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P08582 (TRFM_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 144. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Melanotransferrin
Alternative name(s):
Melanoma-associated antigen p97
CD_antigen=CD228
Gene names
Name:MFI2
Synonyms:MAP97
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length738 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc. Ref.6

Subcellular location

Isoform 1: Cell membrane; Lipid-anchorGPI-anchor.

Tissue specificity

Found predominantly in human melanomas and in certain fetal tissues; also found in liver, epithelium, umbilical chord, placenta and sweat gland ducts.

Sequence similarities

Belongs to the transferrin family.

Contains 2 transferrin-like domains.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P08582-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P08582-2)

The sequence of this isoform differs from the canonical sequence as follows:
     238-302: GKTLPSWGQA...FRLLNEGQRL → ESPSRRQTWT...KRAKSRDMLG
     303-738: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Ref.4
Chain20 – 709690Melanotransferrin Potential
PRO_0000035739
Propeptide710 – 73829Removed in mature form Potential
PRO_0000035740

Regions

Domain23 – 357335Transferrin-like 1
Domain366 – 706341Transferrin-like 2
Region20 – 3011Antigenic epitope

Sites

Metal binding781Iron 1 By similarity
Metal binding1071Iron 1 By similarity
Metal binding2101Iron 1 By similarity
Metal binding2791Iron 1 By similarity
Metal binding4211Iron 2 By similarity
Metal binding4511Iron 2 By similarity
Metal binding5561Iron 2 By similarity
Metal binding6251Iron 2 By similarity
Binding site1321Carbonate 1 By similarity
Binding site1361Carbonate 1 By similarity
Binding site1381Carbonate 1; via amide nitrogen By similarity
Binding site1391Carbonate 1; via amide nitrogen By similarity

Amino acid modifications

Lipidation7091GPI-anchor amidated cysteine Potential
Glycosylation381N-linked (GlcNAc...) Potential
Glycosylation1351N-linked (GlcNAc...) Potential
Glycosylation5151N-linked (GlcNAc...) Potential
Disulfide bond26 ↔ 63 By similarity
Disulfide bond36 ↔ 54 By similarity
Disulfide bond130 ↔ 216 By similarity
Disulfide bond172 ↔ 189 By similarity
Disulfide bond186 ↔ 199 By similarity
Disulfide bond257 ↔ 271 By similarity

Natural variations

Alternative sequence238 – 30265GKTLP…EGQRL → ESPSRRQTWTRSEEEEGECP AHEEARRTMRSSAGQAWKWA PVHRPQDESDKGEFGKRAKS RDMLG in isoform 2.
VSP_006557
Alternative sequence303 – 738436Missing in isoform 2.
VSP_006558
Natural variant2941R → W.
Corresponds to variant rs2276790 [ dbSNP | Ensembl ].
VAR_020413
Natural variant5591A → T.
Corresponds to variant rs17129219 [ dbSNP | Ensembl ].
VAR_057304

Experimental info

Sequence conflict4311T → K in AAA59992. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 28, 2011. Version 2.
Checksum: B0F08B708D2A0A0F

FASTA73880,215
        10         20         30         40         50         60 
MRGPSGALWL LLALRTVLGG MEVRWCATSD PEQHKCGNMS EAFREAGIQP SLLCVRGTSA 

        70         80         90        100        110        120 
DHCVQLIAAQ EADAITLDGG AIYEAGKEHG LKPVVGEVYD QEVGTSYYAV AVVRRSSHVT 

       130        140        150        160        170        180 
IDTLKGVKSC HTGINRTVGW NVPVGYLVES GRLSVMGCDV LKAVSDYFGG SCVPGAGETS 

       190        200        210        220        230        240 
YSESLCRLCR GDSSGEGVCD KSPLERYYDY SGAFRCLAEG AGDVAFVKHS TVLENTDGKT 

       250        260        270        280        290        300 
LPSWGQALLS QDFELLCRDG SRADVTEWRQ CHLARVPAHA VVVRADTDGG LIFRLLNEGQ 

       310        320        330        340        350        360 
RLFSHEGSSF QMFSSEAYGQ KDLLFKDSTS ELVPIATQTY EAWLGHEYLH AMKGLLCDPN 

       370        380        390        400        410        420 
RLPPYLRWCV LSTPEIQKCG DMAVAFRRQR LKPEIQCVSA KSPQHCMERI QAEQVDAVTL 

       430        440        450        460        470        480 
SGEDIYTAGK TYGLVPAAGE HYAPEDSSNS YYVVAVVRRD SSHAFTLDEL RGKRSCHAGF 

       490        500        510        520        530        540 
GSPAGWDVPV GALIQRGFIR PKDCDVLTAV SEFFNASCVP VNNPKNYPSS LCALCVGDEQ 

       550        560        570        580        590        600 
GRNKCVGNSQ ERYYGYRGAF RCLVENAGDV AFVRHTTVFD NTNGHNSEPW AAELRSEDYE 

       610        620        630        640        650        660 
LLCPNGARAE VSQFAACNLA QIPPHAVMVR PDTNIFTVYG LLDKAQDLFG DDHNKNGFKM 

       670        680        690        700        710        720 
FDSSNYHGQD LLFKDATVRA VPVGEKTTYR GWLGLDYVAA LEGMSSQQCS GAAAPAPGAP 

       730 
LLPLLLPALA ARLLPPAL 

« Hide

Isoform 2 [UniParc].

