Reviewed, UniProtKB/Swiss-Prot P08524 (FPPS_YEAST)

Names and origin

Protein names

Recommended name:
    Farnesyl pyrophosphate synthetase
      Short name=FPP synthetase
      Short name=FPS
Alternative name(s):
    Farnesyl diphosphate synthetase
Including the following 2 domains:
    1- Recommended name:
            Dimethylallyltranstransferase
              EC=2.5.1.1
    2- Recommended name:
            Geranyltranstransferase
              EC=2.5.1.10

Gene names

Name:	FPP1
Synonyms:	BOT3, ERG20, FDS1
Ordered Locus Names:	YJL167W
ORF Names:	J0525

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate.
Catalytic activity	Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate. Geranyl diphosphate + isopentenyl diphosphate = diphosphate + trans,trans-farnesyl diphosphate.
Pathway	Isoprenoid biosynthesis; farnesyl-PP biosynthesis; farnesyl-PP from geranyl-PP and isopentenyl-PP: step 1/1. Isoprenoid biosynthesis; geranyl-PP biosynthesis; geranyl-PP from dimethylallyl-PP and isopentenyl-PP: step 1/1.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the FPP/GGPP synthetase family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Isoprene biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	farnesyl diphosphate biosynthetic process Ref.1 Inferred from direct assay. Source: SGD
Molecular function	dimethylallyltranstransferase activity Ref.1 Inferred from direct assay. Source: SGD geranyltranstransferase activity Ref.1 Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AUS1	Q08409	1	EBI-7069,EBI-35723
SSM4	P40318	1	EBI-7069,EBI-18208

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 352	352	Farnesyl pyrophosphate synthetase
Sites
	Active site	189	1	By similarity
Experimental info
	Mutagenesis	197	1	K → E in ERG20-2; 14-fold decrease in FPPS activity

Sequences

	Sequence		Length	Mass (Da)
	P08524-1 [UniParc]. Last modified November 1, 1991. Version 2. Checksum: 79A357BB7BFCEDDA	FASTA	352	40,483
	10 20 30 40 50 60 MASEKEIRRE RFLNVFPKLV EELNASLLAY GMPKEACDWY AHSLNYNTPG GKLNRGLSVV 70 80 90 100 110 120 DTYAILSNKT VEQLGQEEYE KVAILGWCIE LLQAYFLVAD DMMDKSITRR GQPCWYKVPE 130 140 150 160 170 180 VGEIAINDAF MLEAAIYKLL KSHFRNEKYY IDITELFHEV TFQTELGQLM DLITAPEDKV 190 200 210 220 230 240 DLSKFSLKKH SFIVTFKTAY YSFYLPVALA MYVAGITDEK DLKQARDVLI PLGEYFQIQD 250 260 270 280 290 300 DYLDCFGTPE QIGKIGTDIQ DNKCSWVINK ALELASAEQR KTLDENYGKK DSVAEAKCKK 310 320 330 340 350 IFNDLKIEQL YHEYEESIAK DLKAKISQVD ESRGFKADVL TAFLNKVYKR SK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Farnesyl diphosphate synthetase. Molecular cloning, sequence, and expression of an essential gene from Saccharomyces cerevisiae." Anderson M.S., Yarger J.G., Burck C.L., Poulter C.D. J. Biol. Chem. 264:19176-19184(1989) [PubMed: 2681213] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Nucleotide sequence of the gene encoding the 11-kDa subunit of the ubiquinol-cytochrome-c oxidoreductase in Saccharomyces cerevisiae." Maarse A.C., Grivell L.A. Eur. J. Biochem. 165:419-425(1987) [PubMed: 3036507] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 131-352.
[4]	"Characterization of a lysine-to-glutamic acid mutation in a conservative sequence of farnesyl diphosphate synthase from Saccharomyces cerevisiae." Blanchard L., Karst F. Gene 125:185-189(1993) [PubMed: 8096487] [Abstract] Cited for: MUTANT GLU-197. Strain: LB25.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J05091 Genomic DNA. Translation: AAA34606.1. Z49442 Genomic DNA. Translation: CAA89462.1. X05550 Genomic DNA. Translation: CAA29064.1.
PIR	A34441.
RefSeq	NP_012368.1.
3D structure databases
HSSP	HSSP built from PDB template 1FPS based on UniProtKB P08836.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1163N.
IntAct	P08524.
Proteomic databases
PeptideAtlas	P08524.
Genome annotation databases
Ensembl	YJL167W. Saccharomyces cerevisiae. [Contig view]
GeneID	853272.
GenomeReviews	Gene locus YJL167W in contig Y13136_GR.
KEGG	sce:YJL167W.
NMPDR	fig|4932.3.peg.3332.
Organism-specific databases
CYGD	YJL167w.
SGD	S000003703. ERG20.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P08524.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-655.
Gene expression databases
ArrayExpress	P08524.
GermOnline	YJL167W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000092. Polyprenyl_synt. IPR008949. Terpenoid_synth. [Graphical view]
Gene3D	G3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
Pfam	PF00348. polyprenyl_synt. 1 hit. [Graphical view]
PROSITE	PS00723. POLYPRENYL_SYNTHET_1. 1 hit. PS00444. POLYPRENYL_SYNTHET_2. 1 hit. [Graphical view]
ProDom	P08524. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P08524.
ProtoNet	Search...

Entry information

Entry name

FPPS_YEAST

Accession

Primary (citable) accession number: P08524
Secondary accession number(s): P15495

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	November 1, 1991
Last modified:	July 22, 2008
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)