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Protein

Ras-like protein 1

Gene

ras1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Participates in the process of sexual differentiation and the determination of cell shape. Essential for mating and for recognition of the mating pheromone, but not for vegetative growth. Does not regulate the intracellular cAMP level. Regulates two downstream pathways, namely the byr2/byr1/spk1 mitogen-activated protein kinase cascade and the cdc42 small G protein pathway. The former is relevant to mating and sporulation, whereas the latter is relevant to mating, cell growth and cell morphology.

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 22GTPBy similarity8
Nucleotide bindingi62 – 66GTPBy similarity5
Nucleotide bindingi121 – 124GTPBy similarity4

GO - Molecular functioni

  • CTP binding Source: PomBase
  • GDP binding Source: PomBase
  • GTPase activity Source: PomBase
  • GTP binding Source: PomBase
  • UTP binding Source: PomBase

GO - Biological processi

  • Cdc42 protein signal transduction Source: PomBase
  • conjugation with cellular fusion Source: PomBase
  • induction of conjugation with cellular fusion Source: PomBase
  • MAPK cascade involved in ascospore formation Source: PomBase
  • MAPK cascade involved in conjugation with cellular fusion Source: PomBase
  • meiotic cell cycle Source: PomBase
  • mitotic cell cycle G1 arrest in response to pheromone Source: PomBase
  • pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: PomBase
  • positive regulation of conjugation with cellular fusion Source: PomBase
  • positive regulation of protein localization to nucleus Source: PomBase
  • protein localization to plasma membrane Source: PomBase
  • regulation of establishment or maintenance of cell polarity regulating cell shape Source: PomBase
  • signal transduction involved in conjugation with cellular fusion Source: PomBase

Keywordsi

Biological processCell shape
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-like protein 1
Gene namesi
Name:ras1
ORF Names:SPAC17H9.09c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC17H9.09c.
PomBaseiSPAC17H9.09c. ras1.

Subcellular locationi

GO - Cellular componenti

  • barrier septum Source: PomBase
  • Cdc42 GTPase complex Source: PomBase
  • cell division site Source: PomBase
  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • intracellular Source: PomBase
  • nucleus Source: PomBase
  • plasma membrane Source: PomBase

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000826781 – 216Ras-like protein 1Add BLAST216
PropeptideiPRO_0000281333217 – 219Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei216Cysteine methyl esterBy similarity1
Lipidationi216S-farnesyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiP08647.
PRIDEiP08647.

PTM databases

SwissPalmiP08647.

Interactioni

Subunit structurei

Scd1, scd2, cdc42, and ras1, in its GTP-bound state, act cooperatively to form a protein complex.

Protein-protein interaction databases

BioGridi278753. 32 interactors.
DIPiDIP-61182N.
MINTiMINT-4687066.
STRINGi4896.SPAC17H9.09c.1.

Structurei

3D structure databases

ProteinModelPortaliP08647.
SMRiP08647.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi37 – 45Effector region9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

HOGENOMiHOG000233973.
InParanoidiP08647.
KOiK07827.
OMAiYAQTYKL.
OrthoDBiEOG092C4VY0.
PhylomeDBiP08647.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiView protein in Pfam
PF00071. Ras. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51421. RAS. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08647-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSTYLREYK LVVVGDGGVG KSALTIQLIQ SHFVDEYDPT IEDSYRKKCE
60 70 80 90 100
IDGEGALLDV LDTAGQEEYS AMREQYMRTG EGFLLVYNIT SRSSFDEIST
110 120 130 140 150
FYQQILRVKD KDTFPVVLVA NKCDLEAERV VSRAEGEQLA KSMHCLYVET
160 170 180 190 200
SAKLRLNVEE AFYSLVRTIR RYNKSEEKGF QNKQAVQTAQ VPASTAKRAS
210
AVNNSKTEDE VSTKCCVIC
Length:219
Mass (Da):24,734
Last modified:July 15, 1998 - v2
Checksum:i109A0C468663F535
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6L → M in CAA27399 (PubMed:3084798).Curated1
Sequence conflicti57L → V in CAA26191 (PubMed:4006903).Curated1
Sequence conflicti60V → L in CAA26191 (PubMed:4006903).Curated1
Sequence conflicti81E → Q in CAA26191 (PubMed:4006903).Curated1
Sequence conflicti134 – 136AEG → RER in CAA26191 (PubMed:4006903).Curated3
Sequence conflicti181Q → H in CAA26191 (PubMed:4006903).Curated1
Sequence conflicti188T → I in CAA26191 (PubMed:4006903).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02331 Genomic DNA. Translation: CAA26191.1.
X03771 Genomic DNA. Translation: CAA27399.1.
CU329670 Genomic DNA. Translation: CAB11218.1.
PIRiA22715. TVBYSR.
T37875. TVBYPR.
T45545.
RefSeqiNP_593579.1. NM_001019011.2.

Genome annotation databases

EnsemblFungiiSPAC17H9.09c.1; SPAC17H9.09c.1:pep; SPAC17H9.09c.
GeneIDi2542285.
KEGGispo:SPAC17H9.09c.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRAS_SCHPO
AccessioniPrimary (citable) accession number: P08647
Secondary accession number(s): O13806
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: July 15, 1998
Last modified: July 5, 2017
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families