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Protein

Tyrosine-protein phosphatase YopH

Gene

yopH

Organism
Yersinia pseudotuberculosis serotype I (strain IP32953)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential virulence determinant. This protein is a protein tyrosine phosphatase. The essential function of YopH in Yersinia pathogenesis is host-protein dephosphorylation. It contributes to the ability of the bacteria to resist phagocytosis by peritoneal macrophages.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei403Phosphocysteine intermediate1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein phosphatase YopH (EC:3.1.3.48)
Alternative name(s):
Virulence protein
Gene namesi
Name:yopH
Synonyms:yop2b
Ordered Locus Names:pYV0094
Encoded oniPlasmid pIB11 Publication
Plasmid pYV1 Publication
OrganismiYersinia pseudotuberculosis serotype I (strain IP32953)
Taxonomic identifieri273123 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000001011 Componenti: Plasmid pYV

Subcellular locationi

  • Secreted

  • Note: Secreted via type III secretion system.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi403C → A: Abolishes PTPase activity and significantly reduces the virulence. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL5835.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000948621 – 468Tyrosine-protein phosphatase YopHAdd BLAST468

Proteomic databases

PRIDEiP08538.

Expressioni

Inductioni

At 37 degrees Celsius in the absence of calcium.

Interactioni

Protein-protein interaction databases

IntActiP08538. 1 interactor.

Structurei

Secondary structure

1468
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 17Combined sources13
Beta strandi24 – 34Combined sources11
Helixi44 – 46Combined sources3
Helixi50 – 62Combined sources13
Turni63 – 65Combined sources3
Helixi70 – 82Combined sources13
Beta strandi88 – 94Combined sources7
Beta strandi97 – 106Combined sources10
Helixi110 – 124Combined sources15
Beta strandi126 – 128Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K46X-ray2.20A1-129[»]
1M0VNMR-A1-129[»]
ProteinModelPortaliP08538.
SMRiP08538.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08538.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini152 – 461Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST310

Sequence similaritiesi

Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000219626.
OMAiSHVANIV.

Family and domain databases

Gene3Di3.30.1570.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR015103. ProtTyrPase_YopH_N.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR003546. Tyr_Pase_SptP/YopH.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
PF09013. YopH_N. 1 hit.
[Graphical view]
PRINTSiPR01371. BACYPHPHTASE.
PR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF64449. SSF64449. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08538-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLSLSDLHR QVSRLVQQES GDCTGKLRGN VAANKETTFQ GLTIASGARE
60 70 80 90 100
SEKVFAQTVL SHVANVVLTQ EDTAKLLQST VKHNLNNYDL RSVGNGNSVL
110 120 130 140 150
VSLRSDQMTL QDAKVLLEAA LRQESGARGH VSSHSHSALH APGTPVREGL
160 170 180 190 200
RSHLDPRTPP LPPRERPHTS GHHGAGEARA TAPSTVSPYG PEARAELSSR
210 220 230 240 250
LTTLRNTLAP ATNDPRYLQA CGGEKLNRFR DIQCCRQTAV RADLNANYIQ
260 270 280 290 300
VGNTRTIACQ YPLQSQLESH FRMLAENRTP VLAVLASSSE IANQRFGMPD
310 320 330 340 350
YFRQSGTYGS ITVESKMTQQ VGLGDGIMAD MYTLTIREAG QKTISVPVVH
360 370 380 390 400
VGNWPDQTAV SSEVTKALAS LVDQTAETKR NMYESKGSSA VGDDSKLRPV
410 420 430 440 450
IHCRAGVGRT AQLIGAMCMN DSRNSQLSVE DMVSQMRVQR NGIMVQKDEQ
460
LDVLIKLAEG QGRPLLNS
Length:468
Mass (Da):50,869
Last modified:November 23, 2004 - v3
Checksum:i9EA639C08197EA81
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti211A → R in CAA68629 (PubMed:2837614).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00551 Genomic DNA. Translation: CAA68629.1.
BX936399 Genomic DNA. Translation: CAF25437.1.
PIRiS01054.
RefSeqiWP_002213278.1. NZ_CP009711.1.

Genome annotation databases

EnsemblBacteriaiCAF25437; CAF25437; pYV0094.
KEGGiypo:BZ17_4241.
yps:pYV0094.
PATRICi18637944. VBIYerPse22266_0099.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00551 Genomic DNA. Translation: CAA68629.1.
BX936399 Genomic DNA. Translation: CAF25437.1.
PIRiS01054.
RefSeqiWP_002213278.1. NZ_CP009711.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K46X-ray2.20A1-129[»]
1M0VNMR-A1-129[»]
ProteinModelPortaliP08538.
SMRiP08538.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP08538. 1 interactor.

Chemistry databases

ChEMBLiCHEMBL5835.

Proteomic databases

PRIDEiP08538.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAF25437; CAF25437; pYV0094.
KEGGiypo:BZ17_4241.
yps:pYV0094.
PATRICi18637944. VBIYerPse22266_0099.

Phylogenomic databases

HOGENOMiHOG000219626.
OMAiSHVANIV.

Miscellaneous databases

EvolutionaryTraceiP08538.
PROiP08538.

Family and domain databases

Gene3Di3.30.1570.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR015103. ProtTyrPase_YopH_N.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR003546. Tyr_Pase_SptP/YopH.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
PF09013. YopH_N. 1 hit.
[Graphical view]
PRINTSiPR01371. BACYPHPHTASE.
PR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF64449. SSF64449. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYOPH_YERPS
AccessioniPrimary (citable) accession number: P08538
Secondary accession number(s): Q663H2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 23, 2004
Last modified: November 2, 2016
This is version 134 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.