Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

U1 small nuclear ribonucleoprotein 70 kDa

Gene

SNRNP70

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRNP70 binds to the loop I region of U1-snRNA. The truncated isoforms cannot bind U1-snRNA.

GO - Molecular functioni

  • mRNA binding Source: GO_Central
  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB
  • RNA binding Source: UniProtKB
  • U1 snRNA binding Source: GO_Central
  • U1 snRNP binding Source: Ensembl

GO - Biological processi

  • cellular response to retinoic acid Source: Ensembl
  • cellular response to transforming growth factor beta stimulus Source: Ensembl
  • cellular response to tumor necrosis factor Source: Ensembl
  • mRNA processing Source: ProtInc
  • mRNA splicing, via spliceosome Source: UniProtKB
  • negative regulation of chaperone-mediated autophagy Source: ParkinsonsUK-UCL
  • negative regulation of protein refolding Source: ParkinsonsUK-UCL
  • positive regulation of mRNA splicing, via spliceosome Source: Ensembl
  • regulation of ATPase activity Source: ParkinsonsUK-UCL
  • regulation of RNA splicing Source: UniProtKB
  • RNA splicing Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000104852-MONOMER.
ReactomeiR-HSA-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
U1 small nuclear ribonucleoprotein 70 kDa
Short name:
U1 snRNP 70 kDa
Short name:
U1-70K
Short name:
snRNP70
Gene namesi
Name:SNRNP70
Synonyms:RNPU1Z, RPU1, SNRP70, U1AP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:11150. SNRNP70.

Subcellular locationi

GO - Cellular componenti

  • commitment complex Source: GO_Central
  • cytoplasm Source: Ensembl
  • nuclear speck Source: UniProtKB-SubCell
  • nucleoplasm Source: HPA
  • nucleus Source: HPA
  • precatalytic spliceosome Source: GO_Central
  • spliceosomal complex Source: UniProtKB
  • U1 snRNP Source: UniProtKB
  • U2-type prespliceosome Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi6625.
OpenTargetsiENSG00000104852.
PharmGKBiPA35992.

Polymorphism and mutation databases

BioMutaiSNRNP70.
DMDMi13635663.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000818802 – 437U1 small nuclear ribonucleoprotein 70 kDaAdd BLAST436

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineCombined sources1
Modified residuei118N6-acetyllysineCombined sources1
Modified residuei126PhosphotyrosineCombined sources1
Modified residuei226PhosphoserineCombined sources1
Modified residuei268PhosphoserineCombined sources1
Modified residuei320PhosphoserineCombined sources1
Modified residuei410PhosphoserineCombined sources1

Post-translational modificationi

The N-terminus is blocked.
Extensively phosphorylated on serine residues in the C-terminal region.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP08621.
MaxQBiP08621.
PaxDbiP08621.
PeptideAtlasiP08621.
PRIDEiP08621.

PTM databases

iPTMnetiP08621.
PhosphoSitePlusiP08621.
SwissPalmiP08621.

Miscellaneous databases

PMAP-CutDBP08621.

Expressioni

Gene expression databases

BgeeiENSG00000104852.
ExpressionAtlasiP08621. baseline and differential.
GenevisibleiP08621. HS.

Organism-specific databases

HPAiCAB001718.
HPA042050.
HPA043516.

Interactioni

Subunit structurei

U1 snRNP is composed of the 7 core Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least three U1 snRNP-specific proteins SNRNP70/U1-70K, SNRPA/U1-A and SNRPC/U1-C. Interacts with SCNM1. Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B/SFRS10. Interacts with dephosphorylated SFRS13A and SFPQ. Interacts with NUDT21/CPSF5, CPSF6, SCAF11, and ZRANB2.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CLK2P497602EBI-1049228,EBI-750020
SRPK2P783623EBI-1049228,EBI-593303

Protein-protein interaction databases

BioGridi112509. 205 interactors.
DIPiDIP-29406N.
IntActiP08621. 57 interactors.
MINTiMINT-5004037.
STRINGi9606.ENSP00000472998.

Structurei

Secondary structure

1437
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 10Combined sources4
Helixi29 – 31Combined sources3
Helixi42 – 47Combined sources6
Helixi51 – 53Combined sources3
Helixi63 – 86Combined sources24
Helixi91 – 93Combined sources3
Helixi101 – 103Combined sources3
Beta strandi104 – 109Combined sources6
Helixi116 – 124Combined sources9
Beta strandi129 – 136Combined sources8
Turni138 – 140Combined sources3
Beta strandi143 – 153Combined sources11
Helixi154 – 163Combined sources10
Beta strandi175 – 178Combined sources4
Turni181 – 183Combined sources3
Helixi190 – 192Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2L5INMR-A131-151[»]
2L5JNMR-A131-151[»]
3CW1X-ray5.496/7/8/K1-216[»]
3PGWX-ray4.40L/S1-437[»]
4PJOX-ray3.30K/N/k/n2-60[»]
4PKDX-ray2.50B60-215[»]
ProteinModelPortaliP08621.
SMRiP08621.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08621.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini103 – 181RRMPROSITE-ProRule annotationAdd BLAST79

