P08571 (CD14_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Monocyte differentiation antigen CD14

Alternative name(s):
Myeloid cell-specific leucine-rich glycoprotein
CD_antigen=CD14

Cleaved into the following 2 chains:

1. Monocyte differentiation antigen CD14, urinary form
2. Monocyte differentiation antigen CD14, membrane-bound form

Gene names

Name:

CD14

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
Subunit structure	Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Tissue specificity	Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
Post-translational modification	N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. Ref.12 Ref.13 Ref.14
Sequence similarities	Contains 11 LRR (leucine-rich) repeats.

Ontologies

Keywords
Biological process	Immunity Inflammatory response Innate immunity
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Leucine-rich repeat Repeat Signal
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	apoptosis Traceable author statement. Source: ProtInc cell surface receptor linked signaling pathway Traceable author statement. Source: ProtInc cellular response to lipopolysaccharide Inferred from direct assay. Source: MGI cellular response to lipoteichoic acid Inferred from direct assay. Source: MGI inflammatory response Inferred from electronic annotation. Source: UniProtKB-KW innate immune response Inferred from electronic annotation. Source: UniProtKB-KW phagocytosis Traceable author statement. Source: ProtInc positive regulation of tumor necrosis factor production Inferred from direct assay. Source: MGI
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from Experiment. Source: Reactome
Molecular function	lipopolysaccharide binding Inferred from direct assay. Source: MGI lipoteichoic acid binding Inferred from direct assay. Source: MGI opsonin receptor activity Traceable author statement. Source: BHF-UCL peptidoglycan receptor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19
	Chain	20 – 367	348	Monocyte differentiation antigen CD14, urinary form		PRO_0000020884
	Chain	20 – 345	326	Monocyte differentiation antigen CD14, membrane-bound form		PRO_0000020885
	Propeptide	346 – 375	30	Removed in mature form Potential		PRO_0000020886
Regions
	Repeat	54 – 82	29	LRR 1
	Repeat	83 – 118	36	LRR 2
	Repeat	119 – 144	26	LRR 3
	Repeat	145 – 172	28	LRR 4
	Repeat	173 – 196	24	LRR 5
	Repeat	197 – 224	28	LRR 6
	Repeat	225 – 251	27	LRR 7
	Repeat	252 – 278	27	LRR 8
	Repeat	279 – 299	21	LRR 9
	Repeat	300 – 321	22	LRR 10
	Repeat	322 – 349	28	LRR 11
Amino acid modifications
	Lipidation	345	1	GPI-anchor amidated asparagine Potential
	Glycosylation	37	1	N-linked (GlcNAc...) Potential
	Glycosylation	151	1	N-linked (GlcNAc...) Ref.12
	Glycosylation	282	1	N-linked (GlcNAc...) Ref.12
	Glycosylation	323	1	N-linked (GlcNAc...) Ref.13
	Glycosylation	336	1	O-linked (GalNAc...) Ref.14
	Disulfide bond	25 ↔ 36		By similarity
	Disulfide bond	34 ↔ 51		By similarity
	Disulfide bond	187 ↔ 217		By similarity
	Disulfide bond	241 ↔ 272		By similarity
Natural variations
	Natural variant	204	1	N → D. [dbSNP:rs2228049]		VAR_024302
	Natural variant	341	1	E → K. [dbSNP:rs11556179]		VAR_050771
Experimental info
	Sequence conflict	187	1	C → Y in CAA29999. Ref.2
	Sequence conflict	303	1	D → E in AAC83816. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P08571-1 [UniParc]. Last modified March 27, 2002. Version 2. Checksum: 1746CDB41F394F8D	FASTA	375	40,076
	10 20 30 40 50 60 MERASCLLLL LLPLVHVSAT TPEPCELDDE DFRCVCNFSE PQPDWSEAFQ CVSAVEVEIH 70 80 90 100 110 120 AGGLNLEPFL KRVDADADPR QYADTVKALR VRRLTVGAAQ VPAQLLVGAL RVLAYSRLKE 130 140 150 160 170 180 LTLEDLKITG TMPPLPLEAT GLALSSLRLR NVSWATGRSW LAELQQWLKP GLKVLSIAQA 190 200 210 220 230 240 HSPAFSCEQV RAFPALTSLD LSDNPGLGER GLMAALCPHK FPAIQNLALR NTGMETPTGV 250 260 270 280 290 300 CAALAAAGVQ PHSLDLSHNS LRATVNPSAP RCMWSSALNS LNLSFAGLEQ VPKGLPAKLR 310 320 330 340 350 360 VLDLSCNRLN RAPQPDELPE VDNLTLDGNP FLVPGTALPH EGSMNSGVVP ACARSTLSVG 370 VSGTLVLLQG ARGFA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The monocyte differentiation antigen, CD14, is anchored to the cell membrane by a phosphatidylinositol linkage." Haziot A., Chen S., Ferrero E., Low M.G., Silber R., Goyert S.M. J. Immunol. 141:547-552(1988) [PubMed: 3385210] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Nucleotide sequence of the gene encoding the monocyte differentiation antigen, CD14." Ferrero E., Goyert S.M. Nucleic Acids Res. 16:4173-4173(1988) [PubMed: 2453848] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Lymphocyte.
[3]	"Mouse and human CD14 (myeloid cell-specific leucine-rich glycoprotein) primary structure deduced from cDNA clones." Setoguchi M., Nasu N., Yoshida S., Higuchi Y., Akizuki S., Yamamoto S. Biochim. Biophys. Acta 1008:213-222(1989) [PubMed: 2472171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Macrophage.
[4]	"Monocyte antigen CD14 is a phospholipid anchored membrane protein." Simmons D.L., Tan S., Tenen D.G., Nicholson-Weller A., Seed B. Blood 73:284-289(1989) [PubMed: 2462937] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"Cloning and sequencing of human CD14 gene." Long J.Y., Xue Y.N., Sun L., Wang H.X. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Jin Zhan 25:377-378(1998) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Promyelocytic leukemia.
[6]	"Natural selection in the TLR-related genes in the course of primate evolution." Nakajima T., Ohtani H., Satta Y., Uno Y., Akari H., Ishida T., Kimura A. Immunogenetics 60:727-735(2008) [PubMed: 18810425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[7]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[10]	"Expression and secretion of CD14 in glial neoplasms of the brain." Deininger M.H., Meyermann R., Schluesener H.J. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-125. Tissue: Glioblastoma.
[11]	"Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14." Bazil V., Baudys M., Hilgert I., Stefanova I., Low M.G., Zbrozek J., Horejsi V. Mol. Immunol. 26:657-662(1989) [PubMed: 2779588] [Abstract] Cited for: PROTEIN SEQUENCE OF 362-367.
[12]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed: 16335952] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-151 AND ASN-282, MASS SPECTROMETRY. Tissue: Plasma.
[13]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-323, MASS SPECTROMETRY. Tissue: Liver.
[14]	"Enrichment of glycopeptides for glycan structure and attachment site identification." Nilsson J., Rueetschi U., Halim A., Hesse C., Carlsohn E., Brinkmalm G., Larson G. Nat. Methods 6:809-811(2009) [PubMed: 19838169] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT THR-336, STRUCTURE OF CARBOHYDRATES, MASS SPECTROMETRY. Tissue: Cerebrospinal fluid.
+	Additional computationally mapped references.

