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Protein

Monocyte differentiation antigen CD14

Gene

CD14

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- (PubMed:23880187).6 Publications

GO - Molecular functioni

  • lipopolysaccharide binding Source: MGI
  • lipoteichoic acid binding Source: MGI
  • opsonin receptor activity Source: BHF-UCL
  • peptidoglycan receptor activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

ReactomeiR-HSA-140534. Ligand-dependent caspase activation.
R-HSA-166016. Toll Like Receptor 4 (TLR4) Cascade.
R-HSA-166020. Transfer of LPS from LBP carrier to CD14.
R-HSA-166058. MyD88:Mal cascade initiated on plasma membrane.
R-HSA-166166. MyD88-independent TLR3/TLR4 cascade.
R-HSA-168179. Toll Like Receptor TLR1:TLR2 Cascade.
R-HSA-168188. Toll Like Receptor TLR6:TLR2 Cascade.
R-HSA-2562578. TRIF-mediated programmed cell death.
R-HSA-5602498. MyD88 deficiency (TLR2/4).
R-HSA-5603041. IRAK4 deficiency (TLR2/4).
R-HSA-936964. Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon.
R-HSA-937041. IKK complex recruitment mediated by RIP1.
R-HSA-937072. TRAF6 mediated induction of TAK1 complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Monocyte differentiation antigen CD141 Publication
Alternative name(s):
Myeloid cell-specific leucine-rich glycoprotein
CD_antigen: CD14
Cleaved into the following 2 chains:
Gene namesi
Name:CD14
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:1628. CD14.

Subcellular locationi

GO - Cellular componenti

  • anchored component of external side of plasma membrane Source: UniProtKB
  • endosome membrane Source: Reactome
  • extracellular exosome Source: UniProtKB
  • extracellular region Source: Reactome
  • extracellular space Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • lipopolysaccharide receptor complex Source: UniProtKB
  • membrane raft Source: UniProtKB
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26188.

Chemistry

ChEMBLiCHEMBL3038513.

Polymorphism and mutation databases

BioMutaiCD14.
DMDMi20141203.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Add
BLAST
Chaini20 – 367348Monocyte differentiation antigen CD14, urinary formPRO_0000020884Add
BLAST
Chaini20 – 345326Monocyte differentiation antigen CD14, membrane-bound formPRO_0000020885Add
BLAST
Propeptidei346 – 37530Removed in mature formSequence analysisPRO_0000020886Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi25 ↔ 361 Publication
Disulfide bondi34 ↔ 511 Publication
Glycosylationi37 – 371N-linked (GlcNAc...)Sequence analysis
Glycosylationi151 – 1511N-linked (GlcNAc...)1 Publication
Disulfide bondi187 ↔ 2171 Publication
Disulfide bondi241 ↔ 2721 Publication
Glycosylationi282 – 2821N-linked (GlcNAc...)1 Publication
Glycosylationi323 – 3231N-linked (GlcNAc...)1 Publication
Glycosylationi336 – 3361O-linked (GalNAc...)1 Publication
Lipidationi345 – 3451GPI-anchor amidated asparagineSequence analysis

Post-translational modificationi

N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.3 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiP08571.
PaxDbiP08571.
PeptideAtlasiP08571.
PRIDEiP08571.

PTM databases

iPTMnetiP08571.
PhosphoSiteiP08571.

Miscellaneous databases

PMAP-CutDBP08571.

Expressioni

Tissue specificityi

Detected on macrophages (at protein level) (PubMed:1698311). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.2 Publications

Inductioni

The expression in monocytes is highly induced by 27-hydroxycholesterol, priming monocytes/macrophages such that LPS-mediated inflammatory reaction is accelerated. Secretion of soluble CD14 is also enhanced.1 Publication

Gene expression databases

BgeeiENSG00000170458.
CleanExiHS_CD14.
ExpressionAtlasiP08571. baseline and differential.
GenevisibleiP08571. HS.

Organism-specific databases

HPAiCAB033631.
CAB072865.
CAB072866.
HPA001887.
HPA002127.

