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Protein

Complement C5

Gene

C5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.By similarity

GO - Molecular functioni

  • C5a anaphylatoxin chemotactic receptor binding Source: RGD

GO - Biological processi

  • cellular calcium ion homeostasis Source: RGD
  • chemotaxis Source: RGD
  • complement activation, alternative pathway Source: UniProtKB-KW
  • complement activation, classical pathway Source: UniProtKB-KW
  • glucose homeostasis Source: RGD
  • inflammatory response Source: RGD
  • in utero embryonic development Source: Ensembl
  • leukocyte migration involved in inflammatory response Source: RGD
  • negative regulation of dopamine secretion Source: RGD
  • negative regulation of macrophage chemotaxis Source: Ensembl
  • negative regulation of norepinephrine secretion Source: RGD
  • positive regulation of angiogenesis Source: Ensembl
  • positive regulation of chemokine secretion Source: Ensembl
  • positive regulation of chemotaxis Source: RGD
  • positive regulation of vascular endothelial growth factor production Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Complement alternate pathway, Complement pathway, Immunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

ReactomeiREACT_295126. Activation of C3 and C5.
REACT_306851. Peptide ligand-binding receptors.
REACT_321635. Regulation of Complement cascade.
REACT_339234. G alpha (i) signalling events.
REACT_340240. Terminal pathway of complement.

Names & Taxonomyi

Protein namesi
Recommended name:
Complement C5
Cleaved into the following chain:
Gene namesi
Name:C5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2237. C5.

Subcellular locationi

GO - Cellular componenti

  • cell Source: GOC
  • extracellular exosome Source: Ensembl
  • extracellular space Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7777C5a anaphylatoxinPRO_0000005996Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi24 ↔ 50PROSITE-ProRule annotation
Disulfide bondi25 ↔ 57PROSITE-ProRule annotation
Disulfide bondi37 ↔ 58PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP08650.

PTM databases

PhosphoSiteiP08650.

Interactioni

Subunit structurei

The C5a anaphylatoxin interacts with C5AR1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025534.

Structurei

3D structure databases

ProteinModelPortaliP08650.
SMRiP08650. Positions 4-65.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 5835Anaphylatoxin-likePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni18 – 4730Involved in C5AR1 bindingBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 anaphylatoxin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1366.
InParanoidiP08650.

Family and domain databases

Gene3Di1.20.91.20. 1 hit.
InterProiIPR000020. Anaphylatoxin/fibulin.
IPR018081. Anaphylatoxin_comp_syst.
IPR001840. Anaphylatoxn_comp_syst_dom.
[Graphical view]
PfamiPF01821. ANATO. 1 hit.
[Graphical view]
PRINTSiPR00004. ANAPHYLATOXN.
SMARTiSM00104. ANATO. 1 hit.
[Graphical view]
SUPFAMiSSF47686. SSF47686. 1 hit.
PROSITEiPS01177. ANAPHYLATOXIN_1. 1 hit.
PS01178. ANAPHYLATOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08650-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
DLQLLHQKVE EQAAKYKHRV PKKCCYDGAR ENKYETCEQR VARVTIGPHC
60 70
IRAFNECCTI ADKIRKESHH KGMLLGR
Length:77
Mass (Da):8,981
Last modified:April 13, 2004 - v2
Checksum:i14141F41CC38BD28
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Sequence conflicti55 – 551N → K AA sequence (Ref. 1) Curated
Sequence conflicti55 – 551N → K AA sequence (PubMed:7987212).Curated
Sequence conflicti61 – 633ADK → DP AA sequence (Ref. 1) Curated
Sequence conflicti63 – 631K → H AA sequence (PubMed:7987212).Curated
Sequence conflicti67 – 693ESH → NES AA sequence (PubMed:7987212).Curated
Sequence conflicti67 – 693ESH → NQS AA sequence (Ref. 1) Curated
Non-terminal residuei77 – 771

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91892 mRNA. Translation: CAA62994.1.
PIRiA57689.
UniGeneiRn.21259.

Genome annotation databases

UCSCiRGD:2237. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91892 mRNA. Translation: CAA62994.1.
PIRiA57689.
UniGeneiRn.21259.

3D structure databases

ProteinModelPortaliP08650.
SMRiP08650. Positions 4-65.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025534.

PTM databases

PhosphoSiteiP08650.

Proteomic databases

PRIDEiP08650.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:2237. rat.

Organism-specific databases

RGDi2237. C5.

Phylogenomic databases

eggNOGiKOG1366.
InParanoidiP08650.

Enzyme and pathway databases

ReactomeiREACT_295126. Activation of C3 and C5.
REACT_306851. Peptide ligand-binding receptors.
REACT_321635. Regulation of Complement cascade.
REACT_339234. G alpha (i) signalling events.
REACT_340240. Terminal pathway of complement.

Family and domain databases

Gene3Di1.20.91.20. 1 hit.
InterProiIPR000020. Anaphylatoxin/fibulin.
IPR018081. Anaphylatoxin_comp_syst.
IPR001840. Anaphylatoxn_comp_syst_dom.
[Graphical view]
PfamiPF01821. ANATO. 1 hit.
[Graphical view]
PRINTSiPR00004. ANAPHYLATOXN.
SMARTiSM00104. ANATO. 1 hit.
[Graphical view]
SUPFAMiSSF47686. SSF47686. 1 hit.
PROSITEiPS01177. ANAPHYLATOXIN_1. 1 hit.
PS01178. ANAPHYLATOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of rat C5a, a uniquely active spasmogen."
    Cui L.-X., Ferreri K., Hugli T.E.
    Complement 2:18-19(1985)
    Cited for: PROTEIN SEQUENCE.
  2. "Primary structure and functional characterization of rat C5a: an anaphylatoxin with unusually high potency."
    Cui L.-X., Carney D.F., Hugli T.E.
    Protein Sci. 3:1169-1177(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, CHARACTERIZATION.
    Tissue: Serum.
  3. "Nucleotide and corrected amino acid sequence of the functional recombinant rat anaphylatoxin C5a."
    Rothermel E., Rolf O., Goetze O., Zwirner J.
    Biochim. Biophys. Acta 1351:9-12(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Brown Norway, Lewis and Wistar.
    Tissue: Liver.

Entry informationi

Entry nameiCO5_RAT
AccessioniPrimary (citable) accession number: P08650
Secondary accession number(s): Q63078
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: April 13, 2004
Last modified: June 24, 2015
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.