NFIC
Synonyms:NFI
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length508 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Subunit structure

Binds DNA as a homodimer.

Subcellular location

Nucleus.

Sequence similarities

Belongs to the CTF/NF-I family.

Contains 1 CTF/NF-I DNA-binding domain.

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform 4 (identifier: P08651-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: P08651-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MYSSPLCLTQ → M
Isoform 2 (identifier: P08651-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MYSSPLCLTQ → M
     424-439: LNGSGQLKMPSHCLSA → PTLRPTRPLQTVPLWD
     440-508: Missing.
Isoform 3 (identifier: P08651-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MYSSPLCLTQ → M
     188-211: Missing.
     424-439: LNGSGQLKMPSHCLSA → PTLRPTRPLQTVPLWD
     440-508: Missing.
Isoform 5 (identifier: P08651-5)

The sequence of this isoform differs from the canonical sequence as follows:
     424-503: Missing.
Isoform 6 (identifier: P08651-6)

The sequence of this isoform differs from the canonical sequence as follows:
     424-508: LNGSGQLKMP...IYQAQSWYLG → PTLRPTRPLQTVPLWD
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 508508Nuclear factor 1 C-type
PRO_0000100199

Regions

DNA binding1 – 195195CTF/NF-I

Amino acid modifications

Modified residue11N-acetylmethionine Ref.12
Modified residue1941Phosphoserine Ref.12
Modified residue2941Phosphoserine Ref.10
Modified residue3231Phosphoserine Ref.10 Ref.12
Modified residue3331Phosphoserine Ref.7 Ref.10 Ref.11 Ref.12
Modified residue3371Phosphoserine Ref.12
Modified residue3391Phosphoserine Ref.10 Ref.12
Modified residue3431Phosphoserine Ref.8 Ref.12
Modified residue4871Phosphoserine Ref.12

Natural variations

Alternative sequence1 – 1010MYSSPLCLTQ → M in isoform 1, isoform 2 and isoform 3.
VSP_003552
Alternative sequence188 – 21124Missing in isoform 3.
VSP_003553
Alternative sequence424 – 50885LNGSG…SWYLG → PTLRPTRPLQTVPLWD in isoform 6.
VSP_047539
Alternative sequence424 – 50380Missing in isoform 5.
VSP_003554
Alternative sequence424 – 43916LNGSG…HCLSA → PTLRPTRPLQTVPLWD in isoform 2 and isoform 3.
VSP_003555
Alternative sequence440 – 50869Missing in isoform 2 and isoform 3.
VSP_003556
Natural variant4171A → S.
Corresponds to variant rs10412720 [ dbSNP | Ensembl ].
VAR_057656

Experimental info

Sequence conflict1301L → P in BAH12681. Ref.3
Sequence conflict2121D → V in CAA63440. Ref.2
Sequence conflict3291E → K in BAH12681. Ref.3
Sequence conflict3531H → Y in BAH12681. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 4 [UniParc].

Last modified June 1, 2001. Version 2.
Checksum: 3D0F97DA0F3AF992

FASTA50855,675
        10         20         30         40         50         60 
MYSSPLCLTQ DEFHPFIEAL LPHVRAFAYT WFNLQARKRK YFKKHEKRMS KDEERAVKDE 

        70         80         90        100        110        120 
LLGEKPEVKQ KWASRLLAKL RKDIRPECRE DFVLSITGKK APGCVLSNPD QKGKMRRIDC 

       130        140        150        160        170        180 
LRQADKVWRL DLVMVILFKG IPLESTDGER LVKAAQCGHP VLCVQPHHIG VAVKELDLYL 

       190        200        210        220        230        240 
AYFVRERDAE QSGSPRTGMG SDQEDSKPIT LDTTDFQESF VTSGVFSVTE LIQVSRTPVV 

       250        260        270        280        290        300 
TGTGPNFSLG ELQGHLAYDL NPASTGLRRT LPSTSSSGSK RHKSGSMEED VDTSPGGDYY 

       310        320        330        340        350        360 
TSPSSPTSSS RNWTEDMEGG ISSPVKKTEM DKSPFNSPSP QDSPRLSSFT QHHRPVIAVH 

       370        380        390        400        410        420 
SGIARSPHPS SALHFPTTSI LPQTASTYFP HTAIRYPPHL NPQDPLKDLV SLACDPASQQ 

       430        440        450        460        470        480 
PGPLNGSGQL KMPSHCLSAQ MLAPPPPGLP RLALPPATKP ATTSEGGATS PTSPSYSPPD 

       490        500 
TSPANRSFVG LGPRDPAGIY QAQSWYLG 

« Hide

Isoform 1 [UniParc].

Checksum: B6A59BD10B4B6D1B
Show »

FASTA49954,682
Isoform 2 [UniParc].

Checksum: 99820E6484D655B3
Show »

FASTA43047,901
Isoform 3 [UniParc].

Checksum: EA00A93762A9FAC1
Show »

FASTA40645,413
Isoform 5 [UniParc].

Checksum: 239F7A15BBEA2539
Show »

FASTA42847,629
Isoform 6 [UniParc].

