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P08571

- CD14_HUMAN

UniProt

P08571 - CD14_HUMAN

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Protein

Monocyte differentiation antigen CD14

Gene

CD14

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.1 Publication

GO - Molecular functioni

  1. lipopolysaccharide binding Source: MGI
  2. lipoteichoic acid binding Source: MGI
  3. opsonin receptor activity Source: BHF-UCL
  4. peptidoglycan receptor activity Source: ProtInc

GO - Biological processi

  1. apoptotic process Source: ProtInc
  2. cell surface receptor signaling pathway Source: ProtInc
  3. cellular response to lipopolysaccharide Source: MGI
  4. cellular response to lipoteichoic acid Source: MGI
  5. I-kappaB kinase/NF-kappaB signaling Source: Reactome
  6. inflammatory response Source: UniProtKB-KW
  7. innate immune response Source: Reactome
  8. MyD88-dependent toll-like receptor signaling pathway Source: Reactome
  9. MyD88-independent toll-like receptor signaling pathway Source: Reactome
  10. phagocytosis Source: ProtInc
  11. positive regulation of cytokine secretion Source: Ensembl
  12. positive regulation of endocytosis Source: Ensembl
  13. positive regulation of tumor necrosis factor production Source: MGI
  14. response to ethanol Source: Ensembl
  15. response to heat Source: Ensembl
  16. response to magnesium ion Source: Ensembl
  17. response to tumor necrosis factor Source: Ensembl
  18. toll-like receptor 2 signaling pathway Source: Reactome
  19. toll-like receptor 3 signaling pathway Source: Reactome
  20. toll-like receptor 4 signaling pathway Source: Reactome
  21. toll-like receptor signaling pathway Source: Reactome
  22. toll-like receptor TLR1:TLR2 signaling pathway Source: Reactome
  23. toll-like receptor TLR6:TLR2 signaling pathway Source: Reactome
  24. TRIF-dependent toll-like receptor signaling pathway Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

ReactomeiREACT_150361. TRIF-mediated programmed cell death.
REACT_25148. Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon.
REACT_25351. TRAF6 mediated induction of TAK1 complex.
REACT_25374. IKK complex recruitment mediated by RIP1.
REACT_6788. MyD88:Mal cascade initiated on plasma membrane.
REACT_6822. Transfer of LPS from LBP carrier to CD14.
REACT_6894. Toll Like Receptor 4 (TLR4) Cascade.
REACT_8005. Toll Like Receptor TLR1:TLR2 Cascade.
REACT_8006. Toll Like Receptor TLR6:TLR2 Cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Monocyte differentiation antigen CD14
Alternative name(s):
Myeloid cell-specific leucine-rich glycoprotein
CD_antigen: CD14
Cleaved into the following 2 chains:
Gene namesi
Name:CD14
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:1628. CD14.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. cell surface Source: Ensembl
  3. endosome membrane Source: Reactome
  4. extracellular region Source: Reactome
  5. extracellular space Source: UniProt
  6. extracellular vesicular exosome Source: UniProtKB
  7. membrane raft Source: Ensembl
  8. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26188.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Add
BLAST
Chaini20 – 367348Monocyte differentiation antigen CD14, urinary formPRO_0000020884Add
BLAST
Chaini20 – 345326Monocyte differentiation antigen CD14, membrane-bound formPRO_0000020885Add
BLAST
Propeptidei346 – 37530Removed in mature formSequence AnalysisPRO_0000020886Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi25 ↔ 361 Publication
Disulfide bondi34 ↔ 511 Publication
Glycosylationi37 – 371N-linked (GlcNAc...)Sequence Analysis
Glycosylationi151 – 1511N-linked (GlcNAc...)1 Publication
Disulfide bondi187 ↔ 2171 Publication
Disulfide bondi241 ↔ 2721 Publication
Glycosylationi282 – 2821N-linked (GlcNAc...)1 Publication
Glycosylationi323 – 3231N-linked (GlcNAc...)1 Publication
Glycosylationi336 – 3361O-linked (GalNAc...)1 Publication
Lipidationi345 – 3451GPI-anchor amidated asparagineSequence Analysis

Post-translational modificationi

N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.3 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiP08571.
PaxDbiP08571.
PeptideAtlasiP08571.
PRIDEiP08571.

