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P08640 (MUC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Flocculation protein FLO11

Short name=Flocculin-11
Alternative name(s):
Mucin-like protein 1
Gene names
Name:MUC1
Synonyms:FLO11, MAL5, S1, S2
Ordered Locus Names:YIR019C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1367 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca2+-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells. Activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose. Also involved in cell-substrate adhesion, haploid invasive growth, diploid pseudohyphae formation and biofilm (flor) development. Ref.5 Ref.6 Ref.8 Ref.9 Ref.11 Ref.12

Subcellular location

Secretedcell wall Probable. Membrane; Lipid-anchorGPI-anchor Potential Ref.5 Ref.7 Ref.8.

Domain

The number of the intragenic tandem repeats varies between different S.cerevisiae strains. There is a linear correlation between protein size and the extend of adhesion: the more repeats, the stronger the adhesion properties and the greater the fraction of flocculating cells By similarity.

Post-translational modification

Extensively O-mannosylated.

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer By similarity.

Sequence similarities

Belongs to the MUC1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 13461325Flocculation protein FLO11
PRO_0000019586
Propeptide1347 – 136721Removed in mature form Potential
PRO_0000019587

Regions

Repeat210 – 219101-1
Repeat220 – 229101-2
Repeat230 – 239101-3
Repeat240 – 249101-4
Repeat262 – 274132-1
Repeat275 – 287132-2
Repeat313 – 327153-1
Repeat328 – 342153-2
Repeat343 – 354124-1
Repeat355 – 369153-3
Repeat370 – 381124-2
Repeat382 – 393124-3
Repeat394 – 408153-4
Repeat409 – 420124-4
Repeat421 – 432124-5
Repeat433 – 444124-6
Repeat445 – 456124-7
Repeat457 – 471153-5
Repeat472 – 483124-8
Repeat484 – 498153-6
Repeat499 – 510124-9
Repeat511 – 525153-7
Repeat526 – 540153-8
Repeat541 – 552124-10
Repeat568 – 579124-11
Repeat580 – 594153-9
Repeat595 – 609153-10
Repeat610 – 625163-11
Repeat625 – 636124-12
Repeat637 – 651153-12
Repeat652 – 666153-13
Repeat667 – 681153-14
Repeat682 – 693124-13
Repeat694 – 705124-14
Repeat706 – 720153-15
Repeat721 – 735153-16
Repeat736 – 750153-17
Repeat751 – 762124-15
Repeat763 – 777153-18
Repeat778 – 792153-19
Repeat808 – 822153-21
Repeat838 – 852153-20
Repeat865 – 879153-22
Repeat937 – 968325-1
Repeat981 – 1012325-2
Repeat1088 – 1119325-3
Region210 – 249404 X 10 AA repeats, Ser/Thr-rich
Region262 – 287262 X 13 AA repeats, Thr-rich
Region313 – 85254022 X 15 AA approximate repeats, Ser-rich
Region343 – 76242015 X 12 AA repeats, Ser/Thr-rich
Region937 – 11191833 X 32 AA tandem repeats, Thr-rich

Amino acid modifications

Lipidation13461GPI-anchor amidated glycine Potential
Glycosylation8171N-linked (GlcNAc...) Potential
Glycosylation8741N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P08640 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 91C00E2DBD61AA9D

FASTA1,367136,111
        10         20         30         40         50         60 
MQRPFLLAYL VLSLLFNSAL GFPTALVPRG SSEGTSCNSI VNGCPNLDFN WHMDQQNIMQ 

        70         80         90        100        110        120 
YTLDVTSVSW VQDNTYQITI HVKGKENIDL KYLWSLKIIG VTGPKGTVQL YGYNENTYLI 

       130        140        150        160        170        180 
DNPTDFTATF EVYATQDVNS CQVWMPNFQI QFEYLQGSAA QYASSWQWGT TSFDLSTGCN 

       190        200        210        220        230        240 
NYDNQGHSQT DFPGFYWNID CDNNCGGTKS STTTSSTSES STTTSSTSES STTTSSTSES 

       250        260        270        280        290        300 
STTTSSTSES STSSSTTAPA TPTTTSCTKE KPTPPTTTSC TKEKPTPPHH DTTPCTKKKT 

       310        320        330        340        350        360 
TTSKTCTKKT TTPVPTPSSS TTESSSAPVP TPSSSTTESS SAPVTSSTTE SSSAPVPTPS 

       370        380        390        400        410        420 
SSTTESSSAP VTSSTTESSS APVTSSTTES SSAPVPTPSS STTESSSAPV TSSTTESSSA 

       430        440        450        460        470        480 
PVTSSTTESS SAPVTSSTTE SSSAPVTSST TESSSAPVPT PSSSTTESSS APVTSSTTES 

       490        500        510        520        530        540 
SSAPVPTPSS STTESSSAPV TSSTTESSSA PVPTPSSSTT ESSSAPAPTP SSSTTESSSA 

       550        560        570        580        590        600 
PVTSSTTESS SAPVPTPSSS TTESSSTPVT SSTTESSSAP VPTPSSSTTE SSSAPVPTPS 

       610        620        630        640        650        660 
SSTTESSSAP APTPSSSTTE SSSAPVTSST TESSSAPVPT PSSSTTESSS APVPTPSSST 

       670        680        690        700        710        720 
TESSSAPVPT PSSSTTESSS APVTSSTTES SSAPVTSSTT ESSSAPVPTP SSSTTESSSA 

       730        740        750        760        770        780 
PVPTPSSSTT ESSSAPVPTP SSSTTESSSA PVTSSTTESS SAPVPTPSSS TTESSSAPVP 

