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Protein

Baseplate hub protein gp44

Gene

P

Organism
Enterobacteria phage Mu (Bacteriophage Mu)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Forms the central cylindrical hub of the baseplate and plays an important role in baseplate and tail assembly (Probable). Core component of the initiator complex that triggers the tail tube polymerization during tail assembly (Probable). Forms a conduit that probably functions as an extension of the tail tube allowing viral DNA release during ejection. Might facilitate the interaction of the virus with the host cell surface through electrostatic interactions during virus entry into host cell.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi209 – 228H-T-H motifSequence analysisAdd BLAST20

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Viral contractile tail ejection system

Keywords - Biological processi

Viral genome ejection through host cell envelope, Viral penetration into host cytoplasm, Viral tail assembly, Virus entry into host cell, Virus exit from host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Baseplate hub protein gp44
Alternative name(s):
43 kDa tail protein
Gene product 44
Short name:
gp44
Gene product P
Short name:
gpP
Gene namesi
Name:P
Ordered Locus Names:Mup44
OrganismiEnterobacteria phage Mu (Bacteriophage Mu)
Taxonomic identifieri10677 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeMulikevirus
Virus hostiEnterobacteriaceae [TaxID: 543]
Proteomesi
  • UP000002611 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Viral baseplate protein, Viral tail protein, Virion

Pathology & Biotechi

Disruption phenotypei

No tail is synthesized.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000776921 – 379Baseplate hub protein gp44Add BLAST379

Post-translational modificationi

Cleavage of the C-terminus gives rise to a shorter 40 kDa form.

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle. Expression of late genes is activated by the viral late transcription activator C.1 Publication

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Heterotrimer of one uncleaved (42 kDa) and two cleaved (40 kDa) forms. Forms a hub-like structure with an inner diameter of 25 Angstroms through which DNA can presumably pass during infection. Part of a complex composed of three DNA circularization protein N, three baseplate hub protein gp44 and six wedge sub-complex (made of baseplate proteins gp46, gp47, gp48).2 Publications

Structurei

Secondary structure

1379
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 9Combined sources5
Beta strandi12 – 15Combined sources4
Beta strandi18 – 25Combined sources8
Beta strandi32 – 39Combined sources8
Helixi46 – 48Combined sources3
Beta strandi53 – 58Combined sources6
Beta strandi61 – 75Combined sources15
Beta strandi80 – 88Combined sources9
Helixi91 – 95Combined sources5
Beta strandi101 – 108Combined sources8
Helixi110 – 118Combined sources9
Helixi119 – 121Combined sources3
Beta strandi125 – 127Combined sources3
Helixi132 – 135Combined sources4
Beta strandi138 – 142Combined sources5
Helixi149 – 158Combined sources10
Turni159 – 161Combined sources3
Beta strandi163 – 166Combined sources4
Helixi168 – 170Combined sources3
Beta strandi172 – 175Combined sources4
Beta strandi180 – 187Combined sources8
Turni188 – 190Combined sources3
Beta strandi191 – 198Combined sources8
Beta strandi205 – 209Combined sources5
Beta strandi233 – 236Combined sources4
Beta strandi245 – 248Combined sources4
Helixi256 – 274Combined sources19
Beta strandi275 – 285Combined sources11
Beta strandi297 – 302Combined sources6
Helixi303 – 305Combined sources3
Beta strandi312 – 322Combined sources11
Turni323 – 325Combined sources3
Beta strandi326 – 335Combined sources10
Helixi336 – 339Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WRUX-ray2.10A1-379[»]
ProteinModelPortaliP08558.
SMRiP08558.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08558.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni216 – 224DisorderedSequence analysis9
Regioni345 – 379DisorderedSequence analysisAdd BLAST35

Domaini

C-terminal region forms a flexible domain.Curated

Family and domain databases

Gene3Di3.30.1920.10. 1 hit.
InterProiIPR023399. Baseplate-like_2-layer_sand.
IPR026276. Baseplate_GpP.
[Graphical view]
PIRSFiPIRSF004440. GpP. 1 hit.

Sequencei

Sequence statusi: Complete.

P08558-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNTVTLRAD GRLFTGWTSV SVTRSIESVA GYFELGVNVP PGTDLSGLAP
60 70 80 90 100
GKKFTLEIGG QIVCTGYIDS RRRQMTADSM KITVAGRDKT ADLIDCAAVY
110 120 130 140 150
SGGQWKNRTL EQIARDLCAP YGVTVRWELS DKESSAAFPG FTLDHSETVY
160 170 180 190 200
EALVRASRAR GVLMTSNAAG ELVFSRAAST ATDELVLGEN LLTLDFEEDF
210 220 230 240 250
RDRFSEYTVK GYARANGAEG DDIDAKSIVS RKGTATDSDV TRYRPMIIIA
260 270 280 290 300
DSKITAKDAQ ARALREQRRR LAKSITFEAE IDGWTRKDGQ LWMPNLLVTI
310 320 330 340 350
DASKYAIKTT ELLVSKVTLI LNDQDGLKTR VSLAPREGFL VPVESDRKNR
360 370
KGGDSNGGID ALVEDYYRRH PEKTPPWKE
Length:379
Mass (Da):41,785
Last modified:August 1, 1988 - v1
Checksum:iE09AED2158D13CA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06796 Genomic DNA. Translation: CAA29956.1.
AF083977 Genomic DNA. Translation: AAF01122.1.
PIRiS01891. ZPBPMU.
RefSeqiNP_050648.1. NC_000929.1.

Genome annotation databases

GeneIDi2636285.
KEGGivg:2636285.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06796 Genomic DNA. Translation: CAA29956.1.
AF083977 Genomic DNA. Translation: AAF01122.1.
PIRiS01891. ZPBPMU.
RefSeqiNP_050648.1. NC_000929.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WRUX-ray2.10A1-379[»]
ProteinModelPortaliP08558.
SMRiP08558.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2636285.
KEGGivg:2636285.

Miscellaneous databases

EvolutionaryTraceiP08558.

Family and domain databases

Gene3Di3.30.1920.10. 1 hit.
InterProiIPR023399. Baseplate-like_2-layer_sand.
IPR026276. Baseplate_GpP.
[Graphical view]
PIRSFiPIRSF004440. GpP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBP44_BPMU
AccessioniPrimary (citable) accession number: P08558
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 2, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.