Reviewed,
UniProtKB/Swiss-Prot P08556 (RASN_MOUSE)
Last modified
February 9, 2010.
Version 98.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (2) |
 Third-party data |
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Names and origin
| Protein names | Recommended name: GTPase NRas Alternative name(s): Transforming protein N-Ras | ||
| Gene names |
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| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 189 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. |
| Enzyme regulation | Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). |
| Subcellular location | Cell membrane; Lipid-anchor; Cytoplasmic side By similarity. Golgi apparatus membrane; Lipid-anchor By similarity. Note: Shuttles between the plasma membrane and the Golgi apparatus By similarity. |
| Post-translational modification | Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi By similarity. |
| Sequence similarities | Belongs to the small GTPase superfamily. Ras family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Golgi apparatus Membrane |
| Disease | Proto-oncogene |
| Ligand | GTP-binding Nucleotide-binding |
| PTM | Lipoprotein Methylation Palmitate Phosphoprotein Prenylation |
| Gene Ontology (GO) | |
| Biological process | Ras protein signal transduction Traceable author statement. Source: MGI regulation of cell cycleTraceable author statement. Source: MGI |
| Cellular component | Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW anchored to membraneInferred from electronic annotation. Source: UniProtKB-SubCell membrane fractionInferred from direct assay. Source: MGI plasma membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activityTraceable author statement. Source: MGI |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 186 | 186 | GTPase NRas | PRO_0000043010 | |||||
| Propeptide | 187 – 189 | 3 | Removed in mature form By similarity | PRO_0000043011 | |||||
Regions | |||||||||
| Nucleotide binding | 10 – 17 | 8 | GTP | ||||||
| Nucleotide binding | 57 – 61 | 5 | GTP | ||||||
| Nucleotide binding | 116 – 119 | 4 | GTP | ||||||
| Region | 166 – 185 | 20 | Hypervariable region | ||||||
| Motif | 32 – 40 | 9 | Effector region | ||||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 4 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 186 | 1 | Cysteine methyl ester By similarity | ||||||
| Lipidation | 181 | 1 | S-palmitoyl cysteine By similarity | ||||||
| Lipidation | 186 | 1 | S-farnesyl cysteine By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 189 | 1 | M → MCKTL in CAA31958. Ref.2 | ||||||
Sequences
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References
| [1] | "Loss of the normal N-ras allele in a mouse thymic lymphoma induced by a chemical carcinogen." Guerrero I., Villasante A., Corces V., Pellicer A. Proc. Natl. Acad. Sci. U.S.A. 82:7810-7814(1985) [PubMed: 3865197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Mouse N-ras genes: organization of the functional locus and of a truncated cDNA-like pseudogene." Chang H.Y., Guerrero I., Lake R., Pellicer A., D'Eustachio P. Oncogene Res. 1:129-136(1987) [PubMed: 2835730] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M12124  M12123 Genomic DNA. Translation: AAA39839.1. X13664 mRNA. Translation: CAA31958.1. X06909 Genomic DNA. Translation: CAA30010.1. |
| IPI | IPI00775965. |
| PIR | TVMSNS. A23560. |
| UniGene | Mm.400954 |
3D structure databases | |
| SMR | P08556. Positions 1-166. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P08556. 1 interaction. |
| STRING | P08556. |
Proteomic databases | |
| PRIDE | P08556. |
Genome annotation databases | |
| Ensembl | ENSMUST00000029445; ENSMUSP00000029445; ENSMUSG00000027852; Mus musculus. [Genome view] |
Organism-specific databases | |
| MGI | MGI:97376. Nras. |
Phylogenomic databases | |
| eggNOG | maNOG17728. |
| HOVERGEN | P08556. |
| InParanoid | P08556. |
| PhylomeDB | P08556. |
Gene expression databases | |
| ArrayExpress | P08556. |
| Bgee | P08556. |
| CleanEx | MM_NRAS. |
| Genevestigator | P08556. |
| GermOnline | ENSMUSG00000027852. Mus musculus. |
Family and domain databases | |
| InterPro | IPR003577. GTPase_Ras. IPR013753. Ras. IPR001806. Ras_GTPase. IPR015592. Ras_Ras_related. IPR020849. Ras_small_GTPase. IPR005225. Small_GTP_bd. [Graphical view] |
| PANTHER | PTHR11708:SF125. Ras_Ras_related. 1 hit. |
| Pfam | PF00071. Ras. 1 hit. [Graphical view] |
| PRINTS | PR00449. RASTRNSFRMNG. |
| SMART | SM00173. RAS. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00231. small_GTP. 1 hit. |
| PROSITE | PS51421. RAS. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| SOURCE | Search... |
Entry information
| Entry name | RASN_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P08556 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |
