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Protein

Neurofilament light polypeptide

Gene

Nefl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Miscellaneous

NF-L is the most abundant of the three neurofilament proteins and, like the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments.

GO - Molecular functioni

GO - Biological processi

  • anterograde axonal transport Source: MGI
  • axonal transport of mitochondrion Source: MGI
  • cerebral cortex development Source: Ensembl
  • hippocampus development Source: Ensembl
  • intermediate filament bundle assembly Source: BHF-UCL
  • intermediate filament organization Source: MGI
  • intermediate filament polymerization or depolymerization Source: Ensembl
  • locomotion Source: MGI
  • microtubule cytoskeleton organization Source: MGI
  • negative regulation of neuron apoptotic process Source: MGI
  • neurofilament bundle assembly Source: MGI
  • neurofilament cytoskeleton organization Source: MGI
  • neuromuscular process controlling balance Source: MGI
  • neuron projection morphogenesis Source: MGI
  • peripheral nervous system axon regeneration Source: MGI
  • positive regulation of axonogenesis Source: MGI
  • protein polymerization Source: Ensembl
  • regulation of axon diameter Source: MGI
  • response to acrylamide Source: Ensembl
  • response to corticosterone Source: Ensembl
  • response to peptide hormone Source: Ensembl
  • response to sodium arsenite Source: Ensembl
  • retrograde axonal transport Source: MGI
  • spinal cord development Source: Ensembl

Enzyme and pathway databases

ReactomeiR-MMU-438066 Unblocking of NMDA receptor, glutamate binding and activation
R-MMU-442729 CREB phosphorylation through the activation of CaMKII
R-MMU-442982 Ras activation upon Ca2+ influx through NMDA receptor
R-MMU-5673001 RAF/MAP kinase cascade

Names & Taxonomyi

Protein namesi
Recommended name:
Neurofilament light polypeptide
Short name:
NF-L
Alternative name(s):
68 kDa neurofilament protein
Neurofilament triplet L protein
Gene namesi
Name:Nefl
Synonyms:Nf68, Nfl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:97313 Nefl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Intermediate filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000637882 – 543Neurofilament light polypeptideAdd BLAST542

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Glycosylationi21O-linked (GlcNAc) threonineBy similarity1
Modified residuei23Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei23Omega-N-methylarginine; alternateCombined sources1
Glycosylationi27O-linked (GlcNAc) serineBy similarity1
Modified residuei30Omega-N-methylarginineCombined sources1
Modified residuei43PhosphotyrosineCombined sources1
Modified residuei56Phosphoserine1 Publication1
Modified residuei67PhosphoserineBy similarity1
Modified residuei103PhosphoserineBy similarity1
Modified residuei473PhosphoserineCombined sources1
Modified residuei503PhosphoserineBy similarity1
Modified residuei520PhosphothreonineBy similarity1
Modified residuei523PhosphoserineBy similarity1
Modified residuei532PhosphoserineCombined sources1

Post-translational modificationi

O-glycosylated.1 Publication
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.By similarity
Ubiquitinated in the presence of TRIM2 and UBE2D1.1 Publication

Keywords - PTMi

Acetylation, Glycoprotein, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP08551
MaxQBiP08551
PaxDbiP08551
PeptideAtlasiP08551
PRIDEiP08551

2D gel databases

UCD-2DPAGEiP08551

PTM databases

iPTMnetiP08551
PhosphoSitePlusiP08551
SwissPalmiP08551

Expressioni

Gene expression databases

BgeeiENSMUSG00000022055
CleanExiMM_NEFL
GenevisibleiP08551 MM

Interactioni

Subunit structurei

Interacts with ARHGEF28. Interacts with TRIM2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Trim2Q9ESN62EBI-445199,EBI-8315064

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201757, 19 interactors
DIPiDIP-31944N
IntActiP08551, 15 interactors
MINTiP08551
STRINGi10090.ENSMUSP00000022639

Structurei

3D structure databases

ProteinModelPortaliP08551
SMRiP08551
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini90 – 401IF rodPROSITE-ProRule annotationAdd BLAST312

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 93HeadAdd BLAST92
Regioni94 – 125Coil 1AAdd BLAST32
Regioni126 – 138Linker 1Add BLAST13
Regioni139 – 234Coil 1BAdd BLAST96
Regioni235 – 253Linker 12Add BLAST19
Regioni254 – 272Coil 2AAdd BLAST19
Regioni273 – 281Linker 29
Regioni282 – 397Coil 2BAdd BLAST116
Regioni382 – 392Epitope; recognized by IF-specific monoclonal antibodyAdd BLAST11
Regioni398 – 543TailAdd BLAST146
Regioni398 – 444Tail, subdomain AAdd BLAST47
Regioni445 – 543Tail, subdomain B (acidic)Add BLAST99

Domaini

The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.

Sequence similaritiesi

Belongs to the intermediate filament family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IGME Eukaryota
ENOG410XPTM LUCA
GeneTreeiENSGT00910000143989
HOGENOMiHOG000230977
HOVERGENiHBG013015
InParanoidiP08551
KOiK04572
OMAiEMDVSSK
OrthoDBiEOG091G12MK
PhylomeDBiP08551
TreeFamiTF330122

Family and domain databases

InterProiView protein in InterPro
IPR001664 IF
IPR006821 Intermed_filament_DNA-bd
IPR018039 Intermediate_filament_CS
IPR027692 NF-L
PANTHERiPTHR23239 PTHR23239, 1 hit
PTHR23239:SF22 PTHR23239:SF22, 1 hit
PfamiView protein in Pfam
PF00038 Filament, 1 hit
PF04732 Filament_head, 1 hit
SMARTiView protein in SMART
SM01391 Filament, 1 hit
PROSITEiView protein in PROSITE
PS00226 IF_ROD_1, 1 hit
PS51842 IF_ROD_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08551-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSFGYDPYF STSYKRRYVE TPRVHISSVR SGYSTARSAY SSYSAPVSSS
60 70 80 90 100
LSVRRSYSSS SGSLMPSLEN LDLSQVAAIS NDLKSIRTQE KAQLQDLNDR
110 120 130 140 150
FASFIERVHE LEQQNKVLEA ELLVLRQKHS EPSRFRALYE QEIRDLRLAA
160 170 180 190 200
EDATNEKQAL QGEREGLEET LRNLQARYEE EVLSREDAEG RLMEARKGAD
210 220 230 240 250
EAALARAELE KRIDSLMDEI AFLKKVHEEE IAELQAQIQY AQISVEMDVS
260 270 280 290 300
SKPDLSAALK DIRAQYEKLA AKNMQNAEEW FKSRFTVLTE SAAKNTDAVR
310 320 330 340 350
AAKDEVSESR RLLKAKTLEI EACRGMNEAL EKQLQELEDK QNADISAMQD
360 370 380 390 400
TINKLENELR STKSEMARYL KEYQDLLNVK MALDIEIAAY RKLLEGEETR
410 420 430 440 450
LSFTSVGSIT SGYSQSSQVF GRSAYSGLQS SSYLMSARSF PAYYTSHVQE
460 470 480 490 500
EQTEVEETIE ATKAEEAKDE PPSEGEAEEE EKEKEEGEEE EGAEEEEAAK
510 520 530 540
DESEDTKEEE EGGEGEEEDT KESEEEEKKE ESAGEEQVAK KKD
Length:543
Mass (Da):61,508
Last modified:January 23, 2007 - v5
Checksum:iBC40F8A8A536CFF5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6Y → S in AAA39810 (PubMed:3785173).Curated1
Sequence conflicti9Y → I in AAA39810 (PubMed:3785173).Curated1
Sequence conflicti65M → K in AAA39810 (PubMed:3785173).Curated1
Sequence conflicti73L → V in AAA39810 (PubMed:3785173).Curated1
Sequence conflicti99D → H in AAA39810 (PubMed:3785173).Curated1
Sequence conflicti195A → R in AAA39814 (PubMed:3103856).Curated1
Sequence conflicti203Missing in AAA39814 (PubMed:3103856).Curated1
Sequence conflicti240Y → I in AAA39810 (PubMed:3785173).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20480 mRNA Translation: AAA39814.1
M13016 Genomic DNA Translation: AAA39810.1
DQ201635 mRNA Translation: ABA46748.1
BC029203 mRNA Translation: AAH29203.1
AH002053 Genomic DNA Translation: AAA39812.1
X02165 mRNA Translation: CAB51616.1
CCDSiCCDS27232.1
PIRiA25227 QFMSL
RefSeqiNP_035040.1, NM_010910.1
UniGeneiMm.1956

Genome annotation databases

EnsembliENSMUST00000022639; ENSMUSP00000022639; ENSMUSG00000022055
GeneIDi18039
KEGGimmu:18039
UCSCiuc007uln.1 mouse

Similar proteinsi

Entry informationi

Entry nameiNFL_MOUSE
AccessioniPrimary (citable) accession number: P08551
Secondary accession number(s): Q8K0Z0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 174 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health