P08650 (CO5_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Complement C5

Cleaved into the following chain:

1. C5a anaphylatoxin

Gene names

Name:

C5

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	77 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).
Subcellular location	Secreted.
Sequence similarities	Contains 1 anaphylatoxin-like domain.

Ontologies

Keywords
Biological process	Complement alternate pathway Complement pathway Immunity Inflammatory response Innate immunity
Cellular component	Secreted
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular calcium ion homeostasis Inferred from direct assay PubMed 10532591. Source: RGD chemotaxis Inferred from direct assay PubMed 10410979. Source: RGD complement activation Traceable author statement PubMed 11870622. Source: RGD complement activation, alternative pathway Inferred from electronic annotation. Source: UniProtKB-KW complement activation, classical pathway Inferred from electronic annotation. Source: UniProtKB-KW cytolysis Traceable author statement PubMed 11870622. Source: RGD glucose homeostasis Inferred from direct assay PubMed 10421654. Source: RGD in utero embryonic development Inferred from electronic annotation. Source: Compara induction of apoptosis Inferred from direct assay PubMed 11870622. Source: RGD leukocyte migration involved in inflammatory response Inferred from mutant phenotype PubMed 10843715. Source: RGD negative regulation of dopamine secretion Inferred from direct assay PubMed 18648551. Source: RGD negative regulation of macrophage chemotaxis Inferred from electronic annotation. Source: Compara negative regulation of norepinephrine secretion Inferred from direct assay PubMed 18648551. Source: RGD positive regulation of angiogenesis Inferred from electronic annotation. Source: Compara positive regulation of chemokine secretion Inferred from electronic annotation. Source: Compara positive regulation of chemotaxis Inferred from direct assay Ref.2PubMed 8755663. Source: RGD positive regulation vascular endothelial growth factor production Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular space Inferred from direct assay PubMed 9246574. Source: RGD membrane attack complex Traceable author statement PubMed 11870622. Source: RGD
Molecular_function	C5a anaphylatoxin chemotactic receptor binding Inferred from direct assay PubMed 10410979Ref.2. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 77	77	C5a anaphylatoxin		PRO_0000005996
Regions
	Domain	24 – 58	35	Anaphylatoxin-like
Amino acid modifications
	Disulfide bond	24 ↔ 50		By similarity
	Disulfide bond	25 ↔ 57		By similarity
	Disulfide bond	37 ↔ 58		By similarity
Experimental info
	Sequence conflict	55	1	N → K AA sequence Ref.1
	Sequence conflict	55	1	N → K AA sequence Ref.2
	Sequence conflict	61 – 63	3	ADK → DP AA sequence Ref.1
	Sequence conflict	63	1	K → H AA sequence Ref.2
	Sequence conflict	67 – 69	3	ESH → NES AA sequence Ref.2
	Sequence conflict	67 – 69	3	ESH → NQS AA sequence Ref.1
	Non-terminal residue	1	1
	Non-terminal residue	77	1

Sequences

	Sequence		Length	Mass (Da)
	P08650 [UniParc]. Last modified April 13, 2004. Version 2. Checksum: 14141F41CC38BD28	FASTA	77	8,981
	10 20 30 40 50 60 DLQLLHQKVE EQAAKYKHRV PKKCCYDGAR ENKYETCEQR VARVTIGPHC IRAFNECCTI 70 ADKIRKESHH KGMLLGR

References

[1]	"Characterization of rat C5a, a uniquely active spasmogen." Cui L.-X., Ferreri K., Hugli T.E. Complement 2:18-19(1985) Cited for: PROTEIN SEQUENCE.
[2]	"Primary structure and functional characterization of rat C5a: an anaphylatoxin with unusually high potency." Cui L.-X., Carney D.F., Hugli T.E. Protein Sci. 3:1169-1177(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, CHARACTERIZATION. Tissue: Serum.
[3]	"Nucleotide and corrected amino acid sequence of the functional recombinant rat anaphylatoxin C5a." Rothermel E., Rolf O., Goetze O., Zwirner J. Biochim. Biophys. Acta 1351:9-12(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Brown Norway, Lewis and Wistar. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X91892 mRNA. Translation: CAA62994.1.
IPI	IPI00764698.
PIR	A57689.
UniGene	Rn.21259.
3D structure databases
ProteinModelPortal	P08650.
SMR	P08650. Positions 4-65.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000060337.
Protein family/group databases
MEROPS	I39.952.
PTM databases
PhosphoSite	P08650.
Proteomic databases
PRIDE	P08650.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:2237. rat.
Organism-specific databases
RGD	2237. C5.
Phylogenomic databases
eggNOG	KOG1366.
InParanoid	P08650.
Gene expression databases
Genevestigator	P08650.
GermOnline	ENSRNOG00000018899. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.91.20. 1 hit.
InterPro	IPR000020. Anaphylatoxin/fibulin. IPR018081. Anaphylatoxin_. IPR001840. Anaphylatoxn. [Graphical view]
Pfam	PF01821. ANATO. 1 hit. [Graphical view]
PRINTS	PR00004. ANAPHYLATOXN.
SMART	SM00104. ANATO. 1 hit. [Graphical view]
SUPFAM	SSF47686. Anaphylatoxin. 1 hit.
PROSITE	PS00477. ALPHA_2_MACROGLOBULIN. Partial match. PS01177. ANAPHYLATOXIN_1. 1 hit. PS01178. ANAPHYLATOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CO5_RAT

Accession

Primary (citable) accession number: P08650
Secondary accession number(s): Q63078

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	April 13, 2004
Last modified:	April 3, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program