Cytochrome b-c1 complex subunit 8 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P08525 (QCR8_YEAST)

Last modified November 24, 2009. Version 99. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Cytochrome b-c1 complex subunit 8
Alternative name(s):
    Ubiquinol-cytochrome c reductase complex ubiquinone-binding protein QP-C
    Ubiquinol-cytochrome c reductase complex 11 kDa protein
    Complex III subunit 8
    Complex III subunit VII

Gene names

Name:	QCR8
Ordered Locus Names:	YJL166W
ORF Names:	J0526

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	94 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The complex couples electron transfer from ubiquinol to cytochrome c. QCR8, together with cytochrome b, binds to ubiquinone.
Subunit structure	Fungal cytochrome b-c1 complex contains 10 subunits; 3 respiratory subunits, 2 core proteins and 5 low-molecular weight proteins. Cytochrome b-c1 complex is a homodimer.
Subcellular location	Mitochondrion inner membrane.
Miscellaneous	Present with 6140 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the UQCRQ/QCR8 family.

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Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	aerobic respiration Ref.2 Inferred from mutant phenotype. Source: SGD mitochondrial electron transport, ubiquinol to cytochrome c Ref.2 Inferred from mutant phenotype. Source: SGD transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial respiratory chain complex III Ref.6 Inferred from direct assay. Source: SGD
Molecular function	ubiquinol-cytochrome-c reductase activity Ref.2 Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 94

Cytochrome b-c1 complex subunit 8

PRO_0000193551

Secondary structure

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P08525-1 [UniParc].

Last modified October 1, 1993. Version 2.
Checksum: 9CB828E495F6ED9E
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FASTA

10,975

        10         20         30         40         50         60 
MGPPSGKTYM GWWGHMGGPK QKGITSYAVS PYAQKPLQGI FHNAVFNSFR RFKSQFLYVL 

        70         80         90 
IPAGIYWYWW KNGNEYNEFL YSKAGREELE RVNV

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the gene encoding the 11-kDa subunit of the ubiquinol-cytochrome-c oxidoreductase in Saccharomyces cerevisiae." Maarse A.C., Grivell L.A. Eur. J. Biochem. 165:419-425(1987) [PubMed: 3036507] [Abstract] Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE.
[2]	"A region of the C-terminal part of the 11-kDa subunit of ubiquinol-cytochrome-c oxidoreductase of the yeast Saccharomyces cerevisiae contributes to the structure of the Qout reaction domain." Hemrika W., Berden J.A., Grivell L.A. Eur. J. Biochem. 215:601-609(1993) [PubMed: 8394810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Structure at 2.3 A resolution of the cytochrome bc1 complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment." Hunte C., Koepke J., Lange C., Rossmanith T., Michel H. Structure 8:669-684(2000) [PubMed: 10873857] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[7]	"Crystal structure of the yeast cytochrome bc1 complex with its bound substrate cytochrome c." Lange C., Hunte C. Proc. Natl. Acad. Sci. U.S.A. 99:2800-2805(2002) [PubMed: 11880631] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.97 ANGSTROMS).
+	Additional computationally mapped references.

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Cross-references

Sequence databases

X05550 Genomic DNA. Translation: CAA29065.1.
Z49441 Genomic DNA. Translation: CAA89461.1.
AY558554 Genomic DNA. Translation: AAS56880.1.

PIR

S48138.

RefSeq

NP_012369.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EZV	X-ray	2.30	G	2-94	[»]
1KB9	X-ray	2.30	H	2-94	[»]
1KYO	X-ray	2.97	H/S	2-94	[»]
1P84	X-ray	2.50	H	2-94	[»]
2IBZ	X-ray	2.30	G	1-94	[»]
3CX5	X-ray	1.90	H/S	2-94	[»]
3CXH	X-ray	2.50	H/S	2-94	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:4706N.

IntAct

P08525. 3 interactions.

STRING

P08525.

Proteomic databases

PeptideAtlas

P08525.

PRIDE

P08525.

Genome annotation databases

Ensembl

YJL166W; YJL166W; YJL166W; Saccharomyces cerevisiae. [Genome view]

GeneID

853273.

KEGG

sce:YJL166W.

NMPDR

fig|4932.3.peg.3333.

Organism-specific databases

CYGD

YJL166w.

SGD

S000003702. QCR8.

Phylogenomic databases

HOGENOM

P08525.

OMA

TKAGREE

OrthoDB

EOG9J6T72

Gene expression databases

ArrayExpress

P08525.

Genevestigator

P08525.

GermOnline

YJL166W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR004205. UcrQ.
[Graphical view]

Gene3D

G3DSA:1.20.5.210. UcrQ. 1 hit.

Pfam

PF02939. UcrQ. 1 hit.
[Graphical view]

ProDom

PD331499. UcrQ. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Other Resources

NextBio

973550.

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Entry information

Entry name

QCR8_YEAST

Accession

Primary (citable) accession number: P08525

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	October 1, 1993
Last modified:	November 24, 2009
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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