Flocculation protein FLO11 precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P08640 (MUC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 107. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Flocculation protein FLO11
Short name=Flocculin-11

Alternative name(s):
Mucin-like protein 1

Gene names

Name:	MUC1
Synonyms:	FLO11, MAL5, S1, S2
Ordered Locus Names:	YIR019C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca²⁺-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells. Activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose. Also involved in cell-substrate adhesion, haploid invasive growth, diploid pseudohyphae formation and biofilm (flor) development. Ref.5 Ref.6 Ref.8 Ref.9 Ref.11 Ref.12
Subcellular location	Secreted › cell wall Probable. Membrane; Lipid-anchor › GPI-anchor Potential Ref.5 Ref.7 Ref.8.
Domain	The number of the intragenic tandem repeats varies between different S.cerevisiae strains. There is a linear correlation between protein size and the extend of adhesion: the more repeats, the stronger the adhesion properties and the greater the fraction of flocculating cells By similarity.
Post-translational modification	Extensively O-mannosylated. The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer By similarity.
Sequence similarities	Belongs to the MUC1 family.

Ontologies

Keywords
Cellular component	Cell wall Membrane Secreted
Domain	Repeat Signal
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	biofilm formation Inferred from mutant phenotype PubMed 11157168 PubMed 19160455. Source: SGD cell-cell adhesion Inferred from mutant phenotype PubMed 21875945. Source: SGD flocculation Inferred from mutant phenotype PubMed 19160455. Source: SGD invasive growth in response to glucose limitation Inferred from mutant phenotype PubMed 12150916 PubMed 19087208 Ref.6. Source: SGD pseudohyphal growth Inferred from mutant phenotype Ref.6. Source: SGD
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell cellular bud neck Inferred from direct assay PubMed 19799621. Source: SGD extracellular region Inferred from direct assay PubMed 20619652. Source: SGD plasma membrane Inferred from direct assay Ref.8. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

Potential

Chain

22 – 1346

1325

Flocculation protein FLO11

PRO_0000019586

Propeptide

1347 – 1367

Removed in mature form Potential

PRO_0000019587

Regions

Repeat

210 – 219

1-1

Repeat

220 – 229

1-2

Repeat

230 – 239

1-3

Repeat

240 – 249

1-4

Repeat

262 – 274

2-1

Repeat

275 – 287

2-2

Repeat

313 – 327

3-1

Repeat

328 – 342

3-2

Repeat

343 – 354

4-1

Repeat

355 – 369

3-3

Repeat

370 – 381

4-2

Repeat

382 – 393

4-3

Repeat

394 – 408

3-4

Repeat

409 – 420

4-4

Repeat

421 – 432

4-5

Repeat

433 – 444

4-6

Repeat

445 – 456

4-7

Repeat

457 – 471

3-5

Repeat

472 – 483

4-8

Repeat

484 – 498

3-6

Repeat

499 – 510

4-9

Repeat

511 – 525

3-7

Repeat

526 – 540

3-8

Repeat

541 – 552

4-10

Repeat

568 – 579

4-11

Repeat

580 – 594

3-9

Repeat

595 – 609

3-10

Repeat

610 – 625

3-11

Repeat

625 – 636

4-12

Repeat

637 – 651

3-12

Repeat

652 – 666

3-13

Repeat

667 – 681

3-14

Repeat

682 – 693

4-13

Repeat

694 – 705

4-14

Repeat

706 – 720

3-15

Repeat

721 – 735

3-16

Repeat

736 – 750

3-17

Repeat

751 – 762

4-15

Repeat

763 – 777

3-18

Repeat

778 – 792

3-19

Repeat

808 – 822

3-21

Repeat

838 – 852

3-20

Repeat

865 – 879

3-22

Repeat

937 – 968

5-1

Repeat

981 – 1012

5-2

Repeat

1088 – 1119

5-3

Region

210 – 249

4 X 10 AA repeats, Ser/Thr-rich

Region

262 – 287

2 X 13 AA repeats, Thr-rich

Region

313 – 852

540

22 X 15 AA approximate repeats, Ser-rich

Region

343 – 762

420

15 X 12 AA repeats, Ser/Thr-rich

Region

937 – 1119

183

3 X 32 AA tandem repeats, Thr-rich

Amino acid modifications

Lipidation

1346

GPI-anchor amidated glycine Potential

Glycosylation

817

N-linked (GlcNAc...) Potential

Glycosylation

874

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P08640 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 91C00E2DBD61AA9D
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FASTA