Checksum: 5A9A6B88FAF84891
Show »

FASTA43948,894

References

« Hide 'large scale' references
[1]"A family of human CCAAT-box-binding proteins active in transcription and DNA replication: cloning and expression of multiple cDNAs."
Santoro C., Mermod N., Andrews P.C., Tjian R.
Nature 334:218-224(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), PARTIAL PROTEIN SEQUENCE.
[2]"CTF5 -- a new transcriptional activator of the NFI/CTF family."
Wenzelides S., Altmann S., Wendler W., Winnacker E.L.
Nucleic Acids Res. 24:2416-2421(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 6).
Tissue: Caudate nucleus, Heart and Uterus.
[4]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
Tissue: Uterus.
[7]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-333, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-343, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-294; SER-323; SER-333 AND SER-339, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-333, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[12]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-194; SER-323; SER-333; SER-337; SER-339; SER-343 AND SER-487, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X12492 mRNA. Translation: CAA31012.1.
X92857 mRNA. Translation: CAA63440.1.
AK289885 mRNA. Translation: BAF82574.1.
AK297867 mRNA. Translation: BAH12681.1.
AK304816 mRNA. Translation: BAH14259.1.
AC005514 Genomic DNA. Translation: AAC32593.1.
AC005514 Genomic DNA. Translation: AAC32594.1.
AC005551 Genomic DNA. Translation: AAC33190.1.
AC005551 Genomic DNA. Translation: AAC33191.1.
AC005551 Genomic DNA. Translation: AAC33192.1.
AC005778 Genomic DNA. Translation: AAC62842.1.
CH471139 Genomic DNA. Translation: EAW69325.1.
CH471139 Genomic DNA. Translation: EAW69326.1.
BC012120 mRNA. Translation: AAH12120.1.
CCDSCCDS12107.1. [P08651-5]
CCDS45914.1. [P08651-2]
CCDS58640.1. [P08651-3]
CCDS59330.1. [P08651-1]
CCDS59331.1. [P08651-6]
PIRB33416.
S01038.
RefSeqNP_001231931.1. NM_001245002.1. [P08651-1]
NP_001231933.1. NM_001245004.1. [P08651-6]
NP_001231934.1. NM_001245005.1. [P08651-3]
NP_005588.2. NM_005597.3. [P08651-5]
NP_995315.1. NM_205843.2. [P08651-2]
UniGeneHs.170131.

3D structure databases

ProteinModelPortalP08651.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110854. 15 interactions.
IntActP08651. 6 interactions.
MINTMINT-1439785.

PTM databases

PhosphoSiteP08651.

Polymorphism databases

DMDM14195672.

Proteomic databases

MaxQBP08651.
PaxDbP08651.
PRIDEP08651.

Protocols and materials databases

DNASU4782.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000341919; ENSP00000342194; ENSG00000141905. [P08651-5]
ENST00000346156; ENSP00000301935; ENSG00000141905. [P08651-4]
ENST00000395111; ENSP00000378543; ENSG00000141905. [P08651-3]
ENST00000443272; ENSP00000396843; ENSG00000141905. [P08651-1]
ENST00000586919; ENSP00000465177; ENSG00000141905. [P08651-4]
ENST00000589123; ENSP00000465655; ENSG00000141905. [P08651-2]
ENST00000590282; ENSP00000466647; ENSG00000141905. [P08651-6]
GeneID4782.
KEGGhsa:4782.
UCSCuc002lxo.3. human. [P08651-2]
uc002lxp.3. human. [P08651-5]
uc010xhh.2. human. [P08651-3]
uc010xhi.2. human. [P08651-1]

Organism-specific databases

CTD4782.
GeneCardsGC19P003310.
HGNCHGNC:7786. NFIC.
HPACAB004300.
CAB004674.
HPA052625.
MIM600729. gene.
neXtProtNX_P08651.
PharmGKBPA31592.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG252172.
HOVERGENHBG006561.
KOK09170.
OMAMEGGISP.
OrthoDBEOG7WDN2K.
PhylomeDBP08651.

Enzyme and pathway databases

SignaLinkP08651.

Gene expression databases

ArrayExpressP08651.
BgeeP08651.
CleanExHS_NFIC.
GenevestigatorP08651.

Family and domain databases

InterProIPR000647. CTF/NFI.
IPR020604. CTF/NFI_DNA-bd-dom.
IPR019739. CTF/NFI_DNA-bd_CS.
IPR019548. CTF/NFI_DNA-bd_N.
IPR003619. MAD_homology1_Dwarfin-type.
[Graphical view]
PANTHERPTHR11492. PTHR11492. 1 hit.
PfamPF00859. CTF_NFI. 1 hit.
PF03165. MH1. 1 hit.
PF10524. NfI_DNAbd_pre-N. 1 hit.
[Graphical view]
SMARTSM00523. DWA. 1 hit.
[Graphical view]
PROSITEPS00349. CTF_NFI_1. 1 hit.
PS51080. CTF_NFI_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNFIC. human.
GeneWikiNFIC_(gene).
GenomeRNAi4782.
NextBio18440.
PROP08651.
SOURCESearch...

Entry information

Entry nameNFIC_HUMAN
AccessionPrimary (citable) accession number: P08651
Secondary accession number(s): A8K1H0 expand/collapse secondary AC list , B7Z4U5, B7Z9C3, K7EMU1, P08652, Q14932, Q9UPJ3, Q9UPJ9, Q9UPK0, Q9UPK1
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 154 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM