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P08579

- RU2B_HUMAN

UniProt

P08579 - RU2B_HUMAN

Protein

U2 small nuclear ribonucleoprotein B''

Gene

SNRPB2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 156 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    Involved in pre-mRNA splicing. This protein is associated with snRNP U2. It binds stem loop IV of U2 snRNA only in presence of the U2A' protein.

    GO - Molecular functioni

    1. nucleotide binding Source: InterPro
    2. protein binding Source: IntAct
    3. snRNA binding Source: InterPro

    GO - Biological processi

    1. gene expression Source: Reactome
    2. mRNA processing Source: ProtInc
    3. mRNA splicing, via spliceosome Source: UniProtKB
    4. RNA splicing Source: Reactome

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_467. mRNA Splicing - Major Pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    U2 small nuclear ribonucleoprotein B''
    Short name:
    U2 snRNP B''
    Gene namesi
    Name:SNRPB2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 20

    Organism-specific databases

    HGNCiHGNC:11155. SNRPB2.

    Subcellular locationi

    GO - Cellular componenti

    1. catalytic step 2 spliceosome Source: UniProtKB
    2. nucleolus Source: HPA
    3. nucleoplasm Source: Reactome
    4. nucleus Source: HPA
    5. spliceosomal complex Source: HGNC
    6. U2 snRNP Source: ProtInc

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35996.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 225225U2 small nuclear ribonucleoprotein B''PRO_0000081892Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei111 – 1111N6-acetyllysineBy similarity
    Modified residuei151 – 1511Phosphotyrosine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP08579.
    PaxDbiP08579.
    PeptideAtlasiP08579.
    PRIDEiP08579.

    PTM databases

    PhosphoSiteiP08579.

    Expressioni

    Gene expression databases

    BgeeiP08579.
    CleanExiHS_SNRPB2.
    GenevestigatoriP08579.

    Organism-specific databases

    HPAiHPA050814.

    Interactioni

    Subunit structurei

    Identified in the spliceosome C complex. Present in a spliceosome complex assembled in vitro, and composed of SNRPB2, HPRP8BP and CRNKL1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SNRPA1P096612EBI-1053651,EBI-876439

    Protein-protein interaction databases

    BioGridi112513. 43 interactions.
    IntActiP08579. 12 interactions.
    MINTiMINT-156501.
    STRINGi9606.ENSP00000246071.

    Structurei

    Secondary structure

    1
    225
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 137
    Helixi20 – 3213
    Beta strandi37 – 415
    Turni46 – 505
    Beta strandi52 – 587
    Helixi59 – 6810
    Beta strandi80 – 834
    Helixi89 – 957

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1A9NX-ray2.38B/D1-96[»]
    ProteinModelPortaliP08579.
    SMRiP08579. Positions 3-96, 146-225.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP08579.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini7 – 8680RRM 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini151 – 22575RRM 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the RRM U1 A/B'' family.Curated
    Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG317159.
    HOGENOMiHOG000217519.
    HOVERGENiHBG000895.
    InParanoidiP08579.
    KOiK11094.
    OMAiPQNPMVI.
    OrthoDBiEOG77DJ77.
    PhylomeDBiP08579.
    TreeFamiTF313834.

    Family and domain databases

    Gene3Di3.30.70.330. 2 hits.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    IPR024888. U1_snRNP_A/U2_snRNP_B''.
    [Graphical view]
    PANTHERiPTHR10501. PTHR10501. 1 hit.
    PfamiPF00076. RRM_1. 1 hit.
    [Graphical view]
    SMARTiSM00360. RRM. 2 hits.
    [Graphical view]
    PROSITEiPS50102. RRM. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P08579-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDIRPNHTIY INNMNDKIKK EELKRSLYAL FSQFGHVVDI VALKTMKMRG    50
    QAFVIFKELG SSTNALRQLQ GFPFYGKPMR IQYAKTDSDI ISKMRGTFAD 100
    KEKKKEKKKA KTVEQTATTT NKKPGQGTPN SANTQGNSTP NPQVPDYPPN 150
    YILFLNNLPE ETNEMMLSML FNQFPGFKEV RLVPGRHDIA FVEFENDGQA 200
    GAARDALQGF KITPSHAMKI TYAKK 225
    Length:225
    Mass (Da):25,486
    Last modified:August 1, 1988 - v1
    Checksum:i67C949CC7E14A92A
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti19 – 191K → Q in a colorectal cancer sample; somatic mutation. 1 Publication
    VAR_035487

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15841 mRNA. Translation: AAA36796.1.
    AK313004 mRNA. Translation: BAG35840.1.
    AL034428 Genomic DNA. Translation: CAB38777.2.
    CH471133 Genomic DNA. Translation: EAX10284.1.
    CH471133 Genomic DNA. Translation: EAX10285.1.
    BC018022 mRNA. Translation: AAH18022.1.
    BC036737 mRNA. Translation: AAH36737.1.
    CCDSiCCDS13123.1.
    PIRiA25910.
    RefSeqiNP_003083.1. NM_003092.4.
    NP_937863.1. NM_198220.2.
    UniGeneiHs.280378.