PTM databases

PhosphoSiteiP08571.

Miscellaneous databases

PMAP-CutDBP08571.

Expressioni

Tissue specificityi

Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

Gene expression databases

BgeeiP08571.
CleanExiHS_CD14.
ExpressionAtlasiP08571. baseline and differential.
GenevestigatoriP08571.

Organism-specific databases

HPAiHPA001887.
HPA002127.

Interactioni

Subunit structurei

Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4.

Binary interactionsi

WithEntry#Exp.IntActNotes
FSTL1Q128413EBI-3905196,EBI-2349801

Protein-protein interaction databases

BioGridi107367. 9 interactions.
DIPiDIP-1030N.
IntActiP08571. 8 interactions.
MINTiMINT-7990664.
STRINGi9606.ENSP00000304236.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GLPX-ray4.00A26-335[»]
ProteinModelPortaliP08571.
SMRiP08571. Positions 26-335.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati54 – 8229LRR 1Add
BLAST
Repeati83 – 11836LRR 2Add
BLAST
Repeati119 – 14426LRR 3Add
BLAST
Repeati145 – 17228LRR 4Add
BLAST
Repeati173 – 19624LRR 5Add
BLAST
Repeati197 – 22428LRR 6Add
BLAST
Repeati225 – 25127LRR 7Add
BLAST
Repeati252 – 27827LRR 8Add
BLAST
Repeati279 – 29921LRR 9Add
BLAST
Repeati300 – 32122LRR 10Add
BLAST
Repeati322 – 34928LRR 11Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni42 – 8948Ligand-binding pocket rimAdd
BLAST

Sequence similaritiesi

Contains 11 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal

Phylogenomic databases

eggNOGiNOG86091.
GeneTreeiENSGT00390000005689.
HOGENOMiHOG000237268.
HOVERGENiHBG005269.
InParanoidiP08571.
KOiK04391.
OMAiWATGGAW.
OrthoDBiEOG751NFQ.
PhylomeDBiP08571.
TreeFamiTF338550.

Family and domain databases

InterProiIPR001611. Leu-rich_rpt.
IPR016337. Monocyte_diff_Ag_CD14.
[Graphical view]
PfamiPF00560. LRR_1. 1 hit.
[Graphical view]
PIRSFiPIRSF002017. CD14. 1 hit.
PROSITEiPS51450. LRR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08571-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MERASCLLLL LLPLVHVSAT TPEPCELDDE DFRCVCNFSE PQPDWSEAFQ
60 70 80 90 100
CVSAVEVEIH AGGLNLEPFL KRVDADADPR QYADTVKALR VRRLTVGAAQ
110 120 130 140 150
VPAQLLVGAL RVLAYSRLKE LTLEDLKITG TMPPLPLEAT GLALSSLRLR
160 170 180 190 200
NVSWATGRSW LAELQQWLKP GLKVLSIAQA HSPAFSCEQV RAFPALTSLD
210 220 230 240 250
LSDNPGLGER GLMAALCPHK FPAIQNLALR NTGMETPTGV CAALAAAGVQ
260 270 280 290 300
PHSLDLSHNS LRATVNPSAP RCMWSSALNS LNLSFAGLEQ VPKGLPAKLR
310 320 330 340 350
VLDLSCNRLN RAPQPDELPE VDNLTLDGNP FLVPGTALPH EGSMNSGVVP
360 370
ACARSTLSVG VSGTLVLLQG ARGFA
Length:375
Mass (Da):40,076
Last modified:March 27, 2002 - v2
Checksum:i1746CDB41F394F8D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti187 – 1871C → Y in CAA29999. (PubMed:2453848)Curated
Sequence conflicti303 – 3031D → E in AAC83816. 1 PublicationCurated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti204 – 2041N → D.
Corresponds to variant rs2228049 [ dbSNP | Ensembl ].
VAR_024302
Natural varianti341 – 3411E → K.
Corresponds to variant rs11556179 [ dbSNP | Ensembl ].
VAR_050771

