Reviewed, UniProtKB/Swiss-Prot P08542 (UD2B3_RAT)

Names and origin

Protein names

Recommended name:
    UDP-glucuronosyltransferase 2B3
      Short name=UDPGT 2B3
    EC=2.4.1.17
Alternative name(s):
    Testosterone, dihydrotestosterone, and beta-estradiol specific
    17-beta-hydroxysteroid specific
    UDPGTr-3
    RLUG38

Gene names

Name:

Ugt2b3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. 2B3 is active on testosterone.
Catalytic activity	UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.
Subcellular location	Microsome membrane; Single-pass membrane protein Potential. Endoplasmic reticulum membrane; Single-pass membrane protein Potential.
Induction	Constitutively expressed.
Sequence similarities	Belongs to the UDP-glycosyltransferase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Signal Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glucuronosyltransferase activity Ref.1 Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	By similarity
	Chain	24 – 530	507	UDP-glucuronosyltransferase 2B3		PRO_0000036027
Regions
	Transmembrane	494 – 510	17	Potential
Experimental info
	Sequence conflict	54	1	T → S in CAA68351. Ref.2
	Sequence conflict	119	1	G → E in CAA68351. Ref.2
	Sequence conflict	376	1	S → G in CAA68351. Ref.2
	Sequence conflict	424	1	S → T in CAA68351. Ref.2
	Sequence conflict	500	1	T → S in CAA68351. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P08542-1 [UniParc]. Last modified February 1, 1991. Version 2. Checksum: BB633D70DBF8A7E4	FASTA	530	60,525
	10 20 30 40 50 60 MPGKWISALL LLQISCCFQS GNCGKVLVWP MEFSHWMNIK TILDELVQRG HEVTVLKPSA 70 80 90 100 110 120 YYVLDPKKSP DLKFETFPTS VSKDELENYF IKLVDVWTYE LQRDTCLSYS PLLQNMIDGF 130 140 150 160 170 180 SDYYLSLCKD TVSNKQLMAK LQESKFDVLL SDPVAACGEL IAEVLHIPFL YSLRFSPGYK 190 200 210 220 230 240 IEKSSGRFIL PPSYVPVILS GMGGPMTFID RVKNMICTLY FDFWFHMFNA KKWDPFYSEI 250 260 270 280 290 300 LGRPTTLAET MGKAEMWLIR SYWDLEFPHP TLPNVDYIGG LQCRPPKPLP KDMEDFVQSS 310 320 330 340 350 360 GEHGVVVFSL GSMVSSMTEE KANAIAWALA QIPQKVLWKF DGKTPATLGP NTRVYKWLPQ 370 380 390 400 410 420 NDLLGHPKTK AFVTHSGANG VYEAIYHGIP MVGIPMFGEQ HDNIAHMVAK GAAVTLNIRT 430 440 450 460 470 480 MSKSDLFNAL KEIINNPFYK KNAVWLSTIH HDQPMKPLDK AVFWIEFVMR HKGAKHLRPL 490 500 510 520 530 GHDLPWYQYH SLDVIGFLLT CSAVIAVLTV KCFLFIYRLF VKKEKKMKNE

References

[1]	"Rat liver UDP-glucuronosyltransferase. Identification of cDNAs encoding two enzymes which glucuronidate testosterone, dihydrotestosterone, and beta-estradiol." McKenzie P.I. J. Biol. Chem. 262:9744-9749(1987) [PubMed: 3110162] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Nucleotide and deduced amino acid sequence of rat liver 17 beta-hydroxysteroid UDP-glucuronosyltransferase." Harding D., Wilson S.M., Jackson M.R., Burchell B., Green M.D., Tephly T.R. Nucleic Acids Res. 15:3936-3936(1987) [PubMed: 3108864] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M31109 mRNA. Translation: AAA41280.1.
Y00156 mRNA. Translation: CAA68351.1.

IPI

IPI00327626.

PIR

S07390.

RefSeq

NP_695226.2.

UniGene

Rn.24945

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HN3	model	-	@	1-530	[»]

SMR

P08542. Positions 287-447.

ModBase

Search...

Protein family/group databases

CAZy

GT1. Glycosyltransferase Family 1.

Proteomic databases

PRIDE

P08542.

Genome annotation databases

Ensembl

ENSRNOG00000033139. Rattus norvegicus. [Contig view]

GeneID

266685.

KEGG

rno:266685.

Organism-specific databases

RGD

628623. Ugt2b3.

Phylogenomic databases

HOVERGEN

P08542.

Enzyme and pathway databases

BRENDA

2.4.1.17. 248.

Gene expression databases

ArrayExpress

P08542.

GermOnline

ENSRNOG00000033139. Rattus norvegicus.

Family and domain databases

InterPro

IPR002213. UDP_glucos_trans.
[Graphical view]

PANTHER

PTHR11926. UDP_glucos_trans. 1 hit.

Pfam

PF00201. UDPGT. 1 hit.
[Graphical view]

PROSITE

PS00375. UDPGT. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

624488.

Entry information

Entry name

UD2B3_RAT

Accession

Primary (citable) accession number: P08542
Secondary accession number(s): P16915

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	February 1, 1991
Last modified:	June 16, 2009
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)