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Protein

Melanotransferrin

Gene

MFI2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi78 – 781Iron 1PROSITE-ProRule annotation
Metal bindingi107 – 1071Iron 1PROSITE-ProRule annotation
Binding sitei132 – 1321Carbonate 1PROSITE-ProRule annotation
Binding sitei136 – 1361Carbonate 1PROSITE-ProRule annotation
Binding sitei138 – 1381Carbonate 1; via amide nitrogenPROSITE-ProRule annotation
Binding sitei139 – 1391Carbonate 1; via amide nitrogenPROSITE-ProRule annotation
Metal bindingi210 – 2101Iron 1PROSITE-ProRule annotation
Metal bindingi279 – 2791Iron 1PROSITE-ProRule annotation
Metal bindingi421 – 4211Iron 2PROSITE-ProRule annotation
Metal bindingi451 – 4511Iron 2PROSITE-ProRule annotation
Metal bindingi556 – 5561Iron 2PROSITE-ProRule annotation
Metal bindingi625 – 6251Iron 2PROSITE-ProRule annotation

GO - Molecular functioni

  • iron ion binding Source: UniProtKB

GO - Biological processi

  • iron ion homeostasis Source: UniProtKB-KW
  • iron ion import Source: UniProtKB
  • negative regulation of substrate adhesion-dependent cell spreading Source: UniProtKB
  • positive regulation of extracellular matrix disassembly Source: UniProtKB
  • positive regulation of plasminogen activation Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron, Metal-binding, Zinc

Protein family/group databases

MEROPSiS60.973.

Names & Taxonomyi

Protein namesi
Recommended name:
Melanotransferrin
Alternative name(s):
Melanoma-associated antigen p97
CD_antigen: CD228
Gene namesi
Name:MFI2
Synonyms:MAP97
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:7037. MFI2.

Subcellular locationi

GO - Cellular componenti

  • anchored component of plasma membrane Source: UniProtKB
  • cell surface Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • extracellular space Source: InterPro
  • integral component of plasma membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30774.

Polymorphism and mutation databases

BioMutaiMFI2.
DMDMi338817914.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 19191 PublicationAdd
BLAST
Chaini20 – 709690MelanotransferrinSequence AnalysisPRO_0000035739Add
BLAST
Propeptidei710 – 73829Removed in mature formSequence AnalysisPRO_0000035740Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi26 ↔ 63PROSITE-ProRule annotation
Disulfide bondi36 ↔ 54PROSITE-ProRule annotation
Glycosylationi38 – 381N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi130 ↔ 216PROSITE-ProRule annotation
Glycosylationi135 – 1351N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi172 ↔ 189PROSITE-ProRule annotation
Disulfide bondi186 ↔ 199PROSITE-ProRule annotation
Disulfide bondi257 ↔ 271PROSITE-ProRule annotation
Glycosylationi515 – 5151N-linked (GlcNAc...)Sequence Analysis
Lipidationi709 – 7091GPI-anchor amidated cysteineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiP08582.
PaxDbiP08582.
PRIDEiP08582.

PTM databases

PhosphoSiteiP08582.

Expressioni

Tissue specificityi

Found predominantly in human melanomas and in certain fetal tissues; also found in liver, epithelium, umbilical chord, placenta and sweat gland ducts.

Gene expression databases

BgeeiP08582.
CleanExiHS_MFI2.
ExpressionAtlasiP08582. baseline and differential.
GenevisibleiP08582. HS.

Organism-specific databases

HPAiHPA004880.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
HPGDSO607603EBI-10195914,EBI-10187349
NOTCH2NLQ7Z3S93EBI-10195914,EBI-945833

Protein-protein interaction databases

BioGridi110399. 4 interactions.
IntActiP08582. 3 interactions.
MINTiMINT-5000908.
STRINGi9606.ENSP00000296350.

Structurei

3D structure databases

ProteinModelPortaliP08582.
SMRiP08582. Positions 23-710.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 357335Transferrin-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini366 – 706341Transferrin-like 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni20 – 3011Antigenic epitopeAdd
BLAST

Sequence similaritiesi

Belongs to the transferrin family.PROSITE-ProRule annotation
Contains 2 transferrin-like domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG87503.
GeneTreeiENSGT00390000001619.
HOGENOMiHOG000043759.
HOVERGENiHBG000055.
InParanoidiP08582.
KOiK06569.
OMAiFDNTNGH.
OrthoDBiEOG7D59N7.
PhylomeDBiP08582.
TreeFamiTF324013.

Family and domain databases

InterProiIPR029773. MTF.
IPR016357. Transferrin.
IPR001156. Transferrin-like_dom.
IPR018195. Transferrin_Fe_BS.
[Graphical view]
PANTHERiPTHR11485:SF5. PTHR11485:SF5. 1 hit.
PfamiPF00405. Transferrin. 2 hits.
[Graphical view]
PIRSFiPIRSF002549. Transferrin. 1 hit.
PRINTSiPR00422. TRANSFERRIN.
SMARTiSM00094. TR_FER. 2 hits.
[Graphical view]
PROSITEiPS00205. TRANSFERRIN_LIKE_1. 2 hits.
PS00206. TRANSFERRIN_LIKE_2. 2 hits.
PS00207. TRANSFERRIN_LIKE_3. 2 hits.
PS51408. TRANSFERRIN_LIKE_4. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P08582-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGPSGALWL LLALRTVLGG MEVRWCATSD PEQHKCGNMS EAFREAGIQP
60 70 80 90 100
SLLCVRGTSA DHCVQLIAAQ EADAITLDGG AIYEAGKEHG LKPVVGEVYD
110 120 130 140 150
QEVGTSYYAV AVVRRSSHVT IDTLKGVKSC HTGINRTVGW NVPVGYLVES
160 170 180 190 200
GRLSVMGCDV LKAVSDYFGG SCVPGAGETS YSESLCRLCR GDSSGEGVCD
210 220 230 240 250
KSPLERYYDY SGAFRCLAEG AGDVAFVKHS TVLENTDGKT LPSWGQALLS
260 270 280 290 300
QDFELLCRDG SRADVTEWRQ CHLARVPAHA VVVRADTDGG LIFRLLNEGQ
310 320 330 340 350
RLFSHEGSSF QMFSSEAYGQ KDLLFKDSTS ELVPIATQTY EAWLGHEYLH
360 370 380 390 400
AMKGLLCDPN RLPPYLRWCV LSTPEIQKCG DMAVAFRRQR LKPEIQCVSA
410 420 430 440 450
KSPQHCMERI QAEQVDAVTL SGEDIYTAGK TYGLVPAAGE HYAPEDSSNS
460 470 480 490 500
YYVVAVVRRD SSHAFTLDEL RGKRSCHAGF GSPAGWDVPV GALIQRGFIR
510 520 530 540 550
PKDCDVLTAV SEFFNASCVP VNNPKNYPSS LCALCVGDEQ GRNKCVGNSQ
560 570 580 590 600
ERYYGYRGAF RCLVENAGDV AFVRHTTVFD NTNGHNSEPW AAELRSEDYE
610 620 630 640 650
LLCPNGARAE VSQFAACNLA QIPPHAVMVR PDTNIFTVYG LLDKAQDLFG
660 670 680 690 700
DDHNKNGFKM FDSSNYHGQD LLFKDATVRA VPVGEKTTYR GWLGLDYVAA
710 720 730
LEGMSSQQCS GAAAPAPGAP LLPLLLPALA ARLLPPAL
Length:738
Mass (Da):80,215
Last modified:June 28, 2011 - v2
Checksum:iB0F08B708D2A0A0F
GO
Isoform 2 (identifier: P08582-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     238-302: GKTLPSWGQA...FRLLNEGQRL → ESPSRRQTWT...KRAKSRDMLG
     303-738: Missing.

Note: No experimental confirmation available.
Show »
Length:302
Mass (Da):32,723
Checksum:i0D51C8347D9EBD8F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti431 – 4311T → K in AAA59992 (PubMed:2419904).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti294 – 2941R → W.
Corresponds to variant rs2276790 [ dbSNP | Ensembl ].
VAR_020413
Natural varianti559 – 5591A → T.
Corresponds to variant rs17129219 [ dbSNP | Ensembl ].
VAR_057304

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei238 – 30265GKTLP…EGQRL → ESPSRRQTWTRSEEEEGECP AHEEARRTMRSSAGQAWKWA PVHRPQDESDKGEFGKRAKS RDMLG in isoform 2. 1 PublicationVSP_006557Add
BLAST
Alternative sequencei303 – 738436Missing in isoform 2. 1 PublicationVSP_006558Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12154 mRNA. Translation: AAA59992.1.
AC068302 Genomic DNA. No translation available.
BC001875 mRNA. Translation: AAH01875.1.
BC002623 mRNA. Translation: AAH02623.1.
BC007550 mRNA. Translation: AAH07550.1.
BC071910 mRNA. Translation: AAH71910.1.
CCDSiCCDS3325.1. [P08582-1]
CCDS3326.1. [P08582-2]
PIRiA23814. TFHUM.
RefSeqiNP_005920.2. NM_005929.5. [P08582-1]
NP_201573.1. NM_033316.3. [P08582-2]
UniGeneiHs.184727.

Genome annotation databases

EnsembliENST00000296350; ENSP00000296350; ENSG00000163975.
GeneIDi4241.
KEGGihsa:4241.
UCSCiuc003fxk.4. human. [P08582-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12154 mRNA. Translation: AAA59992.1.
AC068302 Genomic DNA. No translation available.
BC001875 mRNA. Translation: AAH01875.1.
BC002623 mRNA. Translation: AAH02623.1.
BC007550 mRNA. Translation: AAH07550.1.
BC071910 mRNA. Translation: AAH71910.1.
CCDSiCCDS3325.1. [P08582-1]
CCDS3326.1. [P08582-2]
PIRiA23814. TFHUM.
RefSeqiNP_005920.2. NM_005929.5. [P08582-1]
NP_201573.1. NM_033316.3. [P08582-2]
UniGeneiHs.184727.

3D structure databases

ProteinModelPortaliP08582.
SMRiP08582. Positions 23-710.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110399. 4 interactions.
IntActiP08582. 3 interactions.
MINTiMINT-5000908.
STRINGi9606.ENSP00000296350.

Protein family/group databases

MEROPSiS60.973.

PTM databases

PhosphoSiteiP08582.

Polymorphism and mutation databases

BioMutaiMFI2.
DMDMi338817914.

Proteomic databases

MaxQBiP08582.
PaxDbiP08582.
PRIDEiP08582.

Protocols and materials databases

DNASUi4241.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000296350; ENSP00000296350; ENSG00000163975.
GeneIDi4241.
KEGGihsa:4241.
UCSCiuc003fxk.4. human. [P08582-1]

Organism-specific databases

CTDi4241.
GeneCardsiGC03M196715.
H-InvDBHIX0031062.
HGNCiHGNC:7037. MFI2.
HPAiHPA004880.
MIMi155750. gene.
neXtProtiNX_P08582.
PharmGKBiPA30774.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG87503.
GeneTreeiENSGT00390000001619.
HOGENOMiHOG000043759.
HOVERGENiHBG000055.
InParanoidiP08582.
KOiK06569.
OMAiFDNTNGH.
OrthoDBiEOG7D59N7.
PhylomeDBiP08582.
TreeFamiTF324013.

Miscellaneous databases

ChiTaRSiMFI2. human.
GeneWikiiMelanotransferrin.
GenomeRNAii4241.
NextBioi16723.
PROiP08582.
SOURCEiSearch...

Gene expression databases

BgeeiP08582.
CleanExiHS_MFI2.
ExpressionAtlasiP08582. baseline and differential.
GenevisibleiP08582. HS.

Family and domain databases

InterProiIPR029773. MTF.
IPR016357. Transferrin.
IPR001156. Transferrin-like_dom.
IPR018195. Transferrin_Fe_BS.
[Graphical view]
PANTHERiPTHR11485:SF5. PTHR11485:SF5. 1 hit.
PfamiPF00405. Transferrin. 2 hits.
[Graphical view]
PIRSFiPIRSF002549. Transferrin. 1 hit.
PRINTSiPR00422. TRANSFERRIN.
SMARTiSM00094. TR_FER. 2 hits.
[Graphical view]
PROSITEiPS00205. TRANSFERRIN_LIKE_1. 2 hits.
PS00206. TRANSFERRIN_LIKE_2. 2 hits.
PS00207. TRANSFERRIN_LIKE_3. 2 hits.
PS51408. TRANSFERRIN_LIKE_4. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure of the human melanoma-associated antigen p97 (melanotransferrin) deduced from the mRNA sequence."
    Rose T.M., Plowman G.D., Teplow D.B., Dreyer W.J., Hellstroem K.E., Brown J.P.
    Proc. Natl. Acad. Sci. U.S.A. 83:1261-1265(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Melanoma.
  2. "The DNA sequence, annotation and analysis of human chromosome 3."
    Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
    , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
    Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Skin and Uterus.
  4. "A unique antigenic epitope of human melanoma is carried on the common melanoma glycoprotein gp95/p97."
    Furukawa K.S., Furukawa K., Real F.X., Old L.J., Lloyd K.O.
    J. Exp. Med. 169:585-590(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 20-30.
  5. "Transport and expression in human melanomas of a transferrin-like glycosylphosphatidylinositol-anchored protein."
    Food M.R., Rothenberger S., Gabathuler R., Haidl I.D., Reid G., Jefferies W.A.
    J. Biol. Chem. 269:3034-3040(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR.
  6. "A novel iron uptake mechanism mediated by GPI-anchored human p97."
    Kennard M.L., Richardson D.R., Gabathuler R., Ponka P., Jefferies W.A.
    EMBO J. 14:4178-4186(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Human melanotransferrin (p97) has only one functional iron-binding site."
    Baker E.N., Baker H.M., Smith C.A., Stebbins M.R., Kahn M., Hellstroem K.E., Hellstroem I.
    FEBS Lett. 298:215-218(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: IRON-BINDING.
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  9. "A molecular model for the tumour-associated antigen, p97, suggests a Zn-binding function."
    Garrat R.C., Jhoti H.
    FEBS Lett. 305:55-61(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING.

Entry informationi

Entry nameiTRFM_HUMAN
AccessioniPrimary (citable) accession number: P08582
Secondary accession number(s): Q9BQE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: June 28, 2011
Last modified: July 22, 2015
This is version 158 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.