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Protein

Tyrosine-protein kinase Btk29A

Gene

Btk29A

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Required for proper ring canal development. Also required for the development of male genitalia and for adult survival.2 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi195ZincBy similarity1
Metal bindingi206ZincBy similarity1
Metal bindingi207ZincBy similarity1
Metal bindingi217ZincBy similarity1
Binding sitei554ATPPROSITE-ProRule annotation1
Active sitei647Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri187 – 223Btk-typePROSITE-ProRule annotationAdd BLAST37
Nucleotide bindingi532 – 540ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: FlyBase
  • cellularization Source: FlyBase
  • courtship behavior Source: FlyBase
  • determination of adult lifespan Source: UniProtKB
  • DNA endoreduplication Source: FlyBase
  • dorsal closure Source: FlyBase
  • female germline ring canal formation Source: UniProtKB
  • head involution Source: FlyBase
  • imaginal disc-derived male genitalia development Source: UniProtKB
  • innate immune response Source: GO_Central
  • JNK cascade Source: FlyBase
  • oocyte karyosome formation Source: FlyBase
  • oogenesis Source: FlyBase
  • open tracheal system development Source: FlyBase
  • ovarian fusome organization Source: FlyBase
  • ovarian nurse cell to oocyte transport Source: FlyBase
  • peptidyl-tyrosine autophosphorylation Source: GO_Central
  • peptidyl-tyrosine phosphorylation Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • regulation of actin filament polymerization Source: FlyBase
  • regulation of cell proliferation Source: GO_Central
  • regulation of imaginal disc-derived wing size Source: FlyBase
  • salivary gland morphogenesis Source: FlyBase
  • sensitization Source: FlyBase
  • spiracle morphogenesis, open tracheal system Source: FlyBase
  • transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central

Keywordsi

Molecular functionKinase, Transferase, Tyrosine-protein kinase
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-111465 Apoptotic cleavage of cellular proteins
R-DME-1660499 Synthesis of PIPs at the plasma membrane
R-DME-2029482 Regulation of actin dynamics for phagocytic cup formation
R-DME-512988 Interleukin-3, 5 and GM-CSF signaling
R-DME-5663213 RHO GTPases Activate WASPs and WAVEs
SignaLinkiP08630

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase Btk29A (EC:2.7.10.2)
Alternative name(s):
Dsrc28C
Dsrc29a
Gene namesi
Name:Btk29A
Synonyms:Src2, Src29A, Tec29
ORF Names:CG8049
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0003502 Btk29A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Flies exhibit shortened copulatory duration (due to incomplete fusion of the left and right halves of the apodeme that holds the penis during copulation) and reduced adult-stage life span.1 Publication

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000880681 – 786Tyrosine-protein kinase Btk29AAdd BLAST786

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei677Phosphotyrosine; by autocatalysisBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP08630
PRIDEiP08630

PTM databases

iPTMnetiP08630

Expressioni

Tissue specificityi

Ring canals in the egg chambers and imaginal disks of third-instar larvae.3 Publications

Developmental stagei

Expressed both maternally and zygotically. Predominantly in early to middle embryogenesis, in larvae and adult females.2 Publications

Gene expression databases

BgeeiFBgn0003502
ExpressionAtlasiP08630 baseline and differential
GenevisibleiP08630 DM

Interactioni

Protein-protein interaction databases

BioGridi60259, 23 interactors
DIPiDIP-23748N
IntActiP08630, 1 interactor
STRINGi7227.FBpp0079237

Structurei

3D structure databases

ProteinModelPortaliP08630
SMRiP08630
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 184PHPROSITE-ProRule annotationAdd BLAST144
Domaini342 – 402SH3PROSITE-ProRule annotationAdd BLAST61
Domaini410 – 503SH2PROSITE-ProRule annotationAdd BLAST94
Domaini526 – 779Protein kinasePROSITE-ProRule annotationAdd BLAST254

