Cytochrome b-c1 complex subunit 8 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P08525 (QCR8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 118. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cytochrome b-c1 complex subunit 8

Alternative name(s):
Complex III subunit 8
Complex III subunit VII
Ubiquinol-cytochrome c reductase complex 11 kDa protein
Ubiquinol-cytochrome c reductase complex ubiquinone-binding protein QP-C

Gene names

Name:	QCR8
Ordered Locus Names:	YJL166W
ORF Names:	J0526

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	94 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The complex couples electron transfer from ubiquinol to cytochrome c. QCR8, together with cytochrome b, binds to ubiquinone.
Subunit structure	Fungal cytochrome b-c1 complex contains 10 subunits; 3 respiratory subunits, 2 core proteins and 5 low-molecular weight proteins. Cytochrome b-c1 complex is a homodimer.
Subcellular location	Mitochondrion inner membrane.
Miscellaneous	Present with 6140 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the UQCRQ/QCR8 family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aerobic respiration Inferred from mutant phenotype Ref.2. Source: SGD mitochondrial electron transport, ubiquinol to cytochrome c Inferred from mutant phenotype Ref.2. Source: SGD
Cellular component	mitochondrial respiratory chain complex III Inferred from direct assay Ref.7. Source: SGD
Molecular function	ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 94

Cytochrome b-c1 complex subunit 8

PRO_0000193551

Secondary structure

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P08525 [UniParc].

Last modified October 1, 1993. Version 2.
Checksum: 9CB828E495F6ED9E
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FASTA

10,975

        10         20         30         40         50         60 
MGPPSGKTYM GWWGHMGGPK QKGITSYAVS PYAQKPLQGI FHNAVFNSFR RFKSQFLYVL 

        70         80         90 
IPAGIYWYWW KNGNEYNEFL YSKAGREELE RVNV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the gene encoding the 11-kDa subunit of the ubiquinol-cytochrome-c oxidoreductase in Saccharomyces cerevisiae." Maarse A.C., Grivell L.A. Eur. J. Biochem. 165:419-425(1987) [PubMed: 3036507] [Abstract] Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE.
[2]	"A region of the C-terminal part of the 11-kDa subunit of ubiquinol-cytochrome-c oxidoreductase of the yeast Saccharomyces cerevisiae contributes to the structure of the Qout reaction domain." Hemrika W., Berden J.A., Grivell L.A. Eur. J. Biochem. 215:601-609(1993) [PubMed: 8394810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Structure at 2.3 A resolution of the cytochrome bc1 complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment." Hunte C., Koepke J., Lange C., Rossmanith T., Michel H. Structure 8:669-684(2000) [PubMed: 10873857] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[8]	"Crystal structure of the yeast cytochrome bc1 complex with its bound substrate cytochrome c." Lange C., Hunte C. Proc. Natl. Acad. Sci. U.S.A. 99:2800-2805(2002) [PubMed: 11880631] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.97 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X05550 Genomic DNA. Translation: CAA29065.1.
Z49441 Genomic DNA. Translation: CAA89461.1.
AY558554 Genomic DNA. Translation: AAS56880.1.
BK006943 Genomic DNA. Translation: DAA08637.1.

PIR

S48138.

RefSeq

NP_012369.1. NM_001181599.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EZV	X-ray	2.30	G	2-94	[»]
1KB9	X-ray	2.30	H	2-94	[»]
1KYO	X-ray	2.97	H/S	2-94	[»]
1P84	X-ray	2.50	H	2-94	[»]
2IBZ	X-ray	2.30	G	1-94	[»]
3CX5	X-ray	1.90	H/S	2-94	[»]
3CXH	X-ray	2.50	H/S	2-94	[»]

ProteinModelPortal

P08525.

SMR

P08525. Positions 2-94.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-4706N.

IntAct

P08525. 2 interactions.

MINT

MINT-572396.

STRING

P08525.

Proteomic databases

PeptideAtlas

P08525.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YJL166W; YJL166W; YJL166W.

GeneID

853273.

KEGG

sce:YJL166W.

NMPDR

fig|4932.3.peg.3333.

Organism-specific databases

CYGD

YJL166w.

SGD

S000003702. QCR8.

Phylogenomic databases

eggNOG

fuNOG10903.

GeneTree

EFGT00050000005866.

HOGENOM

HBG333553.

OMA

TKAGREE.

OrthoDB

EOG4XPTRM.

Gene expression databases

ArrayExpress

P08525.

Genevestigator

P08525.

GermOnline

YJL166W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR004205. UcrQ.
[Graphical view]

Gene3D

G3DSA:1.20.5.210. UcrQ. 1 hit.

K00418.

Pfam

PF02939. UcrQ. 1 hit.
[Graphical view]

ProDom

PD331499. UcrQ. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF81508. UcrQ. 1 hit.

ProtoNet

Search...

Other

NextBio

973550.

Entry information

Entry name

QCR8_YEAST

Accession

Primary (citable) accession number: P08525
Secondary accession number(s): D6VW21

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents