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Protein

Tyrosine-protein phosphatase YopH

Gene

yopH

Organism
Yersinia pseudotuberculosis serotype I (strain IP32953)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential virulence determinant. This protein is a protein tyrosine phosphatase. The essential function of YopH in Yersinia pathogenesis is host-protein dephosphorylation. It contributes to the ability of the bacteria to resist phagocytosis by peritoneal macrophages.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei403 – 4031Phosphocysteine intermediate

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. pathogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BioCyciYPSE273123:GI1M-4190-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein phosphatase YopH (EC:3.1.3.48)
Alternative name(s):
Virulence protein
Gene namesi
Name:yopH
Synonyms:yop2b
Ordered Locus Names:pYV0094
Encoded oniPlasmid pIB11 Publication
Plasmid pYV1 Publication
OrganismiYersinia pseudotuberculosis serotype I (strain IP32953)
Taxonomic identifieri273123 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000001011 Componenti: Plasmid pYV

Subcellular locationi

  1. Secreted

  2. Note: Secreted via type III secretion system.

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi403 – 4031C → A: Abolishes PTPase activity and significantly reduces the virulence. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 468468Tyrosine-protein phosphatase YopHPRO_0000094862Add
BLAST

Expressioni

Inductioni

At 37 degrees Celsius in the absence of calcium.

Interactioni

Protein-protein interaction databases

IntActiP08538. 1 interaction.
STRINGi273123.pYV0094.

Structurei

Secondary structure

1
468
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 1713Combined sources
Beta strandi24 – 3411Combined sources
Helixi44 – 463Combined sources
Helixi50 – 6213Combined sources
Turni63 – 653Combined sources
Helixi70 – 8213Combined sources
Beta strandi88 – 947Combined sources
Beta strandi97 – 10610Combined sources
Helixi110 – 12415Combined sources
Beta strandi126 – 1283Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K46X-ray2.20A1-129[»]
1M0VNMR-A1-129[»]
ProteinModelPortaliP08538.
SMRiP08538. Positions 1-129, 182-468.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08538.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini152 – 461310Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG74835.
HOGENOMiHOG000219626.
OMAiSHVANIV.
OrthoDBiEOG6423BT.

Family and domain databases

Gene3Di3.30.1570.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR015103. ProtTyrPase_YopH_N.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR003546. Tyr_Pase_SptP/YopH.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
PF09013. YopH_N. 1 hit.
[Graphical view]
PRINTSiPR01371. BACYPHPHTASE.
PR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF64449. SSF64449. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08538-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLSLSDLHR QVSRLVQQES GDCTGKLRGN VAANKETTFQ GLTIASGARE
60 70 80 90 100
SEKVFAQTVL SHVANVVLTQ EDTAKLLQST VKHNLNNYDL RSVGNGNSVL
110 120 130 140 150
VSLRSDQMTL QDAKVLLEAA LRQESGARGH VSSHSHSALH APGTPVREGL
160 170 180 190 200
RSHLDPRTPP LPPRERPHTS GHHGAGEARA TAPSTVSPYG PEARAELSSR
210 220 230 240 250
LTTLRNTLAP ATNDPRYLQA CGGEKLNRFR DIQCCRQTAV RADLNANYIQ
260 270 280 290 300
VGNTRTIACQ YPLQSQLESH FRMLAENRTP VLAVLASSSE IANQRFGMPD
310 320 330 340 350
YFRQSGTYGS ITVESKMTQQ VGLGDGIMAD MYTLTIREAG QKTISVPVVH
360 370 380 390 400
VGNWPDQTAV SSEVTKALAS LVDQTAETKR NMYESKGSSA VGDDSKLRPV
410 420 430 440 450
IHCRAGVGRT AQLIGAMCMN DSRNSQLSVE DMVSQMRVQR NGIMVQKDEQ
460
LDVLIKLAEG QGRPLLNS
Length:468
Mass (Da):50,869
Last modified:November 23, 2004 - v3
Checksum:i9EA639C08197EA81
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti211 – 2111A → R in CAA68629 (PubMed:2837614).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00551 Genomic DNA. Translation: CAA68629.1.
BX936399 Genomic DNA. Translation: CAF25437.1.
PIRiS01054.
RefSeqiYP_068503.1. NC_006153.2.

Genome annotation databases

EnsemblBacteriaiCAF25437; CAF25437; pYV0094.
KEGGiyps:pYV0094.
PATRICi18637944. VBIYerPse22266_0099.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00551 Genomic DNA. Translation: CAA68629.1.
BX936399 Genomic DNA. Translation: CAF25437.1.
PIRiS01054.
RefSeqiYP_068503.1. NC_006153.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K46X-ray2.20A1-129[»]
1M0VNMR-A1-129[»]
ProteinModelPortaliP08538.
SMRiP08538. Positions 1-129, 182-468.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP08538. 1 interaction.
STRINGi273123.pYV0094.

Chemistry

BindingDBiP08538.
ChEMBLiCHEMBL5835.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAF25437; CAF25437; pYV0094.
KEGGiyps:pYV0094.
PATRICi18637944. VBIYerPse22266_0099.

Phylogenomic databases

eggNOGiNOG74835.
HOGENOMiHOG000219626.
OMAiSHVANIV.
OrthoDBiEOG6423BT.

Enzyme and pathway databases

BioCyciYPSE273123:GI1M-4190-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP08538.
PROiP08538.

Family and domain databases

Gene3Di3.30.1570.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR015103. ProtTyrPase_YopH_N.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR003546. Tyr_Pase_SptP/YopH.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
PF09013. YopH_N. 1 hit.
[Graphical view]
PRINTSiPR01371. BACYPHPHTASE.
PR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF64449. SSF64449. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The plasmid-encoded Yop2b protein of Yersinia pseudotuberculosis is a virulence determinant regulated by calcium and temperature at the level of transcription."
    Boelin I., Wolf-Watz H.
    Mol. Microbiol. 2:237-245(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: YPIII / Serotype O:3.
    Plasmid: pIB1
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IP32953.
    Plasmid: pYV
  3. "Tyrosine phosphate hydrolysis of host proteins by an essential Yersinia virulence determinant."
    Bliska J.B., Guan K.L., Dixon J.E., Falkow S.
    Proc. Natl. Acad. Sci. U.S.A. 88:1187-1191(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS.
  4. "Structure of the type III secretion and substrate-binding domain of Yersinia YopH phosphatase."
    Smith C.L., Khandelwal P., Keliikuli K., Zuiderweg E.R., Saper M.A.
    Mol. Microbiol. 42:967-979(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 1-129.
  5. "Solution structure and phosphopeptide binding to the N-terminal domain of Yersinia YopH: comparison with a crystal structure."
    Khandelwal P., Keliikuli K., Smith C.L., Saper M.A., Zuiderweg E.R.
    Biochemistry 41:11425-11437(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 1-129.

Entry informationi

Entry nameiYOPH_YERPS
AccessioniPrimary (citable) accession number: P08538
Secondary accession number(s): Q663H2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 23, 2004
Last modified: April 29, 2015
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.