Reviewed, UniProtKB/Swiss-Prot P08541 (UD2B2_RAT)

Names and origin

Protein names

Recommended name:
    UDP-glucuronosyltransferase 2B2
      Short name=UDPGT 2B2
    EC=2.4.1.17
Alternative name(s):
    3-hydroxyandrogen specific
    UDPGTr-4
    RLUG23

Gene names

Name:	Ugt2b2
Synonyms:	Ugt2b

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. 2B2 acts on various endogenous steroids, especially etiocholanolone and androsterone.
Catalytic activity	UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.
Subcellular location	Microsome membrane; Single-pass membrane protein Potential. Endoplasmic reticulum membrane; Single-pass membrane protein Potential.
Induction	Constitutively expressed.
Post-translational modification	Autoacylation in vitro.
Sequence similarities	Belongs to the UDP-glycosyltransferase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Signal Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glucuronosyltransferase activity Ref.1 Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.4
	Chain	24 – 530	507	UDP-glucuronosyltransferase 2B2		PRO_0000036026
Regions
	Transmembrane	494 – 510	17	Potential
Amino acid modifications
	Glycosylation	316	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	159	1	D → E in CAA27198. Ref.3
	Sequence conflict	286	1	A → S in CAA27198. Ref.3
	Sequence conflict	351	1	N → I in CAA27198. Ref.3
	Sequence conflict	363	1	L → I in CAA27198. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P08541-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: F2FFF3E23E2D75B2	FASTA	530	60,986
	10 20 30 40 50 60 MPRKWISALF LLQISYCFKS GHCGKVLVWP MDFSHWMNIK IILDELVQRG HEVTVLKPSA 70 80 90 100 110 120 YFFLDPKKSS DLKFEIFSTS ISKDELQNHF IKLLDVWTYE LPRDTCLSYS PILQNLVYEF 130 140 150 160 170 180 SYFYLSICKD AVSNKQLMTK LQESKFDVLF ADPVASCGDL IAELLHIPFL YSLSFSPGHK 190 200 210 220 230 240 LEKSIGKFIL PPSYVPVILS GLAGKMTFID RVKNMICMLY FDFWFERLRH KEWDTFYSEI 250 260 270 280 290 300 LGRPTTVDET MSKVEIWLIR SYWDLKFPHP TLPNVDYIGG LHCKPAKPLP KDMEEFVQSS 310 320 330 340 350 360 GEHGVVVFSL GSMVSNMTEE KANAIAWALA QIPQKVLWKF DGKTPATLGP NTRVYKWLPQ 370 380 390 400 410 420 NDLLGHPKTK AFVTHGGANG LYEAIYHGIP MIGIPLFGDQ PDNIAHMVAK GAAVSLNIRT 430 440 450 460 470 480 MSKLDFLSAL EEVIDNPFYK KNVMLLSTIH HDQPMKPLDR AVFWIEFIMR HKGAKHLRPL 490 500 510 520 530 GHNLPWYQYH SLDVIGFLLT CFAVIAALTV KCLLFMYRFF VKKEKKMKNE

References

[1]	"Rat liver UDP-glucuronosyltransferase. cDNA sequence and expression of a form glucuronidating 3-hydroxyandrogens." McKenzie P.I. J. Biol. Chem. 261:14112-14117(1986) [PubMed: 2429951] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Liver.
[2]	"Isolation, sequence, and developmental expression of rat UGT2B2: the gene encoding a constitutive UDP glucuronosyltransferase that metabolizes etiocholanolone and androsterone." Haque S.J., Peterson D.D., Nebert D.W., McKenzie P.I. DNA Cell Biol. 10:515-524(1991) [PubMed: 1909872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The full length coding sequence of rat liver androsterone UDP-glucuronyltransferase cDNA and comparison with other members of this gene family." Jackson M.R., Burchell B. Nucleic Acids Res. 14:779-795(1986) [PubMed: 3003696] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-530. Tissue: Liver.
[4]	"Acyl-CoA binding and acylation of UDP-glucuronosyltransferase isoforms of rat liver: their effect on enzyme activity." Yamashita A., Watanabe M., Tonegawa T., Sugiura T., Waku K. Biochem. J. 312:301-308(1995) [PubMed: 7492328] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-44, LIPID-BINDING.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J02589 Genomic DNA. Translation: AAA42314.1. M74439 Genomic DNA. No translation available. X03478 mRNA. Translation: CAA27198.1.
IPI	IPI00212110.
PIR	A40467.
UniGene	Rn.2521
3D structure databases
SMR	P08541. Positions 286-447.
ModBase	Search...
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Proteomic databases
PRIDE	P08541.
Organism-specific databases
RGD	3936. Ugt2b.
Phylogenomic databases
HOVERGEN	P08541.
Enzyme and pathway databases
BRENDA	2.4.1.17. 248.
Gene expression databases
ArrayExpress	P08541.
Family and domain databases
InterPro	IPR002213. UDP_glucos_trans. [Graphical view]
PANTHER	PTHR11926. UDP_glucos_trans. 1 hit.
Pfam	PF00201. UDPGT. 1 hit. [Graphical view]
PROSITE	PS00375. UDPGT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UD2B2_RAT

Accession

Primary (citable) accession number: P08541

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	June 16, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)