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P08640

- MUC1_YEAST

UniProt

P08640 - MUC1_YEAST

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Protein
Flocculation protein FLO11
Gene
MUC1, FLO11, MAL5, S1, S2, YIR019C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca2+-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells. Activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose. Also involved in cell-substrate adhesion, haploid invasive growth, diploid pseudohyphae formation and biofilm (flor) development.6 Publications

GO - Biological processi

  1. biofilm formation Source: SGD
  2. filamentous growth Source: SGD
  3. flocculation Source: SGD
  4. invasive growth in response to glucose limitation Source: SGD
  5. pseudohyphal growth Source: SGD
  6. single organismal cell-cell adhesion Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31439-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flocculation protein FLO11
Short name:
Flocculin-11
Alternative name(s):
Mucin-like protein 1
Gene namesi
Name:MUC1
Synonyms:FLO11, MAL5, S1, S2
Ordered Locus Names:YIR019C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

CYGDiYIR019c.
SGDiS000001458. MUC1.

Subcellular locationi

Secretedcell wall Inferred. Membrane; Lipid-anchorGPI-anchor Reviewed prediction 3 Publications

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. cell wall Source: UniProtKB-SubCell
  3. cellular bud neck Source: SGD
  4. extracellular region Source: SGD
  5. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed prediction
Add
BLAST
Chaini22 – 13461325Flocculation protein FLO11
PRO_0000019586Add
BLAST
Propeptidei1347 – 136721Removed in mature form Reviewed prediction
PRO_0000019587Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi817 – 8171N-linked (GlcNAc...) Reviewed prediction
Glycosylationi874 – 8741N-linked (GlcNAc...) Reviewed prediction
Lipidationi1346 – 13461GPI-anchor amidated glycine Reviewed prediction

Post-translational modificationi

Extensively O-mannosylated.
The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer By similarity.

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PeptideAtlasiP08640.

Expressioni

Gene expression databases

GenevestigatoriP08640.

Interactioni

Protein-protein interaction databases

BioGridi35011. 78 interactions.
IntActiP08640. 2 interactions.
MINTiMINT-2782681.

Structurei

3D structure databases

ProteinModelPortaliP08640.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati210 – 219101-1
Repeati220 – 229101-2
Repeati230 – 239101-3
Repeati240 – 249101-4
Repeati262 – 274132-1
Add
BLAST
Repeati275 – 287132-2
Add
BLAST
Repeati313 – 327153-1
Add
BLAST
Repeati328 – 342153-2
Add
BLAST
Repeati343 – 354124-1
Add
BLAST
Repeati355 – 369153-3
Add
BLAST
Repeati370 – 381124-2
Add
BLAST
Repeati382 – 393124-3
Add
BLAST
Repeati394 – 408153-4
Add
BLAST
Repeati409 – 420124-4
Add
BLAST
Repeati421 – 432124-5
Add
BLAST
Repeati433 – 444124-6
Add
BLAST
Repeati445 – 456124-7
Add
BLAST
Repeati457 – 471153-5
Add
BLAST
Repeati472 – 483124-8
Add
BLAST
Repeati484 – 498153-6
Add
BLAST
Repeati499 – 510124-9
Add
BLAST
Repeati511 – 525153-7
Add
BLAST
Repeati526 – 540153-8
Add
BLAST
Repeati541 – 552124-10
Add
BLAST
Repeati568 – 579124-11
Add
BLAST
Repeati580 – 594153-9
Add
BLAST
Repeati595 – 609153-10
Add
BLAST
Repeati610 – 625163-11
Add
BLAST
Repeati625 – 636124-12
Add
BLAST
Repeati637 – 651153-12
Add
BLAST
Repeati652 – 666153-13
Add
BLAST
Repeati667 – 681153-14
Add
BLAST
Repeati682 – 693124-13
Add
BLAST
Repeati694 – 705124-14
Add
BLAST
Repeati706 – 720153-15
Add
BLAST
Repeati721 – 735153-16
Add
BLAST
Repeati736 – 750153-17
Add
BLAST
Repeati751 – 762124-15
Add
BLAST
Repeati763 – 777153-18
Add
BLAST
Repeati778 – 792153-19
Add
BLAST
Repeati808 – 822153-21
Add
BLAST
Repeati838 – 852153-20
Add
BLAST
Repeati865 – 879153-22
Add
BLAST
Repeati937 – 968325-1
Add
BLAST
Repeati981 – 1012325-2
Add
BLAST
Repeati1088 – 1119325-3
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni210 – 249404 X 10 AA repeats, Ser/Thr-rich
Add
BLAST
Regioni262 – 287262 X 13 AA repeats, Thr-rich
Add
BLAST
Regioni313 – 85254022 X 15 AA approximate repeats, Ser-rich
Add
BLAST
Regioni343 – 76242015 X 12 AA repeats, Ser/Thr-rich
Add
BLAST
Regioni937 – 11191833 X 32 AA tandem repeats, Thr-rich
Add
BLAST

Domaini

The number of the intragenic tandem repeats varies between different S.cerevisiae strains. There is a linear correlation between protein size and the extend of adhesion: the more repeats, the stronger the adhesion properties and the greater the fraction of flocculating cells By similarity.

