Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Baseplate hub protein gp44

Gene

P

Organism
Enterobacteria phage Mu (Bacteriophage Mu)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Forms the central cylindrical hub of the baseplate and plays an important role in baseplate and tail assembly (Probable). Core component of the initiator complex that triggers the tail tube polymerization during tail assembly (Probable). Forms a conduit that probably functions as an extension of the tail tube allowing viral DNA release during ejection. Might facilitate the interaction of the virus with the host cell surface through electrostatic interactions during virus entry into host cell.Curated

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi209 – 22820H-T-H motifSequence analysisAdd
BLAST

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Viral contractile tail ejection system

Keywords - Biological processi

Viral genome ejection through host cell envelope, Viral penetration into host cytoplasm, Viral tail assembly, Virus entry into host cell, Virus exit from host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Baseplate hub protein gp44
Alternative name(s):
43 kDa tail protein
Gene product 44
Short name:
gp44
Gene product P
Short name:
gpP
Gene namesi
Name:P
Ordered Locus Names:Mup44
OrganismiEnterobacteria phage Mu (Bacteriophage Mu)
Taxonomic identifieri10677 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeMulikevirus
Virus hostiEnterobacteriaceae [TaxID: 543]
Proteomesi
  • UP000002611 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Viral baseplate protein, Viral tail protein, Virion

Pathology & Biotechi

Disruption phenotypei

No tail is synthesized.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 379379Baseplate hub protein gp44PRO_0000077692Add
BLAST

Post-translational modificationi

Cleavage of the C-terminus gives rise to a shorter 40 kDa form.

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle. Expression of late genes is activated by the viral late transcription activator C.1 Publication

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Heterotrimer of one uncleaved (42 kDa) and two cleaved (40 kDa) forms. Forms a hub-like structure with an inner diameter of 25 Angstroms through which DNA can presumably pass during infection. Part of a complex composed of three DNA circularization protein N, three baseplate hub protein gp44 and six wedge sub-complex (made of baseplate proteins gp46, gp47, gp48).2 Publications

Structurei

Secondary structure

1
379
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 95Combined sources
Beta strandi12 – 154Combined sources
Beta strandi18 – 258Combined sources
Beta strandi32 – 398Combined sources
Helixi46 – 483Combined sources
Beta strandi53 – 586Combined sources
Beta strandi61 – 7515Combined sources
Beta strandi80 – 889Combined sources
Helixi91 – 955Combined sources
Beta strandi101 – 1088Combined sources
Helixi110 – 1189Combined sources
Helixi119 – 1213Combined sources
Beta strandi125 – 1273Combined sources
Helixi132 – 1354Combined sources
Beta strandi138 – 1425Combined sources
Helixi149 – 15810Combined sources
Turni159 – 1613Combined sources
Beta strandi163 – 1664Combined sources
Helixi168 – 1703Combined sources
Beta strandi172 – 1754Combined sources
Beta strandi180 – 1878Combined sources
Turni188 – 1903Combined sources
Beta strandi191 – 1988Combined sources
Beta strandi205 – 2095Combined sources
Beta strandi233 – 2364Combined sources
Beta strandi245 – 2484Combined sources
Helixi256 – 27419Combined sources
Beta strandi275 – 28511Combined sources
Beta strandi297 – 3026Combined sources
Helixi303 – 3053Combined sources
Beta strandi312 – 32211Combined sources
Turni323 – 3253Combined sources
Beta strandi326 – 33510Combined sources
Helixi336 – 3394Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WRUX-ray2.10A1-379[»]
ProteinModelPortaliP08558.
SMRiP08558. Positions 3-346.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08558.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni216 – 2249DisorderedSequence analysis
Regioni345 – 37935DisorderedSequence analysisAdd
BLAST

Domaini

C-terminal region forms a flexible domain.Curated

Family and domain databases

Gene3Di3.30.1920.10. 1 hit.
InterProiIPR023399. Baseplate-like_2-layer_sand.
IPR026276. Baseplate_GpP.
[Graphical view]
PIRSFiPIRSF004440. GpP. 1 hit.

