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Protein

Regulatory protein LEU3

Gene

LEU3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Factor for control of RNA levels of a group of leucine-specific genes.

Miscellaneous

Present with 125 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi37 – 67Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd BLAST31

GO - Molecular functioni

GO - Biological processi

  • leucine biosynthetic process Source: UniProtKB-KW
  • negative regulation of transcription by RNA polymerase II Source: SGD
  • positive regulation of leucine biosynthetic process Source: SGD
  • positive regulation of transcription by RNA polymerase II Source: SGD

Keywordsi

Molecular functionActivator, DNA-binding
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32504-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein LEU3
Gene namesi
Name:LEU3
Ordered Locus Names:YLR451W
ORF Names:L9324.1
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR451W
SGDiS000004443 LEU3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001149541 – 886Regulatory protein LEU3Add BLAST886

Proteomic databases

MaxQBiP08638
PaxDbiP08638
PRIDEiP08638

PTM databases

iPTMnetiP08638

Interactioni

Protein-protein interaction databases

BioGridi31709, 69 interactors
DIPiDIP-2400N
IntActiP08638, 2 interactors
MINTiP08638
STRINGi4932.YLR451W

Structurei

Secondary structure

1886
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi38 – 42Combined sources5
Helixi50 – 52Combined sources3
Helixi58 – 62Combined sources5
Helixi78 – 97Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ER8X-ray2.85A/B/C/D32-103[»]
2EREX-ray3.00A/B32-103[»]
2ERGX-ray3.15A/B32-103[»]
ProteinModelPortaliP08638
SMRiP08638
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08638

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi874 – 8829aaTAD9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi678 – 697Asp/Glu-rich (acidic)Add BLAST20

Domaini

the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.1 Publication

Phylogenomic databases

GeneTreeiENSGT00530000068718
HOGENOMiHOG000074528
InParanoidiP08638
KOiK09242
OMAiWAVCNIV
OrthoDBiEOG092C0IFA

Family and domain databases

CDDicd00067 GAL4, 1 hit
Gene3Di4.10.240.10, 1 hit
InterProiView protein in InterPro
IPR001138 Zn2-C6_fun-type_DNA-bd
IPR036864 Zn2-C6_fun-type_DNA-bd_sf
PfamiView protein in Pfam
PF00172 Zn_clus, 1 hit
SMARTiView protein in SMART
SM00066 GAL4, 1 hit
SUPFAMiSSF57701 SSF57701, 1 hit
PROSITEiView protein in PROSITE
PS00463 ZN2_CY6_FUNGAL_1, 1 hit
PS50048 ZN2_CY6_FUNGAL_2, 1 hit

Sequencei

Sequence statusi: Complete.

P08638-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGRSDFVAT SQSGSEMSHS ETRNRTGMNA RKRKFACVEC RQQKSKCDAH
60 70 80 90 100
ERAPEPCTKC AKKNVPCILK RDFRRTYKRA RNEAIEKRFK ELTRTLTNLT
110 120 130 140 150
SDEILKKIEE EQEIVLDNSN FTKEKVKQLR KSAFETTEIE PRSYKTLRGE
160 170 180 190 200
PISYSTNRRH TDSSPLTLLS SSTNFDPVHS TNVMTDDQLK CLPKSLGDVY
210 220 230 240 250
LSSSDIAELF QEFATKYHQF LPVVDLSKGA ERIYHLSPCL FWVILLIGLR
260 270 280 290 300
RKFGATDLMT RLSVLVKSVL SEITISPIIR YTPSDKDEPV LNVASVYSVQ
310 320 330 340 350
AFLLYTFWPP LTSSLSADTS WNTIGTAMFQ ALRVGLNCAG FSKEYASANS
360 370 380 390 400
ELVNEQIRTW ICCNVVSQTV ASSFGFPAYV SFDYLVISSI RVPNSKSQVD
410 420 430 440 450
IPNELRQMAQ IARFENQIVN TMNSTPASVT GMVSQEEKQP LLHVLNQQLS
460 470 480 490 500
QLEISLEENN LDDIRKFLLL VAKVHLLTYY FTDVTSQSAG KSNGNIYEGS
510 520 530 540 550
YSIMELDTSF ETKRGLVKVY NAAVNFLIHA NSMWEHDPTI IKYFPGLFVL
560 570 580 590 600
NIWQSACIIS KLIHSSLHSM LDVNSGKKAY NNAISLTFNA SVLKYDMAYR
610 620 630 640 650
SSGIMRSIWS LFANMYDAWK NDQKEGGGRL NNDFNLGITI KSRMSVNVFF
660 670 680 690 700
DCLYILKEKC GMAKLERETK VSTAYNVDEE EEEDEDEEGE EEEEEEELSS
710 720 730 740 750
KVPENMDSQQ LRTRKFTNVR HPEKKARKII ETIPLDPNPI NAGSTSSGSS
760 770 780 790 800
LTTPNSQVAN TISYRGILNK MSPREQLNHA NLDSSVSTDI KDTEAVNEPL
810 820 830 840 850
PIGRNAEHPA NQPPLSITQM QENTLPATQA NSSLLETYPI VQSNPVTTTI
860 870 880
KESPNSIMAG WDNWESDMVW RDVDILMNEF AFNPKV
Length:886
Mass (Da):100,153
Last modified:August 1, 1988 - v1
Checksum:iC5F8EDCD326B1EBB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti504M → I in AAA34741 (PubMed:2823102).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00360 Genomic DNA Translation: CAA68438.1
M17222 Genomic DNA Translation: AAA34741.1
U22382 Genomic DNA Translation: AAB67526.1
BK006945 Genomic DNA Translation: DAA09751.1
PIRiS00638 RGBYL3
RefSeqiNP_013556.3, NM_001182339.3

Genome annotation databases

EnsemblFungiiYLR451W; YLR451W; YLR451W
GeneIDi851172
KEGGisce:YLR451W

Similar proteinsi

Entry informationi

Entry nameiLEUR_YEAST
AccessioniPrimary (citable) accession number: P08638
Secondary accession number(s): D6VZ85
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: March 28, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

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