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Protein

Pleckstrin

Gene

PLEK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Major protein kinase C substrate of platelets.

GO - Molecular functioni

  • phosphatidylinositol-3,4-bisphosphate binding Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL
  • protein kinase C binding Source: BHF-UCL
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: NTNU_SB

GO - Biological processi

  • actin cytoskeleton reorganization Source: BHF-UCL
  • cell projection organization Source: BHF-UCL
  • cortical actin cytoskeleton organization Source: BHF-UCL
  • hematopoietic progenitor cell differentiation Source: BHF-UCL
  • integrin-mediated signaling pathway Source: BHF-UCL
  • negative regulation of calcium-mediated signaling Source: BHF-UCL
  • negative regulation of G-protein coupled receptor protein signaling pathway Source: BHF-UCL
  • negative regulation of inositol phosphate biosynthetic process Source: BHF-UCL
  • phosphatidylinositol metabolic process Source: BHF-UCL
  • phospholipase C-inhibiting G-protein coupled receptor signaling pathway Source: BHF-UCL
  • platelet aggregation Source: UniProtKB
  • platelet degranulation Source: BHF-UCL
  • positive regulation of actin filament bundle assembly Source: BHF-UCL
  • positive regulation of actin filament depolymerization Source: BHF-UCL
  • positive regulation of inositol-polyphosphate 5-phosphatase activity Source: BHF-UCL
  • positive regulation of integrin activation Source: BHF-UCL
  • positive regulation of platelet activation Source: BHF-UCL
  • protein kinase C signaling Source: BHF-UCL
  • protein secretion by platelet Source: BHF-UCL
  • regulation of cell diameter Source: BHF-UCL
  • ruffle organization Source: BHF-UCL
  • thrombin-activated receptor signaling pathway Source: BHF-UCL
  • vesicle docking involved in exocytosis Source: BHF-UCL

Enzyme and pathway databases

ReactomeiR-HSA-114608 Platelet degranulation
SIGNORiP08567

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin
Alternative name(s):
Platelet 47 kDa protein
Short name:
p47
Gene namesi
Name:PLEK
Synonyms:P47
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000115956.9
HGNCiHGNC:9070 PLEK
MIMi173570 gene
neXtProtiNX_P08567

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi5341
OpenTargetsiENSG00000115956
PharmGKBiPA33400

Polymorphism and mutation databases

BioMutaiPLEK
DMDMi317373523

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000538591 – 350PleckstrinAdd BLAST350

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei64N6-acetyllysineCombined sources1
Modified residuei113Phosphoserine; by PKC1 Publication1
Modified residuei117Phosphoserine; by PKCCombined sources1 Publication1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP08567
MaxQBiP08567
PaxDbiP08567
PeptideAtlasiP08567
PRIDEiP08567

2D gel databases

OGPiP08567

PTM databases

iPTMnetiP08567
PhosphoSitePlusiP08567

Expressioni

Gene expression databases

BgeeiENSG00000115956
CleanExiHS_PLEK
GenevisibleiP08567 HS

Organism-specific databases

HPAiHPA031838

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein homodimerization activity Source: BHF-UCL
  • protein kinase C binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi1113572 interactors.
IntActiP08567 10 interactors.
MINTiP08567
STRINGi9606.ENSP00000234313

Structurei

Secondary structure

1350
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 19Combined sources14
Beta strandi22 – 29Combined sources8
Beta strandi32 – 38Combined sources7
Beta strandi45 – 52Combined sources8
Beta strandi55 – 58Combined sources4
Beta strandi65 – 73Combined sources9
Turni74 – 76Combined sources3
Beta strandi77 – 82Combined sources6
Helixi86 – 103Combined sources18
Helixi126 – 133Combined sources8
Beta strandi135 – 140Combined sources6
Beta strandi143 – 147Combined sources5
Beta strandi155 – 157Combined sources3
Helixi158 – 167Combined sources10
Beta strandi168 – 170Combined sources3
Helixi174 – 187Combined sources14
Beta strandi189 – 194Combined sources6
Helixi195 – 200Combined sources6
Beta strandi215 – 218Combined sources4
Beta strandi220 – 222Combined sources3
Beta strandi226 – 228Combined sources3
Helixi239 – 241Combined sources3
Beta strandi245 – 254Combined sources10
Turni256 – 258Combined sources3
Beta strandi261 – 269Combined sources9
Turni270 – 273Combined sources4
Beta strandi274 – 278Combined sources5
Beta strandi280 – 282Combined sources3
Beta strandi287 – 291Combined sources5
Beta strandi296 – 300Combined sources5
Beta strandi306 – 308Combined sources3
Beta strandi313 – 318Combined sources6
Beta strandi324 – 328Combined sources5
Helixi332 – 346Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PLSNMR-A1-105[»]
1W4MNMR-A121-223[»]
1X05NMR-A235-350[»]
1XX0NMR-A234-350[»]
1ZM0X-ray2.10A/B240-350[»]
2CSONMR-A116-229[»]
2I5CX-ray1.75A/B/C244-347[»]
2I5FX-ray1.35A244-347[»]
ProteinModelPortaliP08567
SMRiP08567
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08567

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 101PH 1PROSITE-ProRule annotationAdd BLAST98
Domaini136 – 221DEPPROSITE-ProRule annotationAdd BLAST86
Domaini244 – 347PH 2PROSITE-ProRule annotationAdd BLAST104

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IFJ7 Eukaryota
ENOG410ZY9B LUCA
GeneTreeiENSGT00390000006543
HOGENOMiHOG000294078
HOVERGENiHBG001361
InParanoidiP08567
KOiK19993
OMAiFCEGYSS
OrthoDBiEOG091G09EY
PhylomeDBiP08567
TreeFamiTF332246

Family and domain databases

CDDicd04445 DEP_PLEK1, 1 hit
Gene3Di1.10.10.101 hit
2.30.29.302 hits
InterProiView protein in InterPro
IPR000591 DEP_dom
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR037370 Pleckstrin
IPR037371 PLEK1_DEP
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12092 PTHR12092, 1 hit
PfamiView protein in Pfam
PF00610 DEP, 1 hit
PF00169 PH, 2 hits
SMARTiView protein in SMART
SM00049 DEP, 1 hit
SM00233 PH, 2 hits
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS50186 DEP, 1 hit
PS50003 PH_DOMAIN, 2 hits

Sequencei

Sequence statusi: Complete.

P08567-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPKRIREGY LVKKGSVFNT WKPMWVVLLE DGIEFYKKKS DNSPKGMIPL
60 70 80 90 100
KGSTLTSPCQ DFGKRMFVFK ITTTKQQDHF FQAAFLEERD AWVRDIKKAI
110 120 130 140 150
KCIEGGQKFA RKSTRRSIRL PETIDLGALY LSMKDTEKGI KELNLEKDKK
160 170 180 190 200
IFNHCFTGNC VIDWLVSNQS VRNRQEGLMI ASSLLNEGYL QPAGDMSKSA
210 220 230 240 250
VDGTAENPFL DNPDAFYYFP DSGFFCEENS SDDDVILKEE FRGVIIKQGC
260 270 280 290 300
LLKQGHRRKN WKVRKFILRE DPAYLHYYDP AGAEDPLGAI HLRGCVVTSV
310 320 330 340 350
ESNSNGRKSE EENLFEIITA DEVHYFLQAA TPKERTEWIR AIQMASRTGK
Length:350
Mass (Da):40,125
Last modified:January 11, 2011 - v3
Checksum:i2E292CB18B533261
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti284E → G in CAG46876 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0277975R → W. Corresponds to variant dbSNP:rs17035364Ensembl.1
Natural variantiVAR_00552492W → R. 1
Natural variantiVAR_02779897K → N2 PublicationsCorresponds to variant dbSNP:rs3816281Ensembl.1
Natural variantiVAR_056666108K → Q. Corresponds to variant dbSNP:rs34515106Ensembl.1
Natural variantiVAR_027799340R → K4 PublicationsCorresponds to variant dbSNP:rs1063479Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07743 mRNA Translation: CAA30564.1
CR542056 mRNA Translation: CAG46853.1
CR542079 mRNA Translation: CAG46876.1
AK313756 mRNA Translation: BAG36495.1
AC015969 Genomic DNA Translation: AAX93121.1
CH471053 Genomic DNA Translation: EAW99876.1
BC018549 mRNA Translation: AAH18549.1
CCDSiCCDS1887.1
PIRiS00755
RefSeqiNP_002655.2, NM_002664.2
UniGeneiHs.468840

Genome annotation databases

EnsembliENST00000234313; ENSP00000234313; ENSG00000115956
GeneIDi5341
KEGGihsa:5341
UCSCiuc002sen.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPLEK_HUMAN
AccessioniPrimary (citable) accession number: P08567
Secondary accession number(s): B2R9E8
, Q53SU8, Q6FGM8, Q6FGQ1, Q8WV81
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 11, 2011
Last modified: March 28, 2018
This is version 188 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome