P08587 (TGM2_CAVCU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-glutamine gamma-glutamyltransferase 2
EC=2.3.2.13

Alternative name(s):
Tissue transglutaminase
Transglutaminase C
Short name=TG(C)
Short name=TGC
Short name=TGase C
Transglutaminase-2
Short name=TGase-2

Gene names

Name:

TGM2

Organism

Cavia cutleri (Guinea pig)

Taxonomic identifier

10144 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	690 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
Catalytic activity	Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subunit structure	Monomer.
Sequence similarities	Belongs to the transglutaminase superfamily. Transglutaminase family.

Ontologies

Keywords
Ligand	Calcium Metal-binding
Molecular function	Acyltransferase Transferase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	peptide cross-linking Inferred from electronic annotation. Source: InterPro
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein-glutamine gamma-glutamyltransferase activity Inferred from electronic annotation. Source: EC transaminase activity Inferred from direct assay PubMed 11723121. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2 Ref.3
	Chain	2 – 690	689	Protein-glutamine gamma-glutamyltransferase 2		PRO_0000213706
Sites
	Active site	277	1	By similarity
	Active site	335	1	By similarity
	Active site	358	1	By similarity
	Metal binding	398	1	Calcium By similarity
	Metal binding	400	1	Calcium By similarity
	Metal binding	446	1	Calcium By similarity
	Metal binding	451	1	Calcium By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.3
Experimental info
	Sequence conflict	2 – 3	2	AE → EA AA sequence Ref.2
	Sequence conflict	292	1	G → A in BAA00068. Ref.4
	Sequence conflict	336 – 354	19	CWVES…GYEGW → SLLGGVVDDQAGPGAWVRGV in BAA00068. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P08587 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 047223D38DBEA4A4	FASTA	690	77,141
	10 20 30 40 50 60 MAEDLILERC DLQLEVNGRD HRTADLCRER LVLRRGQPFW LTLHFEGRGY EAGVDTLTFN 70 80 90 100 110 120 AVTGPDPSEE AGTMARFSLS SAVEGGTWSA SAVDQQDSTV SLLLSTPADA PIGLYRLSLE 130 140 150 160 170 180 ASTGYQGSSF VLGHFILLYN PRCPADAVYM DSDQERQEYV LTQQGFIYQG SAKFINGIPW 190 200 210 220 230 240 NFGQFEDGIL DICLMLLDTN PKFLKNAGQD CSRRSRPVYV GRVVSAMVNC NDDQGVLQGR 250 260 270 280 290 300 WDNNYSDGVS PMSWIGSVDI LRRWKDYGCQ RVKYGQCWVF AAVACTVLRC LGIPTRVVTN 310 320 330 340 350 360 FNSAHDQNSN LLIEYFRNES GEIEGNKSEM IWNFHCWVES WMTRPDLEPG YEGWQALDPT 370 380 390 400 410 420 PQEKSEGTYC CGPVPVRAIK EGHLNVKYDA PFVFAEVNAD VVNWIRQKDG SLRKSINHLV 430 440 450 460 470 480 VGLKISTKSV GRDEREDITH TYKYPEGSEE EREAFVRANH LNKLATKEEA QEETGVAMRI 490 500 510 520 530 540 RVGQNMTMGS DFDIFAYITN GTAESHECQL LLCARIVSYN GVLGPVCSTN DLLNLTLDPF 550 560 570 580 590 600 SENSIPLHIL YEKYGDYLTE SNLIKVRGLL IEPAANSYVL AERDIYLENP EIKIRVLGEP 610 620 630 640 650 660 KQNRKLIAEV SLKNPLPVPL LGCIFTVEGA GLTKDQKSVE VPDPVEAGEQ AKVRVDLLPT 670 680 690 EVGLHKLVVN FECDKLKAVK GYRNVIIGPA

References

[1]	"Amino acid sequence of guinea pig liver transglutaminase from its cDNA sequence." Ikura K., Nasu T.-A., Yokota H., Tsuchiya Y., Sasaki R., Chiba H. Biochemistry 27:2898-2905(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Structural properties of guinea pig liver transglutaminase." Connellan J.M., Chung S.I., Whetzel N.K., Bradley L.M., Folk J.E. J. Biol. Chem. 246:1093-1098(1971) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-5. Tissue: Liver.
[3]	"Determination of amino- and carboxyl-terminal sequences of guinea pig liver transglutaminase: evidence for amino-terminal processing." Ikura K., Yokota H., Sasaki R., Chiba H. Biochemistry 28:2344-2348(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-8 AND 684-690, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Liver.
[4]	"Cloning of cDNA coding for guinea pig liver transglutaminase." Ikura K., Nasu T.-A., Yokota H., Sasaki R., Chiba H. Agric. Biol. Chem. 51:957-961(1987) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 189-632. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M19646 mRNA. Translation: AAA37056.1. D00114 mRNA. Translation: BAA00068.1.
PIR	A29996.
3D structure databases
ProteinModelPortal	P08587.
SMR	P08587. Positions 15-690.
ModBase	Search...
Protein-protein interaction databases
IntAct	P08587. 2 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG004342.
Family and domain databases
Gene3D	2.60.40.10. 3 hits.
InterPro	IPR023608. Gln_gamma-glutamylTfrase_euk. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR002931. Transglutaminase-like. IPR008958. Transglutaminase_C. IPR013808. Transglutaminase_CS. IPR001102. Transglutaminase_N. [Graphical view]
PANTHER	PTHR11590. PTHR11590. 1 hit.
Pfam	PF00927. Transglut_C. 2 hits. PF01841. Transglut_core. 1 hit. PF00868. Transglut_N. 1 hit. [Graphical view]
PIRSF	PIRSF000459. TGM_EBP42. 1 hit.
SMART	SM00460. TGc. 1 hit. [Graphical view]
SUPFAM	SSF81296. Ig_E-set. 1 hit. SSF49309. Transglut_C. 2 hits.
PROSITE	PS00547. TRANSGLUTAMINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TGM2_CAVCU

Accession

Primary (citable) accession number: P08587

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 102 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program