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Protein

GTPase NRas

Gene

Nras

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 178GTP
Nucleotide bindingi57 – 615GTP
Nucleotide bindingi116 – 1194GTP

GO - Molecular functioni

  • GTPase activity Source: MGI
  • GTP binding Source: UniProtKB-KW
  • protein complex binding Source: MGI

GO - Biological processi

  • defense response to protozoan Source: MGI
  • establishment of protein localization to Golgi Source: MGI
  • negative regulation of skeletal muscle tissue development Source: MGI
  • positive regulation of fibroblast proliferation Source: MGI
  • positive regulation of interferon-gamma production Source: MGI
  • Ras protein signal transduction Source: MGI
  • regulation of cell cycle Source: MGI
  • regulation of gene expression Source: MGI
  • regulation of protein phosphorylation Source: MGI
  • T cell receptor signaling pathway Source: MGI
  • T-helper 1 type immune response Source: MGI
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase NRas
Alternative name(s):
Transforming protein N-Ras
Gene namesi
Name:Nras
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97376. Nras.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • Golgi apparatus Source: MGI
  • Golgi membrane Source: UniProtKB-SubCell
  • membrane Source: MGI
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 186186GTPase NRasPRO_0000043010Add
BLAST
Propeptidei187 – 1893Removed in mature formBy similarityPRO_0000043011

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi181 – 1811S-palmitoyl cysteineBy similarity
Modified residuei186 – 1861Cysteine methyl esterBy similarity
Lipidationi186 – 1861S-farnesyl cysteineBy similarity

Post-translational modificationi

Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi (By similarity).By similarity
Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).By similarity

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Palmitate, Prenylation

Proteomic databases

EPDiP08556.
MaxQBiP08556.
PaxDbiP08556.
PeptideAtlasiP08556.
PRIDEiP08556.

PTM databases

iPTMnetiP08556.
PhosphoSiteiP08556.
SwissPalmiP08556.

Expressioni

Gene expression databases

CleanExiMM_NRAS.

Interactioni

Subunit structurei

Interacts (active GTP-bound form preferentially) with RGS14. Interacts (active GTP-bound form) with RASSF7 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP08556. 1 interaction.
MINTiMINT-1601725.
STRINGi10090.ENSMUSP00000029445.

Structurei

3D structure databases

ProteinModelPortaliP08556.
SMRiP08556. Positions 1-167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni166 – 18520Hypervariable regionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi32 – 409Effector region

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
HOVERGENiHBG009351.
InParanoidiP08556.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08556-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET
60 70 80 90 100
CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNSKSF ADINLYREQI
110 120 130 140 150
KRVKDSDDVP MVLVGNKCDL PTRTVDTKQA HELAKSYGIP FIETSAKTRQ
160 170 180
GVEDAFYTLV REIRQYRLKK LNSSDDGTQG CMGSPCVLM
Length:189
Mass (Da):21,199
Last modified:August 1, 1988 - v1
Checksum:i688D33F6814855B7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti189 – 1891M → MCKTL in CAA31958 (PubMed:2835730).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12124
, M12121, M12122, M12123 Genomic DNA. Translation: AAA39839.1.
X13664 mRNA. Translation: CAA31958.1.
X06909 Genomic DNA. Translation: CAA30010.1.
PIRiA23560. TVMSNS.
UniGeneiMm.400954.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12124
, M12121, M12122, M12123 Genomic DNA. Translation: AAA39839.1.
X13664 mRNA. Translation: CAA31958.1.
X06909 Genomic DNA. Translation: CAA30010.1.
PIRiA23560. TVMSNS.
UniGeneiMm.400954.

3D structure databases

ProteinModelPortaliP08556.
SMRiP08556. Positions 1-167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP08556. 1 interaction.
MINTiMINT-1601725.
STRINGi10090.ENSMUSP00000029445.

PTM databases

iPTMnetiP08556.
PhosphoSiteiP08556.
SwissPalmiP08556.

Proteomic databases

EPDiP08556.
MaxQBiP08556.
PaxDbiP08556.
PeptideAtlasiP08556.
PRIDEiP08556.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:97376. Nras.

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
HOVERGENiHBG009351.
InParanoidiP08556.

Miscellaneous databases

ChiTaRSiNras. mouse.
PROiP08556.
SOURCEiSearch...

Gene expression databases

CleanExiMM_NRAS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRASN_MOUSE
AccessioniPrimary (citable) accession number: P08556
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: July 6, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.