Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Melanotransferrin

Gene

MELTF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi78Iron 1PROSITE-ProRule annotation1
Metal bindingi107Iron 1PROSITE-ProRule annotation1
Binding sitei132Carbonate 1PROSITE-ProRule annotation1
Binding sitei136Carbonate 1PROSITE-ProRule annotation1
Binding sitei138Carbonate 1; via amide nitrogenPROSITE-ProRule annotation1
Binding sitei139Carbonate 1; via amide nitrogenPROSITE-ProRule annotation1
Metal bindingi210Iron 1PROSITE-ProRule annotation1
Metal bindingi279Iron 1PROSITE-ProRule annotation1
Metal bindingi421Iron 2PROSITE-ProRule annotation1
Metal bindingi451Iron 2PROSITE-ProRule annotation1
Metal bindingi556Iron 2PROSITE-ProRule annotation1
Metal bindingi625Iron 2PROSITE-ProRule annotation1

GO - Molecular functioni

  • iron ion binding Source: UniProtKB

GO - Biological processi

  • iron ion homeostasis Source: UniProtKB-KW
  • iron ion import Source: UniProtKB
  • negative regulation of substrate adhesion-dependent cell spreading Source: UniProtKB
  • positive regulation of extracellular matrix disassembly Source: UniProtKB
  • positive regulation of plasminogen activation Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000163975-MONOMER.

Protein family/group databases

MEROPSiS60.973.

Names & Taxonomyi

Protein namesi
Recommended name:
MelanotransferrinImported
Alternative name(s):
Melanoma-associated antigen p97
CD_antigen: CD228
Gene namesi
Name:MELTFImported
Synonyms:MAP97, MFI2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:7037. MELTF.

Subcellular locationi

GO - Cellular componenti

  • anchored component of plasma membrane Source: UniProtKB
  • cell surface Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • extracellular space Source: InterPro
  • integral component of plasma membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi4241.
OpenTargetsiENSG00000163975.
PharmGKBiPA30774.

Polymorphism and mutation databases

BioMutaiMFI2.
DMDMi338817914.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 191 PublicationAdd BLAST19
ChainiPRO_000003573920 – 709MelanotransferrinSequence analysisAdd BLAST690
PropeptideiPRO_0000035740710 – 738Removed in mature formSequence analysisAdd BLAST29

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 63PROSITE-ProRule annotation
Disulfide bondi36 ↔ 54PROSITE-ProRule annotation
Glycosylationi38N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi130 ↔ 216PROSITE-ProRule annotation
Glycosylationi135N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi172 ↔ 189PROSITE-ProRule annotation
Disulfide bondi186 ↔ 199PROSITE-ProRule annotation
Disulfide bondi257 ↔ 271PROSITE-ProRule annotation
Modified residuei462Phosphoserine; by FAM20C1 Publication1
Glycosylationi515N-linked (GlcNAc...)Sequence analysis1
Lipidationi709GPI-anchor amidated cysteineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

EPDiP08582.
MaxQBiP08582.
PaxDbiP08582.
PeptideAtlasiP08582.
PRIDEiP08582.

PTM databases

iPTMnetiP08582.
PhosphoSitePlusiP08582.

Expressioni

Tissue specificityi

Found predominantly in human melanomas and in certain fetal tissues; also found in liver, epithelium, umbilical chord, placenta and sweat gland ducts.

Gene expression databases

BgeeiENSG00000163975.
CleanExiHS_MFI2.
ExpressionAtlasiP08582. baseline and differential.
GenevisibleiP08582. HS.

Organism-specific databases

HPAiHPA004880.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
HPGDSO607603EBI-10195914,EBI-10187349
NOTCH2NLQ7Z3S95EBI-10195914,EBI-945833

Protein-protein interaction databases

BioGridi110399. 5 interactors.
IntActiP08582. 6 interactors.
MINTiMINT-5000908.
STRINGi9606.ENSP00000296350.

Structurei

3D structure databases

ProteinModelPortaliP08582.
SMRiP08582.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 357Transferrin-like 1PROSITE-ProRule annotationAdd BLAST335
Domaini366 – 706Transferrin-like 2PROSITE-ProRule annotationAdd BLAST341

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 30Antigenic epitopeAdd BLAST11

Sequence similaritiesi

Belongs to the transferrin family.PROSITE-ProRule annotation
Contains 2 transferrin-like domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IEAI. Eukaryota.
ENOG410XQ36. LUCA.
GeneTreeiENSGT00390000001619.
HOGENOMiHOG000043759.
HOVERGENiHBG000055.
InParanoidiP08582.
KOiK06569.
OMAiFDNTNGH.
OrthoDBiEOG091G0242.
PhylomeDBiP08582.
TreeFamiTF324013.

Family and domain databases

InterProiIPR016357. Transferrin.
IPR001156. Transferrin-like_dom.
IPR018195. Transferrin_Fe_BS.
[Graphical view]
PfamiPF00405. Transferrin. 2 hits.
[Graphical view]
PIRSFiPIRSF002549. Transferrin. 1 hit.
PRINTSiPR00422. TRANSFERRIN.
SMARTiSM00094. TR_FER. 2 hits.
[Graphical view]
PROSITEiPS00205. TRANSFERRIN_LIKE_1. 2 hits.
PS00206. TRANSFERRIN_LIKE_2. 2 hits.
PS00207. TRANSFERRIN_LIKE_3. 2 hits.
PS51408. TRANSFERRIN_LIKE_4. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P08582-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGPSGALWL LLALRTVLGG MEVRWCATSD PEQHKCGNMS EAFREAGIQP
60 70 80 90 100
SLLCVRGTSA DHCVQLIAAQ EADAITLDGG AIYEAGKEHG LKPVVGEVYD
110 120 130 140 150
QEVGTSYYAV AVVRRSSHVT IDTLKGVKSC HTGINRTVGW NVPVGYLVES
160 170 180 190 200
GRLSVMGCDV LKAVSDYFGG SCVPGAGETS YSESLCRLCR GDSSGEGVCD
210 220 230 240 250
KSPLERYYDY SGAFRCLAEG AGDVAFVKHS TVLENTDGKT LPSWGQALLS
260 270 280 290 300
QDFELLCRDG SRADVTEWRQ CHLARVPAHA VVVRADTDGG LIFRLLNEGQ
310 320 330 340 350
RLFSHEGSSF QMFSSEAYGQ KDLLFKDSTS ELVPIATQTY EAWLGHEYLH
360 370 380 390 400
AMKGLLCDPN RLPPYLRWCV LSTPEIQKCG DMAVAFRRQR LKPEIQCVSA
410 420 430 440 450
KSPQHCMERI QAEQVDAVTL SGEDIYTAGK TYGLVPAAGE HYAPEDSSNS
460 470 480 490 500
YYVVAVVRRD SSHAFTLDEL RGKRSCHAGF GSPAGWDVPV GALIQRGFIR
510 520 530 540 550
PKDCDVLTAV SEFFNASCVP VNNPKNYPSS LCALCVGDEQ GRNKCVGNSQ
560 570 580 590 600
ERYYGYRGAF RCLVENAGDV AFVRHTTVFD NTNGHNSEPW AAELRSEDYE
610 620 630 640 650
LLCPNGARAE VSQFAACNLA QIPPHAVMVR PDTNIFTVYG LLDKAQDLFG
660 670 680 690 700
DDHNKNGFKM FDSSNYHGQD LLFKDATVRA VPVGEKTTYR GWLGLDYVAA
710 720 730
LEGMSSQQCS GAAAPAPGAP LLPLLLPALA ARLLPPAL
Length:738
Mass (Da):80,215
Last modified:June 28, 2011 - v2
Checksum:iB0F08B708D2A0A0F
GO
Isoform 2 (identifier: P08582-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     238-302: GKTLPSWGQA...FRLLNEGQRL → ESPSRRQTWT...KRAKSRDMLG
     303-738: Missing.

Note: No experimental confirmation available.
Show »
Length:302
Mass (Da):32,723
Checksum:i0D51C8347D9EBD8F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti431T → K in AAA59992 (PubMed:2419904).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_020413294R → W.Corresponds to variant rs2276790dbSNPEnsembl.1
Natural variantiVAR_057304559A → T.Corresponds to variant rs17129219dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006557238 – 302GKTLP…EGQRL → ESPSRRQTWTRSEEEEGECP AHEEARRTMRSSAGQAWKWA PVHRPQDESDKGEFGKRAKS RDMLG in isoform 2. 1 PublicationAdd BLAST65
Alternative sequenceiVSP_006558303 – 738Missing in isoform 2. 1 PublicationAdd BLAST436

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12154 mRNA. Translation: AAA59992.1.
AC068302 Genomic DNA. No translation available.
BC001875 mRNA. Translation: AAH01875.1.
BC002623 mRNA. Translation: AAH02623.1.
BC007550 mRNA. Translation: AAH07550.1.
BC071910 mRNA. Translation: AAH71910.1.
CCDSiCCDS3325.1. [P08582-1]
CCDS3326.1. [P08582-2]
PIRiA23814. TFHUM.
RefSeqiNP_005920.2. NM_005929.5. [P08582-1]
NP_201573.1. NM_033316.3. [P08582-2]
UniGeneiHs.184727.

