Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ras-like protein 1

Gene

Ras85D

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (By similarity). Plays a role in eye development by regulating cell growth, survival of postmitotic ommatidial cells and differentiation of photoreceptor cells (PubMed:11290305). During larval development, mediates Ptth/tor signaling leading to the production of ecdysone, a hormone required for the initiation of metamorphosis (PubMed:19965758).By similarity2 Publications

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17GTPBy similarity8
Nucleotide bindingi57 – 61GTPBy similarity5
Nucleotide bindingi116 – 119GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: FlyBase
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • anterior/posterior axis specification, embryo Source: FlyBase
  • border follicle cell migration Source: FlyBase
  • cell fate determination Source: FlyBase
  • cell fate specification Source: FlyBase
  • cell proliferation Source: FlyBase
  • cellular response to starvation Source: FlyBase
  • chorion-containing eggshell pattern formation Source: FlyBase
  • compound eye development Source: FlyBase
  • defense response to virus Source: FlyBase
  • determination of adult lifespan Source: FlyBase
  • dorsal closure Source: UniProtKB
  • dorsal closure, spreading of leading edge cells Source: UniProtKB
  • establishment or maintenance of apical/basal cell polarity Source: FlyBase
  • eye-antennal disc morphogenesis Source: FlyBase
  • G1/S transition of mitotic cell cycle Source: FlyBase
  • gastrulation Source: FlyBase
  • heart development Source: FlyBase
  • hemocyte migration Source: FlyBase
  • imaginal disc-derived wing morphogenesis Source: FlyBase
  • imaginal disc-derived wing vein morphogenesis Source: FlyBase
  • instar larval development Source: FlyBase
  • intestinal stem cell homeostasis Source: FlyBase
  • Malpighian tubule development Source: FlyBase
  • MAPK cascade Source: UniProtKB
  • metamorphosis Source: FlyBase
  • negative regulation of apoptotic process Source: FlyBase
  • negative regulation of apoptotic signaling pathway Source: FlyBase
  • negative regulation of autophagy Source: FlyBase
  • negative regulation of compound eye retinal cell programmed cell death Source: FlyBase
  • negative regulation of gene expression Source: UniProtKB
  • negative regulation of macroautophagy Source: FlyBase
  • oocyte axis specification Source: FlyBase
  • ovarian follicle cell development Source: FlyBase
  • peripheral nervous system development Source: FlyBase
  • photoreceptor cell development Source: UniProtKB
  • photoreceptor cell morphogenesis Source: FlyBase
  • positive regulation of cell growth Source: FlyBase
  • positive regulation of cell proliferation Source: FlyBase
  • positive regulation of cell size Source: FlyBase
  • positive regulation of hemocyte proliferation Source: FlyBase
  • positive regulation of MAPK cascade Source: FlyBase
  • positive regulation of photoreceptor cell differentiation Source: FlyBase
  • primary branching, open tracheal system Source: FlyBase
  • protein import into nucleus Source: FlyBase
  • Ras protein signal transduction Source: FlyBase
  • regulation of apoptotic process Source: FlyBase
  • regulation of cell size Source: FlyBase
  • regulation of growth Source: FlyBase
  • regulation of hemocyte differentiation Source: FlyBase
  • regulation of multicellular organism growth Source: FlyBase
  • sevenless signaling pathway Source: FlyBase
  • stem cell differentiation Source: FlyBase
  • stem cell fate commitment Source: FlyBase
  • stem cell proliferation Source: FlyBase
  • TORC1 signaling Source: FlyBase
  • torso signaling pathway Source: FlyBase
  • trachea development Source: FlyBase
  • tracheal outgrowth, open tracheal system Source: FlyBase
  • wing disc morphogenesis Source: FlyBase

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-1433557 Signaling by SCF-KIT
R-DME-171007 p38MAPK events
R-DME-179812 GRB2 events in EGFR signaling
R-DME-180336 SHC1 events in EGFR signaling
R-DME-1963640 GRB2 events in ERBB2 signaling
R-DME-210993 Tie2 Signaling
R-DME-2179392 EGFR Transactivation by Gastrin
R-DME-375165 NCAM signaling for neurite out-growth
R-DME-3928662 EPHB-mediated forward signaling
R-DME-432553 Phosphorylation of PER and TIM
R-DME-442982 Ras activation upon Ca2+ influx through NMDA receptor
R-DME-5218921 VEGFR2 mediated cell proliferation
R-DME-5621575 CD209 (DC-SIGN) signaling
R-DME-5654693 FRS-mediated FGFR1 signaling
R-DME-5654699 SHC-mediated cascade:FGFR2
R-DME-5654700 FRS-mediated FGFR2 signaling
R-DME-5654704 SHC-mediated cascade:FGFR3
R-DME-5654706 FRS-mediated FGFR3 signaling
R-DME-5654712 FRS-mediated FGFR4 signaling
R-DME-5658442 Regulation of RAS by GAPs
R-DME-5673000 RAF activation
R-DME-5673001 RAF/MAP kinase cascade
R-DME-5674135 MAP2K and MAPK activation
R-DME-5675221 Negative regulation of MAPK pathway
R-DME-6798695 Neutrophil degranulation
R-DME-8849471 PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases
SignaLinkiP08646

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-like protein 1
Short name:
Dras1
Alternative name(s):
Dmras85D
Gene namesi
Name:Ras85D
Synonyms:Ras1
ORF Names:CG9375
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0003205 Ras85D

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown in the prothoracic gland (PG) delays the onset of pupariation by prolonging the L3 larval stage.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi12G → V: Rough eyes characterized by the presence of additional R7 photoreceptor cells. Normal survival of postmitotic ommatidial cells but differentiation into photoreceptor cells is limited to R8 cells. Less severe Rough eye phenotype; when associated with E-38. Normal eyes; when associated with C-40. 1 Publication1
Mutagenesisi38D → E: Mild rough eye phenotype; when associated with V-12. 1 Publication1
Mutagenesisi40Y → C: Impaired cell growth, survival of postmitotic ommatidial cells and differentiation into R8 photoreceptors. Normal eye morphology; when associated with V-12. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000826651 – 186Ras-like protein 1Add BLAST186
PropeptideiPRO_0000281313187 – 189Removed in mature formCurated3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei186Cysteine methyl esterCurated1
Lipidationi186S-geranylgeranyl cysteine1 Publication1

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

PaxDbiP08646
PRIDEiP08646

Expressioni

Tissue specificityi

Expressed in the posterior termini of the embryo, restricted mainly to the embryonic central nervous system, and in the eye imaginal disk.1 Publication

Gene expression databases

BgeeiFBgn0003205
GenevisibleiP08646 DM

Interactioni

Protein-protein interaction databases

BioGridi66301, 232 interactors
DIPiDIP-23541N
STRINGi7227.FBpp0081600

Structurei

3D structure databases

ProteinModelPortaliP08646
SMRiP08646
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 40Effector region9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00860000133672
InParanoidiP08646
KOiK07827
OMAiQSWAVNM
OrthoDBiEOG091G0UAU
PhylomeDBiP08646

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR020849 Small_GTPase_Ras-type
PANTHERiPTHR24070 PTHR24070, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51421 RAS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08646-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET
60 70 80 90 100
CLLDILDTAG QEEYSAMRDQ YMRTGEGFLL VFAVNSAKSF EDIGTYREQI
110 120 130 140 150
KRVKDAEEVP MVLVGNKCDL ASWNVNNEQA REVAKQYGIP YIETSAKTRM
160 170 180
GVDDAFYTLV REIRKDKDNK GRRGRKMNKP NRRFKCKML
Length:189
Mass (Da):21,594
Last modified:July 1, 1989 - v2
Checksum:i14236DCD65EACCD2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11A → P in AAA28846 (PubMed:6430564).Curated1
Sequence conflicti44 – 45VV → RF in AAA28846 (PubMed:6430564).Curated2
Sequence conflicti84V → I in AAA28846 (PubMed:6430564).Curated1
Sequence conflicti102R → H in AAA28846 (PubMed:6430564).Curated1
Sequence conflicti114V → A in AAA28846 (PubMed:6430564).Curated1
Sequence conflicti182R → C in AAA28846 (PubMed:6430564).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16429, M16123, M16428 Genomic DNA Translation: AAA28847.1
K01960 mRNA Translation: AAA28846.1
AF186648 Genomic DNA Translation: AAF15514.1
AE014297 Genomic DNA Translation: AAF54388.1
AY089541 mRNA Translation: AAL90279.1
AY094888 mRNA Translation: AAM11241.1
X73219 Genomic DNA Translation: CAA51689.1
PIRiA29048 TVFF85
S35097
RefSeqiNP_476699.1, NM_057351.5
UniGeneiDm.4812

Genome annotation databases

EnsemblMetazoaiFBtr0082122; FBpp0081600; FBgn0003205
GeneIDi41140
KEGGidme:Dmel_CG9375

Similar proteinsi

Entry informationi

Entry nameiRAS1_DROME
AccessioniPrimary (citable) accession number: P08646
Secondary accession number(s): Q9V448
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: July 1, 1989
Last modified: May 23, 2018
This is version 187 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health