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Protein

Pentafunctional AROM polypeptide

Gene

ARO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The AROM polypeptide catalyzes 5 consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis.

Miscellaneous

Present with 6420 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate = 3-dehydroquinate + phosphate.UniRule annotation
3-dehydroquinate = 3-dehydroshikimate + H2O.UniRule annotation
Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation
ATP + shikimate = ADP + shikimate 3-phosphate.UniRule annotation
Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 2, 3, 4, 5 and 6 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase, tyrosine-inhibited (ARO4), Phospho-2-dehydro-3-deoxyheptonate aldolase, phenylalanine-inhibited (ARO3)
  2. Pentafunctional AROM polypeptide (ARO1)
  3. Pentafunctional AROM polypeptide (ARO1)
  4. Pentafunctional AROM polypeptide (ARO1)
  5. Pentafunctional AROM polypeptide (ARO1)
  6. Pentafunctional AROM polypeptide (ARO1)
  7. Chorismate synthase (ARO2)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei114NADUniRule annotation1
Binding sitei125Substrate 1UniRule annotation1
Binding sitei141Substrate 2UniRule annotation1
Binding sitei147Substrate 2UniRule annotation1
Binding sitei156NADUniRule annotation1
Binding sitei157Substrate 2UniRule annotation1
Binding sitei185NADUniRule annotation1
Metal bindingi189Zinc; catalyticUniRule annotation1
Binding sitei258Substrate 2UniRule annotation1
Active sitei268Proton acceptor; for 3-dehydroquinate synthase activityUniRule annotation1
Metal bindingi279Zinc; catalyticUniRule annotation1
Binding sitei279Substrate 2UniRule annotation1
Active sitei283Proton acceptor; for 3-dehydroquinate synthase activityUniRule annotation1
Metal bindingi295Zinc; catalyticUniRule annotation1
Binding sitei295Substrate 2UniRule annotation1
Binding sitei364Substrate 2UniRule annotation1
Active sitei853For EPSP synthase activityUniRule annotation1
Active sitei1198Proton acceptor; for 3-dehydroquinate dehydratase activityUniRule annotation1
Active sitei1227Schiff-base intermediate with substrate; for 3-dehydroquinate dehydratase activityUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi43 – 45NADUniRule annotation3
Nucleotide bindingi78 – 81NADUniRule annotation4
Nucleotide bindingi109 – 111NADUniRule annotation3
Nucleotide bindingi134 – 135NADUniRule annotation2
Nucleotide bindingi174 – 177NADUniRule annotation4
Nucleotide bindingi895 – 902ATPUniRule annotation8

GO - Molecular functioni

  • 3-dehydroquinate dehydratase activity Source: SGD
  • 3-dehydroquinate synthase activity Source: SGD
  • 3-phosphoshikimate 1-carboxyvinyltransferase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • shikimate 3-dehydrogenase (NADP+) activity Source: SGD
  • shikimate kinase activity Source: SGD

GO - Biological processi

  • aromatic amino acid family biosynthetic process Source: UniProtKB-KW
  • chorismate biosynthetic process Source: SGD

Keywordsi

Molecular functionKinase, Lyase, Multifunctional enzyme, Oxidoreductase, Transferase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandATP-binding, Metal-binding, NADP, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YDR127W-MONOMER
YEAST:YDR127W-MONOMER
UniPathwayiUPA00053; UER00085
UPA00053; UER00086
UPA00053; UER00087
UPA00053; UER00088
UPA00053; UER00089

Names & Taxonomyi

Protein namesi
Recommended name:
Pentafunctional AROM polypeptideUniRule annotation
Including the following 5 domains:
3-dehydroquinate synthaseUniRule annotation (EC:4.2.3.4UniRule annotation)
Short name:
DHQSUniRule annotation
3-phosphoshikimate 1-carboxyvinyltransferaseUniRule annotation (EC:2.5.1.19UniRule annotation)
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthaseUniRule annotation
Short name:
EPSP synthaseUniRule annotation
Short name:
EPSPSUniRule annotation
Shikimate kinaseUniRule annotation (EC:2.7.1.71UniRule annotation)
Short name:
SKUniRule annotation
3-dehydroquinate dehydrataseUniRule annotation (EC:4.2.1.10UniRule annotation)
Short name:
3-dehydroquinaseUniRule annotation
Shikimate dehydrogenaseUniRule annotation (EC:1.1.1.25UniRule annotation)
Gene namesi
Name:ARO1UniRule annotation
Synonyms:AROM
Ordered Locus Names:YDR127W
ORF Names:YD9302.02
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR127W
SGDiS000002534 ARO1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001408621 – 1588Pentafunctional AROM polypeptideAdd BLAST1588

Proteomic databases

MaxQBiP08566
PaxDbiP08566
PRIDEiP08566

PTM databases

iPTMnetiP08566

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32183615 interactors.
DIPiDIP-2611N
IntActiP08566 71 interactors.
MINTiP08566
STRINGi4932.YDR127W

Structurei

3D structure databases

ProteinModelPortaliP08566
SMRiP08566
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 3923-dehydroquinate synthaseAdd BLAST392
Regioni189 – 192Substrate binding 2UniRule annotation4
Regioni272 – 276Substrate binding 2UniRule annotation5
Regioni405 – 871EPSP synthaseAdd BLAST467
Regioni890 – 1080Shikimate kinaseAdd BLAST191
Regioni1081 – 12933-dehydroquinaseAdd BLAST213
Regioni1306 – 1588Shikimate dehydrogenaseAdd BLAST283

Sequence similaritiesi

In the N-terminal section; belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family.UniRule annotation
In the 2nd section; belongs to the EPSP synthase family.UniRule annotation
In the 3rd section; belongs to the shikimate kinase family.UniRule annotation
In the 4th section; belongs to the type-I 3-dehydroquinase family.UniRule annotation
In the C-terminal section; belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000011238
HOGENOMiHOG000205493
InParanoidiP08566
KOiK13830
OMAiKVSSQYV
OrthoDBiEOG092C02JU

Family and domain databases

CDDicd00502 DHQase_I, 1 hit
cd01556 EPSP_synthase, 1 hit
cd00464 SK, 1 hit
Gene3Di3.20.20.701 hit
3.65.10.102 hits
HAMAPiMF_00210 EPSP_synth, 1 hit
MF_03143 Pentafunct_AroM, 1 hit
MF_00109 Shikimate_kinase, 1 hit
InterProiView protein in InterPro
IPR018508 3-dehydroquinate_DH_AS
IPR013785 Aldolase_TIM
IPR016037 DHQ_synth_AroB
IPR030960 DHQS/DOIS
IPR001381 DHquinase_I
IPR001986 Enolpyruvate_Tfrase_dom
IPR036968 Enolpyruvate_Tfrase_sf
IPR006264 EPSP_synthase
IPR023193 EPSP_synthase_CS
IPR036291 NAD(P)-bd_dom_sf
IPR027417 P-loop_NTPase
IPR008289 Pentafunct_AroM
IPR013792 RNA3'P_cycl/enolpyr_Trfase_a/b
IPR031322 Shikimate/glucono_kinase
IPR013708 Shikimate_DH-bd_N
IPR010110 Shikimate_DH_AroM-type
IPR000623 Shikimate_kinase/TSH1
IPR023000 Shikimate_kinase_CS
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF01761 DHQ_synthase, 1 hit
PF01487 DHquinase_I, 1 hit
PF00275 EPSP_synthase, 1 hit
PF01488 Shikimate_DH, 1 hit
PF08501 Shikimate_dh_N, 1 hit
PF01202 SKI, 1 hit
PIRSFiPIRSF000514 Pentafunct_AroM, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF52540 SSF52540, 1 hit
SSF55205 SSF55205, 1 hit
TIGRFAMsiTIGR01356 aroA, 1 hit
TIGR01357 aroB, 1 hit
TIGR01093 aroD, 1 hit
TIGR01809 Shik-DH-AROM, 1 hit
PROSITEiView protein in PROSITE
PS01028 DEHYDROQUINASE_I, 1 hit
PS00104 EPSP_SYNTHASE_1, 1 hit
PS00885 EPSP_SYNTHASE_2, 1 hit
PS01128 SHIKIMATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