Genome annotation databases

EnsembliENST00000296350; ENSP00000296350; ENSG00000163975. [P08582-1]
ENST00000296351; ENSP00000296351; ENSG00000163975. [P08582-2]
GeneIDi4241.
KEGGihsa:4241.
UCSCiuc003fxk.5. human. [P08582-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12154 mRNA. Translation: AAA59992.1.
AC068302 Genomic DNA. No translation available.
BC001875 mRNA. Translation: AAH01875.1.
BC002623 mRNA. Translation: AAH02623.1.
BC007550 mRNA. Translation: AAH07550.1.
BC071910 mRNA. Translation: AAH71910.1.
CCDSiCCDS3325.1. [P08582-1]
CCDS3326.1. [P08582-2]
PIRiA23814. TFHUM.
RefSeqiNP_005920.2. NM_005929.5. [P08582-1]
NP_201573.1. NM_033316.3. [P08582-2]
UniGeneiHs.184727.

3D structure databases

ProteinModelPortaliP08582.
SMRiP08582.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110399. 5 interactors.
IntActiP08582. 6 interactors.
MINTiMINT-5000908.
STRINGi9606.ENSP00000296350.

Protein family/group databases

MEROPSiS60.973.

PTM databases

iPTMnetiP08582.
PhosphoSitePlusiP08582.

Polymorphism and mutation databases

BioMutaiMFI2.
DMDMi338817914.

Proteomic databases

EPDiP08582.
MaxQBiP08582.
PaxDbiP08582.
PeptideAtlasiP08582.
PRIDEiP08582.

Protocols and materials databases

DNASUi4241.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000296350; ENSP00000296350; ENSG00000163975. [P08582-1]
ENST00000296351; ENSP00000296351; ENSG00000163975. [P08582-2]
GeneIDi4241.
KEGGihsa:4241.
UCSCiuc003fxk.5. human. [P08582-1]

Organism-specific databases

CTDi4241.
DisGeNETi4241.
GeneCardsiMFI2.
H-InvDBHIX0031062.
HGNCiHGNC:7037. MELTF.
HPAiHPA004880.
MIMi155750. gene.
neXtProtiNX_P08582.
OpenTargetsiENSG00000163975.
PharmGKBiPA30774.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IEAI. Eukaryota.
ENOG410XQ36. LUCA.
GeneTreeiENSGT00390000001619.
HOGENOMiHOG000043759.
HOVERGENiHBG000055.
InParanoidiP08582.
KOiK06569.
OMAiFDNTNGH.
OrthoDBiEOG091G0242.
PhylomeDBiP08582.
TreeFamiTF324013.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000163975-MONOMER.

Miscellaneous databases

ChiTaRSiMFI2. human.
GeneWikiiMelanotransferrin.
GenomeRNAii4241.
PROiP08582.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000163975.
CleanExiHS_MFI2.
ExpressionAtlasiP08582. baseline and differential.
GenevisibleiP08582. HS.

Family and domain databases

InterProiIPR016357. Transferrin.
IPR001156. Transferrin-like_dom.
IPR018195. Transferrin_Fe_BS.
[Graphical view]
PfamiPF00405. Transferrin. 2 hits.
[Graphical view]
PIRSFiPIRSF002549. Transferrin. 1 hit.
PRINTSiPR00422. TRANSFERRIN.
SMARTiSM00094. TR_FER. 2 hits.
[Graphical view]
PROSITEiPS00205. TRANSFERRIN_LIKE_1. 2 hits.
PS00206. TRANSFERRIN_LIKE_2. 2 hits.
PS00207. TRANSFERRIN_LIKE_3. 2 hits.
PS51408. TRANSFERRIN_LIKE_4. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRFM_HUMAN
AccessioniPrimary (citable) accession number: P08582
Secondary accession number(s): Q9BQE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: June 28, 2011
Last modified: November 30, 2016
This is version 168 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.