1,367

136,111

        10         20         30         40         50         60 
MQRPFLLAYL VLSLLFNSAL GFPTALVPRG SSEGTSCNSI VNGCPNLDFN WHMDQQNIMQ 

        70         80         90        100        110        120 
YTLDVTSVSW VQDNTYQITI HVKGKENIDL KYLWSLKIIG VTGPKGTVQL YGYNENTYLI 

       130        140        150        160        170        180 
DNPTDFTATF EVYATQDVNS CQVWMPNFQI QFEYLQGSAA QYASSWQWGT TSFDLSTGCN 

       190        200        210        220        230        240 
NYDNQGHSQT DFPGFYWNID CDNNCGGTKS STTTSSTSES STTTSSTSES STTTSSTSES 

       250        260        270        280        290        300 
STTTSSTSES STSSSTTAPA TPTTTSCTKE KPTPPTTTSC TKEKPTPPHH DTTPCTKKKT 

       310        320        330        340        350        360 
TTSKTCTKKT TTPVPTPSSS TTESSSAPVP TPSSSTTESS SAPVTSSTTE SSSAPVPTPS 

       370        380        390        400        410        420 
SSTTESSSAP VTSSTTESSS APVTSSTTES SSAPVPTPSS STTESSSAPV TSSTTESSSA 

       430        440        450        460        470        480 
PVTSSTTESS SAPVTSSTTE SSSAPVTSST TESSSAPVPT PSSSTTESSS APVTSSTTES 

       490        500        510        520        530        540 
SSAPVPTPSS STTESSSAPV TSSTTESSSA PVPTPSSSTT ESSSAPAPTP SSSTTESSSA 

       550        560        570        580        590        600 
PVTSSTTESS SAPVPTPSSS TTESSSTPVT SSTTESSSAP VPTPSSSTTE SSSAPVPTPS 

       610        620        630        640        650        660 
SSTTESSSAP APTPSSSTTE SSSAPVTSST TESSSAPVPT PSSSTTESSS APVPTPSSST 

       670        680        690        700        710        720 
TESSSAPVPT PSSSTTESSS APVTSSTTES SSAPVTSSTT ESSSAPVPTP SSSTTESSSA 

       730        740        750        760        770        780 
PVPTPSSSTT ESSSAPVPTP SSSTTESSSA PVTSSTTESS SAPVPTPSSS TTESSSAPVP 

       790        800        810        820        830        840 
TPSSSTTESS SAPVPTPSSS TTESSVAPVP TPSSSSNITS SAPSSTPFSS STESSSVPVP 

       850        860        870        880        890        900 
TPSSSTTESS SAPVSSSTTE SSVAPVPTPS SSSNITSSAP SSIPFSSTTE SFSTGTTVTP 

       910        920        930        940        950        960 
SSSKYPGSQT ETSVSSTTET TIVPTKTTTS VTTPSTTTIT TTVCSTGTNS AGETTSGCSP 

       970        980        990       1000       1010       1020 
KTVTTTVPTT TTTSVTTSST TTITTTVCST GTNSAGETTS GCSPKTITTT VPCSTSPSET 

      1030       1040       1050       1060       1070       1080 
ASESTTTSPT TPVTTVVSTT VVTTEYSTST KPGGEITTTF VTKNIPTTYL TTIAPTPSVT 

      1090       1100       1110       1120       1130       1140 
TVTNFTPTTI TTTVCSTGTN SAGETTSGCS PKTVTTTVPC STGTGEYTTE ATTLVTTAVT 

      1150       1160       1170       1180       1190       1200 
TTVVTTESST GTNSAGKTTT GYTTKSVPTT YVTTLAPSAP VTPATNAVPT TITTTECSAA 

      1210       1220       1230       1240       1250       1260 
TNAAGETTSV CSAKTIVSSA SAGENTAPSA TTPVTTAIPT TVITTESSVG TNSAGETTTG 

      1270       1280       1290       1300       1310       1320 
YTTKSIPTTY ITTLIPGSNG AKNYETVATA TNPISIKTTS QLATTASASS VAPVVTSPSL 

      1330       1340       1350       1360 
TGPLQSASGS AVATYSVPSI SSTYQGAANI KVLGNFMWLL LALPVVF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Gene fusion is a possible mechanism underlying the evolution of STA1." Yamashita I., Nakamura M., Fukui S. J. Bacteriol. 169:2142-2149(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-242 AND 762-1331.
[4]	"Similar short elements in the 5' regions of the STA2 and SGA genes from Saccharomyces cerevisiae." Pardo J.M., Ianez E., Zalacain M., Claros M.G., Jimenez A. FEBS Lett. 239:179-184(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31. Strain: SPX101-1C.
[5]	"FLO11, a yeast gene related to the STA genes, encodes a novel cell surface flocculin." Lo W.S., Dranginis A.M. J. Bacteriol. 178:7144-7151(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Muc1, a mucin-like protein that is regulated by Mss10, is critical for pseudohyphal differentiation in yeast." Lambrechts M.G., Bauer F.F., Marmur J., Pretorius I.S. Proc. Natl. Acad. Sci. U.S.A. 93:8419-8424(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Amino acid residues in the omega-minus region participate in cellular localization of yeast glycosylphosphatidylinositol-attached proteins." Hamada K., Terashima H., Arisawa M., Yabuki N., Kitada K. J. Bacteriol. 181:3886-3889(1999) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[8]	"A Saccharomyces gene family involved in invasive growth, cell-cell adhesion, and mating." Guo B., Styles C.A., Feng Q., Fink G.R. Proc. Natl. Acad. Sci. U.S.A. 97:12158-12163(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[9]	"Characteristics of Flo11-dependent flocculation in Saccharomyces cerevisiae." Bayly J.C., Douglas L.M., Pretorius I.S., Bauer F.F., Dranginis A.M. FEMS Yeast Res. 5:1151-1156(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[10]	"Intragenic tandem repeats generate functional variability." Verstrepen K.J., Jansen A., Lewitter F., Fink G.R. Nat. Genet. 37:986-990(2005) [PubMed] [Europe PMC] [Abstract] Cited for: REPEATS.
[11]	"Expression and characterization of the flocculin Flo11/Muc1, a Saccharomyces cerevisiae mannoprotein with homotypic properties of adhesion." Douglas L.M., Li L., Yang Y., Dranginis A.M. Eukaryot. Cell 6:2214-2221(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, GLYCOSYLATION.
[12]	"Differential Flo8p-dependent regulation of FLO1 and FLO11 for cell-cell and cell-substrate adherence of S.cerevisiae S288c." Fichtner L., Schulze F., Braus G.H. Mol. Microbiol. 66:1276-1289(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: Sigma 1278B.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z38061 Genomic DNA. Translation: CAA86176.1. M16164 Genomic DNA. Translation: AAA35014.1. M16165 Genomic DNA. Translation: AAA35015.1. X13857 Genomic DNA. Translation: CAA32069.1. BK006942 Genomic DNA. Translation: DAA08566.1.
PIR	S48478.
RefSeq	NP_012284.3. NM_001179541.3.
3D structure databases
ProteinModelPortal	P08640.
ModBase	Search...
Protein-protein interaction databases
IntAct	P08640. 2 interactions.
MINT	MINT-2782681.
Proteomic databases
PeptideAtlas	P08640.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIR019C; YIR019C; YIR019C.
GeneID	854836.
KEGG	sce:YIR019C. sce:YIR026C.
Organism-specific databases
CYGD	YIR019c.
SGD	S000001458. MUC1.
Phylogenomic databases
eggNOG	NOG12793.
GeneTree	ENSGT00700000104174.
KO	K01178. K14819.
OMA	ADQFTYV.
OrthoDB	EOG4BS0WG.
Gene expression databases
Genevestigator	P08640.
GermOnline	YIR019C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018789. Uncharacterised_Flo11-rel_N. [Graphical view]
Pfam	PF10182. Flo11. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977713.

Entry information

Entry name

MUC1_YEAST

Accession

Primary (citable) accession number: P08640
Secondary accession number(s): D6VVV0, P08068

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents