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Protein

Regulatory protein LEU3

Gene

LEU3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Factor for control of RNA levels of a group of leucine-specific genes.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi37 – 67Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd BLAST31

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32504-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein LEU3
Gene namesi
Name:LEU3
Ordered Locus Names:YLR451W
ORF Names:L9324.1
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR451W.
SGDiS000004443. LEU3.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001149541 – 886Regulatory protein LEU3Add BLAST886

Proteomic databases

MaxQBiP08638.
PRIDEiP08638.

PTM databases

iPTMnetiP08638.

Interactioni

Protein-protein interaction databases

BioGridi31709. 48 interactors.
DIPiDIP-2400N.
IntActiP08638. 3 interactors.
MINTiMINT-423692.

Structurei

Secondary structure

1886
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi38 – 42Combined sources5
Helixi50 – 52Combined sources3
Helixi58 – 62Combined sources5
Helixi78 – 97Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ER8X-ray2.85A/B/C/D32-103[»]
2EREX-ray3.00A/B32-103[»]
2ERGX-ray3.15A/B32-103[»]
ProteinModelPortaliP08638.
SMRiP08638.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08638.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi874 – 8829aaTAD9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi678 – 697Asp/Glu-rich (acidic)Add BLAST20

Domaini

the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.1 Publication

Sequence similaritiesi

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000068718.
HOGENOMiHOG000074528.
InParanoidiP08638.
KOiK09242.
OMAiFACVECR.
OrthoDBiEOG092C0IFA.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08638-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGRSDFVAT SQSGSEMSHS ETRNRTGMNA RKRKFACVEC RQQKSKCDAH
60 70 80 90 100
ERAPEPCTKC AKKNVPCILK RDFRRTYKRA RNEAIEKRFK ELTRTLTNLT
110 120 130 140 150
SDEILKKIEE EQEIVLDNSN FTKEKVKQLR KSAFETTEIE PRSYKTLRGE
160 170 180 190 200
PISYSTNRRH TDSSPLTLLS SSTNFDPVHS TNVMTDDQLK CLPKSLGDVY
210 220 230 240 250
LSSSDIAELF QEFATKYHQF LPVVDLSKGA ERIYHLSPCL FWVILLIGLR
260 270 280 290 300
RKFGATDLMT RLSVLVKSVL SEITISPIIR YTPSDKDEPV LNVASVYSVQ
310 320 330 340 350
AFLLYTFWPP LTSSLSADTS WNTIGTAMFQ ALRVGLNCAG FSKEYASANS
360 370 380 390 400
ELVNEQIRTW ICCNVVSQTV ASSFGFPAYV SFDYLVISSI RVPNSKSQVD
410 420 430 440 450
IPNELRQMAQ IARFENQIVN TMNSTPASVT GMVSQEEKQP LLHVLNQQLS
460 470 480 490 500
QLEISLEENN LDDIRKFLLL VAKVHLLTYY FTDVTSQSAG KSNGNIYEGS
510 520 530 540 550
YSIMELDTSF ETKRGLVKVY NAAVNFLIHA NSMWEHDPTI IKYFPGLFVL
560 570 580 590 600
NIWQSACIIS KLIHSSLHSM LDVNSGKKAY NNAISLTFNA SVLKYDMAYR
610 620 630 640 650
SSGIMRSIWS LFANMYDAWK NDQKEGGGRL NNDFNLGITI KSRMSVNVFF
660 670 680 690 700
DCLYILKEKC GMAKLERETK VSTAYNVDEE EEEDEDEEGE EEEEEEELSS
710 720 730 740 750
KVPENMDSQQ LRTRKFTNVR HPEKKARKII ETIPLDPNPI NAGSTSSGSS
760 770 780 790 800
LTTPNSQVAN TISYRGILNK MSPREQLNHA NLDSSVSTDI KDTEAVNEPL
810 820 830 840 850
PIGRNAEHPA NQPPLSITQM QENTLPATQA NSSLLETYPI VQSNPVTTTI
860 870 880
KESPNSIMAG WDNWESDMVW RDVDILMNEF AFNPKV
Length:886
Mass (Da):100,153
Last modified:August 1, 1988 - v1
Checksum:iC5F8EDCD326B1EBB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti504M → I in AAA34741 (PubMed:2823102).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00360 Genomic DNA. Translation: CAA68438.1.
M17222 Genomic DNA. Translation: AAA34741.1.
U22382 Genomic DNA. Translation: AAB67526.1.
BK006945 Genomic DNA. Translation: DAA09751.1.
PIRiS00638. RGBYL3.
RefSeqiNP_013556.3. NM_001182339.3.

Genome annotation databases

EnsemblFungiiYLR451W; YLR451W; YLR451W.
GeneIDi851172.
KEGGisce:YLR451W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00360 Genomic DNA. Translation: CAA68438.1.
M17222 Genomic DNA. Translation: AAA34741.1.
U22382 Genomic DNA. Translation: AAB67526.1.
BK006945 Genomic DNA. Translation: DAA09751.1.
PIRiS00638. RGBYL3.
RefSeqiNP_013556.3. NM_001182339.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ER8X-ray2.85A/B/C/D32-103[»]
2EREX-ray3.00A/B32-103[»]
2ERGX-ray3.15A/B32-103[»]
ProteinModelPortaliP08638.
SMRiP08638.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31709. 48 interactors.
DIPiDIP-2400N.
IntActiP08638. 3 interactors.
MINTiMINT-423692.

PTM databases

iPTMnetiP08638.

Proteomic databases

MaxQBiP08638.
PRIDEiP08638.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR451W; YLR451W; YLR451W.
GeneIDi851172.
KEGGisce:YLR451W.

Organism-specific databases

EuPathDBiFungiDB:YLR451W.
SGDiS000004443. LEU3.

Phylogenomic databases

GeneTreeiENSGT00530000068718.
HOGENOMiHOG000074528.
InParanoidiP08638.
KOiK09242.
OMAiFACVECR.
OrthoDBiEOG092C0IFA.

Enzyme and pathway databases

BioCyciYEAST:G3O-32504-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP08638.
PROiP08638.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEUR_YEAST
AccessioniPrimary (citable) accession number: P08638
Secondary accession number(s): D6VZ85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 2, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 125 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.