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi14 – 27Ser-richAdd BLAST14
Compositional biasi231 – 264Ser-richAdd BLAST34
Compositional biasi263 – 289Gly-richAdd BLAST27

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri187 – 223Btk-typePROSITE-ProRule annotationAdd BLAST37

Keywords - Domaini

SH2 domain, SH3 domain, Zinc-finger

Phylogenomic databases

eggNOGiKOG0197 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00760000119011
InParanoidiP08630
KOiK07364
OrthoDBiEOG091G0D46
PhylomeDBiP08630

Family and domain databases

Gene3Di3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR001849 PH_domain
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
IPR001562 Znf_Btk_motif
PfamiView protein in Pfam
PF00779 BTK, 1 hit
PF00169 PH, 1 hit
PF07714 Pkinase_Tyr, 1 hit
PF00017 SH2, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00401 SH2DOMAIN
PR00109 TYRKINASE
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00252 SH2, 1 hit
SM00326 SH3, 1 hit
SM00219 TyrKc, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
SSF55550 SSF55550, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit
PS50001 SH2, 1 hit
PS50002 SH3, 1 hit
PS51113 ZF_BTK, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: P08630-1) [UniParc]FASTAAdd to basket
Also known as: D, E, Type 2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMGTKHRNSH VNGSIKSSSS LRSSSKSFQA KMDLMSERLY DVVKSGSMVK
60 70 80 90 100
RAQNKKRFTP VNYKHRWFEL TKRTFSYFDV ENVERRRERG RIHLKGVRLV
110 120 130 140 150
EEATVSGEGG DPFAPDGYPF QVGYCEISAS ANSHQLENGN GGGSGVGIEG
160 170 180 190 200
QQSGRAVPQY TLYVIANSEK ERSEWIRAIR QVCEDSNTPK SYRYHPGLWS
210 220 230 240 250
GKKWSCCKGL SRTTFGCRAA AHWREANNNP SNGSSPAQNS TRSISPNSST
260 270 280 290 300
TNSQFSLQHN SSGSLGGGVG GGLGGGGSLG LGGGGGGGGS CTPTSLQPQS
310 320 330 340 350
SLTTFKQSPT LLNGNGTLLD ANMPGGIPTP GTPNSKAKDN SHFVKLVVAL
360 370 380 390 400
YPFKAIEGGD LSLEKNAEYE VIDDSQEHWW KVKDALGNVG YIPSNYVKPK
410 420 430 440 450
ALLGLERYEW YVGDMSRQRA ESLLKQGDKE GCFVVRKSST KGLYTLSLHT
460 470 480 490 500
KVPQSHVKHY HIKQNARCEY YLSEKHCCET IPDLINYHRH NSGGLACRLK
510 520 530 540 550
SSPCDRPVPP TAGLSHDKWE IHPMELMLME ELGSGQFGVV RRGKWRGSID
560 570 580 590 600
TAVKMMKEGT MSEDDFIEEA KVMTKLQHPN LVQLYGVCSK HRPIYIVTEY
610 620 630 640 650
MKHGSLLNYL RRHEKTLIGN MGLLLDMCIQ VSKGMTYLER HNYIHRDLAA
660 670 680 690 700
RNCLVGSENV VKVADFGLAR YVLDDQYTSS GGTKFPIKWA PPEVLNYTRF
710 720 730 740 750
SSKSDVWAYG VLMWEIFTCG KMPYGRLKNT EVVERVQRGI ILEKPKSCAK
760 770 780
EIYDVMKLCW SHGPEERPAF RVLMDQLALV AQTLTD
Length:786
Mass (Da):87,392
Last modified:November 25, 2002 - v2
Checksum:iE2BCE122E6AAA0AA
GO
Isoform A (identifier: P08630-2) [UniParc]FASTAAdd to basket
Also known as: C, F, Type 1

The sequence of this isoform differs from the canonical sequence as follows:
     1-183: Missing.
     184-231: EDSNTPKSYR...HWREANNNPS → MIPCVSLAET...SLTSSKTKEG

