P08553 (NFM_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neurofilament medium polypeptide
Short name=NF-M

Alternative name(s):
160 kDa neurofilament protein
Neurofilament 3
Neurofilament triplet M protein

Gene names

Name:	Nefm
Synonyms:	Nef3, Nfm

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	848 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Post-translational modification	There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization By similarity.
Sequence similarities	Belongs to the intermediate filament family.

Ontologies

Keywords
Cellular component	Intermediate filament
Domain	Coiled coil
PTM	Acetylation Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	axon cargo transport Inferred from mutant phenotype PubMed 10461886. Source: MGI intermediate filament bundle assembly Inferred from genetic interaction PubMed 10461886. Source: MGI microtubule cytoskeleton organization Inferred from genetic interaction PubMed 10461886. Source: MGI neurofilament cytoskeleton organization Inferred from mutant phenotype PubMed 9566972. Source: MGI regulation of axon diameter Inferred from mutant phenotype PubMed 10461886PubMed 9566972. Source: MGI
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara neurofilament Inferred from direct assay PubMed 10221457PubMed 10482234PubMed 12971893PubMed 15248193. Source: MGI neuromuscular junction Inferred from direct assay PubMed 15713258. Source: MGI
Molecular_function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 848	847	Neurofilament medium polypeptide		PRO_0000063795
Regions
	Region	2 – 102	101	Head
	Region	103 – 410	308	Rod
	Region	103 – 134	32	Coil 1A
	Region	135 – 147	13	Linker 1
	Region	148 – 246	99	Coil 1B
	Region	247 – 263	17	Linker 12
	Region	264 – 285	22	Coil 2A
	Region	286 – 289	4	Linker 2
	Region	290 – 410	121	Coil 2B
	Region	411 – 848	438	Tail
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	318	1	Phosphotyrosine Ref.10
	Modified residue	502	1	Phosphoserine Ref.7 Ref.12
	Modified residue	506	1	Phosphoserine Ref.7
	Modified residue	537	1	Phosphoserine By similarity
	Modified residue	545	1	Phosphoserine By similarity
	Modified residue	551	1	Phosphoserine By similarity
	Modified residue	605	1	Phosphoserine Ref.8
	Modified residue	610	1	Phosphoserine Ref.8 Ref.12
	Modified residue	642	1	Phosphothreonine Ref.8
	Modified residue	645	1	Phosphoserine Ref.12
	Modified residue	669	1	Phosphoserine Ref.8
	Modified residue	689	1	Phosphoserine By similarity
	Modified residue	715	1	Phosphoserine Ref.8
	Modified residue	769	1	Phosphoserine Ref.11
	Glycosylation	47	1	O-linked (GlcNAc) Probable
	Glycosylation	430	1	O-linked (GlcNAc) Probable
Experimental info
	Sequence conflict	17	1	V → VP in CAA29127. Ref.1
	Sequence conflict	53	1	K → T in CAA29127. Ref.1
	Sequence conflict	57	1	L → V in CAA29127. Ref.1
	Sequence conflict	234	1	S → R in CAA29127. Ref.1
	Sequence conflict	432	1	S → F in AAA39815. Ref.6
	Sequence conflict	539 – 540	2	QA → RR in AAA39815. Ref.6
	Sequence conflict	628	1	Q → H in ABA46749. Ref.2
	Sequence conflict	628	1	Q → H in BAC34724. Ref.3
	Sequence conflict	628	1	Q → H in BAE37734. Ref.3
	Sequence conflict	696	1	E → K in BAC34724. Ref.3
	Sequence conflict	699	1	P → L in ABA46749. Ref.2
	Sequence conflict	699	1	P → L in BAC34724. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P08553 [UniParc]. Last modified May 1, 2007. Version 4. Checksum: 0783F50558A7D4C3	FASTA	848	95,916
	10 20 30 40 50 60 MSYTLDSLGN PSAYRRVTET RSSFSRVSGS PSSGFRSQSW SRGSPSTVSS SYKRSALAPR 70 80 90 100 110 120 LAYSSAMLSS AESSLDFSQS SSLLNGGSGG DYKLSRSNEK EQLQGLNDRF AGYIEKVHYL 130 140 150 160 170 180 EQQNKEIEAE IQALRQKQAS HAQLGDAYDQ EIRELRATLE MVNHEKAQVQ LDSDHLEEDI 190 200 210 220 230 240 HRLKERFEEE ARLRDDTEAA IRALRKDIEE SSMVKVELDK KVQSLQDEVA FLRSNHEEEV 250 260 270 280 290 300 ADLLAQIQAS HITVERKDYL KTDISTALKE IRSQLECHSD QNMHQAEEWF KCRYAKLTEA 310 320 330 340 350 360 AEQNKEAIRS AKEEIAEYRR QLQSKSIELE SVRGTKESLE RQLSDIEERH NHDLSSYQDT 370 380 390 400 410 420 IQQLENELRG TKWEMARHLR EYQDLLNVKM ALDIEIAAYR KLLEGEETRF STFSGSITGP 430 440 450 460 470 480 LYTHRQPSVT ISSKIQKTKV EAPKLKVQHK FVEEIIEETK VEDEKSEMEE TLTAIAEELA 490 500 510 520 530 540 ASAKEEKEEA EEKEEEPEAE KSPVKSPEAK EEEEEGEKEE EEEGQEEEEE EDEGVKSDQA 550 560 570 580 590 600 EEGGSEKEGS SEKDEGEQEE EEGETEAEGE GEEAEAKEEK KIEGKVEEVA VKEEIKVEKP 610 620 630 640 650 660 EKAKSPMPKS PVEEVKPKPE AKAGKGEQKE EEKVEEEKKE VTKESPKEEK VEKKEEKPKD 670 680 690 700 710 720 VADKKKAESP VKEKAVEEVI TISKSVKVSL EKDTKEEKPQ PQEKVKEKAE EEGGSEEEGS 730 740 750 760 770 780 DRSPQESKKE DIAINGEVEG KEEEEQETQE KGSGREEEKG VVTNGLDVSP AEEKKGEDSS 790 800 810 820 830 840 DDKVVVTKKV EKITSEGGDG ATKYITKSVT VTQKVEEHEE TFEEKLVSTK KVEKVTSHAI VKEVTQGD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and evolutionary origin of the gene encoding mouse NF-M, the middle-molecular-mass neurofilament protein." Levy E., Liem R.K.H., D'Eustachio P., Cowan N.J. Eur. J. Biochem. 