Sequencei

Sequence statusi: Complete.

P08558-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNTVTLRAD GRLFTGWTSV SVTRSIESVA GYFELGVNVP PGTDLSGLAP
60 70 80 90 100
GKKFTLEIGG QIVCTGYIDS RRRQMTADSM KITVAGRDKT ADLIDCAAVY
110 120 130 140 150
SGGQWKNRTL EQIARDLCAP YGVTVRWELS DKESSAAFPG FTLDHSETVY
160 170 180 190 200
EALVRASRAR GVLMTSNAAG ELVFSRAAST ATDELVLGEN LLTLDFEEDF
210 220 230 240 250
RDRFSEYTVK GYARANGAEG DDIDAKSIVS RKGTATDSDV TRYRPMIIIA
260 270 280 290 300
DSKITAKDAQ ARALREQRRR LAKSITFEAE IDGWTRKDGQ LWMPNLLVTI
310 320 330 340 350
DASKYAIKTT ELLVSKVTLI LNDQDGLKTR VSLAPREGFL VPVESDRKNR
360 370
KGGDSNGGID ALVEDYYRRH PEKTPPWKE
Length:379
Mass (Da):41,785
Last modified:August 1, 1988 - v1
Checksum:iE09AED2158D13CA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06796 Genomic DNA. Translation: CAA29956.1.
AF083977 Genomic DNA. Translation: AAF01122.1.
PIRiS01891. ZPBPMU.
RefSeqiNP_050648.1. NC_000929.1.

Genome annotation databases

GeneIDi2636285.
KEGGivg:2636285.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06796 Genomic DNA. Translation: CAA29956.1.
AF083977 Genomic DNA. Translation: AAF01122.1.
PIRiS01891. ZPBPMU.
RefSeqiNP_050648.1. NC_000929.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WRUX-ray2.10A1-379[»]
ProteinModelPortaliP08558.
SMRiP08558. Positions 3-346.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2636285.
KEGGivg:2636285.

Miscellaneous databases

EvolutionaryTraceiP08558.

Family and domain databases

Gene3Di3.30.1920.10. 1 hit.
InterProiIPR023399. Baseplate-like_2-layer_sand.
IPR026276. Baseplate_GpP.
[Graphical view]
PIRSFiPIRSF004440. GpP. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of bacteriophage Mu N and P genes."
    Chaconas G., Gloor G.
    Nucleic Acids Res. 16:5211-5212(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: MUCTS62PAP1.
  2. "Bacteriophage Mu genome sequence: analysis and comparison with Mu-like prophages in Haemophilus, Neisseria and Deinococcus."
    Morgan G.J., Hatfull G.F., Casjens S., Hendrix R.W.
    J. Mol. Biol. 317:337-359(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Morphogenetic structures present in lysates of amber mutants of bacteriophage Mu."
    Grundy F.J., Howe M.M.
    Virology 143:485-504(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  4. "Mutational analysis of a C-dependent late promoter of bacteriophage Mu."
    Chiang L.W., Howe M.M.
    Genetics 135:619-629(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  5. "Expression and characterization of a baseplate protein for bacteriophage Mu, gp44."
    Kitazawa D., Takeda S., Kageyama Y., Tomihara M., Fukada H.
    J. Biochem. 137:601-606(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, SUBUNIT, CLEAVAGE OF C-TERMINUS.
  6. "Contractile tail machines of bacteriophages."
    Leiman P.G., Shneider M.M.
    Adv. Exp. Med. Biol. 726:93-114(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  7. "Structure of the central hub of bacteriophage Mu baseplate determined by X-ray crystallography of gp44."
    Kondou Y., Kitazawa D., Takeda S., Tsuchiya Y., Yamashita E., Mizuguchi M., Kawano K., Tsukihara T.
    J. Mol. Biol. 352:976-985(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS), SUBCELLULAR LOCATION, SUBUNIT.

Entry informationi

Entry nameiBP44_BPMU
AccessioniPrimary (citable) accession number: P08558
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: October 14, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.