Integrin alpha-5 precursor - Homo sapiens (Human)

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Reviewed, UniProtKB/Swiss-Prot P08648 (ITA5_HUMAN)

Last modified December 15, 2009. Version 116. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Integrin alpha-5
Alternative name(s):
    Fibronectin receptor subunit alpha
    Integrin alpha-F
    VLA-5
    CD49 antigen-like family member E
    CD_antigen=CD49e
Cleaved into the following 2 chains:
    1- Recommended name:
            Integrin alpha-5 heavy chain
    2- Recommended name:
            Integrin alpha-5 light chain

Gene names

Name:	ITGA5
Synonyms:	FNRA

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	1049 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Subunit structure	Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5 and NISCH. Interacts with RAB21 and COMP. Interacts with HIV-1 Tat. Ref.7 Ref.8 Ref.10 Ref.11
Subcellular location	Membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats.

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Ontologies

Keywords
Biological process	Cell adhesion Host-virus interaction
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Repeat Signal Transmembrane
Ligand	Calcium
Molecular function	Integrin Receptor
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	integrin-mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW wound healing, spreading of epidermal cells Inferred from expression pattern. Source: UniProtKB
Cellular component	integrin complex Traceable author statement. Source: ProtInc ruffle Traceable author statement. Source: HGNC
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 41

Ref.6

Chain

42 – 1049

1008

Integrin alpha-5

PRO_0000016249

Chain

42 – 894

853

Integrin alpha-5 heavy chain

PRO_0000016250

Chain

895 – 1049

155

Integrin alpha-5 light chain

PRO_0000016251

Regions

Topological domain

42 – 995

954

Extracellular Potential

Transmembrane

996 – 1021

Potential

Topological domain

1022 – 1049

Cytoplasmic Potential

Repeat

57 – 119

FG-GAP 1

Repeat

131 – 198

FG-GAP 2

Repeat

199 – 256

FG-GAP 3

Repeat

269 – 322

FG-GAP 4

Repeat

323 – 389

FG-GAP 5

Repeat

390 – 449

FG-GAP 6

Repeat

453 – 505

FG-GAP 7

Calcium binding

334 – 342

Potential

Calcium binding

401 – 409

Potential

Calcium binding

465 – 473

Potential

Region

1021 – 1028

Interaction with HPS5

Motif

1024 – 1028

GFFKR motif

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

182

N-linked (GlcNAc...) Potential

Glycosylation

297

N-linked (GlcNAc...) Ref.13 Ref.14

Glycosylation

307

N-linked (GlcNAc...) Ref.13 Ref.14

Glycosylation

316

N-linked (GlcNAc...) Potential

Glycosylation

524

N-linked (GlcNAc...) Potential

Glycosylation

530

N-linked (GlcNAc...) Potential

Glycosylation

593

N-linked (GlcNAc...) Potential

Glycosylation

609

N-linked (GlcNAc...) Potential

Glycosylation

675

N-linked (GlcNAc...) Ref.13

Glycosylation

682

N-linked (GlcNAc...) Ref.14

Glycosylation

712

N-linked (GlcNAc...) Potential

Glycosylation

724

N-linked (GlcNAc...) Potential

Glycosylation

771

N-linked (GlcNAc...) Ref.14

Glycosylation

773

N-linked (GlcNAc...) Ref.13 Ref.14

Glycosylation

774

N-linked (GlcNAc...) Ref.14

Glycosylation

868

N-linked (GlcNAc...) Potential

Disulfide bond

Disulfide bond

Disulfide bond

Disulfide bond

By similarity

Disulfide bond

528 ↔ 584

By similarity

Disulfide bond

Disulfide bond

Disulfide bond

Interchain (between heavy and light chains) Ref.9

Disulfide bond

911 ↔ 916

Ref.9

Natural variations

Natural variant

585

R → I: dbSNP rs12318746.

VAR_049631

Experimental info

Sequence conflict

L → V Ref.1

Sequence conflict

L → V Ref.3

Sequence conflict

L → V Ref.1

Sequence conflict

L → V Ref.3

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Sequences

Sequence

Length

Mass (Da)

Tools

P08648-1 [UniParc].

Last modified October 10, 2002. Version 2.
Checksum: 6B4D558D4F739CBA
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FASTA

1,049

114,536

        10         20         30         40         50         60 
MGSRTPESPL HAVQLRWGPR RRPPLLPLLL LLLPPPPRVG GFNLDAEAPA VLSGPPGSFF 

        70         80         90        100        110        120 
GFSVEFYRPG TDGVSVLVGA PKANTSQPGV LQGGAVYLCP WGASPTQCTP IEFDSKGSRL 

       130        140        150        160        170        180 
LESSLSSSEG EEPVEYKSLQ WFGATVRAHG SSILACAPLY SWRTEKEPLS DPVGTCYLST 

       190        200        210        220        230        240 
DNFTRILEYA PCRSDFSWAA GQGYCQGGFS AEFTKTGRVV LGGPGSYFWQ GQILSATQEQ 

       250        260        270        280        290        300 
IAESYYPEYL INLVQGQLQT RQASSIYDDS YLGYSVAVGE FSGDDTEDFV AGVPKGNLTY 

       310        320        330        340        350        360 
GYVTILNGSD IRSLYNFSGE QMASYFGYAV AATDVNGDGL DDLLVGAPLL MDRTPDGRPQ 

       370        380        390        400        410        420 
EVGRVYVYLQ HPAGIEPTPT LTLTGHDEFG RFGSSLTPLG DLDQDGYNDV AIGAPFGGET 

       430        440        450        460        470        480 
QQGVVFVFPG GPGGLGSKPS QVLQPLWAAS HTPDFFGSAL RGGRDLDGNG YPDLIVGSFG 

       490        500        510        520        530        540 
VDKAVVYRGR PIVSASASLT IFPAMFNPEE RSCSLEGNPV ACINLSFCLN ASGKHVADSI 

       550        560        570        580        590        600 
GFTVELQLDW QKQKGGVRRA LFLASRQATL TQTLLIQNGA REDCREMKIY LRNESEFRDK 

       610        620        630        640        650        660 
LSPIHIALNF SLDPQAPVDS HGLRPALHYQ SKSRIEDKAQ ILLDCGEDNI CVPDLQLEVF 

       670        680        690        700        710        720 
GEQNHVYLGD KNALNLTFHA QNVGEGGAYE AELRVTAPPE AEYSGLVRHP GNFSSLSCDY 

       730        740        750        760        770        780 
FAVNQSRLLV CDLGNPMKAG ASLWGGLRFT VPHLRDTKKT IQFDFQILSK NLNNSQSDVV 

       790        800        810        820        830        840 
SFRLSVEAQA QVTLNGVSKP EAVLFPVSDW HPRDQPQKEE DLGPAVHHVY ELINQGPSSI 

       850        860        870        880        890        900 
SQGVLELSCP QALEGQQLLY VTRVTGLNCT TNHPINPKGL ELDPEGSLHH QQKREAPSRS 

       910        920        930        940        950        960 
SASSGPQILK CPEAECFRLR CELGPLHQQE SQSLQLHFRV WAKTFLQREH QPFSLQCEAV 

       970        980        990       1000       1010       1020 
YKALKMPYRI LPRQLPQKER QVATAVQWTK AEGSYGVPLW IIILAILFGL LLLGLLIYIL 

