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Protein

Farnesyl pyrophosphate synthase

Gene

ERG20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate.

Catalytic activityi

Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate.
Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate.

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521Isopentenyl diphosphateBy similarity
Binding sitei55 – 551Isopentenyl diphosphateBy similarity
Binding sitei93 – 931Isopentenyl diphosphateBy similarity
Metal bindingi100 – 1001Magnesium 1By similarity
Metal bindingi100 – 1001Magnesium 2By similarity
Metal bindingi104 – 1041Magnesium 1By similarity
Metal bindingi104 – 1041Magnesium 2By similarity
Binding sitei109 – 1091Dimethylallyl diphosphateBy similarity
Binding sitei110 – 1101Isopentenyl diphosphateBy similarity
Binding sitei197 – 1971Dimethylallyl diphosphateBy similarity
Binding sitei198 – 1981Dimethylallyl diphosphateBy similarity
Binding sitei237 – 2371Dimethylallyl diphosphateBy similarity
Metal bindingi240 – 2401Magnesium 3By similarity
Binding sitei254 – 2541Dimethylallyl diphosphateBy similarity
Binding sitei263 – 2631Dimethylallyl diphosphateBy similarity

GO - Molecular functioni

  1. dimethylallyltranstransferase activity Source: SGD
  2. geranyltranstransferase activity Source: SGD
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. ergosterol biosynthetic process Source: SGD
  2. farnesyl diphosphate biosynthetic process Source: SGD
  3. geranyl diphosphate biosynthetic process Source: UniProtKB-UniPathway
  4. isoprenoid biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Isoprene biosynthesis, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-655.
YEAST:MONOMER-655.
BRENDAi2.5.1.10. 984.
ReactomeiREACT_272228. Activation of gene expression by SREBF (SREBP).
REACT_283157. Cholesterol biosynthesis.
UniPathwayiUPA00259; UER00368.
UPA00260; UER00369.

Names & Taxonomyi

Protein namesi
Recommended name:
Farnesyl pyrophosphate synthase (EC:2.5.1.10)
Short name:
FPP synthase
Short name:
FPS
Alternative name(s):
(2E,6E)-farnesyl diphosphate synthase
Dimethylallyltranstransferase (EC:2.5.1.1)
Farnesyl diphosphate synthase
Geranyltranstransferase
Gene namesi
Name:ERG20
Synonyms:BOT3, FDS1, FPP1
Ordered Locus Names:YJL167W
ORF Names:J0525
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome X

Organism-specific databases

CYGDiYJL167w.
SGDiS000003703. ERG20.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. endoplasmic reticulum Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi197 – 1971K → E in ERG20-2; 14-fold decrease in FPPS activity.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 352352Farnesyl pyrophosphate synthasePRO_0000123952Add
BLAST

Proteomic databases

MaxQBiP08524.
PaxDbiP08524.
PeptideAtlasiP08524.
PRIDEiP08524.

Expressioni

Gene expression databases

GenevestigatoriP08524.

Interactioni

Protein-protein interaction databases

BioGridi33592. 54 interactions.
DIPiDIP-1163N.
IntActiP08524. 3 interactions.
MINTiMINT-555012.
STRINGi4932.YJL167W.

Structurei

3D structure databases

ProteinModelPortaliP08524.
SMRiP08524. Positions 11-352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FPP/GGPP synthase family.Curated

Phylogenomic databases

eggNOGiCOG0142.
GeneTreeiENSGT00530000064127.
HOGENOMiHOG000160912.
InParanoidiP08524.
KOiK00787.
OMAiCNGTHSK.
OrthoDBiEOG79GTJ1.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR000092. Polyprenyl_synt.
[Graphical view]
PfamiPF00348. polyprenyl_synt. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS00723. POLYPRENYL_SYNTHASE_1. 1 hit.
PS00444. POLYPRENYL_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08524-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASEKEIRRE RFLNVFPKLV EELNASLLAY GMPKEACDWY AHSLNYNTPG
60 70 80 90 100
GKLNRGLSVV DTYAILSNKT VEQLGQEEYE KVAILGWCIE LLQAYFLVAD
110 120 130 140 150
DMMDKSITRR GQPCWYKVPE VGEIAINDAF MLEAAIYKLL KSHFRNEKYY
160 170 180 190 200
IDITELFHEV TFQTELGQLM DLITAPEDKV DLSKFSLKKH SFIVTFKTAY
210 220 230 240 250
YSFYLPVALA MYVAGITDEK DLKQARDVLI PLGEYFQIQD DYLDCFGTPE
260 270 280 290 300
QIGKIGTDIQ DNKCSWVINK ALELASAEQR KTLDENYGKK DSVAEAKCKK
310 320 330 340 350
IFNDLKIEQL YHEYEESIAK DLKAKISQVD ESRGFKADVL TAFLNKVYKR

SK
Length:352
Mass (Da):40,483
Last modified:November 1, 1991 - v2
Checksum:i79A357BB7BFCEDDA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05091 Genomic DNA. Translation: AAA34606.1.
Z49442 Genomic DNA. Translation: CAA89462.1.
X05550 Genomic DNA. Translation: CAA29064.1.
BK006943 Genomic DNA. Translation: DAA08636.1.
PIRiA34441.
RefSeqiNP_012368.1. NM_001181600.1.

Genome annotation databases

EnsemblFungiiYJL167W; YJL167W; YJL167W.
GeneIDi853272.
KEGGisce:YJL167W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05091 Genomic DNA. Translation: AAA34606.1.
Z49442 Genomic DNA. Translation: CAA89462.1.
X05550 Genomic DNA. Translation: CAA29064.1.
BK006943 Genomic DNA. Translation: DAA08636.1.
PIRiA34441.
RefSeqiNP_012368.1. NM_001181600.1.

3D structure databases

ProteinModelPortaliP08524.
SMRiP08524. Positions 11-352.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33592. 54 interactions.
DIPiDIP-1163N.
IntActiP08524. 3 interactions.
MINTiMINT-555012.
STRINGi4932.YJL167W.

Proteomic databases

MaxQBiP08524.
PaxDbiP08524.
PeptideAtlasiP08524.
PRIDEiP08524.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL167W; YJL167W; YJL167W.
GeneIDi853272.
KEGGisce:YJL167W.

Organism-specific databases

CYGDiYJL167w.
SGDiS000003703. ERG20.

Phylogenomic databases

eggNOGiCOG0142.
GeneTreeiENSGT00530000064127.
HOGENOMiHOG000160912.
InParanoidiP08524.
KOiK00787.
OMAiCNGTHSK.
OrthoDBiEOG79GTJ1.

Enzyme and pathway databases

UniPathwayiUPA00259; UER00368.
UPA00260; UER00369.
BioCyciMetaCyc:MONOMER-655.
YEAST:MONOMER-655.
BRENDAi2.5.1.10. 984.
ReactomeiREACT_272228. Activation of gene expression by SREBF (SREBP).
REACT_283157. Cholesterol biosynthesis.

Miscellaneous databases

NextBioi973547.
PROiP08524.

Gene expression databases

GenevestigatoriP08524.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR000092. Polyprenyl_synt.
[Graphical view]
PfamiPF00348. polyprenyl_synt. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS00723. POLYPRENYL_SYNTHASE_1. 1 hit.
PS00444. POLYPRENYL_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Farnesyl diphosphate synthetase. Molecular cloning, sequence, and expression of an essential gene from Saccharomyces cerevisiae."
    Anderson M.S., Yarger J.G., Burck C.L., Poulter C.D.
    J. Biol. Chem. 264:19176-19184(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Nucleotide sequence of the gene encoding the 11-kDa subunit of the ubiquinol-cytochrome-c oxidoreductase in Saccharomyces cerevisiae."
    Maarse A.C., Grivell L.A.
    Eur. J. Biochem. 165:419-425(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 131-352.
  5. "Characterization of a lysine-to-glutamic acid mutation in a conservative sequence of farnesyl diphosphate synthase from Saccharomyces cerevisiae."
    Blanchard L., Karst F.
    Gene 125:185-189(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTANT GLU-197.
    Strain: LB25.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFPPS_YEAST
AccessioniPrimary (citable) accession number: P08524
Secondary accession number(s): D6VW20, P15495
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1991
Last modified: April 1, 2015
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.