Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P08582

- TRFM_HUMAN

UniProt

P08582 - TRFM_HUMAN

Protein

Melanotransferrin

Gene

MFI2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 149 (01 Oct 2014)
      Sequence version 2 (28 Jun 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi78 – 781Iron 1PROSITE-ProRule annotation
    Metal bindingi107 – 1071Iron 1PROSITE-ProRule annotation
    Binding sitei132 – 1321Carbonate 1PROSITE-ProRule annotation
    Binding sitei136 – 1361Carbonate 1PROSITE-ProRule annotation
    Binding sitei138 – 1381Carbonate 1; via amide nitrogenPROSITE-ProRule annotation
    Binding sitei139 – 1391Carbonate 1; via amide nitrogenPROSITE-ProRule annotation
    Metal bindingi210 – 2101Iron 1PROSITE-ProRule annotation
    Metal bindingi279 – 2791Iron 1PROSITE-ProRule annotation
    Metal bindingi421 – 4211Iron 2PROSITE-ProRule annotation
    Metal bindingi451 – 4511Iron 2PROSITE-ProRule annotation
    Metal bindingi556 – 5561Iron 2PROSITE-ProRule annotation
    Metal bindingi625 – 6251Iron 2PROSITE-ProRule annotation

    GO - Molecular functioni

    1. ferric iron binding Source: InterPro
    2. iron ion binding Source: UniProt
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. cellular iron ion homeostasis Source: InterPro
    2. iron ion import Source: UniProt
    3. negative regulation of substrate adhesion-dependent cell spreading Source: UniProt
    4. positive regulation of extracellular matrix disassembly Source: UniProt
    5. positive regulation of plasminogen activation Source: UniProt

    Keywords - Biological processi

    Ion transport, Iron transport, Transport

    Keywords - Ligandi

    Iron, Metal-binding, Zinc

    Protein family/group databases

    MEROPSiS60.973.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Melanotransferrin
    Alternative name(s):
    Melanoma-associated antigen p97
    CD_antigen: CD228
    Gene namesi
    Name:MFI2
    Synonyms:MAP97
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:7037. MFI2.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of plasma membrane Source: UniProt
    2. cell surface Source: UniProt
    3. extracellular vesicular exosome Source: UniProt
    4. integral component of plasma membrane Source: ProtInc

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA30774.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 19191 PublicationAdd
    BLAST
    Chaini20 – 709690MelanotransferrinSequence AnalysisPRO_0000035739Add
    BLAST
    Propeptidei710 – 73829Removed in mature formSequence AnalysisPRO_0000035740Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi26 ↔ 63PROSITE-ProRule annotation
    Disulfide bondi36 ↔ 54PROSITE-ProRule annotation
    Glycosylationi38 – 381N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi130 ↔ 216PROSITE-ProRule annotation
    Glycosylationi135 – 1351N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi172 ↔ 189PROSITE-ProRule annotation
    Disulfide bondi186 ↔ 199PROSITE-ProRule annotation
    Disulfide bondi257 ↔ 271PROSITE-ProRule annotation
    Glycosylationi515 – 5151N-linked (GlcNAc...)Sequence Analysis
    Lipidationi709 – 7091GPI-anchor amidated cysteineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    MaxQBiP08582.
    PaxDbiP08582.
    PRIDEiP08582.

    PTM databases

    PhosphoSiteiP08582.

    Expressioni

    Tissue specificityi

    Found predominantly in human melanomas and in certain fetal tissues; also found in liver, epithelium, umbilical chord, placenta and sweat gland ducts.

    Gene expression databases

    ArrayExpressiP08582.
    BgeeiP08582.
    CleanExiHS_MFI2.
    GenevestigatoriP08582.

    Organism-specific databases

    HPAiHPA004880.

    Interactioni

    Protein-protein interaction databases

    BioGridi110399. 1 interaction.
    IntActiP08582. 1 interaction.
    MINTiMINT-5000908.
    STRINGi9606.ENSP00000296350.

    Structurei

    3D structure databases

    ProteinModelPortaliP08582.
    SMRiP08582. Positions 23-710.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini23 – 357335Transferrin-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini366 – 706341Transferrin-like 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni20 – 3011Antigenic epitopeAdd
    BLAST

    Sequence similaritiesi

    Belongs to the transferrin family.PROSITE-ProRule annotation
    Contains 2 transferrin-like domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG87503.
    HOGENOMiHOG000043759.
    HOVERGENiHBG000055.
    InParanoidiP08582.
    KOiK06569.
    OMAiTAVSEFF.
    OrthoDBiEOG7D59N7.
    PhylomeDBiP08582.
    TreeFamiTF324013.

    Family and domain databases

    InterProiIPR016357. Transferrin.
    IPR001156. Transferrin-like_dom.
    IPR018195. Transferrin_Fe_BS.
    [Graphical view]
    PfamiPF00405. Transferrin. 2 hits.
    [Graphical view]
    PIRSFiPIRSF002549. Transferrin. 1 hit.
    PRINTSiPR00422. TRANSFERRIN.
    SMARTiSM00094. TR_FER. 2 hits.
    [Graphical view]
    PROSITEiPS00205. TRANSFERRIN_LIKE_1. 2 hits.
    PS00206. TRANSFERRIN_LIKE_2. 2 hits.
    PS00207. TRANSFERRIN_LIKE_3. 2 hits.
    PS51408. TRANSFERRIN_LIKE_4. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P08582-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRGPSGALWL LLALRTVLGG MEVRWCATSD PEQHKCGNMS EAFREAGIQP    50
    SLLCVRGTSA DHCVQLIAAQ EADAITLDGG AIYEAGKEHG LKPVVGEVYD 100
    QEVGTSYYAV AVVRRSSHVT IDTLKGVKSC HTGINRTVGW NVPVGYLVES 150
    GRLSVMGCDV LKAVSDYFGG SCVPGAGETS YSESLCRLCR GDSSGEGVCD 200
    KSPLERYYDY SGAFRCLAEG AGDVAFVKHS TVLENTDGKT LPSWGQALLS 250
    QDFELLCRDG SRADVTEWRQ CHLARVPAHA VVVRADTDGG LIFRLLNEGQ 300
    RLFSHEGSSF QMFSSEAYGQ KDLLFKDSTS ELVPIATQTY EAWLGHEYLH 350
    AMKGLLCDPN RLPPYLRWCV LSTPEIQKCG DMAVAFRRQR LKPEIQCVSA 400
    KSPQHCMERI QAEQVDAVTL SGEDIYTAGK TYGLVPAAGE HYAPEDSSNS 450
    YYVVAVVRRD SSHAFTLDEL RGKRSCHAGF GSPAGWDVPV GALIQRGFIR 500
    PKDCDVLTAV SEFFNASCVP VNNPKNYPSS LCALCVGDEQ GRNKCVGNSQ 550
    ERYYGYRGAF RCLVENAGDV AFVRHTTVFD NTNGHNSEPW AAELRSEDYE 600
    LLCPNGARAE VSQFAACNLA QIPPHAVMVR PDTNIFTVYG LLDKAQDLFG 650
    DDHNKNGFKM FDSSNYHGQD LLFKDATVRA VPVGEKTTYR GWLGLDYVAA 700
    LEGMSSQQCS GAAAPAPGAP LLPLLLPALA ARLLPPAL 738
    Length:738
    Mass (Da):80,215
    Last modified:June 28, 2011 - v2
    Checksum:iB0F08B708D2A0A0F
    GO
    Isoform 2 (identifier: P08582-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         238-302: GKTLPSWGQA...FRLLNEGQRL → ESPSRRQTWT...KRAKSRDMLG
         303-738: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:302
    Mass (Da):32,723
    Checksum:i0D51C8347D9EBD8F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti431 – 4311T → K in AAA59992. (PubMed:2419904)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti294 – 2941R → W.
    Corresponds to variant rs2276790 [ dbSNP | Ensembl ].
    VAR_020413
    Natural varianti559 – 5591A → T.
    Corresponds to variant rs17129219 [ dbSNP | Ensembl ].
    VAR_057304

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei238 – 30265GKTLP…EGQRL → ESPSRRQTWTRSEEEEGECP AHEEARRTMRSSAGQAWKWA PVHRPQDESDKGEFGKRAKS RDMLG in isoform 2. 1 PublicationVSP_006557Add
    BLAST
    Alternative sequencei303 – 738436Missing in isoform 2. 1 PublicationVSP_006558Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M12154 mRNA. Translation: AAA59992.1.
    AC068302 Genomic DNA. No translation available.
    BC001875 mRNA. Translation: AAH01875.1.
    BC002623 mRNA. Translation: AAH02623.1.
    BC007550 mRNA. Translation: AAH07550.1.
    BC071910 mRNA. Translation: AAH71910.1.
    CCDSiCCDS3325.1. [P08582-1]
    CCDS3326.1. [P08582-2]
    PIRiA23814. TFHUM.
    RefSeqiNP_005920.2. NM_005929.5. [P08582-1]
    NP_201573.1. NM_033316.3. [P08582-2]
    UniGeneiHs.184727.

    Genome annotation databases

    EnsembliENST00000296350; ENSP00000296350; ENSG00000163975. [P08582-1]
    ENST00000296351; ENSP00000296351; ENSG00000163975. [P08582-2]
    GeneIDi4241.
    KEGGihsa:4241.
    UCSCiuc003fxk.4. human. [P08582-1]

    Polymorphism databases

    DMDMi338817914.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M12154 mRNA. Translation: AAA59992.1 .
    AC068302 Genomic DNA. No translation available.
    BC001875 mRNA. Translation: AAH01875.1 .
    BC002623 mRNA. Translation: AAH02623.1 .
    BC007550 mRNA. Translation: AAH07550.1 .
    BC071910 mRNA. Translation: AAH71910.1 .
    CCDSi CCDS3325.1. [P08582-1 ]
    CCDS3326.1. [P08582-2 ]
    PIRi A23814. TFHUM.
    RefSeqi NP_005920.2. NM_005929.5. [P08582-1 ]
    NP_201573.1. NM_033316.3. [P08582-2 ]
    UniGenei Hs.184727.

    3D structure databases

    ProteinModelPortali P08582.
    SMRi P08582. Positions 23-710.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110399. 1 interaction.
    IntActi P08582. 1 interaction.
    MINTi MINT-5000908.
    STRINGi 9606.ENSP00000296350.

    Protein family/group databases

    MEROPSi S60.973.

    PTM databases

    PhosphoSitei P08582.

    Polymorphism databases

    DMDMi 338817914.

    Proteomic databases

    MaxQBi P08582.
    PaxDbi P08582.
    PRIDEi P08582.

    Protocols and materials databases

    DNASUi 4241.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000296350 ; ENSP00000296350 ; ENSG00000163975 . [P08582-1 ]
    ENST00000296351 ; ENSP00000296351 ; ENSG00000163975 . [P08582-2 ]
    GeneIDi 4241.
    KEGGi hsa:4241.
    UCSCi uc003fxk.4. human. [P08582-1 ]

    Organism-specific databases

    CTDi 4241.
    GeneCardsi GC03M196715.
    H-InvDB HIX0031062.
    HGNCi HGNC:7037. MFI2.
    HPAi HPA004880.
    MIMi 155750. gene.
    neXtProti NX_P08582.
    PharmGKBi PA30774.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG87503.
    HOGENOMi HOG000043759.
    HOVERGENi HBG000055.
    InParanoidi P08582.
    KOi K06569.
    OMAi TAVSEFF.
    OrthoDBi EOG7D59N7.
    PhylomeDBi P08582.
    TreeFami TF324013.

    Miscellaneous databases

    GeneWikii Melanotransferrin.
    GenomeRNAii 4241.
    NextBioi 16723.
    PROi P08582.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P08582.
    Bgeei P08582.
    CleanExi HS_MFI2.
    Genevestigatori P08582.

    Family and domain databases

    InterProi IPR016357. Transferrin.
    IPR001156. Transferrin-like_dom.
    IPR018195. Transferrin_Fe_BS.
    [Graphical view ]
    Pfami PF00405. Transferrin. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF002549. Transferrin. 1 hit.
    PRINTSi PR00422. TRANSFERRIN.
    SMARTi SM00094. TR_FER. 2 hits.
    [Graphical view ]
    PROSITEi PS00205. TRANSFERRIN_LIKE_1. 2 hits.
    PS00206. TRANSFERRIN_LIKE_2. 2 hits.
    PS00207. TRANSFERRIN_LIKE_3. 2 hits.
    PS51408. TRANSFERRIN_LIKE_4. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of the human melanoma-associated antigen p97 (melanotransferrin) deduced from the mRNA sequence."
      Rose T.M., Plowman G.D., Teplow D.B., Dreyer W.J., Hellstroem K.E., Brown J.P.
      Proc. Natl. Acad. Sci. U.S.A. 83:1261-1265(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Melanoma.
    2. "The DNA sequence, annotation and analysis of human chromosome 3."
      Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
      , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
      Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Skin and Uterus.
    4. "A unique antigenic epitope of human melanoma is carried on the common melanoma glycoprotein gp95/p97."
      Furukawa K.S., Furukawa K., Real F.X., Old L.J., Lloyd K.O.
      J. Exp. Med. 169:585-590(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 20-30.
    5. "Transport and expression in human melanomas of a transferrin-like glycosylphosphatidylinositol-anchored protein."
      Food M.R., Rothenberger S., Gabathuler R., Haidl I.D., Reid G., Jefferies W.A.
      J. Biol. Chem. 269:3034-3040(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: GPI-ANCHOR.
    6. "A novel iron uptake mechanism mediated by GPI-anchored human p97."
      Kennard M.L., Richardson D.R., Gabathuler R., Ponka P., Jefferies W.A.
      EMBO J. 14:4178-4186(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Human melanotransferrin (p97) has only one functional iron-binding site."
      Baker E.N., Baker H.M., Smith C.A., Stebbins M.R., Kahn M., Hellstroem K.E., Hellstroem I.
      FEBS Lett. 298:215-218(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: IRON-BINDING.
    8. "A molecular model for the tumour-associated antigen, p97, suggests a Zn-binding function."
      Garrat R.C., Jhoti H.
      FEBS Lett. 305:55-61(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING.

    Entry informationi

    Entry nameiTRFM_HUMAN
    AccessioniPrimary (citable) accession number: P08582
    Secondary accession number(s): Q9BQE2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 149 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human cell differentiation molecules
      CD nomenclature of surface proteins of human leucocytes and list of entries
    2. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3