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Protein

UDP-glucuronosyltransferase 2B2

Gene

Ugt2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. 2B2 acts on various endogenous steroids, especially etiocholanolone and androsterone.

Catalytic activityi

UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.

GO - Molecular functioni

  1. glucuronosyltransferase activity Source: RGD

GO - Biological processi

  1. cellular response to hormone stimulus Source: GO_Central
  2. flavonoid biosynthetic process Source: GO_Central
  3. flavonoid glucuronidation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.17. 5301.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucuronosyltransferase 2B2 (EC:2.4.1.17)
Short name:
UDPGT 2B2
Alternative name(s):
3-hydroxyandrogen-specific UDPGT
RLUG23
UDPGTr-4
Gene namesi
Name:Ugt2b
Synonyms:Ugt2b2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3936. Ugt2b.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei494 – 51017HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. intracellular membrane-bounded organelle Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 530507UDP-glucuronosyltransferase 2B2PRO_0000036026Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi316 – 3161N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Autoacylation in vitro.

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP08541.
PRIDEiP08541.

Expressioni

Inductioni

Constitutively expressed.

Gene expression databases

GenevestigatoriP08541.

Interactioni

Protein-protein interaction databases

IntActiP08541. 1 interaction.
MINTiMINT-4564702.
STRINGi10116.ENSRNOP00000034723.

Structurei

3D structure databases

ProteinModelPortaliP08541.
SMRiP08541. Positions 286-447.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1819.
HOGENOMiHOG000220831.
HOVERGENiHBG004033.
InParanoidiP08541.
PhylomeDBiP08541.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08541-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRKWISALF LLQISYCFKS GHCGKVLVWP MDFSHWMNIK IILDELVQRG
60 70 80 90 100
HEVTVLKPSA YFFLDPKKSS DLKFEIFSTS ISKDELQNHF IKLLDVWTYE
110 120 130 140 150
LPRDTCLSYS PILQNLVYEF SYFYLSICKD AVSNKQLMTK LQESKFDVLF
160 170 180 190 200
ADPVASCGDL IAELLHIPFL YSLSFSPGHK LEKSIGKFIL PPSYVPVILS
210 220 230 240 250
GLAGKMTFID RVKNMICMLY FDFWFERLRH KEWDTFYSEI LGRPTTVDET
260 270 280 290 300
MSKVEIWLIR SYWDLKFPHP TLPNVDYIGG LHCKPAKPLP KDMEEFVQSS
310 320 330 340 350
GEHGVVVFSL GSMVSNMTEE KANAIAWALA QIPQKVLWKF DGKTPATLGP
360 370 380 390 400
NTRVYKWLPQ NDLLGHPKTK AFVTHGGANG LYEAIYHGIP MIGIPLFGDQ
410 420 430 440 450
PDNIAHMVAK GAAVSLNIRT MSKLDFLSAL EEVIDNPFYK KNVMLLSTIH
460 470 480 490 500
HDQPMKPLDR AVFWIEFIMR HKGAKHLRPL GHNLPWYQYH SLDVIGFLLT
510 520 530
CFAVIAALTV KCLLFMYRFF VKKEKKMKNE
Length:530
Mass (Da):60,986
Last modified:August 1, 1988 - v1
Checksum:iF2FFF3E23E2D75B2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti159 – 1591D → E in CAA27198 (PubMed:3003696).Curated
Sequence conflicti286 – 2861A → S in CAA27198 (PubMed:3003696).Curated
Sequence conflicti351 – 3511N → I in CAA27198 (PubMed:3003696).Curated
Sequence conflicti363 – 3631L → I in CAA27198 (PubMed:3003696).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02589 Genomic DNA. Translation: AAA42314.1.
M74439 Genomic DNA. No translation available.
X03478 mRNA. Translation: CAA27198.1.
PIRiA40467.
UniGeneiRn.2521.

Genome annotation databases

UCSCiRGD:3936. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02589 Genomic DNA. Translation: AAA42314.1.
M74439 Genomic DNA. No translation available.
X03478 mRNA. Translation: CAA27198.1.
PIRiA40467.
UniGeneiRn.2521.

3D structure databases

ProteinModelPortaliP08541.
SMRiP08541. Positions 286-447.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP08541. 1 interaction.
MINTiMINT-4564702.
STRINGi10116.ENSRNOP00000034723.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Proteomic databases

PaxDbiP08541.
PRIDEiP08541.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:3936. rat.

Organism-specific databases

RGDi3936. Ugt2b.

Phylogenomic databases

eggNOGiCOG1819.
HOGENOMiHOG000220831.
HOVERGENiHBG004033.
InParanoidiP08541.
PhylomeDBiP08541.

Enzyme and pathway databases

BRENDAi2.4.1.17. 5301.

Gene expression databases

GenevestigatoriP08541.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Rat liver UDP-glucuronosyltransferase. cDNA sequence and expression of a form glucuronidating 3-hydroxyandrogens."
    McKenzie P.I.
    J. Biol. Chem. 261:14112-14117(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  2. "Isolation, sequence, and developmental expression of rat UGT2B2: the gene encoding a constitutive UDP glucuronosyltransferase that metabolizes etiocholanolone and androsterone."
    Haque S.J., Peterson D.D., Nebert D.W., McKenzie P.I.
    DNA Cell Biol. 10:515-524(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The full length coding sequence of rat liver androsterone UDP-glucuronyltransferase cDNA and comparison with other members of this gene family."
    Jackson M.R., Burchell B.
    Nucleic Acids Res. 14:779-795(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-530.
    Tissue: Liver.
  4. "Acyl-CoA binding and acylation of UDP-glucuronosyltransferase isoforms of rat liver: their effect on enzyme activity."
    Yamashita A., Watanabe M., Tonegawa T., Sugiura T., Waku K.
    Biochem. J. 312:301-308(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-44, LIPID-BINDING.

Entry informationi

Entry nameiUD2B2_RAT
AccessioniPrimary (citable) accession number: P08541
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: February 4, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.