Sequence similaritiesi

Belongs to the MUC1 family.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00730000112629.
KOiK01178.
OMAiADQFTYV.
OrthoDBiEOG7S7SS8.

Family and domain databases

InterProiIPR018789. Uncharacterised_Flo11-rel_N.
[Graphical view]
PfamiPF10182. Flo11. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08640-1 [UniParc]FASTAAdd to Basket

« Hide

MQRPFLLAYL VLSLLFNSAL GFPTALVPRG SSEGTSCNSI VNGCPNLDFN     50
WHMDQQNIMQ YTLDVTSVSW VQDNTYQITI HVKGKENIDL KYLWSLKIIG 100
VTGPKGTVQL YGYNENTYLI DNPTDFTATF EVYATQDVNS CQVWMPNFQI 150
QFEYLQGSAA QYASSWQWGT TSFDLSTGCN NYDNQGHSQT DFPGFYWNID 200
CDNNCGGTKS STTTSSTSES STTTSSTSES STTTSSTSES STTTSSTSES 250
STSSSTTAPA TPTTTSCTKE KPTPPTTTSC TKEKPTPPHH DTTPCTKKKT 300
TTSKTCTKKT TTPVPTPSSS TTESSSAPVP TPSSSTTESS SAPVTSSTTE 350
SSSAPVPTPS SSTTESSSAP VTSSTTESSS APVTSSTTES SSAPVPTPSS 400
STTESSSAPV TSSTTESSSA PVTSSTTESS SAPVTSSTTE SSSAPVTSST 450
TESSSAPVPT PSSSTTESSS APVTSSTTES SSAPVPTPSS STTESSSAPV 500
TSSTTESSSA PVPTPSSSTT ESSSAPAPTP SSSTTESSSA PVTSSTTESS 550
SAPVPTPSSS TTESSSTPVT SSTTESSSAP VPTPSSSTTE SSSAPVPTPS 600
SSTTESSSAP APTPSSSTTE SSSAPVTSST TESSSAPVPT PSSSTTESSS 650
APVPTPSSST TESSSAPVPT PSSSTTESSS APVTSSTTES SSAPVTSSTT 700
ESSSAPVPTP SSSTTESSSA PVPTPSSSTT ESSSAPVPTP SSSTTESSSA 750
PVTSSTTESS SAPVPTPSSS TTESSSAPVP TPSSSTTESS SAPVPTPSSS 800
TTESSVAPVP TPSSSSNITS SAPSSTPFSS STESSSVPVP TPSSSTTESS 850
SAPVSSSTTE SSVAPVPTPS SSSNITSSAP SSIPFSSTTE SFSTGTTVTP 900
SSSKYPGSQT ETSVSSTTET TIVPTKTTTS VTTPSTTTIT TTVCSTGTNS 950
AGETTSGCSP KTVTTTVPTT TTTSVTTSST TTITTTVCST GTNSAGETTS 1000
GCSPKTITTT VPCSTSPSET ASESTTTSPT TPVTTVVSTT VVTTEYSTST 1050
KPGGEITTTF VTKNIPTTYL TTIAPTPSVT TVTNFTPTTI TTTVCSTGTN 1100
SAGETTSGCS PKTVTTTVPC STGTGEYTTE ATTLVTTAVT TTVVTTESST 1150
GTNSAGKTTT GYTTKSVPTT YVTTLAPSAP VTPATNAVPT TITTTECSAA 1200
TNAAGETTSV CSAKTIVSSA SAGENTAPSA TTPVTTAIPT TVITTESSVG 1250
TNSAGETTTG YTTKSIPTTY ITTLIPGSNG AKNYETVATA TNPISIKTTS 1300
QLATTASASS VAPVVTSPSL TGPLQSASGS AVATYSVPSI SSTYQGAANI 1350
KVLGNFMWLL LALPVVF 1367
Length:1,367
Mass (Da):136,111
Last modified:February 1, 1995 - v2
Checksum:i91C00E2DBD61AA9D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z38061 Genomic DNA. Translation: CAA86176.1.
M16164 Genomic DNA. Translation: AAA35014.1.
M16165 Genomic DNA. Translation: AAA35015.1.
X13857 Genomic DNA. Translation: CAA32069.1.
BK006942 Genomic DNA. Translation: DAA08566.1.
PIRiS48478.
RefSeqiNP_012284.3. NM_001179541.3.

