Reviewed, UniProtKB/Swiss-Prot P08566 (ARO1_YEAST)

Names and origin

Protein names

Recommended name:
    Pentafunctional AROM polypeptide
Including the following 5 domains:
    1- Recommended name:
            3-dehydroquinate synthase
              EC=4.2.3.4
    2- Recommended name:
            3-phosphoshikimate 1-carboxyvinyltransferase
              EC=2.5.1.19
        Alternative name(s):
            5-enolpyruvylshikimate-3-phosphate synthase
              Short name=EPSP synthase
              Short name=EPSPS
    3- Recommended name:
            Shikimate kinase
              EC=2.7.1.71
    4- Recommended name:
            3-dehydroquinate dehydratase
                Short name=3-dehydroquinase
              EC=4.2.1.10
    5- Recommended name:
            Shikimate dehydrogenase
              EC=1.1.1.25

Gene names

Name:	ARO1
Synonyms:	AROM
Ordered Locus Names:	YDR127W
ORF Names:	YD9302.02

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1588 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The AROM polypeptide catalyzes 5 consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis.
Catalytic activity	3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate = 3-dehydroquinate + phosphate. 3-dehydroquinate = 3-dehydroshikimate + H2O. Shikimate + NADP+ = 3-dehydroshikimate + NADPH. ATP + shikimate = ADP + shikimate 3-phosphate. Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7. Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.
Subcellular location	Cytoplasm.
Miscellaneous	Present with 6420 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	In the N-terminal section; belongs to the dehydroquinate synthase family. In the 2nd section; belongs to the EPSP synthase family. In the 3rd section; belongs to the shikimate kinase family. In the C-terminal section; belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Kinase Lyase Oxidoreductase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	3-dehydroquinate dehydratase activity Inferred from direct assay. Source: SGD 3-dehydroquinate synthase activity Inferred from direct assay. Source: SGD 3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from direct assay. Source: SGD ATP binding Inferred from electronic annotation. Source: UniProtKB-KW shikimate 5-dehydrogenase activity Inferred from direct assay. Source: SGD shikimate kinase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1588	1588	Pentafunctional AROM polypeptide		PRO_0000140862
Regions
	Nucleotide binding	895 – 902	8	ATP By similarity
	Region	1 – 392	392	3-dehydroquinate synthase
	Region	404 – 866	463	EPSP synthase
	Region	887 – 1060	174	Shikimate kinase
	Region	1061 – 1293	233	3-dehydroquinase
	Region	1306 – 1588	283	Shikimate dehydrogenase
Sites
	Active site	853	1	Potential
	Active site	1198	1	Proton acceptor By similarity
	Active site	1227	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08566-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: 9880EE2423CDE410	FASTA	1,588	174,755
	10 20 30 40 50 60 MVQLAKVPIL GNDIIHVGYN IHDHLVETII KHCPSSTYVI CNDTNLSKVP YYQQLVLEFK 70 80 90 100 110 120 ASLPEGSRLL TYVVKPGETS KSRETKAQLE DYLLVEGCTR DTVMVAIGGG VIGDMIGFVA 130 140 150 160 170 180 STFMRGVRVV QVPTSLLAMV DSSIGGKTAI DTPLGKNFIG AFWQPKFVLV DIKWLETLAK 190 200 210 220 230 240 REFINGMAEV IKTACIWNAD EFTRLESNAS LFLNVVNGAK NVKVTNQLTN EIDEISNTDI 250 260 270 280 290 300 EAMLDHTYKL VLESIKVKAE VVSSDERESS LRNLLNFGHS IGHAYEAILT PQALHGECVS 310 320 330 340 350 360 IGMVKEAELS RYFGILSPTQ VARLSKILVA YGLPVSPDEK WFKELTLHKK TPLDILLKKM 370 380 390 400 410 420 SIDKKNEGSK KKVVILESIG KCYGDSAQFV SDEDLRFILT DETLVYPFKD IPADQQKVVI 430 440 450 460 470 480 PPGSKSISNR ALILAALGEG QCKIKNLLHS DDTKHMLTAV HELKGATISW EDNGETVVVE 490 500 510 520 530 540 GHGGSTLSAC ADPLYLGNAG TASRFLTSLA ALVNSTSSQK YIVLTGNARM QQRPIAPLVD 550 560 570 580 590 600 SLRANGTKIE YLNNEGSLPI KVYTDSVFKG GRIELAATVS SQYVSSILMC APYAEEPVTL 610 620 630 640 650 660 ALVGGKPISK LYVDMTIKMM EKFGINVETS TTEPYTYYIP KGHYINPSEY VIESDASSAT 670 680 690 700 710 720 YPLAFAAMTG TTVTVPNIGF ESLQGDARFA RDVLKPMGCK ITQTATSTTV SGPPVGTLKP 730 740 750 760 770 780 LKHVDMEPMT DAFLTACVVA AISHDSDPNS ANTTTIEGIA NQRVKECNRI LAMATELAKF 790 800 810 820 830 840 