P08566-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQLAKVPIL GNDIIHVGYN IHDHLVETII KHCPSSTYVI CNDTNLSKVP
60 70 80 90 100
YYQQLVLEFK ASLPEGSRLL TYVVKPGETS KSRETKAQLE DYLLVEGCTR
110 120 130 140 150
DTVMVAIGGG VIGDMIGFVA STFMRGVRVV QVPTSLLAMV DSSIGGKTAI
160 170 180 190 200
DTPLGKNFIG AFWQPKFVLV DIKWLETLAK REFINGMAEV IKTACIWNAD
210 220 230 240 250
EFTRLESNAS LFLNVVNGAK NVKVTNQLTN EIDEISNTDI EAMLDHTYKL
260 270 280 290 300
VLESIKVKAE VVSSDERESS LRNLLNFGHS IGHAYEAILT PQALHGECVS
310 320 330 340 350
IGMVKEAELS RYFGILSPTQ VARLSKILVA YGLPVSPDEK WFKELTLHKK
360 370 380 390 400
TPLDILLKKM SIDKKNEGSK KKVVILESIG KCYGDSAQFV SDEDLRFILT
410 420 430 440 450
DETLVYPFKD IPADQQKVVI PPGSKSISNR ALILAALGEG QCKIKNLLHS
460 470 480 490 500
DDTKHMLTAV HELKGATISW EDNGETVVVE GHGGSTLSAC ADPLYLGNAG
510 520 530 540 550
TASRFLTSLA ALVNSTSSQK YIVLTGNARM QQRPIAPLVD SLRANGTKIE
560 570 580 590 600
YLNNEGSLPI KVYTDSVFKG GRIELAATVS SQYVSSILMC APYAEEPVTL
610 620 630 640 650
ALVGGKPISK LYVDMTIKMM EKFGINVETS TTEPYTYYIP KGHYINPSEY
660 670 680 690 700
VIESDASSAT YPLAFAAMTG TTVTVPNIGF ESLQGDARFA RDVLKPMGCK
710 720 730 740 750
ITQTATSTTV SGPPVGTLKP LKHVDMEPMT DAFLTACVVA AISHDSDPNS
760 770 780 790 800
ANTTTIEGIA NQRVKECNRI LAMATELAKF GVKTTELPDG IQVHGLNSIK
810 820 830 840 850
DLKVPSDSSG PVGVCTYDDH RVAMSFSLLA GMVNSQNERD EVANPVRILE
860 870 880 890 900
RHCTGKTWPG WWDVLHSELG AKLDGAEPLE CTSKKNSKKS VVIIGMRAAG
910 920 930 940 950
KTTISKWCAS ALGYKLVDLD ELFEQQHNNQ SVKQFVVENG WEKFREEETR
960 970 980 990 1000
IFKEVIQNYG DDGYVFSTGG GIVESAESRK ALKDFASSGG YVLHLHRDIE
1010 1020 1030 1040 1050
ETIVFLQSDP SRPAYVEEIR EVWNRREGWY KECSNFSFFA PHCSAEAEFQ
1060 1070 1080 1090 1100
ALRRSFSKYI ATITGVREIE IPSGRSAFVC LTFDDLTEQT ENLTPICYGC
1110 1120 1130 1140 1150
EAVEVRVDHL ANYSADFVSK QLSILRKATD SIPIIFTVRT MKQGGNFPDE
1160 1170 1180 1190 1200
EFKTLRELYD IALKNGVEFL DLELTLPTDI QYEVINKRGN TKIIGSHHDF
1210 1220 1230 1240 1250
QGLYSWDDAE WENRFNQALT LDVDVVKFVG TAVNFEDNLR LEHFRDTHKN
1260 1270 1280 1290 1300
KPLIAVNMTS KGSISRVLNN VLTPVTSDLL PNSAAPGQLT VAQINKMYTS
1310 1320 1330 1340 1350
MGGIEPKELF VVGKPIGHSR SPILHNTGYE ILGLPHKFDK FETESAQLVK
1360 1370 1380 1390 1400
EKLLDGNKNF GGAAVTIPLK LDIMQYMDEL TDAAKVIGAV NTVIPLGNKK
1410 1420 1430 1440 1450
FKGDNTDWLG IRNALINNGV PEYVGHTAGL VIGAGGTSRA ALYALHSLGC
1460 1470 1480 1490 1500
KKIFIINRTT SKLKPLIESL PSEFNIIGIE STKSIEEIKE HVGVAVSCVP
1510 1520 1530 1540 1550
ADKPLDDELL SKLERFLVKG AHAAFVPTLL EAAYKPSVTP VMTISQDKYQ
1560 1570 1580
WHVVPGSQML VHQGVAQFEK WTGFKGPFKA IFDAVTKE
Length:1,588
Mass (Da):174,755
Last modified:August 1, 1988 - v1
Checksum:i9880EE2423CDE410
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06077 Genomic DNA Translation: CAA29458.1
Z48179 Genomic DNA Translation: CAA88208.1
X13802 Genomic DNA Translation: CAA32036.1
X13803 Genomic DNA Translation: CAA32037.1
BK006938 Genomic DNA Translation: DAA11973.1
PIRiA32519 BVBYA1
RefSeqiNP_010412.1, NM_001180435.1

Genome annotation databases

EnsemblFungiiYDR127W; YDR127W; YDR127W
GeneIDi851705
KEGGisce:YDR127W

Similar proteinsi

Entry informationi

Entry nameiARO1_YEAST
AccessioniPrimary (citable) accession number: P08566
Secondary accession number(s): D6VSB3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: March 28, 2018
This is version 189 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome