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P08524

- FPPS_YEAST

UniProt

P08524 - FPPS_YEAST

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Protein

Farnesyl pyrophosphate synthase

Gene

ERG20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate.

Catalytic activityi

Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate.
Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate.

Cofactori

Mg2+By similarityNote: Binds 3 Mg(2+) ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521Isopentenyl diphosphateBy similarity
Binding sitei55 – 551Isopentenyl diphosphateBy similarity
Binding sitei93 – 931Isopentenyl diphosphateBy similarity
Metal bindingi100 – 1001Magnesium 1By similarity
Metal bindingi100 – 1001Magnesium 2By similarity
Metal bindingi104 – 1041Magnesium 1By similarity
Metal bindingi104 – 1041Magnesium 2By similarity
Binding sitei109 – 1091Dimethylallyl diphosphateBy similarity
Binding sitei110 – 1101Isopentenyl diphosphateBy similarity
Binding sitei197 – 1971Dimethylallyl diphosphateBy similarity
Binding sitei198 – 1981Dimethylallyl diphosphateBy similarity
Binding sitei237 – 2371Dimethylallyl diphosphateBy similarity
Metal bindingi240 – 2401Magnesium 3By similarity
Binding sitei254 – 2541Dimethylallyl diphosphateBy similarity
Binding sitei263 – 2631Dimethylallyl diphosphateBy similarity

GO - Molecular functioni

  1. dimethylallyltranstransferase activity Source: SGD
  2. geranyltranstransferase activity Source: SGD
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. ergosterol biosynthetic process Source: SGD
  2. farnesyl diphosphate biosynthetic process Source: SGD
  3. geranyl diphosphate biosynthetic process Source: UniProtKB-UniPathway
  4. isoprenoid biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Isoprene biosynthesis, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-655.
YEAST:MONOMER-655.
ReactomeiREACT_188982. Activation of gene expression by SREBF (SREBP).
REACT_253879. Cholesterol biosynthesis.
UniPathwayiUPA00259; UER00368.
UPA00260; UER00369.

Names & Taxonomyi

Protein namesi
Recommended name:
Farnesyl pyrophosphate synthase (EC:2.5.1.10)
Short name:
FPP synthase
Short name:
FPS
Alternative name(s):
(2E,6E)-farnesyl diphosphate synthase
Dimethylallyltranstransferase (EC:2.5.1.1)
Farnesyl diphosphate synthase
Geranyltranstransferase
Gene namesi
Name:ERG20
Synonyms:BOT3, FDS1, FPP1
Ordered Locus Names:YJL167W
ORF Names:J0525
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL167w.
SGDiS000003703. ERG20.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. endoplasmic reticulum Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi197 – 1971K → E in ERG20-2; 14-fold decrease in FPPS activity.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 352352Farnesyl pyrophosphate synthasePRO_0000123952Add
BLAST

Proteomic databases

MaxQBiP08524.
PaxDbiP08524.
PeptideAtlasiP08524.
PRIDEiP08524.

Expressioni

Gene expression databases

GenevestigatoriP08524.

Interactioni

Protein-protein interaction databases

BioGridi33592. 54 interactions.
DIPiDIP-1163N.
IntActiP08524. 3 interactions.
MINTiMINT-555012.
STRINGi4932.YJL167W.

Structurei

3D structure databases

ProteinModelPortaliP08524.
SMRiP08524. Positions 11-352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FPP/GGPP synthase family.Curated

Phylogenomic databases

eggNOGiCOG0142.
GeneTreeiENSGT00530000064127.
HOGENOMiHOG000160912.
InParanoidiP08524.
KOiK00787.
OMAiLEACYGR.
OrthoDBiEOG79GTJ1.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR000092. Polyprenyl_synt.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF00348. polyprenyl_synt. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS00723. POLYPRENYL_SYNTHASE_1. 1 hit.
PS00444. POLYPRENYL_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08524-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASEKEIRRE RFLNVFPKLV EELNASLLAY GMPKEACDWY AHSLNYNTPG
60 70 80 90 100
GKLNRGLSVV DTYAILSNKT VEQLGQEEYE KVAILGWCIE LLQAYFLVAD
110 120 130 140 150
DMMDKSITRR GQPCWYKVPE VGEIAINDAF MLEAAIYKLL KSHFRNEKYY
160 170 180 190 200
IDITELFHEV TFQTELGQLM DLITAPEDKV DLSKFSLKKH SFIVTFKTAY
210 220 230 240 250
YSFYLPVALA MYVAGITDEK DLKQARDVLI PLGEYFQIQD DYLDCFGTPE
260 270 280 290 300
QIGKIGTDIQ DNKCSWVINK ALELASAEQR KTLDENYGKK DSVAEAKCKK
310 320 330 340 350
IFNDLKIEQL YHEYEESIAK DLKAKISQVD ESRGFKADVL TAFLNKVYKR

SK
Length:352
Mass (Da):40,483
Last modified:November 1, 1991 - v2
Checksum:i79A357BB7BFCEDDA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05091 Genomic DNA. Translation: AAA34606.1.
Z49442 Genomic DNA. Translation: CAA89462.1.
X05550 Genomic DNA. Translation: CAA29064.1.
BK006943 Genomic DNA. Translation: DAA08636.1.
PIRiA34441.
RefSeqiNP_012368.1. NM_001181600.1.

Genome annotation databases

EnsemblFungiiYJL167W; YJL167W; YJL167W.
GeneIDi853272.
KEGGisce:YJL167W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05091 Genomic DNA. Translation: AAA34606.1 .
Z49442 Genomic DNA. Translation: CAA89462.1 .
X05550 Genomic DNA. Translation: CAA29064.1 .
BK006943 Genomic DNA. Translation: DAA08636.1 .
PIRi A34441.
RefSeqi NP_012368.1. NM_001181600.1.

3D structure databases

ProteinModelPortali P08524.
SMRi P08524. Positions 11-352.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33592. 54 interactions.
DIPi DIP-1163N.
IntActi P08524. 3 interactions.
MINTi MINT-555012.
STRINGi 4932.YJL167W.

Proteomic databases

MaxQBi P08524.
PaxDbi P08524.
PeptideAtlasi P08524.
PRIDEi P08524.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL167W ; YJL167W ; YJL167W .
GeneIDi 853272.
KEGGi sce:YJL167W.

Organism-specific databases

CYGDi YJL167w.
SGDi S000003703. ERG20.

Phylogenomic databases

eggNOGi COG0142.
GeneTreei ENSGT00530000064127.
HOGENOMi HOG000160912.
InParanoidi P08524.
KOi K00787.
OMAi LEACYGR.
OrthoDBi EOG79GTJ1.

Enzyme and pathway databases

UniPathwayi UPA00259 ; UER00368 .
UPA00260 ; UER00369 .
BioCyci MetaCyc:MONOMER-655.
YEAST:MONOMER-655.
Reactomei REACT_188982. Activation of gene expression by SREBF (SREBP).
REACT_253879. Cholesterol biosynthesis.

Miscellaneous databases

NextBioi 973547.
PROi P08524.

Gene expression databases

Genevestigatori P08524.

Family and domain databases

Gene3Di 1.10.600.10. 1 hit.
InterProi IPR000092. Polyprenyl_synt.
IPR008949. Terpenoid_synth.
[Graphical view ]
Pfami PF00348. polyprenyl_synt. 1 hit.
[Graphical view ]
SUPFAMi SSF48576. SSF48576. 1 hit.
PROSITEi PS00723. POLYPRENYL_SYNTHASE_1. 1 hit.
PS00444. POLYPRENYL_SYNTHASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Farnesyl diphosphate synthetase. Molecular cloning, sequence, and expression of an essential gene from Saccharomyces cerevisiae."
    Anderson M.S., Yarger J.G., Burck C.L., Poulter C.D.
    J. Biol. Chem. 264:19176-19184(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Nucleotide sequence of the gene encoding the 11-kDa subunit of the ubiquinol-cytochrome-c oxidoreductase in Saccharomyces cerevisiae."
    Maarse A.C., Grivell L.A.
    Eur. J. Biochem. 165:419-425(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 131-352.
  5. "Characterization of a lysine-to-glutamic acid mutation in a conservative sequence of farnesyl diphosphate synthase from Saccharomyces cerevisiae."
    Blanchard L., Karst F.
    Gene 125:185-189(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTANT GLU-197.
    Strain: LB25.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFPPS_YEAST
AccessioniPrimary (citable) accession number: P08524
Secondary accession number(s): D6VW20, P15495
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1991
Last modified: November 26, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3