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06882 Genomic DNA. Translation: CAA29999.1.
X13334 mRNA. Translation: CAA31711.1.
M86511 mRNA. Translation: AAA51930.1.
AF097942 mRNA. Translation: AAC83816.1.
AB446505 mRNA. Translation: BAG55282.1.
BT007331 mRNA. Translation: AAP35995.1.
CH471062 Genomic DNA. Translation: EAW62037.1.
BC010507 mRNA. Translation: AAH10507.1.
AY044269 mRNA. Translation: AAL02401.1.
CCDSiCCDS4232.1.
PIRiA27637. TDHUM4.
RefSeqiNP_000582.1. NM_000591.3.
NP_001035110.1. NM_001040021.2.
NP_001167575.1. NM_001174104.1.
NP_001167576.1. NM_001174105.1.
UniGeneiHs.163867.

Genome annotation databases

EnsembliENST00000302014; ENSP00000304236; ENSG00000170458.
ENST00000401743; ENSP00000385519; ENSG00000170458.
GeneIDi929.
KEGGihsa:929.
UCSCiuc003lgi.2. human.

Polymorphism databases

DMDMi20141203.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Wikipedia

CD14 entry

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06882 Genomic DNA. Translation: CAA29999.1 .
X13334 mRNA. Translation: CAA31711.1 .
M86511 mRNA. Translation: AAA51930.1 .
AF097942 mRNA. Translation: AAC83816.1 .
AB446505 mRNA. Translation: BAG55282.1 .
BT007331 mRNA. Translation: AAP35995.1 .
CH471062 Genomic DNA. Translation: EAW62037.1 .
BC010507 mRNA. Translation: AAH10507.1 .
AY044269 mRNA. Translation: AAL02401.1 .
CCDSi CCDS4232.1.
PIRi A27637. TDHUM4.
RefSeqi NP_000582.1. NM_000591.3.
NP_001035110.1. NM_001040021.2.
NP_001167575.1. NM_001174104.1.
NP_001167576.1. NM_001174105.1.
UniGenei Hs.163867.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4GLP X-ray 4.00 A 26-335 [» ]
ProteinModelPortali P08571.
SMRi P08571. Positions 26-335.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 107367. 9 interactions.
DIPi DIP-1030N.
IntActi P08571. 8 interactions.
MINTi MINT-7990664.
STRINGi 9606.ENSP00000304236.

Chemistry

ChEMBLi CHEMBL3038513.

PTM databases

PhosphoSitei P08571.

Polymorphism databases

DMDMi 20141203.

Proteomic databases

MaxQBi P08571.
PaxDbi P08571.
PeptideAtlasi P08571.
PRIDEi P08571.

Protocols and materials databases

DNASUi 929.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000302014 ; ENSP00000304236 ; ENSG00000170458 .
ENST00000401743 ; ENSP00000385519 ; ENSG00000170458 .
GeneIDi 929.
KEGGi hsa:929.
UCSCi uc003lgi.2. human.

Organism-specific databases

CTDi 929.
GeneCardsi GC05M139991.
HGNCi HGNC:1628. CD14.
HPAi HPA001887.
HPA002127.
MIMi 158120. gene.
neXtProti NX_P08571.
PharmGKBi PA26188.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG86091.
GeneTreei ENSGT00390000005689.
HOGENOMi HOG000237268.
HOVERGENi HBG005269.
InParanoidi P08571.
KOi K04391.
OMAi WATGGAW.
OrthoDBi EOG751NFQ.
PhylomeDBi P08571.
TreeFami TF338550.