Checksum: 0D51C8347D9EBD8F
Show »

FASTA30232,723

References

« Hide 'large scale' references
[1]"Primary structure of the human melanoma-associated antigen p97 (melanotransferrin) deduced from the mRNA sequence."
Rose T.M., Plowman G.D., Teplow D.B., Dreyer W.J., Hellstroem K.E., Brown J.P.
Proc. Natl. Acad. Sci. U.S.A. 83:1261-1265(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Melanoma.
[2]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Skin and Uterus.
[4]"A unique antigenic epitope of human melanoma is carried on the common melanoma glycoprotein gp95/p97."
Furukawa K.S., Furukawa K., Real F.X., Old L.J., Lloyd K.O.
J. Exp. Med. 169:585-590(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 20-30.
[5]"Transport and expression in human melanomas of a transferrin-like glycosylphosphatidylinositol-anchored protein."
Food M.R., Rothenberger S., Gabathuler R., Haidl I.D., Reid G., Jefferies W.A.
J. Biol. Chem. 269:3034-3040(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: GPI-ANCHOR.
[6]"A novel iron uptake mechanism mediated by GPI-anchored human p97."
Kennard M.L., Richardson D.R., Gabathuler R., Ponka P., Jefferies W.A.
EMBO J. 14:4178-4186(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Human melanotransferrin (p97) has only one functional iron-binding site."
Baker E.N., Baker H.M., Smith C.A., Stebbins M.R., Kahn M., Hellstroem K.E., Hellstroem I.
FEBS Lett. 298:215-218(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: IRON-BINDING.
[8]"A molecular model for the tumour-associated antigen, p97, suggests a Zn-binding function."
Garrat R.C., Jhoti H.
FEBS Lett. 305:55-61(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M12154 mRNA. Translation: AAA59992.1.
AC068302 Genomic DNA. No translation available.
BC001875 mRNA. Translation: AAH01875.1.
BC002623 mRNA. Translation: AAH02623.1.
BC007550 mRNA. Translation: AAH07550.1.
BC071910 mRNA. Translation: AAH71910.1.
PIRTFHUM. A23814.
RefSeqNP_005920.2. NM_005929.5.
NP_201573.1. NM_033316.3.
UniGeneHs.184727.

3D structure databases

ProteinModelPortalP08582.
SMRP08582. Positions 23-710.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110399. 1 interaction.
IntActP08582. 1 interaction.
MINTMINT-5000908.
STRING9606.ENSP00000296350.

Protein family/group databases

MEROPSS60.973.

PTM databases

PhosphoSiteP08582.

Polymorphism databases

DMDM338817914.

Proteomic databases

PaxDbP08582.
PRIDEP08582.

Protocols and materials databases

DNASU4241.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000296350; ENSP00000296350; ENSG00000163975. [P08582-1]
ENST00000296351; ENSP00000296351; ENSG00000163975. [P08582-2]
GeneID4241.
KEGGhsa:4241.
UCSCuc003fxk.4. human. [P08582-1]

Organism-specific databases

CTD4241.
GeneCardsGC03M196715.
H-InvDBHIX0031062.
HGNCHGNC:7037. MFI2.
HPAHPA004880.
MIM155750. gene.
neXtProtNX_P08582.
PharmGKBPA30774.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG87503.
HOGENOMHOG000043759.
HOVERGENHBG000055.
InParanoidP08582.
KOK06569.
OMATAVSEFF.
OrthoDBEOG7D59N7.
PhylomeDBP08582.
TreeFamTF324013.

Gene expression databases

ArrayExpressP08582.
BgeeP08582.
CleanExHS_MFI2.
GenevestigatorP08582.

Family and domain databases

InterProIPR016357. Transferrin.
IPR001156. Transferrin_fam.
IPR018195. Transferrin_Fe_BS.
[Graphical view]
PfamPF00405. Transferrin. 2 hits.
[Graphical view]
PIRSFPIRSF002549. Transferrin. 1 hit.
PRINTSPR00422. TRANSFERRIN.
SMARTSM00094. TR_FER. 2 hits.
[Graphical view]
PROSITEPS00205. TRANSFERRIN_LIKE_1. 2 hits.
PS00206. TRANSFERRIN_LIKE_2. 2 hits.
PS00207. TRANSFERRIN_LIKE_3. 2 hits.
PS51408. TRANSFERRIN_LIKE_4. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiMelanotransferrin.
GenomeRNAi4241.
NextBio16723.
PROP08582.
SOURCESearch...

Entry information

Entry nameTRFM_HUMAN
AccessionPrimary (citable) accession number: P08582
Secondary accession number(s): Q9BQE2
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: June 28, 2011
Last modified: April 16, 2014
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries