P08538 (YOPH_YERPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein phosphatase YopH
EC=3.1.3.48

Alternative name(s):
Virulence protein

Gene names

Name:	yopH
Synonyms:	yop2b
Ordered Locus Names:	pYV0094

Encoded on

Plasmid pIB1 Ref.1
Plasmid pYV Ref.2

Organism

Yersinia pseudotuberculosis serotype I (strain IP32953) [Complete proteome] [HAMAP]

Taxonomic identifier

273123 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia › Yersinia pseudotuberculosis

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential virulence determinant. This protein is a protein tyrosine phosphatase. The essential function of YopH in Yersinia pathogenesis is host-protein dephosphorylation. It contributes to the ability of the bacteria to resist phagocytosis by peritoneal macrophages. Ref.3
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. Ref.3
Subcellular location	Secreted. Note: Secreted via type III secretion system.
Induction	At 37 degrees Celsius in the absence of calcium.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Secreted
Molecular function	Hydrolase Protein phosphatase
Technical term	3D-structure Complete proteome Plasmid
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 468

468

Tyrosine-protein phosphatase YopH

PRO_0000094862

Regions

Domain

152 – 461

310

Tyrosine-protein phosphatase

Sites

Active site

403

1

Phosphocysteine intermediate

Experimental info

Mutagenesis

403

1

C → A: Abolishes PTPase activity and significantly reduces the virulence.

Sequence conflict

211

1

A → R in CAA68629. Ref.1

Secondary structure

1

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468

Helix Strand Turn

Details...

Helix

5 – 17

13

Beta strand

24 – 34

11

Helix

44 – 46

3

Helix

50 – 62

13

Turn

63 – 65

3

Helix

70 – 82

13

Beta strand

88 – 94

7

Beta strand

97 – 106

10

Helix

110 – 124

15

Beta strand

126 – 128

3

Sequences

	Sequence		Length	Mass (Da)
	P08538 [UniParc]. Last modified November 23, 2004. Version 3. Checksum: 9EA639C08197EA81	FASTA	468	50,869
	10 20 30 40 50 60 MNLSLSDLHR QVSRLVQQES GDCTGKLRGN VAANKETTFQ GLTIASGARE SEKVFAQTVL 70 80 90 100 110 120 SHVANVVLTQ EDTAKLLQST VKHNLNNYDL RSVGNGNSVL VSLRSDQMTL QDAKVLLEAA 130 140 150 160 170 180 LRQESGARGH VSSHSHSALH APGTPVREGL RSHLDPRTPP LPPRERPHTS GHHGAGEARA 190 200 210 220 230 240 TAPSTVSPYG PEARAELSSR LTTLRNTLAP ATNDPRYLQA CGGEKLNRFR DIQCCRQTAV 250 260 270 280 290 300 RADLNANYIQ VGNTRTIACQ YPLQSQLESH FRMLAENRTP VLAVLASSSE IANQRFGMPD 310 320 330 340 350 360 YFRQSGTYGS ITVESKMTQQ VGLGDGIMAD MYTLTIREAG QKTISVPVVH VGNWPDQTAV 370 380 390 400 410 420 SSEVTKALAS LVDQTAETKR NMYESKGSSA VGDDSKLRPV IHCRAGVGRT AQLIGAMCMN 430 440 450 460 DSRNSQLSVE DMVSQMRVQR NGIMVQKDEQ LDVLIKLAEG QGRPLLNS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The plasmid-encoded Yop2b protein of Yersinia pseudotuberculosis is a virulence determinant regulated by calcium and temperature at the level of transcription." Boelin I., Wolf-Watz H. Mol. Microbiol. 2:237-245(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YPIII / Serotype O:3.
[2]	"Insights into the evolution of Yersinia pestis through whole-genome comparison with Yersinia pseudotuberculosis." Chain P.S.G., Carniel E., Larimer F.W., Lamerdin J., Stoutland P.O., Regala W.M., Georgescu A.M., Vergez L.M., Land M.L., Motin V.L., Brubaker R.R., Fowler J., Hinnebusch J., Marceau M., Medigue C., Simonet M., Chenal-Francisque V., Souza B. , Dacheux D., Elliott J.M., Derbise A., Hauser L.J., Garcia E. Proc. Natl. Acad. Sci. U.S.A. 101:13826-13831(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IP32953.
[3]	"Tyrosine phosphate hydrolysis of host proteins by an essential Yersinia virulence determinant." Bliska J.B., Guan K.L., Dixon J.E., Falkow S. Proc. Natl. Acad. Sci. U.S.A. 88:1187-1191(1991) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS.
[4]	"Structure of the type III secretion and substrate-binding domain of Yersinia YopH phosphatase." Smith C.L., Khandelwal P., Keliikuli K., Zuiderweg E.R., Saper M.A. Mol. Microbiol. 42:967-979(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 1-129.
[5]	"Solution structure and phosphopeptide binding to the N-terminal domain of Yersinia YopH: comparison with a crystal structure." Khandelwal P., Keliikuli K., Smith C.L., Saper M.A., Zuiderweg E.R. Biochemistry 41:11425-11437(2002) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 1-129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y00551 Genomic DNA. Translation: CAA68629.1.
BX936399 Genomic DNA. Translation: CAF25437.1.

PIR

S01054.

RefSeq

YP_068503.1. NC_006153.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K46	X-ray	2.20	A	1-129	[»]
1M0V	NMR	-	A	1-129	[»]

ProteinModelPortal

P08538.

SMR

P08538. Positions 1-129, 182-468.

ModBase

Search...

Protein-protein interaction databases

IntAct

P08538. 1 interaction.

STRING

273123.pYV0094.

Protein family/group databases

PptaseDB

P3D0412161.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

CAF25437; CAF25437; pYV0094.

GeneID

2952987.

KEGG

yps:pYV0094.

PATRIC

18637944. VBIYerPse22266_0099.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

NOG74835.

HOGENOM

HOG000219626.

OMA

SHVANIV.

ProtClustDB

CLSK862151.

Family and domain databases

Gene3D

3.30.1570.10. 1 hit.

InterPro

IPR015103. ProtTyrPase_YopH_N.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR003546. Tyr_Pase_SptP/YopH.
[Graphical view]

Pfam

PF00102. Y_phosphatase. 1 hit.
PF09013. YopH_N. 1 hit.
[Graphical view]

PRINTS

PR01371. BACYPHPHTASE.
PR00700. PRTYPHPHTASE.

SMART

SM00194. PTPc. 1 hit.
[Graphical view]

SUPFAM

SSF64449. ProtTyrPase_YopH_N. 1 hit.

PROSITE

PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P08538.

ChEMBL

CHEMBL5835.

EvolutionaryTrace

P08538.

Entry information

Entry name

YOPH_YERPS

Accession

Primary (citable) accession number: P08538
Secondary accession number(s): Q663H2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	November 23, 2004
Last modified:	May 1, 2013
This is version 109 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program