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Protein

Pleckstrin

Gene

PLEK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major protein kinase C substrate of platelets.

GO - Molecular functioni

  • phosphatidylinositol-3,4-bisphosphate binding Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL
  • protein kinase C binding Source: BHF-UCL

GO - Biological processi

  • actin cytoskeleton reorganization Source: BHF-UCL
  • cell projection organization Source: BHF-UCL
  • cortical actin cytoskeleton organization Source: BHF-UCL
  • hematopoietic progenitor cell differentiation Source: BHF-UCL
  • integrin-mediated signaling pathway Source: BHF-UCL
  • negative regulation of calcium-mediated signaling Source: BHF-UCL
  • negative regulation of G-protein coupled receptor protein signaling pathway Source: BHF-UCL
  • negative regulation of inositol phosphate biosynthetic process Source: BHF-UCL
  • phosphatidylinositol metabolic process Source: BHF-UCL
  • phospholipase C-inhibiting G-protein coupled receptor signaling pathway Source: BHF-UCL
  • platelet aggregation Source: UniProtKB
  • platelet degranulation Source: BHF-UCL
  • positive regulation of actin filament bundle assembly Source: BHF-UCL
  • positive regulation of actin filament depolymerization Source: BHF-UCL
  • positive regulation of inositol-polyphosphate 5-phosphatase activity Source: BHF-UCL
  • positive regulation of integrin activation Source: BHF-UCL
  • positive regulation of platelet activation Source: BHF-UCL
  • protein kinase C signaling Source: BHF-UCL
  • protein secretion by platelet Source: BHF-UCL
  • regulation of cell diameter Source: BHF-UCL
  • ruffle organization Source: BHF-UCL
  • thrombin-activated receptor signaling pathway Source: BHF-UCL
  • vesicle docking involved in exocytosis Source: BHF-UCL
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000115956-MONOMER.
ReactomeiR-HSA-114608. Platelet degranulation.
SIGNORiP08567.

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin
Alternative name(s):
Platelet 47 kDa protein
Short name:
p47
Gene namesi
Name:PLEK
Synonyms:P47
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:9070. PLEK.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • cytosol Source: Reactome
  • extracellular region Source: Reactome
  • membrane Source: UniProtKB
  • ruffle membrane Source: BHF-UCL
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi5341.
OpenTargetsiENSG00000115956.
PharmGKBiPA33400.

Polymorphism and mutation databases

BioMutaiPLEK.
DMDMi317373523.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000538591 – 350PleckstrinAdd BLAST350

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei64N6-acetyllysineCombined sources1
Modified residuei113Phosphoserine; by PKC1 Publication1
Modified residuei117Phosphoserine; by PKCCombined sources1 Publication1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP08567.
MaxQBiP08567.
PaxDbiP08567.
PeptideAtlasiP08567.
PRIDEiP08567.

2D gel databases

OGPiP08567.

PTM databases

iPTMnetiP08567.
PhosphoSitePlusiP08567.

Expressioni

Gene expression databases

BgeeiENSG00000115956.
CleanExiHS_PLEK.
GenevisibleiP08567. HS.

Organism-specific databases

HPAiHPA031838.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
INPP5AQ146424EBI-2565501,EBI-8670520
SDPRO958104EBI-2565501,EBI-742141

GO - Molecular functioni

  • protein homodimerization activity Source: BHF-UCL
  • protein kinase C binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi111357. 2 interactors.
IntActiP08567. 10 interactors.
MINTiMINT-8216753.
STRINGi9606.ENSP00000234313.