GVKTTELPDG IQVHGLNSIK DLKVPSDSSG PVGVCTYDDH RVAMSFSLLA GMVNSQNERD 850 860 870 880 890 900 EVANPVRILE RHCTGKTWPG WWDVLHSELG AKLDGAEPLE CTSKKNSKKS VVIIGMRAAG 910 920 930 940 950 960 KTTISKWCAS ALGYKLVDLD ELFEQQHNNQ SVKQFVVENG WEKFREEETR IFKEVIQNYG 970 980 990 1000 1010 1020 DDGYVFSTGG GIVESAESRK ALKDFASSGG YVLHLHRDIE ETIVFLQSDP SRPAYVEEIR 1030 1040 1050 1060 1070 1080 EVWNRREGWY KECSNFSFFA PHCSAEAEFQ ALRRSFSKYI ATITGVREIE IPSGRSAFVC 1090 1100 1110 1120 1130 1140 LTFDDLTEQT ENLTPICYGC EAVEVRVDHL ANYSADFVSK QLSILRKATD SIPIIFTVRT 1150 1160 1170 1180 1190 1200 MKQGGNFPDE EFKTLRELYD IALKNGVEFL DLELTLPTDI QYEVINKRGN TKIIGSHHDF 1210 1220 1230 1240 1250 1260 QGLYSWDDAE WENRFNQALT LDVDVVKFVG TAVNFEDNLR LEHFRDTHKN KPLIAVNMTS 1270 1280 1290 1300 1310 1320 KGSISRVLNN VLTPVTSDLL PNSAAPGQLT VAQINKMYTS MGGIEPKELF VVGKPIGHSR 1330 1340 1350 1360 1370 1380 SPILHNTGYE ILGLPHKFDK FETESAQLVK EKLLDGNKNF GGAAVTIPLK LDIMQYMDEL 1390 1400 1410 1420 1430 1440 TDAAKVIGAV NTVIPLGNKK FKGDNTDWLG IRNALINNGV PEYVGHTAGL VIGAGGTSRA 1450 1460 1470 1480 1490 1500 ALYALHSLGC KKIFIINRTT SKLKPLIESL PSEFNIIGIE STKSIEEIKE HVGVAVSCVP 1510 1520 1530 1540 1550 1560 ADKPLDDELL SKLERFLVKG AHAAFVPTLL EAAYKPSVTP VMTISQDKYQ WHVVPGSQML 1570 1580 VHQGVAQFEK WTGFKGPFKA IFDAVTKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The pentafunctional arom enzyme of Saccharomyces cerevisiae is a mosaic of monofunctional domains." Duncan K., Edwards R.M., Coggins J.R. Biochem. J. 246:375-386(1987) [PubMed: 2825635] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"The Saccharomyces cerevisiae ARO1 gene. An example of the co-ordinate regulation of five enzymes on a single biosynthetic pathway." Duncan K., Edwards R.M., Coggins J.R. FEBS Lett. 241:83-88(1988) [PubMed: 2848727] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-44 AND 1557-1588.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X06077 Genomic DNA. Translation: CAA29458.1. Z48179 Genomic DNA. Translation: CAA88208.1. X13802 Genomic DNA. Translation: CAA32036.1. X13803 Genomic DNA. Translation: CAA32037.1.
PIR	BVBYA1. A32519.
RefSeq	NP_010412.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2611N.
IntAct	P08566. 106 interactions.
STRING	P08566.
Proteomic databases
PeptideAtlas	P08566.
PRIDE	P08566.
Genome annotation databases
Ensembl	YDR127W; YDR127W; YDR127W; Saccharomyces cerevisiae. [Genome view]
GeneID	851705.
KEGG	sce:YDR127W.
NMPDR	fig|4932.3.peg.1161.
Organism-specific databases
CYGD	YDR127w.
SGD	S000002534. ARO1.
Phylogenomic databases
eggNOG	fuNOG05766.
HOGENOM	HBG398536.
OMA	SSQYVSS.
OrthoDB	EOG91G4MC.
PhylomeDB	P08566.
Enzyme and pathway databases
BioCyc	MetaCyc:YDR127W-MONOMER.
BRENDA	1.1.1.25. 250. 2.5.1.19. 250. 2.7.1.71. 250. 4.2.1.10. 250. 4.2.3.4. 250.
Gene expression databases
ArrayExpress	P08566.
Genevestigator	P08566.
GermOnline	YDR127W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018508. 3-dehydroquinate_DH_AS. IPR013785. Aldolase_TIM. IPR002658. DHQ_synth_AroB. IPR016037. DHQ_synth_AroB_sub. IPR001381. DHquinase_I. IPR001986. EPSP_synthase_core. IPR006264. EPSP_synthase_subgroup. IPR016040. NAD(P)-bd_dom. IPR008289. Pentafunct_AroM. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. IPR010110. Shik_DH_AROM. IPR000623. Shik_kinase. IPR013708. Shikimate_DH-bd_N. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. G3DSA:3.65.10.10. EPSP_synthase. 1 hit.
PANTHER	PTHR21090:SF1. DHQ_synth_AroB. 1 hit.
Pfam	PF01761. DHQ_synthase. 1 hit. PF01487. DHquinase_I. 1 hit. PF00275. EPSP_synthase. 1 hit. PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. PF01202. SKI. 1 hit. [Graphical view]
PIRSF	PIRSF000514. Pentafunct_AroM. 1 hit.
PRINTS	PR01100. SHIKIMTKNASE.
TIGRFAMs	TIGR01356. aroA. 1 hit. TIGR01357. aroB. 1 hit. TIGR01093. aroD. 1 hit. TIGR01809. Shik-DH-AROM. 1 hit.
PROSITE	PS01028. DEHYDROQUINASE_I. 1 hit. PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. PS01128. SHIKIMATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	969386.

Entry information

Entry name

ARO1_YEAST

Accession

Primary (citable) accession number: P08566

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	February 9, 2010
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)