Interactioni

Subunit structurei

Interacts with LPS-bound LPB (PubMed:1698311, PubMed:23264655). Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4 (PubMed:11274165). Interacts with LPAR1 (By similarity). Interacts with the TLR2:TLR6 or TLR2:TLR1 heterodimers; upon interaction with ligands such as diacylated lipopeptides and triacylated lipopeptides, respectively (PubMed:16880211).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
FSTL1Q128413EBI-3905196,EBI-2349801

Protein-protein interaction databases

BioGridi107367. 25 interactions.
DIPiDIP-1030N.
IntActiP08571. 24 interactions.
MINTiMINT-7990664.
STRINGi9606.ENSP00000304236.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GLPX-ray4.00A26-335[»]
ProteinModelPortaliP08571.
SMRiP08571. Positions 26-335.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati54 – 8229LRR 1Add
BLAST
Repeati83 – 11836LRR 2Add
BLAST
Repeati119 – 14426LRR 3Add
BLAST
Repeati145 – 17228LRR 4Add
BLAST
Repeati173 – 19624LRR 5Add
BLAST
Repeati197 – 22428LRR 6Add
BLAST
Repeati225 – 25127LRR 7Add
BLAST
Repeati252 – 27827LRR 8Add
BLAST
Repeati279 – 29921LRR 9Add
BLAST
Repeati300 – 32122LRR 10Add
BLAST
Repeati322 – 34928LRR 11Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni290 – 37586Required for response to bacterial lipopolysaccharide (LPS)By similarityAdd
BLAST

Domaini

The C-terminal leucine-rich repeat (LRR) region is required for responses to smooth LPS.By similarity

Sequence similaritiesi

Contains 11 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IXG9. Eukaryota.
ENOG41118N2. LUCA.
GeneTreeiENSGT00390000005689.
HOGENOMiHOG000237268.
HOVERGENiHBG005269.
InParanoidiP08571.
KOiK04391.
OMAiLCPHKFP.
OrthoDBiEOG091G0DA3.
PhylomeDBiP08571.
TreeFamiTF338550.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR016337. Monocyte_diff_Ag_CD14.
[Graphical view]
PIRSFiPIRSF002017. CD14. 1 hit.
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08571-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERASCLLLL LLPLVHVSAT TPEPCELDDE DFRCVCNFSE PQPDWSEAFQ
60 70 80 90 100
CVSAVEVEIH AGGLNLEPFL KRVDADADPR QYADTVKALR VRRLTVGAAQ
110 120 130 140 150
VPAQLLVGAL RVLAYSRLKE LTLEDLKITG TMPPLPLEAT GLALSSLRLR
160 170 180 190 200
NVSWATGRSW LAELQQWLKP GLKVLSIAQA HSPAFSCEQV RAFPALTSLD
210 220 230 240 250
LSDNPGLGER GLMAALCPHK FPAIQNLALR NTGMETPTGV CAALAAAGVQ
260 270 280 290 300
PHSLDLSHNS LRATVNPSAP RCMWSSALNS LNLSFAGLEQ VPKGLPAKLR
310 320 330 340 350
VLDLSCNRLN RAPQPDELPE VDNLTLDGNP FLVPGTALPH EGSMNSGVVP
360 370
ACARSTLSVG VSGTLVLLQG ARGFA
Length:375
Mass (Da):40,076
Last modified:March 27, 2002 - v2
Checksum:i1746CDB41F394F8D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti187 – 1871C → Y in CAA29999 (PubMed:2453848).Curated
Sequence conflicti303 – 3031D → E in AAC83816 (Ref. 5) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti204 – 2041N → D.
Corresponds to variant rs2228049 [ dbSNP | Ensembl ].
VAR_024302
Natural varianti341 – 3411E → K.
Corresponds to variant rs11556179 [ dbSNP | Ensembl ].
VAR_050771

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06882 Genomic DNA. Translation: CAA29999.1.
X13334 mRNA. Translation: CAA31711.1.
M86511 mRNA. Translation: AAA51930.1.
AF097942 mRNA. Translation: AAC83816.1.
AB446505 mRNA. Translation: BAG55282.1.
BT007331 mRNA. Translation: AAP35995.1.
CH471062 Genomic DNA. Translation: EAW62037.1.
BC010507 mRNA. Translation: AAH10507.1.
AY044269 mRNA. Translation: AAL02401.1.
CCDSiCCDS4232.1.
PIRiA27637. TDHUM4.
RefSeqiNP_000582.1. NM_000591.3.
NP_001035110.1. NM_001040021.2.
NP_001167575.1. NM_001174104.1.
NP_001167576.1. NM_001174105.1.
UniGeneiHs.163867.