      1030       1040 
YKLGFFKRSL PYGTAMEKAQ LKPPATSDA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Amino acid sequence of the human fibronectin receptor." Argraves W.S., Suzuki S., Arai H., Thompson K., Pierschbacher M.D., Ruoslahti E. J. Cell Biol. 105:1183-1190(1987) [PubMed: 2958481] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[3]	"The alpha 5 beta 1 fibronectin receptor. Characterization of the alpha 5 gene promoter." Birkenmeier T.M., McQuillan J.J., Boedeker E.D., Argraves W.S., Ruoslahti E., Dean D.C. J. Biol. Chem. 266:20544-20549(1991) [PubMed: 1834647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-73.
[4]	"Comparison of cDNA-derived protein sequences of the human fibronectin and vitronectin receptor alpha-subunits and platelet glycoprotein IIb." Fitzgerald L.A., Poncz M., Steiner B., Rall S.C., Bennett J.S., Phillips D.R. Biochemistry 26:8158-8165(1987) [PubMed: 2450560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-1049.
[5]	"cDNA sequences from the alpha subunit of the fibronectin receptor predict a transmembrane domain and a short cytoplasmic peptide." Argraves W.S., Pytela R., Suzuki S., Millan J.L., Pierschbacher M.D., Ruoslahti E. J. Biol. Chem. 261:12922-12924(1986) [PubMed: 2944883] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 821-1049.
[6]	"The very late antigen family of heterodimers is part of a superfamily of molecules involved in adhesion and embryogenesis." Takada Y., Strominger J.L., Hemler M.E. Proc. Natl. Acad. Sci. U.S.A. 84:3239-3243(1987) [PubMed: 3033641] [Abstract] Cited for: PROTEIN SEQUENCE OF 42-55.
[7]	"The Tat protein of human immunodeficiency virus type-1 promotes vascular cell growth and locomotion by engaging the alpha5beta1 and alphavbeta3 integrins and by mobilizing sequestered basic fibroblast growth factor." Barillari G., Sgadari C., Fiorelli V., Samaniego F., Colombini S., Manzari V., Modesti A., Nair B.C., Cafaro A., Stuerzl M., Ensoli B. Blood 94:663-672(1999) [PubMed: 10397733] [Abstract] Cited for: INTERACTION WITH HIV-1 TAT.
[8]	"Insulin receptor substrate 4 associates with the protein IRAS." Sano H., Liu S.C.H., Lane W.S., Piletz J.E., Lienhard G.E. J. Biol. Chem. 277:19439-19447(2002) [PubMed: 11912194] [Abstract] Cited for: INTERACTION WITH NISCH.
[9]	"Mass spectrometric based mapping of the disulfide bonding patterns of integrin alpha chains." Krokhin O.V., Cheng K., Sousa S.L., Ens W., Standing K.G., Wilkins J.A. Biochemistry 42:12950-12959(2003) [PubMed: 14596610] [Abstract] Cited for: DISULFIDE BONDS.
[10]	"Cartilage oligomeric matrix protein/thrombospondin 5 supports chondrocyte attachment through interaction with integrins." Chen F.-H., Thomas A.O., Hecht J.T., Goldring M.B., Lawler J. J. Biol. Chem. 280:32655-32661(2005) [PubMed: 16051604] [Abstract] Cited for: INTERACTION WITH COMP.
[11]	"Small GTPase Rab21 regulates cell adhesion and controls endosomal traffic of beta1-integrins." Pellinen T., Arjonen A., Vuoriluoto K., Kallio K., Fransen J.A.M., Ivaska J. J. Cell Biol. 173:767-780(2006) [PubMed: 16754960] [Abstract] Cited for: INTERACTION WITH RAB21.
[12]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[13]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-297; ASN-307; ASN-675 AND ASN-773, MASS SPECTROMETRY. Tissue: Liver.
[14]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed: 19349973] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-297; ASN-307; ASN-682; ASN-771; ASN-773 AND ASN-774, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	X06256 mRNA. Translation: CAA29601.1. BC008786 mRNA. Translation: AAH08786.1. M13918 mRNA. Translation: AAA52467.1. Sequence problems.
IPI	IPI00306604.
PIR	A27079.
RefSeq	NP_002196.2.
UniGene	Hs.505654
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P08648.
PTM databases
GlycoSuiteDB	P08648.
PhosphoSite	P08648.
Proteomic databases
PeptideAtlas	P08648.
PRIDE	P08648.
Genome annotation databases
Ensembl	ENST00000293379; ENSP00000293379; ENSG00000161638; Homo sapiens. [Genome view]
GeneID	3678.
KEGG	hsa:3678.
UCSC	uc001sga.1. human.
Organism-specific databases
CTD	3678.
GeneCards	GC12M053075.
H-InvDB	HIX0010697.
HGNC	HGNC:6141. ITGA5.
HPA	CAB009008. HPA002642.
MIM	135620. gene.
PharmGKB	PA29941.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HBG402992.
HOVERGEN	P08648.
InParanoid	P08648.
OMA	MKIYLRN.
OrthoDB	EOG9CVJT3.
Enzyme and pathway databases
Pathway_Interaction_DB	angiopoietinreceptor_pathway. Angiopoietin receptor Tie2-mediated signaling. syndecan_2_pathway. Syndecan-2-mediated signaling events. syndecan_4_pathway. Syndecan-4-mediated signaling events. lymphangiogenesis_pathway. VEGFR3 signaling in lymphatic endothelium.
Reactome	REACT_13552. Integrin cell surface interactions. REACT_604. Hemostasis. REACT_6900. Signaling in Immune system.
Gene expression databases
ArrayExpress	P08648.
Bgee	P08648.
CleanEx	HS_ITGA5.
Genevestigator	P08648.
GermOnline	ENSG00000161638. Homo sapiens.
Family and domain databases
InterPro	IPR013517. FG-GAP. IPR013519. Int_alpha_beta-p. IPR000413. Integrin_alpha. IPR013649. Integrin_alpha-2. IPR013513. Integrin_alpha_C. IPR018184. Integrin_alpha_C_CS. [Graphical view]
Pfam	PF01839. FG-GAP. 3 hits. PF00357. Integrin_alpha. 1 hit. PF08441. Integrin_alpha2. 1 hit. [Graphical view]
PRINTS	PR01185. INTEGRINA.
SMART	SM00191. Int_alpha. 5 hits. [Graphical view]
PROSITE	PS00242. INTEGRIN_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	14395.
SOURCE	Search...

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Entry information

Entry name

ITA5_HUMAN

Accession

Primary (citable) accession number: P08648
Secondary accession number(s): Q96HA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	October 10, 2002
Last modified:	December 15, 2009
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents