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Protein

Ras-like protein 3

Gene

R

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays a role in photoreceptor cell determination.1 Publication

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17GTPBy similarity8
Nucleotide bindingi57 – 61GTPBy similarity5
Nucleotide bindingi116 – 119GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: FlyBase
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • adherens junction assembly Source: FlyBase
  • cell adhesion Source: FlyBase
  • dorsal closure Source: FlyBase
  • establishment of ommatidial planar polarity Source: FlyBase
  • germ-line stem cell population maintenance Source: FlyBase
  • hemocyte migration Source: FlyBase
  • ovarian follicle cell stalk formation Source: FlyBase
  • positive regulation of cell-cell adhesion Source: FlyBase
  • Rap protein signal transduction Source: FlyBase
  • regulation of cell shape Source: FlyBase
  • regulation of embryonic cell shape Source: FlyBase
  • substrate-dependent cell migration, cell extension Source: FlyBase
  • visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-170984. ARMS-mediated activation.
R-DME-354192. Integrin alphaIIb beta3 signaling.
R-DME-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-DME-372708. p130Cas linkage to MAPK signaling for integrins.
R-DME-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-DME-392517. Rap1 signalling.
R-DME-6798695. Neutrophil degranulation.
R-DME-8875555. MET activates RAP1 and RAC1.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-like protein 3
Alternative name(s):
Protein roughened
Gene namesi
Name:R
Synonyms:RAP, Rap1, ras3, Ras62B
ORF Names:CG1956
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0004636. R.

Subcellular locationi

GO - Cellular componenti

  • cell leading edge Source: FlyBase
  • lipid particle Source: FlyBase
  • plasma membrane Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Flies exhibit disruption of the early stages of photoreceptor cell determination, adult eyes lack the R7 cell.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000301911 – 181Ras-like protein 3Add BLAST181
PropeptideiPRO_0000030192182 – 184Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei181Cysteine methyl esterBy similarity1
Lipidationi181S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

PaxDbiP08645.
PRIDEiP08645.

Expressioni

Gene expression databases

BgeeiFBgn0004636.
ExpressionAtlasiP08645. baseline.
GenevisibleiP08645. DM.

Interactioni

Protein-protein interaction databases

BioGridi63773. 34 interactors.
IntActiP08645. 1 interactor.
STRINGi7227.FBpp0072746.

Structurei

3D structure databases

ProteinModelPortaliP08645.
SMRiP08645.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 40Effector region9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133678.
InParanoidiP08645.
KOiK04353.
OMAiRDSTHPR.
OrthoDBiEOG091G0OOG.
PhylomeDBiP08645.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08645-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREYKIVVLG SGGVGKSALT VQFVQCIFVE KYDPTIEDSY RKQVEVDGQQ
60 70 80 90 100
CMLEILDTAG TEQFTAMRDL YMKNGQGFVL VYSITAQSTF NDLQDLREQI
110 120 130 140 150
LRVKDTDDVP MVLVGNKCDL EEERVVGKEL GKNLATQFNC AFMETSAKAK
160 170 180
VNVNDIFYDL VRQINKKSPE KKQKKPKKSL CVLL
Length:184
Mass (Da):20,863
Last modified:August 1, 1992 - v2
Checksum:i9A55889B61FEDE13
GO

Sequence cautioni

The sequence CAA26131 differs from that shown. Reason: Frameshift at positions 76, 96, 160 and 180.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45 – 49EVDGQ → KVNER in CAA26131 (PubMed:3926484).Curated5
Sequence conflicti56 – 57LD → VN in CAA26131 (PubMed:3926484).Curated2
Sequence conflicti69D → N in CAA26131 (PubMed:3926484).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti157F → L in roughened mutants. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02200 Genomic DNA. Translation: CAA26131.1. Frameshift.
M80535 Genomic DNA. Translation: AAA28845.1.
AF186654 Genomic DNA. Translation: AAF15520.1.
AE014296 Genomic DNA. Translation: AAF47583.1.
AY121688 mRNA. Translation: AAM52015.1.
DQ138746 Genomic DNA. Translation: ABA86352.1.
PIRiA41217.
S07187. TVFFR3.
RefSeqiNP_001189023.1. NM_001202094.2.
NP_001261295.1. NM_001274366.1.
NP_476857.1. NM_057509.5.
UniGeneiDm.822.

Genome annotation databases

EnsemblMetazoaiFBtr0072867; FBpp0072746; FBgn0004636.
FBtr0303154; FBpp0292253; FBgn0004636.
FBtr0332671; FBpp0304917; FBgn0004636.
GeneIDi38244.
KEGGidme:Dmel_CG1956.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02200 Genomic DNA. Translation: CAA26131.1. Frameshift.
M80535 Genomic DNA. Translation: AAA28845.1.
AF186654 Genomic DNA. Translation: AAF15520.1.
AE014296 Genomic DNA. Translation: AAF47583.1.
AY121688 mRNA. Translation: AAM52015.1.
DQ138746 Genomic DNA. Translation: ABA86352.1.
PIRiA41217.
S07187. TVFFR3.
RefSeqiNP_001189023.1. NM_001202094.2.
NP_001261295.1. NM_001274366.1.
NP_476857.1. NM_057509.5.
UniGeneiDm.822.

3D structure databases

ProteinModelPortaliP08645.
SMRiP08645.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi63773. 34 interactors.
IntActiP08645. 1 interactor.
STRINGi7227.FBpp0072746.

Proteomic databases

PaxDbiP08645.
PRIDEiP08645.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0072867; FBpp0072746; FBgn0004636.
FBtr0303154; FBpp0292253; FBgn0004636.
FBtr0332671; FBpp0304917; FBgn0004636.
GeneIDi38244.
KEGGidme:Dmel_CG1956.

Organism-specific databases

CTDi38244.
FlyBaseiFBgn0004636. R.

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133678.
InParanoidiP08645.
KOiK04353.
OMAiRDSTHPR.
OrthoDBiEOG091G0OOG.
PhylomeDBiP08645.

Enzyme and pathway databases

ReactomeiR-DME-170984. ARMS-mediated activation.
R-DME-354192. Integrin alphaIIb beta3 signaling.
R-DME-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-DME-372708. p130Cas linkage to MAPK signaling for integrins.
R-DME-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-DME-392517. Rap1 signalling.
R-DME-6798695. Neutrophil degranulation.
R-DME-8875555. MET activates RAP1 and RAC1.

Miscellaneous databases

ChiTaRSiR. fly.
GenomeRNAii38244.
PROiP08645.

Gene expression databases

BgeeiFBgn0004636.
ExpressionAtlasiP08645. baseline.
GenevisibleiP08645. DM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAS3_DROME
AccessioniPrimary (citable) accession number: P08645
Secondary accession number(s): Q2XYB6, Q540V3, Q9V3N0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 1, 1992
Last modified: November 30, 2016
This is version 177 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.