Genome annotation databases

EnsembliENST00000302014; ENSP00000304236; ENSG00000170458.
ENST00000401743; ENSP00000385519; ENSG00000170458.
GeneIDi929.
KEGGihsa:929.
UCSCiuc003lgi.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Wikipedia

CD14 entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06882 Genomic DNA. Translation: CAA29999.1.
X13334 mRNA. Translation: CAA31711.1.
M86511 mRNA. Translation: AAA51930.1.
AF097942 mRNA. Translation: AAC83816.1.
AB446505 mRNA. Translation: BAG55282.1.
BT007331 mRNA. Translation: AAP35995.1.
CH471062 Genomic DNA. Translation: EAW62037.1.
BC010507 mRNA. Translation: AAH10507.1.
AY044269 mRNA. Translation: AAL02401.1.
CCDSiCCDS4232.1.
PIRiA27637. TDHUM4.
RefSeqiNP_000582.1. NM_000591.3.
NP_001035110.1. NM_001040021.2.
NP_001167575.1. NM_001174104.1.
NP_001167576.1. NM_001174105.1.
UniGeneiHs.163867.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GLPX-ray4.00A26-335[»]
ProteinModelPortaliP08571.
SMRiP08571. Positions 26-335.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107367. 25 interactions.
DIPiDIP-1030N.
IntActiP08571. 24 interactions.
MINTiMINT-7990664.
STRINGi9606.ENSP00000304236.

Chemistry

ChEMBLiCHEMBL3038513.

PTM databases

iPTMnetiP08571.
PhosphoSiteiP08571.

Polymorphism and mutation databases

BioMutaiCD14.
DMDMi20141203.

Proteomic databases

MaxQBiP08571.
PaxDbiP08571.
PeptideAtlasiP08571.
PRIDEiP08571.

Protocols and materials databases

DNASUi929.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000302014; ENSP00000304236; ENSG00000170458.
ENST00000401743; ENSP00000385519; ENSG00000170458.
GeneIDi929.
KEGGihsa:929.
UCSCiuc003lgi.3. human.

Organism-specific databases

CTDi929.
GeneCardsiCD14.
HGNCiHGNC:1628. CD14.
HPAiCAB033631.
CAB072865.
CAB072866.
HPA001887.
HPA002127.
MIMi158120. gene.
neXtProtiNX_P08571.
PharmGKBiPA26188.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IXG9. Eukaryota.
ENOG41118N2. LUCA.
GeneTreeiENSGT00390000005689.
HOGENOMiHOG000237268.
HOVERGENiHBG005269.
InParanoidiP08571.
KOiK04391.
OMAiLCPHKFP.
OrthoDBiEOG091G0DA3.
PhylomeDBiP08571.
TreeFamiTF338550.

Enzyme and pathway databases

ReactomeiR-HSA-140534. Ligand-dependent caspase activation.
R-HSA-166016. Toll Like Receptor 4 (TLR4) Cascade.
R-HSA-166020. Transfer of LPS from LBP carrier to CD14.
R-HSA-166058. MyD88:Mal cascade initiated on plasma membrane.
R-HSA-166166. MyD88-independent TLR3/TLR4 cascade.
R-HSA-168179. Toll Like Receptor TLR1:TLR2 Cascade.
R-HSA-168188. Toll Like Receptor TLR6:TLR2 Cascade.
R-HSA-2562578. TRIF-mediated programmed cell death.
R-HSA-5602498. MyD88 deficiency (TLR2/4).
R-HSA-5603041. IRAK4 deficiency (TLR2/4).
R-HSA-936964. Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon.
R-HSA-937041. IKK complex recruitment mediated by RIP1.
R-HSA-937072. TRAF6 mediated induction of TAK1 complex.

Miscellaneous databases

ChiTaRSiCD14. human.
GeneWikiiCD14.
GenomeRNAii929.
PMAP-CutDBP08571.
PROiP08571.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000170458.
CleanExiHS_CD14.
ExpressionAtlasiP08571. baseline and differential.
GenevisibleiP08571. HS.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR016337. Monocyte_diff_Ag_CD14.
[Graphical view]
PIRSFiPIRSF002017. CD14. 1 hit.
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCD14_HUMAN
AccessioniPrimary (citable) accession number: P08571
Secondary accession number(s): Q53XT5
, Q96FR6, Q96L99, Q9UNS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: March 27, 2002
Last modified: September 7, 2016
This is version 177 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.