Enzyme and pathway databases

Reactomei REACT_150361. TRIF-mediated programmed cell death.
REACT_25148. Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon.
REACT_25351. TRAF6 mediated induction of TAK1 complex.
REACT_25374. IKK complex recruitment mediated by RIP1.
REACT_6788. MyD88:Mal cascade initiated on plasma membrane.
REACT_6822. Transfer of LPS from LBP carrier to CD14.
REACT_6894. Toll Like Receptor 4 (TLR4) Cascade.
REACT_8005. Toll Like Receptor TLR1:TLR2 Cascade.
REACT_8006. Toll Like Receptor TLR6:TLR2 Cascade.

Miscellaneous databases

GeneWikii CD14.
GenomeRNAii 929.
NextBioi 3850.
PMAP-CutDB P08571.
PROi P08571.
SOURCEi Search...

Gene expression databases

Bgeei P08571.
CleanExi HS_CD14.
ExpressionAtlasi P08571. baseline and differential.
Genevestigatori P08571.

Family and domain databases

InterProi IPR001611. Leu-rich_rpt.
IPR016337. Monocyte_diff_Ag_CD14.
[Graphical view ]
Pfami PF00560. LRR_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF002017. CD14. 1 hit.
PROSITEi PS51450. LRR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The monocyte differentiation antigen, CD14, is anchored to the cell membrane by a phosphatidylinositol linkage."
    Haziot A., Chen S., Ferrero E., Low M.G., Silber R., Goyert S.M.
    J. Immunol. 141:547-552(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Nucleotide sequence of the gene encoding the monocyte differentiation antigen, CD14."
    Ferrero E., Goyert S.M.
    Nucleic Acids Res. 16:4173-4173(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Lymphocyte.
  3. "Mouse and human CD14 (myeloid cell-specific leucine-rich glycoprotein) primary structure deduced from cDNA clones."
    Setoguchi M., Nasu N., Yoshida S., Higuchi Y., Akizuki S., Yamamoto S.
    Biochim. Biophys. Acta 1008:213-222(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Macrophage.
  4. "Monocyte antigen CD14 is a phospholipid anchored membrane protein."
    Simmons D.L., Tan S., Tenen D.G., Nicholson-Weller A., Seed B.
    Blood 73:284-289(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "Cloning and sequencing of human CD14 gene."
    Long J.Y., Xue Y.N., Sun L., Wang H.X.
    Sheng Wu Hua Xue Yu Sheng Wu Wu Li Jin Zhan 25:377-378(1998)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Promyelocytic leukemia.
  6. "Natural selection in the TLR-related genes in the course of primate evolution."
    Nakajima T., Ohtani H., Satta Y., Uno Y., Akari H., Ishida T., Kimura A.
    Immunogenetics 60:727-735(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  7. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  10. "Expression and secretion of CD14 in glial neoplasms of the brain."
    Deininger M.H., Meyermann R., Schluesener H.J.
    Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-125.
    Tissue: Glioblastoma.
  11. "Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14."
    Bazil V., Baudys M., Hilgert I., Stefanova I., Low M.G., Zbrozek J., Horejsi V.
    Mol. Immunol. 26:657-662(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 362-367.
  12. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
    Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
    J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-151 AND ASN-282.
    Tissue: Plasma.
  13. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
    Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
    J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-323.
    Tissue: Liver.
  14. "Enrichment of glycopeptides for glycan structure and attachment site identification."
    Nilsson J., Rueetschi U., Halim A., Hesse C., Carlsohn E., Brinkmalm G., Larson G.
    Nat. Methods 6:809-811(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT THR-336, STRUCTURE OF CARBOHYDRATES.
    Tissue: Cerebrospinal fluid.
  15. "The crystal structure of human soluble CD14 reveals a bent solenoid with a hydrophobic amino-terminal pocket."
    Kelley S.L., Lukk T., Nair S.K., Tapping R.I.
    J. Immunol. 190:1304-1311(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 26-335, DISULFIDE BONDS, FUNCTION.

Entry informationi

Entry nameiCD14_HUMAN
AccessioniPrimary (citable) accession number: P08571
Secondary accession number(s): Q53XT5
, Q96FR6, Q96L99, Q9UNS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: March 27, 2002
Last modified: October 29, 2014
This is version 159 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3