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi231 – 310Arg/Glu-rich (mixed charge)Add BLAST80
Compositional biasi311 – 314Poly-Gly4
Compositional biasi344 – 393Arg/Asp/Glu-rich (mixed charge)Add BLAST50
Compositional biasi394 – 398Poly-Gly5

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0113. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00530000063750.
InParanoidiP08621.
KOiK11093.
OMAiFDYQNTA.
OrthoDBiEOG091G0R0Z.
PhylomeDBiP08621.
TreeFamiTF314215.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR022023. U1snRNP70_N.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
PF12220. U1snRNP70_N. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P08621-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTQFLPPNLL ALFAPRDPIP YLPPLEKLPH EKHHNQPYCG IAPYIREFED
60 70 80 90 100
PRDAPPPTRA ETREERMERK RREKIERRQQ EVETELKMWD PHNDPNAQGD
110 120 130 140 150
AFKTLFVARV NYDTTESKLR REFEVYGPIK RIHMVYSKRS GKPRGYAFIE
160 170 180 190 200
YEHERDMHSA YKHADGKKID GRRVLVDVER GRTVKGWRPR RLGGGLGGTR
210 220 230 240 250
RGGADVNIRH SGRDDTSRYD ERPGPSPLPH RDRDRDRERE RRERSRERDK
260 270 280 290 300
ERERRRSRSR DRRRRSRSRD KEERRRSRER SKDKDRDRKR RSSRSRERAR
310 320 330 340 350
RERERKEELR GGGGDMAEPS EAGDAPPDDG PPGELGPDGP DGPEEKGRDR
360 370 380 390 400
DRERRRSHRS ERERRRDRDR DRDRDREHKR GERGSERGRD EARGGGGGQD
410 420 430
NGLEGLGNDS RDMYMESEGG DGYLAPENGY LMEAAPE
Length:437
Mass (Da):51,557
Last modified:August 1, 1988 - v2
Checksum:iF1020BF5C40CF97D
GO
Isoform 2 (identifier: P08621-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     223-231: Missing.

Show »
Length:428
Mass (Da):50,618
Checksum:iEEBC1CE4E8B9DBDA
GO
Isoform 3 (identifier: P08621-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     160-166: AYKHADG → TTQLACS
     167-437: Missing.

Show »
Length:166
Mass (Da):19,790
Checksum:iE90F034A08D3E79C
GO
Isoform 4 (identifier: P08621-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-159: MTQFLPPNLL...EYEHERDMHS → MEQALHRFGR...PLPLPTSVTA

Show »
Length:341
Mass (Da):39,191
Checksum:i390967CC272E50E9
GO

Sequence cautioni

The sequence CAA28352 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA29964 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA29966 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0058471 – 159MTQFL…RDMHS → MEQALHRFGRGLVWLSVAWL SVGRVRVRDDGDTGRGFCRA GPVLTRGPSGDSSPLPLPTS VTA in isoform 4. 1 PublicationAdd BLAST159
Alternative sequenceiVSP_005848160 – 166AYKHADG → TTQLACS in isoform 3. 1 Publication7
Alternative sequenceiVSP_005849167 – 437Missing in isoform 3. 1 PublicationAdd BLAST271
Alternative sequenceiVSP_005850223 – 231Missing in isoform 2. Curated9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04654 mRNA. Translation: CAA28352.1. Different initiation.
X06814 mRNA. Translation: CAA29963.1.
X06815 mRNA. Translation: CAA29964.1. Different initiation.
X06817 mRNA. Translation: CAA29966.1. Different initiation.
X07402 mRNA. Translation: CAA30304.1.
X06816 mRNA. Translation: CAA29965.1.
X06812 mRNA. Translation: CAA29961.1.
X06811 mRNA. Translation: CAA29960.1.
X07401 mRNA. Translation: CAA30303.1.
X07403 mRNA. Translation: CAA30305.1.
M22636 mRNA. Translation: AAA03001.1.
M57939
, M57929, M57930, M57932, M57934, M57937 Genomic DNA. Translation: AAA36572.1.
M57939
, M57929, M57930, M57932, M57934, M57937 Genomic DNA. Translation: AAA36573.1.
M57935
, M57929, M57930, M57932, M57934 Genomic DNA. Translation: AAA36571.1.
AK096783 mRNA. Translation: BAG53365.1.
X84841 mRNA. Translation: CAA59278.1.
AL117507 mRNA. Translation: CAB55969.1.
CH471177 Genomic DNA. Translation: EAW52449.1.
BC001315 mRNA. Translation: AAH01315.1.
CCDSiCCDS12756.1. [P08621-1]
CCDS74417.1. [P08621-2]
PIRiA25707.
S00674.
RefSeqiNP_001287998.1. NM_001301069.1. [P08621-2]
NP_003080.2. NM_003089.5. [P08621-1]
UniGeneiHs.467097.

Genome annotation databases

EnsembliENST00000221448; ENSP00000221448; ENSG00000104852. [P08621-2]
ENST00000401730; ENSP00000385077; ENSG00000104852. [P08621-3]
ENST00000595231; ENSP00000471006; ENSG00000104852. [P08621-3]
ENST00000598441; ENSP00000472998; ENSG00000104852. [P08621-1]
ENST00000601065; ENSP00000468952; ENSG00000104852. [P08621-3]
GeneIDi6625.
KEGGihsa:6625.
UCSCiuc002pmk.4. human. [P08621-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04654 mRNA. Translation: CAA28352.1. Different initiation.
X06814 mRNA. Translation: CAA29963.1.
X06815 mRNA. Translation: CAA29964.1. Different initiation.
X06817 mRNA. Translation: CAA29966.1. Different initiation.
X07402 mRNA. Translation: CAA30304.1.
X06816 mRNA. Translation: CAA29965.1.
X06812 mRNA. Translation: CAA29961.1.
X06811 mRNA. Translation: CAA29960.1.
X07401 mRNA. Translation: CAA30303.1.
X07403 mRNA. Translation: CAA30305.1.
M22636 mRNA. Translation: AAA03001.1.
M57939
, M57929, M57930, M57932, M57934, M57937 Genomic DNA. Translation: AAA36572.1.
M57939
, M57929, M57930, M57932, M57934, M57937 Genomic DNA. Translation: AAA36573.1.
M57935
, M57929, M57930, M57932, M57934 Genomic DNA. Translation: AAA36571.1.
AK096783 mRNA. Translation: BAG53365.1.
X84841 mRNA. Translation: CAA59278.1.
AL117507 mRNA. Translation: CAB55969.1.
CH471177 Genomic DNA. Translation: EAW52449.1.
BC001315 mRNA. Translation: AAH01315.1.
CCDSiCCDS12756.1. [P08621-1]
CCDS74417.1. [P08621-2]
PIRiA25707.
S00674.
RefSeqiNP_001287998.1. NM_001301069.1. [P08621-2]
NP_003080.2. NM_003089.5. [P08621-1]
UniGeneiHs.467097.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2L5INMR-A131-151[»]
2L5JNMR-A131-151[»]
3CW1X-ray5.496/7/8/K1-216[»]
3PGWX-ray4.40L/S1-437[»]
4PJOX-ray3.30K/N/k/n2-60[»]
4PKDX-ray2.50B60-215[»]
ProteinModelPortaliP08621.
SMRiP08621.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112509. 205 interactors.
DIPiDIP-29406N.
IntActiP08621. 57 interactors.
MINTiMINT-5004037.
STRINGi9606.ENSP00000472998.

PTM databases

iPTMnetiP08621.
PhosphoSitePlusiP08621.
SwissPalmiP08621.

Polymorphism and mutation databases

BioMutaiSNRNP70.
DMDMi13635663.

Proteomic databases

EPDiP08621.
MaxQBiP08621.
PaxDbiP08621.
PeptideAtlasiP08621.
PRIDEiP08621.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000221448; ENSP00000221448; ENSG00000104852. [P08621-2]
ENST00000401730; ENSP00000385077; ENSG00000104852. [P08621-3]
ENST00000595231; ENSP00000471006; ENSG00000104852. [P08621-3]
ENST00000598441; ENSP00000472998; ENSG00000104852. [P08621-1]
ENST00000601065; ENSP00000468952; ENSG00000104852. [P08621-3]
GeneIDi6625.
KEGGihsa:6625.
UCSCiuc002pmk.4. human. [P08621-1]

Organism-specific databases

CTDi6625.
DisGeNETi6625.
GeneCardsiSNRNP70.
HGNCiHGNC:11150. SNRNP70.
HPAiCAB001718.
HPA042050.
HPA043516.
MIMi180740. gene.
neXtProtiNX_P08621.
OpenTargetsiENSG00000104852.
PharmGKBiPA35992.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0113. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00530000063750.
InParanoidiP08621.
KOiK11093.
OMAiFDYQNTA.
OrthoDBiEOG091G0R0Z.
PhylomeDBiP08621.
TreeFamiTF314215.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000104852-MONOMER.
ReactomeiR-HSA-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

ChiTaRSiSNRNP70. human.
EvolutionaryTraceiP08621.
GeneWikiiSnRNP70.
GenomeRNAii6625.
PMAP-CutDBP08621.
PROiP08621.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000104852.
ExpressionAtlasiP08621. baseline and differential.
GenevisibleiP08621. HS.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR022023. U1snRNP70_N.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
PF12220. U1snRNP70_N. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRU17_HUMAN
AccessioniPrimary (citable) accession number: P08621
Secondary accession number(s): B3KUA3
, P78493, P78494, Q15364, Q15686, Q15687, Q15689, Q99377, Q9UE45, Q9UE46, Q9UE47, Q9UE48, Q9UFQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 2, 2016
This is version 198 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Major ribonucleoprotein antigen recognized by the sera from patients with autoimmune diseases, such as systemic lupus erythematosus.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.