Show »
Length:603
Mass (Da):66,750
Checksum:i6FDEE9B1F2C230AD
GO

Sequence cautioni

The sequence AAA28912 differs from that shown. Reason: Frameshift at positions 62, 215, 218, 220 and 401.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti243S → A in AAA28912 (PubMed:3110602).Curated1
Sequence conflicti317T → N in AAA28912 (PubMed:3110602).Curated1
Sequence conflicti317T → N in BAA24063 (PubMed:10330180).Curated1
Sequence conflicti317T → N in BAA24064 (PubMed:10330180).Curated1
Sequence conflicti352 – 353PF → LG in AAA28912 (PubMed:3110602).Curated2
Sequence conflicti363L → VG in AAA28912 (PubMed:3110602).Curated1
Sequence conflicti398 – 400KPK → QAE in AAA28912 (PubMed:3110602).Curated3
Sequence conflicti414D → Y in AAA28912 (PubMed:3110602).Curated1
Sequence conflicti524 – 525ME → IQ in AAA28912 (PubMed:3110602).Curated2
Sequence conflicti589S → T in AAA28912 (PubMed:3110602).Curated1
Sequence conflicti589S → T in CAA26170 (PubMed:9660966).Curated1
Sequence conflicti657S → F in CAA26170 (PubMed:9660966).Curated1
Sequence conflicti681 – 684GGTK → AEPS in CAA26170 (PubMed:9660966).Curated4

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0049641 – 183Missing in isoform A. 5 PublicationsAdd BLAST183
Alternative sequenceiVSP_004965184 – 231EDSNT…NNNPS → MIPCVSLAETSVIGNMKERV KEMKVFGCRLNFWNHIGHSL TSSKTKEG in isoform A. 5 PublicationsAdd BLAST48

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16599 mRNA Translation: AAA28912.1 Frameshift.
AB009840 mRNA Translation: BAA24063.1
AB009841 mRNA Translation: BAA24064.1
AE014134 Genomic DNA Translation: AAF52631.3
AE014134 Genomic DNA Translation: AAF52632.2
AE014134 Genomic DNA Translation: AAN11161.1
AE014134 Genomic DNA Translation: AAN11162.1
AE014134 Genomic DNA Translation: ABV53648.1
AE014134 Genomic DNA Translation: ABV53649.1
AY069457 mRNA Translation: AAL39602.1
AY128441 mRNA Translation: AAM75034.1
BT011183 mRNA Translation: AAR88544.1
AF044337 mRNA Translation: AAB99858.1
X02305 Genomic DNA Translation: CAA26170.1
PIRiA23051
A27807 TVFFDS
RefSeqiNP_001097120.1, NM_001103650.2 [P08630-2]
NP_001097121.1, NM_001103651.3 [P08630-1]
NP_476745.1, NM_057397.6 [P08630-1]
NP_476746.1, NM_057398.4 [P08630-2]
NP_723369.1, NM_164804.2 [P08630-1]
NP_723370.1, NM_164805.2 [P08630-2]
UniGeneiDm.7306

Genome annotation databases

EnsemblMetazoaiFBtr0079615; FBpp0079235; FBgn0003502 [P08630-2]
FBtr0079616; FBpp0079236; FBgn0003502 [P08630-1]
FBtr0079617; FBpp0079237; FBgn0003502 [P08630-1]
FBtr0079618; FBpp0079238; FBgn0003502 [P08630-2]
FBtr0112835; FBpp0111748; FBgn0003502 [P08630-2]
FBtr0112836; FBpp0111749; FBgn0003502 [P08630-1]
GeneIDi34132
KEGGidme:Dmel_CG8049

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiBTKL_DROME
AccessioniPrimary (citable) accession number: P08630
Secondary accession number(s): A4V0F3
, A4V0F4, O45032, O76132, O76133, P11361, Q6NNV0, Q8T0A0, Q9VLQ2, Q9VLQ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 25, 2002
Last modified: May 23, 2018
This is version 205 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

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