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Protein

GTPase HRas

Gene

HRAS

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17GTP8
Nucleotide bindingi57 – 61GTP5
Nucleotide bindingi116 – 119GTP4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-GGA-1169092. Activation of RAS in B cells.
R-GGA-1250347. SHC1 events in ERBB4 signaling.
R-GGA-1433557. Signaling by SCF-KIT.
R-GGA-171007. p38MAPK events.
R-GGA-179812. GRB2 events in EGFR signaling.
R-GGA-180336. SHC1 events in EGFR signaling.
R-GGA-186763. Downstream signal transduction.
R-GGA-1963640. GRB2 events in ERBB2 signaling.
R-GGA-210993. Tie2 Signaling.
R-GGA-2179392. EGFR Transactivation by Gastrin.
R-GGA-2424491. DAP12 signaling.
R-GGA-2871796. FCERI mediated MAPK activation.
R-GGA-375165. NCAM signaling for neurite out-growth.
R-GGA-3928662. EPHB-mediated forward signaling.
R-GGA-442982. Ras activation uopn Ca2+ infux through NMDA receptor.
R-GGA-5218921. VEGFR2 mediated cell proliferation.
R-GGA-5621575. CD209 (DC-SIGN) signaling.
R-GGA-5654688. SHC-mediated cascade:FGFR1.
R-GGA-5654693. FRS-mediated FGFR1 signaling.
R-GGA-5654699. SHC-mediated cascade:FGFR2.
R-GGA-5654700. FRS-mediated FGFR2 signaling.
R-GGA-5654704. SHC-mediated cascade:FGFR3.
R-GGA-5654706. FRS-mediated FGFR3 signaling.
R-GGA-5654712. FRS-mediated FGFR4 signaling.
R-GGA-5654719. SHC-mediated cascade:FGFR4.
R-GGA-5658442. Regulation of RAS by GAPs.
R-GGA-5673000. RAF activation.
R-GGA-5673001. RAF/MAP kinase cascade.
R-GGA-5674135. MAP2K and MAPK activation.
R-GGA-5675221. Negative regulation of MAPK pathway.
R-GGA-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.
R-GGA-8851805. MET activates RAS signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase HRas
Alternative name(s):
H-Ras-1
Transforming protein p21
c-H-ras
p21ras
Cleaved into the following chain:
Gene namesi
Name:HRAS
Synonyms:HRAS1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi164R → A: Loss of GTP-binding activity. 1 Publication1

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004343071 – 186GTPase HRasAdd BLAST186
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00000430022 – 186GTPase HRas, N-terminally processedAdd BLAST185
PropeptideiPRO_0000043003187 – 189Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylthreonine; in GTPase HRas, N-terminally processedBy similarity1
Modified residuei118S-nitrosocysteineBy similarity1
Lipidationi181S-palmitoyl cysteineBy similarity1
Lipidationi184S-palmitoyl cysteineBy similarity1
Modified residuei186Cysteine methyl esterBy similarity1
Lipidationi186S-farnesyl cysteineBy similarity1

Post-translational modificationi

Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.By similarity

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Palmitate, Prenylation, S-nitrosylation

Proteomic databases

PaxDbiP08642.
PRIDEiP08642.

Expressioni

Gene expression databases

BgeeiENSGALG00000006885.
ExpressionAtlasiP08642. differential.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000038222.

Structurei

3D structure databases

ProteinModelPortaliP08642.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni166 – 185Hypervariable regionAdd BLAST20

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 40Effector region9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133672.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiP08642.
KOiK02833.
OMAiDCMNCKC.
OrthoDBiEOG091G0UAU.
PhylomeDBiP08642.
TreeFamiTF312796.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08642-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET
60 70 80 90 100
CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHQYREQI
110 120 130 140 150
KRVKDSDDVP MVLVGNKCDL PARTVETRQA QDLARSYGIP YIETSAKTRQ
160 170 180
GVEDAFYTLV REIRQHKLRK LNPPDESGPG CMNCKCVIS
Length:189
Mass (Da):21,365
Last modified:August 13, 1987 - v1
Checksum:i0E7BA6C929482387
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03578 mRNA. Translation: CAA27258.1.
PIRiA24617. TVCHRS.
RefSeqiNP_990623.1. NM_205292.1.
UniGeneiGga.3849.

Genome annotation databases

EnsembliENSGALT00000061350; ENSGALP00000051306; ENSGALG00000029260.
ENSGALT00000079859; ENSGALP00000056118; ENSGALG00000034526.
GeneIDi396229.
KEGGigga:396229.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03578 mRNA. Translation: CAA27258.1.
PIRiA24617. TVCHRS.
RefSeqiNP_990623.1. NM_205292.1.
UniGeneiGga.3849.

3D structure databases

ProteinModelPortaliP08642.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000038222.

Proteomic databases

PaxDbiP08642.
PRIDEiP08642.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000061350; ENSGALP00000051306; ENSGALG00000029260.
ENSGALT00000079859; ENSGALP00000056118; ENSGALG00000034526.
GeneIDi396229.
KEGGigga:396229.

Organism-specific databases

CTDi3265.

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133672.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiP08642.
KOiK02833.
OMAiDCMNCKC.
OrthoDBiEOG091G0UAU.
PhylomeDBiP08642.
TreeFamiTF312796.

Enzyme and pathway databases

ReactomeiR-GGA-1169092. Activation of RAS in B cells.
R-GGA-1250347. SHC1 events in ERBB4 signaling.
R-GGA-1433557. Signaling by SCF-KIT.
R-GGA-171007. p38MAPK events.
R-GGA-179812. GRB2 events in EGFR signaling.
R-GGA-180336. SHC1 events in EGFR signaling.
R-GGA-186763. Downstream signal transduction.
R-GGA-1963640. GRB2 events in ERBB2 signaling.
R-GGA-210993. Tie2 Signaling.
R-GGA-2179392. EGFR Transactivation by Gastrin.
R-GGA-2424491. DAP12 signaling.
R-GGA-2871796. FCERI mediated MAPK activation.
R-GGA-375165. NCAM signaling for neurite out-growth.
R-GGA-3928662. EPHB-mediated forward signaling.
R-GGA-442982. Ras activation uopn Ca2+ infux through NMDA receptor.
R-GGA-5218921. VEGFR2 mediated cell proliferation.
R-GGA-5621575. CD209 (DC-SIGN) signaling.
R-GGA-5654688. SHC-mediated cascade:FGFR1.
R-GGA-5654693. FRS-mediated FGFR1 signaling.
R-GGA-5654699. SHC-mediated cascade:FGFR2.
R-GGA-5654700. FRS-mediated FGFR2 signaling.
R-GGA-5654704. SHC-mediated cascade:FGFR3.
R-GGA-5654706. FRS-mediated FGFR3 signaling.
R-GGA-5654712. FRS-mediated FGFR4 signaling.
R-GGA-5654719. SHC-mediated cascade:FGFR4.
R-GGA-5658442. Regulation of RAS by GAPs.
R-GGA-5673000. RAF activation.
R-GGA-5673001. RAF/MAP kinase cascade.
R-GGA-5674135. MAP2K and MAPK activation.
R-GGA-5675221. Negative regulation of MAPK pathway.
R-GGA-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.
R-GGA-8851805. MET activates RAS signaling.

Miscellaneous databases

PROiP08642.

Gene expression databases

BgeeiENSGALG00000006885.
ExpressionAtlasiP08642. differential.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRASH_CHICK
AccessioniPrimary (citable) accession number: P08642
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: November 30, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.