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P08538

- YOPH_YERPS

UniProt

P08538 - YOPH_YERPS

Protein

Tyrosine-protein phosphatase YopH

Gene

yopH

Organism
Yersinia pseudotuberculosis serotype I (strain IP32953)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 3 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Essential virulence determinant. This protein is a protein tyrosine phosphatase. The essential function of YopH in Yersinia pathogenesis is host-protein dephosphorylation. It contributes to the ability of the bacteria to resist phagocytosis by peritoneal macrophages.1 Publication

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 PublicationPROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei403 – 4031Phosphocysteine intermediate

    GO - Molecular functioni

    1. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. pathogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Virulence

    Enzyme and pathway databases

    BioCyciYPSE273123:GI1M-4190-MONOMER.

    Protein family/group databases

    PptaseDBiP3D0412161.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein phosphatase YopH (EC:3.1.3.48)
    Alternative name(s):
    Virulence protein
    Gene namesi
    Name:yopH
    Synonyms:yop2b
    Ordered Locus Names:pYV0094
    Encoded oniPlasmid pIB11 Publication
    Plasmid pYV1 Publication
    OrganismiYersinia pseudotuberculosis serotype I (strain IP32953)
    Taxonomic identifieri273123 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
    ProteomesiUP000001011: Plasmid pYV

    Subcellular locationi

    Secreted
    Note: Secreted via type III secretion system.

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi403 – 4031C → A: Abolishes PTPase activity and significantly reduces the virulence. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 468468Tyrosine-protein phosphatase YopHPRO_0000094862Add
    BLAST

    Expressioni

    Inductioni

    At 37 degrees Celsius in the absence of calcium.

    Interactioni

    Protein-protein interaction databases

    IntActiP08538. 1 interaction.
    STRINGi273123.pYV0094.

    Structurei

    Secondary structure

    1
    468
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 1713
    Beta strandi24 – 3411
    Helixi44 – 463
    Helixi50 – 6213
    Turni63 – 653
    Helixi70 – 8213
    Beta strandi88 – 947
    Beta strandi97 – 10610
    Helixi110 – 12415
    Beta strandi126 – 1283

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K46X-ray2.20A1-129[»]
    1M0VNMR-A1-129[»]
    ProteinModelPortaliP08538.
    SMRiP08538. Positions 1-129, 182-468.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP08538.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini152 – 461310Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG74835.
    HOGENOMiHOG000219626.
    OMAiSHVANIV.
    OrthoDBiEOG6423BT.

    Family and domain databases

    Gene3Di3.30.1570.10. 1 hit.
    3.90.190.10. 1 hit.
    InterProiIPR029021. Prot-tyrosine_phosphatase-like.
    IPR015103. ProtTyrPase_YopH_N.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    IPR003546. Tyr_Pase_SptP/YopH.
    [Graphical view]
    PfamiPF00102. Y_phosphatase. 1 hit.
    PF09013. YopH_N. 1 hit.
    [Graphical view]
    PRINTSiPR01371. BACYPHPHTASE.
    PR00700. PRTYPHPHTASE.
    SMARTiSM00194. PTPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    SSF64449. SSF64449. 1 hit.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P08538-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNLSLSDLHR QVSRLVQQES GDCTGKLRGN VAANKETTFQ GLTIASGARE    50
    SEKVFAQTVL SHVANVVLTQ EDTAKLLQST VKHNLNNYDL RSVGNGNSVL 100
    VSLRSDQMTL QDAKVLLEAA LRQESGARGH VSSHSHSALH APGTPVREGL 150
    RSHLDPRTPP LPPRERPHTS GHHGAGEARA TAPSTVSPYG PEARAELSSR 200
    LTTLRNTLAP ATNDPRYLQA CGGEKLNRFR DIQCCRQTAV RADLNANYIQ 250
    VGNTRTIACQ YPLQSQLESH FRMLAENRTP VLAVLASSSE IANQRFGMPD 300
    YFRQSGTYGS ITVESKMTQQ VGLGDGIMAD MYTLTIREAG QKTISVPVVH 350
    VGNWPDQTAV SSEVTKALAS LVDQTAETKR NMYESKGSSA VGDDSKLRPV 400
    IHCRAGVGRT AQLIGAMCMN DSRNSQLSVE DMVSQMRVQR NGIMVQKDEQ 450
    LDVLIKLAEG QGRPLLNS 468
    Length:468
    Mass (Da):50,869
    Last modified:November 23, 2004 - v3
    Checksum:i9EA639C08197EA81
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti211 – 2111A → R in CAA68629. (PubMed:2837614)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00551 Genomic DNA. Translation: CAA68629.1.
    BX936399 Genomic DNA. Translation: CAF25437.1.
    PIRiS01054.
    RefSeqiYP_068503.1. NC_006153.2.