Genome annotation databases

EnsemblFungiiYIR019C; YIR019C; YIR019C.
GeneIDi854836.
KEGGisce:YIR019C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z38061 Genomic DNA. Translation: CAA86176.1 .
M16164 Genomic DNA. Translation: AAA35014.1 .
M16165 Genomic DNA. Translation: AAA35015.1 .
X13857 Genomic DNA. Translation: CAA32069.1 .
BK006942 Genomic DNA. Translation: DAA08566.1 .
PIRi S48478.
RefSeqi NP_012284.3. NM_001179541.3.

3D structure databases

ProteinModelPortali P08640.
ModBasei Search...

Protein-protein interaction databases

BioGridi 35011. 78 interactions.
IntActi P08640. 2 interactions.
MINTi MINT-2782681.

Proteomic databases

PeptideAtlasi P08640.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YIR019C ; YIR019C ; YIR019C .
GeneIDi 854836.
KEGGi sce:YIR019C.

Organism-specific databases

CYGDi YIR019c.
SGDi S000001458. MUC1.

Phylogenomic databases

eggNOGi NOG12793.
GeneTreei ENSGT00730000112629.
KOi K01178.
OMAi ADQFTYV.
OrthoDBi EOG7S7SS8.

Enzyme and pathway databases

BioCyci YEAST:G3O-31439-MONOMER.

Miscellaneous databases

NextBioi 977713.
PROi P08640.

Gene expression databases

Genevestigatori P08640.

Family and domain databases

InterProi IPR018789. Uncharacterised_Flo11-rel_N.
[Graphical view ]
Pfami PF10182. Flo11. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Gene fusion is a possible mechanism underlying the evolution of STA1."
    Yamashita I., Nakamura M., Fukui S.
    J. Bacteriol. 169:2142-2149(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-242 AND 762-1331.
  4. "Similar short elements in the 5' regions of the STA2 and SGA genes from Saccharomyces cerevisiae."
    Pardo J.M., Ianez E., Zalacain M., Claros M.G., Jimenez A.
    FEBS Lett. 239:179-184(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31.
    Strain: SPX101-1C.
  5. "FLO11, a yeast gene related to the STA genes, encodes a novel cell surface flocculin."
    Lo W.S., Dranginis A.M.
    J. Bacteriol. 178:7144-7151(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "Muc1, a mucin-like protein that is regulated by Mss10, is critical for pseudohyphal differentiation in yeast."
    Lambrechts M.G., Bauer F.F., Marmur J., Pretorius I.S.
    Proc. Natl. Acad. Sci. U.S.A. 93:8419-8424(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Amino acid residues in the omega-minus region participate in cellular localization of yeast glycosylphosphatidylinositol-attached proteins."
    Hamada K., Terashima H., Arisawa M., Yabuki N., Kitada K.
    J. Bacteriol. 181:3886-3889(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "A Saccharomyces gene family involved in invasive growth, cell-cell adhesion, and mating."
    Guo B., Styles C.A., Feng Q., Fink G.R.
    Proc. Natl. Acad. Sci. U.S.A. 97:12158-12163(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  9. "Characteristics of Flo11-dependent flocculation in Saccharomyces cerevisiae."
    Bayly J.C., Douglas L.M., Pretorius I.S., Bauer F.F., Dranginis A.M.
    FEMS Yeast Res. 5:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Intragenic tandem repeats generate functional variability."
    Verstrepen K.J., Jansen A., Lewitter F., Fink G.R.
    Nat. Genet. 37:986-990(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: REPEATS.
  11. "Expression and characterization of the flocculin Flo11/Muc1, a Saccharomyces cerevisiae mannoprotein with homotypic properties of adhesion."
    Douglas L.M., Li L., Yang Y., Dranginis A.M.
    Eukaryot. Cell 6:2214-2221(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, GLYCOSYLATION.
  12. "Differential Flo8p-dependent regulation of FLO1 and FLO11 for cell-cell and cell-substrate adherence of S.cerevisiae S288c."
    Fichtner L., Schulze F., Braus G.H.
    Mol. Microbiol. 66:1276-1289(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: Sigma 1278B.

Entry informationi

Entry nameiMUC1_YEAST
AccessioniPrimary (citable) accession number: P08640
Secondary accession number(s): D6VVV0, P08068
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 1, 1995
Last modified: July 9, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3

Similar proteinsi