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Protein

Farnesyl pyrophosphate synthase

Gene

ERG20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate.

Catalytic activityi

Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate.
Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate.

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Pathwayi: farnesyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Geranylgeranyl pyrophosphate synthase (BTS1), Farnesyl pyrophosphate synthase (ERG20)
This subpathway is part of the pathway farnesyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate, the pathway farnesyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Pathwayi: geranyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Geranylgeranyl pyrophosphate synthase (BTS1), Farnesyl pyrophosphate synthase (ERG20)
This subpathway is part of the pathway geranyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate, the pathway geranyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei52Isopentenyl diphosphateBy similarity1
Binding sitei55Isopentenyl diphosphateBy similarity1
Binding sitei93Isopentenyl diphosphateBy similarity1
Metal bindingi100Magnesium 1By similarity1
Metal bindingi100Magnesium 2By similarity1
Metal bindingi104Magnesium 1By similarity1
Metal bindingi104Magnesium 2By similarity1
Binding sitei109Dimethylallyl diphosphateBy similarity1
Binding sitei110Isopentenyl diphosphateBy similarity1
Binding sitei197Dimethylallyl diphosphateBy similarity1
Binding sitei198Dimethylallyl diphosphateBy similarity1
Binding sitei237Dimethylallyl diphosphateBy similarity1
Metal bindingi240Magnesium 3By similarity1
Binding sitei254Dimethylallyl diphosphateBy similarity1
Binding sitei263Dimethylallyl diphosphateBy similarity1

GO - Molecular functioni

  • dimethylallyltranstransferase activity Source: SGD
  • geranyltranstransferase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • ergosterol biosynthetic process Source: SGD
  • farnesyl diphosphate biosynthetic process Source: SGD
  • geranyl diphosphate biosynthetic process Source: UniProtKB-UniPathway
  • isoprenoid biosynthetic process Source: SGD

Keywordsi

Molecular functionTransferase
Biological processIsoprene biosynthesis, Lipid biosynthesis, Lipid metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-655.
YEAST:MONOMER-655.
BRENDAi2.5.1.10. 984.
ReactomeiR-SCE-191273. Cholesterol biosynthesis.
UniPathwayiUPA00259; UER00368.
UPA00260; UER00369.

Names & Taxonomyi

Protein namesi
Recommended name:
Farnesyl pyrophosphate synthase (EC:2.5.1.10)
Short name:
FPP synthase
Short name:
FPS
Alternative name(s):
(2E,6E)-farnesyl diphosphate synthase
Dimethylallyltranstransferase (EC:2.5.1.1)
Farnesyl diphosphate synthase
Geranyltranstransferase
Gene namesi
Name:ERG20
Synonyms:BOT3, FDS1, FPP1
Ordered Locus Names:YJL167W
ORF Names:J0525
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL167W.
SGDiS000003703. ERG20.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi197K → E in ERG20-2; 14-fold decrease in FPPS activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001239521 – 352Farnesyl pyrophosphate synthaseAdd BLAST352

Proteomic databases

MaxQBiP08524.
PRIDEiP08524.

Interactioni

Protein-protein interaction databases

BioGridi33592. 55 interactors.
DIPiDIP-1163N.
IntActiP08524. 13 interactors.
MINTiMINT-555012.
STRINGi4932.YJL167W.

Structurei

3D structure databases

ProteinModelPortaliP08524.
SMRiP08524.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FPP/GGPP synthase family.Curated

Phylogenomic databases

GeneTreeiENSGT00870000136449.
HOGENOMiHOG000160912.
InParanoidiP08524.
KOiK00787.
OMAiDIMDNKC.
OrthoDBiEOG092C35ZD.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiView protein in InterPro
IPR008949. Isoprenoid_synthase_dom.
IPR000092. Polyprenyl_synt.
IPR033749. Polyprenyl_synt_CS.
PfamiView protein in Pfam
PF00348. polyprenyl_synt. 1 hit.
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiView protein in PROSITE
PS00723. POLYPRENYL_SYNTHASE_1. 1 hit.
PS00444. POLYPRENYL_SYNTHASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P08524-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASEKEIRRE RFLNVFPKLV EELNASLLAY GMPKEACDWY AHSLNYNTPG
60 70 80 90 100
GKLNRGLSVV DTYAILSNKT VEQLGQEEYE KVAILGWCIE LLQAYFLVAD
110 120 130 140 150
DMMDKSITRR GQPCWYKVPE VGEIAINDAF MLEAAIYKLL KSHFRNEKYY
160 170 180 190 200
IDITELFHEV TFQTELGQLM DLITAPEDKV DLSKFSLKKH SFIVTFKTAY
210 220 230 240 250
YSFYLPVALA MYVAGITDEK DLKQARDVLI PLGEYFQIQD DYLDCFGTPE
260 270 280 290 300
QIGKIGTDIQ DNKCSWVINK ALELASAEQR KTLDENYGKK DSVAEAKCKK
310 320 330 340 350
IFNDLKIEQL YHEYEESIAK DLKAKISQVD ESRGFKADVL TAFLNKVYKR

SK
Length:352
Mass (Da):40,483
Last modified:November 1, 1991 - v2
Checksum:i79A357BB7BFCEDDA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05091 Genomic DNA. Translation: AAA34606.1.
Z49442 Genomic DNA. Translation: CAA89462.1.
X05550 Genomic DNA. Translation: CAA29064.1.
BK006943 Genomic DNA. Translation: DAA08636.1.
PIRiA34441.
RefSeqiNP_012368.1. NM_001181600.1.

Genome annotation databases

EnsemblFungiiYJL167W; YJL167W; YJL167W.
GeneIDi853272.
KEGGisce:YJL167W.

Similar proteinsi

Entry informationi

Entry nameiFPPS_YEAST
AccessioniPrimary (citable) accession number: P08524
Secondary accession number(s): D6VW20, P15495
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1991
Last modified: September 27, 2017
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names