    Genome annotation databases

    EnsemblBacteriaiCAF25437; CAF25437; pYV0094.
    GeneIDi2952987.
    KEGGiyps:pYV0094.
    PATRICi18637944. VBIYerPse22266_0099.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00551 Genomic DNA. Translation: CAA68629.1 .
    BX936399 Genomic DNA. Translation: CAF25437.1 .
    PIRi S01054.
    RefSeqi YP_068503.1. NC_006153.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K46 X-ray 2.20 A 1-129 [» ]
    1M0V NMR - A 1-129 [» ]
    ProteinModelPortali P08538.
    SMRi P08538. Positions 1-129, 182-468.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P08538. 1 interaction.
    STRINGi 273123.pYV0094.

    Chemistry

    BindingDBi P08538.
    ChEMBLi CHEMBL5835.

    Protein family/group databases

    PptaseDBi P3D0412161.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAF25437 ; CAF25437 ; pYV0094 .
    GeneIDi 2952987.
    KEGGi yps:pYV0094.
    PATRICi 18637944. VBIYerPse22266_0099.

    Phylogenomic databases

    eggNOGi NOG74835.
    HOGENOMi HOG000219626.
    OMAi SHVANIV.
    OrthoDBi EOG6423BT.

    Enzyme and pathway databases

    BioCyci YPSE273123:GI1M-4190-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P08538.

    Family and domain databases

    Gene3Di 3.30.1570.10. 1 hit.
    3.90.190.10. 1 hit.
    InterProi IPR029021. Prot-tyrosine_phosphatase-like.
    IPR015103. ProtTyrPase_YopH_N.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    IPR003546. Tyr_Pase_SptP/YopH.
    [Graphical view ]
    Pfami PF00102. Y_phosphatase. 1 hit.
    PF09013. YopH_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01371. BACYPHPHTASE.
    PR00700. PRTYPHPHTASE.
    SMARTi SM00194. PTPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    SSF64449. SSF64449. 1 hit.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The plasmid-encoded Yop2b protein of Yersinia pseudotuberculosis is a virulence determinant regulated by calcium and temperature at the level of transcription."
      Boelin I., Wolf-Watz H.
      Mol. Microbiol. 2:237-245(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: YPIII / Serotype O:3.
      Plasmid: pIB1
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: IP32953.
      Plasmid: pYV
    3. "Tyrosine phosphate hydrolysis of host proteins by an essential Yersinia virulence determinant."
      Bliska J.B., Guan K.L., Dixon J.E., Falkow S.
      Proc. Natl. Acad. Sci. U.S.A. 88:1187-1191(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS.
    4. "Structure of the type III secretion and substrate-binding domain of Yersinia YopH phosphatase."
      Smith C.L., Khandelwal P., Keliikuli K., Zuiderweg E.R., Saper M.A.
      Mol. Microbiol. 42:967-979(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 1-129.
    5. "Solution structure and phosphopeptide binding to the N-terminal domain of Yersinia YopH: comparison with a crystal structure."
      Khandelwal P., Keliikuli K., Smith C.L., Saper M.A., Zuiderweg E.R.
      Biochemistry 41:11425-11437(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 1-129.

    Entry informationi

    Entry nameiYOPH_YERPS
    AccessioniPrimary (citable) accession number: P08538
    Secondary accession number(s): Q663H2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 117 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Plasmid

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3