       790        800        810        820        830        840 
TPSSSTTESS SAPVPTPSSS TTESSVAPVP TPSSSSNITS SAPSSTPFSS STESSSVPVP 

       850        860        870        880        890        900 
TPSSSTTESS SAPVSSSTTE SSVAPVPTPS SSSNITSSAP SSIPFSSTTE SFSTGTTVTP 

       910        920        930        940        950        960 
SSSKYPGSQT ETSVSSTTET TIVPTKTTTS VTTPSTTTIT TTVCSTGTNS AGETTSGCSP 

       970        980        990       1000       1010       1020 
KTVTTTVPTT TTTSVTTSST TTITTTVCST GTNSAGETTS GCSPKTITTT VPCSTSPSET 

      1030       1040       1050       1060       1070       1080 
ASESTTTSPT TPVTTVVSTT VVTTEYSTST KPGGEITTTF VTKNIPTTYL TTIAPTPSVT 

      1090       1100       1110       1120       1130       1140 
TVTNFTPTTI TTTVCSTGTN SAGETTSGCS PKTVTTTVPC STGTGEYTTE ATTLVTTAVT 

      1150       1160       1170       1180       1190       1200 
TTVVTTESST GTNSAGKTTT GYTTKSVPTT YVTTLAPSAP VTPATNAVPT TITTTECSAA 

      1210       1220       1230       1240       1250       1260 
TNAAGETTSV CSAKTIVSSA SAGENTAPSA TTPVTTAIPT TVITTESSVG TNSAGETTTG 

      1270       1280       1290       1300       1310       1320 
YTTKSIPTTY ITTLIPGSNG AKNYETVATA TNPISIKTTS QLATTASASS VAPVVTSPSL 

      1330       1340       1350       1360 
TGPLQSASGS AVATYSVPSI SSTYQGAANI KVLGNFMWLL LALPVVF 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Gene fusion is a possible mechanism underlying the evolution of STA1."
Yamashita I., Nakamura M., Fukui S.
J. Bacteriol. 169:2142-2149(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-242 AND 762-1331.
[4]"Similar short elements in the 5' regions of the STA2 and SGA genes from Saccharomyces cerevisiae."
Pardo J.M., Ianez E., Zalacain M., Claros M.G., Jimenez A.
FEBS Lett. 239:179-184(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31.
Strain: SPX101-1C.
[5]"FLO11, a yeast gene related to the STA genes, encodes a novel cell surface flocculin."
Lo W.S., Dranginis A.M.
J. Bacteriol. 178:7144-7151(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]"Muc1, a mucin-like protein that is regulated by Mss10, is critical for pseudohyphal differentiation in yeast."
Lambrechts M.G., Bauer F.F., Marmur J., Pretorius I.S.
Proc. Natl. Acad. Sci. U.S.A. 93:8419-8424(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Amino acid residues in the omega-minus region participate in cellular localization of yeast glycosylphosphatidylinositol-attached proteins."
Hamada K., Terashima H., Arisawa M., Yabuki N., Kitada K.
J. Bacteriol. 181:3886-3889(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[8]"A Saccharomyces gene family involved in invasive growth, cell-cell adhesion, and mating."
Guo B., Styles C.A., Feng Q., Fink G.R.
Proc. Natl. Acad. Sci. U.S.A. 97:12158-12163(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[9]"Characteristics of Flo11-dependent flocculation in Saccharomyces cerevisiae."
Bayly J.C., Douglas L.M., Pretorius I.S., Bauer F.F., Dranginis A.M.
FEMS Yeast Res. 5:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Intragenic tandem repeats generate functional variability."
Verstrepen K.J., Jansen A., Lewitter F., Fink G.R.
Nat. Genet. 37:986-990(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: REPEATS.
[11]"Expression and characterization of the flocculin Flo11/Muc1, a Saccharomyces cerevisiae mannoprotein with homotypic properties of adhesion."
Douglas L.M., Li L., Yang Y., Dranginis A.M.
Eukaryot. Cell 6:2214-2221(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, GLYCOSYLATION.
[12]"Differential Flo8p-dependent regulation of FLO1 and FLO11 for cell-cell and cell-substrate adherence of S.cerevisiae S288c."
Fichtner L., Schulze F., Braus G.H.
Mol. Microbiol. 66:1276-1289(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: Sigma 1278B.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z38061 Genomic DNA. Translation: CAA86176.1.
M16164 Genomic DNA. Translation: AAA35014.1.
M16165 Genomic DNA. Translation: AAA35015.1.
X13857 Genomic DNA. Translation: CAA32069.1.
BK006942 Genomic DNA. Translation: DAA08566.1.
PIRS48478.
RefSeqNP_012284.3. NM_001179541.3.

3D structure databases

ProteinModelPortalP08640.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35011. 78 interactions.
IntActP08640. 2 interactions.
MINTMINT-2782681.

Proteomic databases

PeptideAtlasP08640.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIR019C; YIR019C; YIR019C.
GeneID854836.
KEGGsce:YIR019C.

Organism-specific databases

CYGDYIR019c.
SGDS000001458. MUC1.

Phylogenomic databases

eggNOGNOG12793.
GeneTreeENSGT00730000112629.
KOK01178.
OMAADQFTYV.
OrthoDBEOG7S7SS8.

Enzyme and pathway databases

BioCycYEAST:G3O-31439-MONOMER.

Gene expression databases

GenevestigatorP08640.

Family and domain databases

InterProIPR018789. Uncharacterised_Flo11-rel_N.
[Graphical view]
PfamPF10182. Flo11. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977713.
PROP08640.

Entry information

Entry nameMUC1_YEAST
AccessionPrimary (citable) accession number: P08640
Secondary accession number(s): D6VVV0, P08068
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 1, 1995
Last modified: July 9, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families