Structurei

Secondary structure

1350
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 19Combined sources14
Beta strandi22 – 29Combined sources8
Beta strandi32 – 38Combined sources7
Beta strandi45 – 52Combined sources8
Beta strandi55 – 58Combined sources4
Beta strandi65 – 73Combined sources9
Turni74 – 76Combined sources3
Beta strandi77 – 82Combined sources6
Helixi86 – 103Combined sources18
Helixi126 – 133Combined sources8
Beta strandi135 – 140Combined sources6
Beta strandi143 – 147Combined sources5
Beta strandi155 – 157Combined sources3
Helixi158 – 167Combined sources10
Beta strandi168 – 170Combined sources3
Helixi174 – 187Combined sources14
Beta strandi189 – 194Combined sources6
Helixi195 – 200Combined sources6
Beta strandi215 – 218Combined sources4
Beta strandi220 – 222Combined sources3
Beta strandi226 – 228Combined sources3
Helixi239 – 241Combined sources3
Beta strandi245 – 254Combined sources10
Turni256 – 258Combined sources3
Beta strandi261 – 269Combined sources9
Turni270 – 273Combined sources4
Beta strandi274 – 278Combined sources5
Beta strandi280 – 282Combined sources3
Beta strandi287 – 291Combined sources5
Beta strandi296 – 300Combined sources5
Beta strandi306 – 308Combined sources3
Beta strandi313 – 318Combined sources6
Beta strandi324 – 328Combined sources5
Helixi332 – 346Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PLSNMR-A1-105[»]
1W4MNMR-A121-223[»]
1X05NMR-A235-350[»]
1XX0NMR-A234-350[»]
1ZM0X-ray2.10A/B240-350[»]
2CSONMR-A116-229[»]
2I5CX-ray1.75A/B/C244-347[»]
2I5FX-ray1.35A244-347[»]
ProteinModelPortaliP08567.
SMRiP08567.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08567.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 101PH 1PROSITE-ProRule annotationAdd BLAST98
Domaini136 – 221DEPPROSITE-ProRule annotationAdd BLAST86
Domaini244 – 347PH 2PROSITE-ProRule annotationAdd BLAST104

Sequence similaritiesi

Contains 1 DEP domain.PROSITE-ProRule annotation
Contains 2 PH domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IFJ7. Eukaryota.
ENOG410ZY9B. LUCA.
GeneTreeiENSGT00390000006543.
HOGENOMiHOG000294078.
HOVERGENiHBG001361.
InParanoidiP08567.
KOiK19993.
OMAiFCEGYSS.
OrthoDBiEOG091G09EY.
PhylomeDBiP08567.
TreeFamiTF332246.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.30.29.30. 2 hits.
InterProiIPR000591. DEP_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00610. DEP. 1 hit.
PF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00049. DEP. 1 hit.
SM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50729. SSF50729. 2 hits.
PROSITEiPS50186. DEP. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08567-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPKRIREGY LVKKGSVFNT WKPMWVVLLE DGIEFYKKKS DNSPKGMIPL
60 70 80 90 100
KGSTLTSPCQ DFGKRMFVFK ITTTKQQDHF FQAAFLEERD AWVRDIKKAI
110 120 130 140 150
KCIEGGQKFA RKSTRRSIRL PETIDLGALY LSMKDTEKGI KELNLEKDKK
160 170 180 190 200
IFNHCFTGNC VIDWLVSNQS VRNRQEGLMI ASSLLNEGYL QPAGDMSKSA
210 220 230 240 250
VDGTAENPFL DNPDAFYYFP DSGFFCEENS SDDDVILKEE FRGVIIKQGC
260 270 280 290 300
LLKQGHRRKN WKVRKFILRE DPAYLHYYDP AGAEDPLGAI HLRGCVVTSV
310 320 330 340 350
ESNSNGRKSE EENLFEIITA DEVHYFLQAA TPKERTEWIR AIQMASRTGK
Length:350
Mass (Da):40,125
Last modified:January 11, 2011 - v3
Checksum:i2E292CB18B533261
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti284E → G in CAG46876 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0277975R → W.Corresponds to variant rs17035364dbSNPEnsembl.1
Natural variantiVAR_00552492W → R.1
Natural variantiVAR_02779897K → N.2 PublicationsCorresponds to variant rs3816281dbSNPEnsembl.1
Natural variantiVAR_056666108K → Q.Corresponds to variant rs34515106dbSNPEnsembl.1
Natural variantiVAR_027799340R → K.4 PublicationsCorresponds to variant rs1063479dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07743 mRNA. Translation: CAA30564.1.
CR542056 mRNA. Translation: CAG46853.1.
CR542079 mRNA. Translation: CAG46876.1.
AK313756 mRNA. Translation: BAG36495.1.
AC015969 Genomic DNA. Translation: AAX93121.1.
CH471053 Genomic DNA. Translation: EAW99876.1.
BC018549 mRNA. Translation: AAH18549.1.
CCDSiCCDS1887.1.
PIRiS00755.
RefSeqiNP_002655.2. NM_002664.2.
UniGeneiHs.468840.

