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Protein

Tyrosine-protein kinase Btk29A

Gene

Btk29A

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Required for proper ring canal development. Also required for the development of male genitalia and for adult survival.2 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi195ZincBy similarity1
Metal bindingi206ZincBy similarity1
Metal bindingi207ZincBy similarity1
Metal bindingi217ZincBy similarity1
Binding sitei554ATPPROSITE-ProRule annotation1
Active sitei647Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri187 – 223Btk-typePROSITE-ProRule annotationAdd BLAST37
Nucleotide bindingi532 – 540ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: FlyBase
  • cellularization Source: FlyBase
  • courtship behavior Source: FlyBase
  • determination of adult lifespan Source: UniProtKB
  • DNA endoreduplication Source: FlyBase
  • dorsal closure Source: FlyBase
  • female germline ring canal formation Source: UniProtKB
  • head involution Source: FlyBase
  • imaginal disc-derived male genitalia development Source: UniProtKB
  • imaginal disc-derived wing morphogenesis Source: FlyBase
  • innate immune response Source: GO_Central
  • JNK cascade Source: FlyBase
  • karyosome formation Source: FlyBase
  • oogenesis Source: FlyBase
  • open tracheal system development Source: FlyBase
  • ovarian fusome organization Source: FlyBase
  • ovarian nurse cell to oocyte transport Source: FlyBase
  • peptidyl-tyrosine autophosphorylation Source: GO_Central
  • peptidyl-tyrosine phosphorylation Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • regulation of actin filament polymerization Source: FlyBase
  • regulation of cell proliferation Source: GO_Central
  • regulation of imaginal disc-derived wing size Source: FlyBase
  • salivary gland morphogenesis Source: FlyBase
  • sensitization Source: FlyBase
  • spiracle morphogenesis, open tracheal system Source: FlyBase
  • transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-111465. Apoptotic cleavage of cellular proteins.
R-DME-1660499. Synthesis of PIPs at the plasma membrane.
R-DME-512988. Interleukin-3, 5 and GM-CSF signaling.
SignaLinkiP08630.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase Btk29A (EC:2.7.10.2)
Alternative name(s):
Dsrc28C
Dsrc29a
Gene namesi
Name:Btk29A
Synonyms:Src2, Src29A, Tec29
ORF Names:CG8049
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0003502. Btk29A.

Subcellular locationi


    Note: Ring canals.

GO - Cellular componenti

  • apical part of cell Source: FlyBase
  • cell periphery Source: FlyBase
  • cytoplasm Source: FlyBase
  • extrinsic component of cytoplasmic side of plasma membrane Source: GO_Central
  • germline ring canal Source: UniProtKB
  • plasma membrane Source: FlyBase
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Flies exhibit shortened copulatory duration (due to incomplete fusion of the left and right halves of the apodeme that holds the penis during copulation) and reduced adult-stage life span.1 Publication

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000880681 – 786Tyrosine-protein kinase Btk29AAdd BLAST786

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei677Phosphotyrosine; by autocatalysisBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP08630.
PRIDEiP08630.

PTM databases

iPTMnetiP08630.

Expressioni

Tissue specificityi

Ring canals in the egg chambers and imaginal disks of third-instar larvae.3 Publications

Developmental stagei

Expressed both maternally and zygotically. Predominantly in early to middle embryogenesis, in larvae and adult females.2 Publications

Gene expression databases

BgeeiFBgn0003502.
ExpressionAtlasiP08630. baseline.
GenevisibleiP08630. DM.

Interactioni

Protein-protein interaction databases

BioGridi60259. 13 interactors.
DIPiDIP-23748N.
IntActiP08630. 1 interactor.
MINTiMINT-896102.
STRINGi7227.FBpp0079237.

Structurei

3D structure databases

ProteinModelPortaliP08630.
SMRiP08630.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 184PHPROSITE-ProRule annotationAdd BLAST144
Domaini342 – 402SH3PROSITE-ProRule annotationAdd BLAST61
Domaini410 – 503SH2PROSITE-ProRule annotationAdd BLAST94
Domaini526 – 779Protein kinasePROSITE-ProRule annotationAdd BLAST254

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi14 – 27Ser-richAdd BLAST14
Compositional biasi231 – 264Ser-richAdd BLAST34
Compositional biasi263 – 289Gly-richAdd BLAST27

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.PROSITE-ProRule annotation
Contains 1 Btk-type zinc finger.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri187 – 223Btk-typePROSITE-ProRule annotationAdd BLAST37

Keywords - Domaini

SH2 domain, SH3 domain, Zinc-finger

Phylogenomic databases

eggNOGiKOG0197. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000119011.
InParanoidiP08630.
KOiK07364.
OMAiRTQRGLF.
OrthoDBiEOG091G0D46.
PhylomeDBiP08630.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR001562. Znf_Btk_motif.
[Graphical view]
PfamiPF00779. BTK. 1 hit.
PF00169. PH. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00233. PH. 1 hit.
SM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 2 hits.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
PS51113. ZF_BTK. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: P08630-1) [UniParc]FASTAAdd to basket
Also known as: D, E, Type 2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMGTKHRNSH VNGSIKSSSS LRSSSKSFQA KMDLMSERLY DVVKSGSMVK
60 70 80 90 100
RAQNKKRFTP VNYKHRWFEL TKRTFSYFDV ENVERRRERG RIHLKGVRLV
110 120 130 140 150
EEATVSGEGG DPFAPDGYPF QVGYCEISAS ANSHQLENGN GGGSGVGIEG
160 170 180 190 200
QQSGRAVPQY TLYVIANSEK ERSEWIRAIR QVCEDSNTPK SYRYHPGLWS
210 220 230 240 250
GKKWSCCKGL SRTTFGCRAA AHWREANNNP SNGSSPAQNS TRSISPNSST
260 270 280 290 300
TNSQFSLQHN SSGSLGGGVG GGLGGGGSLG LGGGGGGGGS CTPTSLQPQS
310 320 330 340 350
SLTTFKQSPT LLNGNGTLLD ANMPGGIPTP GTPNSKAKDN SHFVKLVVAL
360 370 380 390 400
YPFKAIEGGD LSLEKNAEYE VIDDSQEHWW KVKDALGNVG YIPSNYVKPK
410 420 430 440 450
ALLGLERYEW YVGDMSRQRA ESLLKQGDKE GCFVVRKSST KGLYTLSLHT
460 470 480 490 500
KVPQSHVKHY HIKQNARCEY YLSEKHCCET IPDLINYHRH NSGGLACRLK
510 520 530 540 550
SSPCDRPVPP TAGLSHDKWE IHPMELMLME ELGSGQFGVV RRGKWRGSID
560 570 580 590 600
TAVKMMKEGT MSEDDFIEEA KVMTKLQHPN LVQLYGVCSK HRPIYIVTEY
610 620 630 640 650
MKHGSLLNYL RRHEKTLIGN MGLLLDMCIQ VSKGMTYLER HNYIHRDLAA
660 670 680 690 700
RNCLVGSENV VKVADFGLAR YVLDDQYTSS GGTKFPIKWA PPEVLNYTRF
710 720 730 740 750
SSKSDVWAYG VLMWEIFTCG KMPYGRLKNT EVVERVQRGI ILEKPKSCAK
760 770 780
EIYDVMKLCW SHGPEERPAF RVLMDQLALV AQTLTD
Length:786
Mass (Da):87,392
Last modified:November 25, 2002 - v2
Checksum:iE2BCE122E6AAA0AA
GO
Isoform A (identifier: P08630-2) [UniParc]FASTAAdd to basket
Also known as: C, F, Type 1

The sequence of this isoform differs from the canonical sequence as follows:
     1-183: Missing.
     184-231: EDSNTPKSYR...HWREANNNPS → MIPCVSLAET...SLTSSKTKEG

Show »
Length:603
Mass (Da):66,750
Checksum:i6FDEE9B1F2C230AD
GO

Sequence cautioni

The sequence AAA28912 differs from that shown. Reason: Frameshift at positions 62, 215, 218, 220 and 401.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti243S → A in AAA28912 (PubMed:3110602).Curated1
Sequence conflicti317T → N in AAA28912 (PubMed:3110602).Curated1
Sequence conflicti317T → N in BAA24063 (PubMed:10330180).Curated1
Sequence conflicti317T → N in BAA24064 (PubMed:10330180).Curated1
Sequence conflicti352 – 353PF → LG in AAA28912 (PubMed:3110602).Curated2
Sequence conflicti363L → VG in AAA28912 (PubMed:3110602).Curated1
Sequence conflicti398 – 400KPK → QAE in AAA28912 (PubMed:3110602).Curated3
Sequence conflicti414D → Y in AAA28912 (PubMed:3110602).Curated1
Sequence conflicti524 – 525ME → IQ in AAA28912 (PubMed:3110602).Curated2
Sequence conflicti589S → T in AAA28912 (PubMed:3110602).Curated1
Sequence conflicti589S → T in CAA26170 (PubMed:9660966).Curated1
Sequence conflicti657S → F in CAA26170 (PubMed:9660966).Curated1
Sequence conflicti681 – 684GGTK → AEPS in CAA26170 (PubMed:9660966).Curated4

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0049641 – 183Missing in isoform A. 5 PublicationsAdd BLAST183
Alternative sequenceiVSP_004965184 – 231EDSNT…NNNPS → MIPCVSLAETSVIGNMKERV KEMKVFGCRLNFWNHIGHSL TSSKTKEG in isoform A. 5 PublicationsAdd BLAST48

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16599 mRNA. Translation: AAA28912.1. Frameshift.
AB009840 mRNA. Translation: BAA24063.1.
AB009841 mRNA. Translation: BAA24064.1.
AE014134 Genomic DNA. Translation: AAF52631.3.
AE014134 Genomic DNA. Translation: AAF52632.2.
AE014134 Genomic DNA. Translation: AAN11161.1.
AE014134 Genomic DNA. Translation: AAN11162.1.
AE014134 Genomic DNA. Translation: ABV53648.1.
AE014134 Genomic DNA. Translation: ABV53649.1.
AY069457 mRNA. Translation: AAL39602.1.
AY128441 mRNA. Translation: AAM75034.1.
BT011183 mRNA. Translation: AAR88544.1.
AF044337 mRNA. Translation: AAB99858.1.
X02305 Genomic DNA. Translation: CAA26170.1.
PIRiA23051.
A27807. TVFFDS.
RefSeqiNP_001097120.1. NM_001103650.2. [P08630-2]
NP_001097121.1. NM_001103651.3. [P08630-1]
NP_476745.1. NM_057397.6. [P08630-1]
NP_476746.1. NM_057398.4. [P08630-2]
NP_723369.1. NM_164804.2. [P08630-1]
NP_723370.1. NM_164805.2. [P08630-2]
UniGeneiDm.7306.

Genome annotation databases

EnsemblMetazoaiFBtr0079616; FBpp0079236; FBgn0003502. [P08630-1]
FBtr0079617; FBpp0079237; FBgn0003502. [P08630-1]
FBtr0112836; FBpp0111749; FBgn0003502. [P08630-1]
GeneIDi34132.
KEGGidme:Dmel_CG8049.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16599 mRNA. Translation: AAA28912.1. Frameshift.
AB009840 mRNA. Translation: BAA24063.1.
AB009841 mRNA. Translation: BAA24064.1.
AE014134 Genomic DNA. Translation: AAF52631.3.
AE014134 Genomic DNA. Translation: AAF52632.2.
AE014134 Genomic DNA. Translation: AAN11161.1.
AE014134 Genomic DNA. Translation: AAN11162.1.
AE014134 Genomic DNA. Translation: ABV53648.1.
AE014134 Genomic DNA. Translation: ABV53649.1.
AY069457 mRNA. Translation: AAL39602.1.
AY128441 mRNA. Translation: AAM75034.1.
BT011183 mRNA. Translation: AAR88544.1.
AF044337 mRNA. Translation: AAB99858.1.
X02305 Genomic DNA. Translation: CAA26170.1.
PIRiA23051.
A27807. TVFFDS.
RefSeqiNP_001097120.1. NM_001103650.2. [P08630-2]
NP_001097121.1. NM_001103651.3. [P08630-1]
NP_476745.1. NM_057397.6. [P08630-1]
NP_476746.1. NM_057398.4. [P08630-2]
NP_723369.1. NM_164804.2. [P08630-1]
NP_723370.1. NM_164805.2. [P08630-2]
UniGeneiDm.7306.

3D structure databases

ProteinModelPortaliP08630.
SMRiP08630.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi60259. 13 interactors.
DIPiDIP-23748N.
IntActiP08630. 1 interactor.
MINTiMINT-896102.
STRINGi7227.FBpp0079237.

PTM databases

iPTMnetiP08630.

Proteomic databases

PaxDbiP08630.
PRIDEiP08630.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0079616; FBpp0079236; FBgn0003502. [P08630-1]
FBtr0079617; FBpp0079237; FBgn0003502. [P08630-1]
FBtr0112836; FBpp0111749; FBgn0003502. [P08630-1]
GeneIDi34132.
KEGGidme:Dmel_CG8049.

Organism-specific databases

CTDi34132.
FlyBaseiFBgn0003502. Btk29A.

Phylogenomic databases

eggNOGiKOG0197. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000119011.
InParanoidiP08630.
KOiK07364.
OMAiRTQRGLF.
OrthoDBiEOG091G0D46.
PhylomeDBiP08630.

Enzyme and pathway databases

ReactomeiR-DME-111465. Apoptotic cleavage of cellular proteins.
R-DME-1660499. Synthesis of PIPs at the plasma membrane.
R-DME-512988. Interleukin-3, 5 and GM-CSF signaling.
SignaLinkiP08630.

Miscellaneous databases

ChiTaRSiBtk29A. fly.
GenomeRNAii34132.
PROiP08630.

Gene expression databases

BgeeiFBgn0003502.
ExpressionAtlasiP08630. baseline.
GenevisibleiP08630. DM.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR001562. Znf_Btk_motif.
[Graphical view]
PfamiPF00779. BTK. 1 hit.
PF00169. PH. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00233. PH. 1 hit.
SM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 2 hits.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
PS51113. ZF_BTK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBTKL_DROME
AccessioniPrimary (citable) accession number: P08630
Secondary accession number(s): A4V0F3
, A4V0F4, O45032, O76132, O76133, P11361, Q6NNV0, Q8T0A0, Q9VLQ2, Q9VLQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 25, 2002
Last modified: November 30, 2016
This is version 190 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.