166:71-77(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Jensen K.H., Brown A. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Cerebellum.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Eye and Spinal ganglion.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-848.
[5]	Lubec G., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 167-182; 222-233 AND 410-425, MASS SPECTROMETRY. Tissue: Hippocampus.
[6]	"Cloning and developmental expression of the murine neurofilament gene family." Julien J.-P., Meyer D., Flavell D., Hurst J., Grosveld F. Brain Res. 387:243-250(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 322-540.
[7]	"Proteomic analysis of in vivo phosphorylated synaptic proteins." Collins M.O., Yu L., Coba M.P., Husi H., Campuzano I., Blackstock W.P., Choudhary J.S., Grant S.G. J. Biol. Chem. 280:5972-5982(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-502 AND SER-506, MASS SPECTROMETRY. Tissue: Forebrain.
[8]	"Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-605; SER-610; THR-642; SER-669 AND SER-715, MASS SPECTROMETRY. Tissue: Brain.
[9]	"O-linked N-acetylglucosamine proteomics of postsynaptic density preparations using lectin weak affinity chromatography and mass spectrometry." Vosseller K., Trinidad J.C., Chalkley R.J., Specht C.G., Thalhammer A., Lynn A.J., Snedecor J.O., Guan S., Medzihradszky K.F., Maltby D.A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:923-934(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT THR-47 AND THR-430, MASS SPECTROMETRY. Tissue: Brain.
[10]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-318, MASS SPECTROMETRY. Tissue: Brain.
[11]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-769, MASS SPECTROMETRY. Tissue: Brain cortex.
[12]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-502; SER-610 AND SER-645, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X05640 Genomic DNA. Translation: CAA29127.1. DQ201636 mRNA. Translation: ABA46749.1. AK051696 mRNA. Translation: BAC34724.1. AK164318 mRNA. Translation: BAE37734.1. AK165041 mRNA. Translation: BAE38014.1. BC119602 mRNA. Translation: AAI19603.1. BC128564 mRNA. Translation: AAI28565.1. M20481 mRNA. Translation: AAA39815.1.
IPI	IPI00323800.
PIR	B43772. S00030.
RefSeq	NP_032717.2. NM_008691.2.
UniGene	Mm.390700.
3D structure databases
ProteinModelPortal	P08553.
SMR	P08553. Positions 98-247, 263-332, 337-405.
ModBase	Search...
Protein-protein interaction databases
IntAct	P08553. 6 interactions.
PTM databases
PhosphoSite	P08553.
2D gel databases
UCD-2DPAGE	P08553.
Proteomic databases
PaxDb	P08553.
PRIDE	P08553.
Protocols and materials databases
DNASU	18040.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000022638; ENSMUSP00000022638; ENSMUSG00000022054.
GeneID	18040.
KEGG	mmu:18040.
UCSC	uc007ulo.1. mouse.
Organism-specific databases
CTD	4741.
MGI	MGI:97314. Nefm.
Phylogenomic databases
eggNOG	NOG151229.
GeneTree	ENSGT00560000076873.
HOVERGEN	HBG013015.
InParanoid	P08553.
KO	K04573.
OrthoDB	EOG4VMFFD.
Gene expression databases
ArrayExpress	P08553.
Bgee	P08553.
CleanEx	MM_NEFM.
Genevestigator	P08553.
GermOnline	ENSMUSG00000022054. Mus musculus.
Family and domain databases
InterPro	IPR016044. F. IPR001664. IF. IPR006821. Intermed_filament_DNA-bd. IPR018039. Intermediate_filament_CS. IPR002957. Keratin_I. [Graphical view]
PANTHER	PTHR23239. PTHR23239. 1 hit.
Pfam	PF00038. Filament. 1 hit. PF04732. Filament_head. 1 hit. [Graphical view]
PRINTS	PR01248. TYPE1KERATIN.
PROSITE	PS00226. IF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	293149.
SOURCE	Search...

Entry information

Entry name

NFM_MOUSE

Accession

Primary (citable) accession number: P08553
Secondary accession number(s): A2VCT5 , Q0VDM8, Q3HRJ6, Q3TNS4, Q3TPK2, Q61961, Q8BQ20

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	May 1, 2007
Last modified:	May 1, 2013
This is version 134 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program