Genome annotation databases

EnsembliENST00000234313; ENSP00000234313; ENSG00000115956.
GeneIDi5341.
KEGGihsa:5341.
UCSCiuc002sen.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07743 mRNA. Translation: CAA30564.1.
CR542056 mRNA. Translation: CAG46853.1.
CR542079 mRNA. Translation: CAG46876.1.
AK313756 mRNA. Translation: BAG36495.1.
AC015969 Genomic DNA. Translation: AAX93121.1.
CH471053 Genomic DNA. Translation: EAW99876.1.
BC018549 mRNA. Translation: AAH18549.1.
CCDSiCCDS1887.1.
PIRiS00755.
RefSeqiNP_002655.2. NM_002664.2.
UniGeneiHs.468840.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PLSNMR-A1-105[»]
1W4MNMR-A121-223[»]
1X05NMR-A235-350[»]
1XX0NMR-A234-350[»]
1ZM0X-ray2.10A/B240-350[»]
2CSONMR-A116-229[»]
2I5CX-ray1.75A/B/C244-347[»]
2I5FX-ray1.35A244-347[»]
ProteinModelPortaliP08567.
SMRiP08567.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111357. 2 interactors.
IntActiP08567. 10 interactors.
MINTiMINT-8216753.
STRINGi9606.ENSP00000234313.

PTM databases

iPTMnetiP08567.
PhosphoSitePlusiP08567.

Polymorphism and mutation databases

BioMutaiPLEK.
DMDMi317373523.

2D gel databases

OGPiP08567.

Proteomic databases

EPDiP08567.
MaxQBiP08567.
PaxDbiP08567.
PeptideAtlasiP08567.
PRIDEiP08567.

Protocols and materials databases

DNASUi5341.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000234313; ENSP00000234313; ENSG00000115956.
GeneIDi5341.
KEGGihsa:5341.
UCSCiuc002sen.5. human.

Organism-specific databases

CTDi5341.
DisGeNETi5341.
GeneCardsiPLEK.
H-InvDBHIX0002122.
HGNCiHGNC:9070. PLEK.
HPAiHPA031838.
MIMi173570. gene.
neXtProtiNX_P08567.
OpenTargetsiENSG00000115956.
PharmGKBiPA33400.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFJ7. Eukaryota.
ENOG410ZY9B. LUCA.
GeneTreeiENSGT00390000006543.
HOGENOMiHOG000294078.
HOVERGENiHBG001361.
InParanoidiP08567.
KOiK19993.
OMAiFCEGYSS.
OrthoDBiEOG091G09EY.
PhylomeDBiP08567.
TreeFamiTF332246.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000115956-MONOMER.
ReactomeiR-HSA-114608. Platelet degranulation.
SIGNORiP08567.

Miscellaneous databases

ChiTaRSiPLEK. human.
EvolutionaryTraceiP08567.
GenomeRNAii5341.
PROiP08567.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000115956.
CleanExiHS_PLEK.
GenevisibleiP08567. HS.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.30.29.30. 2 hits.
InterProiIPR000591. DEP_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00610. DEP. 1 hit.
PF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00049. DEP. 1 hit.
SM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50729. SSF50729. 2 hits.
PROSITEiPS50186. DEP. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLEK_HUMAN
AccessioniPrimary (citable) accession number: P08567
Secondary accession number(s): B2R9E8
, Q53SU8, Q6FGM8, Q6FGQ1, Q8WV81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 11, 2011
Last modified: November 30, 2016
This is version 180 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.