    Genome annotation databases

    EnsembliENST00000246071; ENSP00000246071; ENSG00000125870.
    ENST00000377943; ENSP00000367178; ENSG00000125870.
    GeneIDi6629.
    KEGGihsa:6629.
    UCSCiuc002wph.2. human.

    Polymorphism databases

    DMDMi134095.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15841 mRNA. Translation: AAA36796.1 .
    AK313004 mRNA. Translation: BAG35840.1 .
    AL034428 Genomic DNA. Translation: CAB38777.2 .
    CH471133 Genomic DNA. Translation: EAX10284.1 .
    CH471133 Genomic DNA. Translation: EAX10285.1 .
    BC018022 mRNA. Translation: AAH18022.1 .
    BC036737 mRNA. Translation: AAH36737.1 .
    CCDSi CCDS13123.1.
    PIRi A25910.
    RefSeqi NP_003083.1. NM_003092.4.
    NP_937863.1. NM_198220.2.
    UniGenei Hs.280378.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1A9N X-ray 2.38 B/D 1-96 [» ]
    ProteinModelPortali P08579.
    SMRi P08579. Positions 3-96, 146-225.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112513. 43 interactions.
    IntActi P08579. 12 interactions.
    MINTi MINT-156501.
    STRINGi 9606.ENSP00000246071.

    PTM databases

    PhosphoSitei P08579.

    Polymorphism databases

    DMDMi 134095.

    Proteomic databases

    MaxQBi P08579.
    PaxDbi P08579.
    PeptideAtlasi P08579.
    PRIDEi P08579.

    Protocols and materials databases

    DNASUi 6629.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000246071 ; ENSP00000246071 ; ENSG00000125870 .
    ENST00000377943 ; ENSP00000367178 ; ENSG00000125870 .
    GeneIDi 6629.
    KEGGi hsa:6629.
    UCSCi uc002wph.2. human.

    Organism-specific databases

    CTDi 6629.
    GeneCardsi GC20P016658.
    HGNCi HGNC:11155. SNRPB2.
    HPAi HPA050814.
    MIMi 603520. gene.
    neXtProti NX_P08579.
    PharmGKBi PA35996.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG317159.
    HOGENOMi HOG000217519.
    HOVERGENi HBG000895.
    InParanoidi P08579.
    KOi K11094.
    OMAi PQNPMVI.
    OrthoDBi EOG77DJ77.
    PhylomeDBi P08579.
    TreeFami TF313834.

    Enzyme and pathway databases

    Reactomei REACT_467. mRNA Splicing - Major Pathway.

    Miscellaneous databases

    EvolutionaryTracei P08579.
    GeneWikii SNRPB2.
    GenomeRNAii 6629.
    NextBioi 25823.
    PROi P08579.
    SOURCEi Search...

    Gene expression databases

    Bgeei P08579.
    CleanExi HS_SNRPB2.
    Genevestigatori P08579.

    Family and domain databases

    Gene3Di 3.30.70.330. 2 hits.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    IPR024888. U1_snRNP_A/U2_snRNP_B''.
    [Graphical view ]
    PANTHERi PTHR10501. PTHR10501. 1 hit.
    Pfami PF00076. RRM_1. 1 hit.
    [Graphical view ]
    SMARTi SM00360. RRM. 2 hits.
    [Graphical view ]
    PROSITEi PS50102. RRM. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of a cDNA clone expressing a human autoimmune antigen: full-length sequence of the U2 small nuclear RNA-associated B' antigen."
      Habets W.J., Sillekens P.T.G., Hoet M.H., Schalken J.A., Roebroek A.J.M., Leunissen J.A.M., de Ven W.J.M., van Venrooij W.J.
      Proc. Natl. Acad. Sci. U.S.A. 84:2421-2425(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Cerebellum.
    3. "The DNA sequence and comparative analysis of human chromosome 20."
      Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
      , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
      Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain and Uterus.
    6. "Crooked neck is a component of the human spliceosome and implicated in the splicing process."
      Chung S., Zhou Z., Huddleston K.A., Harrison D.A., Reed R., Coleman T.A., Rymond B.C.
      Biochim. Biophys. Acta 1576:287-297(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN SPLICEOSOMAL COMPLEX WITH HPRP8BP AND CRNKL1.
    7. "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."
      Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.
      RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
    8. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
      Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
      Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-151, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Crystal structure of the spliceosomal U2B'-U2A' protein complex bound to a fragment of U2 small nuclear RNA."
      Price S.R., Evans P.R., Nagai K.
      Nature 394:645-650(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.38 ANGSTROMS) OF 1-96.
    11. Cited for: VARIANT [LARGE SCALE ANALYSIS] GLN-19.

    Entry informationi

    Entry nameiRU2B_HUMAN
    AccessioniPrimary (citable) accession number: P08579
    Secondary accession number(s): B2R7J3, D3DW21, Q9UJD4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 156 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    Patients with systemic lupus erythematosus produce antibodies which interact with snRNP proteins.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 20
      Human chromosome 20: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3