Web resources

Wikipedia

CD14 entry

Cross-references

Sequence databases
EMBL GenBank DDBJ	X06882 Genomic DNA. Translation: CAA29999.1. X13334 mRNA. Translation: CAA31711.1. M86511 mRNA. Translation: AAA51930.1. AF097942 mRNA. Translation: AAC83816.1. AB446505 mRNA. Translation: BAG55282.1. BT007331 mRNA. Translation: AAP35995.1. CH471062 Genomic DNA. Translation: EAW62037.1. BC010507 mRNA. Translation: AAH10507.1. AY044269 mRNA. Translation: AAL02401.1.
IPI	IPI00029260.
PIR	TDHUM4. A27637.
RefSeq	NP_000582.1. NP_001035110.1. NP_001167575.1. NP_001167576.1.
UniGene	Hs.163867. Hs.638333.
3D structure databases
ProteinModelPortal	P08571.
SMR	P08571. Positions 22-332.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1030N.
STRING	P08571.
Proteomic databases
PeptideAtlas	P08571.
PRIDE	P08571.
Genome annotation databases
Ensembl	ENST00000302014; ENSP00000304236; ENSG00000170458; Homo sapiens. [Genome view] ENST00000401743; ENSP00000385519; ENSG00000170458; Homo sapiens. [Genome view]
GeneID	929.
KEGG	hsa:929.
UCSC	uc003lgi.1. human.
Organism-specific databases
CTD	929.
GeneCards	GC05M139991.
H-InvDB	HIX0005234.
HGNC	HGNC:1628. CD14.
HPA	HPA001887. HPA002127.
MIM	158120. gene.
PharmGKB	PA26188.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG18529.
HOGENOM	HBG125703.
HOVERGEN	HBG005269.
InParanoid	P08571.
OMA	ALCPHKF.
OrthoDB	EOG94N1C5.
PhylomeDB	P08571.
Enzyme and pathway databases
Reactome	REACT_6900. Signaling in Immune system.
Gene expression databases
ArrayExpress	P08571.
Bgee	P08571.
CleanEx	HS_CD14.
Genevestigator	P08571.
GermOnline	ENSG00000170458. Homo sapiens.
Family and domain databases
InterPro	IPR001611. Leu-rich_rpt. IPR016337. Monocyte_diff_Ag_CD14. [Graphical view]
Pfam	PF00560. LRR_1. 1 hit. [Graphical view]
PIRSF	PIRSF002017. CD14. 1 hit.
PROSITE	PS51450. LRR. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	3850.
PMAP-CutDB	P08571.
SOURCE	Search...

Entry information

Entry name

CD14_HUMAN

Accession

Primary (citable) accession number: P08571
Secondary accession number(s): Q53XT5 , Q96FR6, Q96L99, Q9UNS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	March 27, 2002
Last modified:	August 10, 2010
This is version 120 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.