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Protein

DNA-directed RNA polymerase II subunit RPB2

Gene

RPB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerases II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. During a transcription cycle, Pol II, general transcription factors and the Mediator complex assemble as the preinitiation complex (PIC) at the promoter. 11-15 base pairs of DNA surrounding the transcription start site are melted and the single-stranded DNA template strand of the promoter is positioned deeply within the central active site cleft of Pol II to form the open complex. After synthesis of about 30 bases of RNA, Pol II releases its contacts with the core promoter and the rest of the transcription machinery (promoter clearance) and enters the stage of transcription elongation in which it moves on the template as the transcript elongates. Pol II appears to oscillate between inactive and active conformations at each step of nucleotide addition. Pol II is composed of mobile elements that move relative to each other. The core element with the central large cleft comprises RPB3, RBP10, RPB11, RPB12 and regions of RPB1 and RPB2 forming the active center. The clamp element (portions of RPB1, RPB2 and RPB3) is connected to the core through a set of flexible switches and moves to open and close the cleft. The cleft is surrounded by jaws: an upper jaw formed by portions of RBP1, RPB2 and RPB9, and a lower jaw. The jaws are thought to grab the incoming DNA template. The fork loop 1 (RPB2) interacts with the RNA-DNA hybrid, possibly stabilizing it.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi837Magnesium; shared with RPB11
Metal bindingi1163Zinc1
Metal bindingi1166Zinc1
Metal bindingi1182Zinc1
Metal bindingi1185Zinc1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1163 – 1185C4-typeAdd BLAST23

GO - Molecular functioni

GO - Biological processi

  • transcription, RNA-templated Source: GOC
  • transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33668-MONOMER.
ReactomeiR-SCE-113418. Formation of the Early Elongation Complex.
R-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.
R-SCE-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SCE-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-SCE-72086. mRNA Capping.
R-SCE-72165. mRNA Splicing - Minor Pathway.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SCE-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase II subunit RPB2 (EC:2.7.7.6)
Short name:
RNA polymerase II subunit 2
Alternative name(s):
B150
DNA-directed RNA polymerase II 140 kDa polypeptide
Gene namesi
Name:RPB2
Synonyms:RPB150, RPO22
Ordered Locus Names:YOR151C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR151C.
SGDiS000005677. RPB2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic stress granule Source: SGD
  • DNA-directed RNA polymerase II, core complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000480911 – 1224DNA-directed RNA polymerase II subunit RPB2Add BLAST1224

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei919PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP08518.
PRIDEiP08518.

PTM databases

iPTMnetiP08518.

Interactioni

Subunit structurei

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RPB3P163705EBI-15767,EBI-15773
RPB5P204348EBI-15767,EBI-15781

Protein-protein interaction databases

BioGridi34547. 146 interactors.
DIPiDIP-14N.
IntActiP08518. 63 interactors.
MINTiMINT-654304.

Structurei

Secondary structure

11224
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi22 – 24Combined sources3
Helixi29 – 41Combined sources13
Helixi42 – 44Combined sources3
Helixi45 – 56Combined sources12
Helixi58 – 64Combined sources7
Beta strandi69 – 71Combined sources3
Beta strandi78 – 80Combined sources3
Beta strandi96 – 102Combined sources7
Beta strandi105 – 107Combined sources3
Beta strandi109 – 111Combined sources3
Helixi114 – 119Combined sources6
Beta strandi125 – 129Combined sources5
Beta strandi141 – 143Combined sources3
Beta strandi166 – 171Combined sources6
Beta strandi176 – 179Combined sources4
Helixi180 – 182Combined sources3
Helixi186 – 191Combined sources6
Beta strandi202 – 205Combined sources4
Beta strandi208 – 212Combined sources5
Beta strandi214 – 218Combined sources5
Beta strandi224 – 227Combined sources4
Beta strandi232 – 241Combined sources10
Beta strandi245 – 247Combined sources3
Beta strandi253 – 260Combined sources8
Beta strandi264 – 266Combined sources3
Beta strandi269 – 272Combined sources4
Beta strandi277 – 281Combined sources5
Helixi282 – 288Combined sources7
Helixi294 – 301Combined sources8
Helixi308 – 320Combined sources13
Turni321 – 323Combined sources3
Helixi327 – 336Combined sources10
Beta strandi337 – 339Combined sources3
Helixi342 – 344Combined sources3
Helixi345 – 358Combined sources14
Beta strandi359 – 361Combined sources3
Turni362 – 364Combined sources3
Beta strandi367 – 369Combined sources3
Helixi371 – 389Combined sources19
Beta strandi390 – 393Combined sources4
Helixi401 – 403Combined sources3
Beta strandi404 – 407Combined sources4
Helixi409 – 433Combined sources25
Turni434 – 436Combined sources3
Helixi444 – 447Combined sources4
Helixi451 – 463Combined sources13
Beta strandi467 – 469Combined sources3
Helixi474 – 476Combined sources3
Beta strandi477 – 479Combined sources3
Beta strandi480 – 482Combined sources3
Helixi488 – 495Combined sources8
Beta strandi497 – 499Combined sources3
Turni510 – 512Combined sources3
Helixi516 – 518Combined sources3
Turni519 – 521Combined sources3
Helixi530 – 532Combined sources3
Turni533 – 535Combined sources3
Beta strandi536 – 539Combined sources4
Helixi552 – 560Combined sources9
Beta strandi563 – 565Combined sources3
Helixi566 – 568Combined sources3
Helixi571 – 573Combined sources3
Beta strandi574 – 576Combined sources3
Beta strandi578 – 582Combined sources5
Beta strandi585 – 591Combined sources7
Helixi593 – 606Combined sources14
Beta strandi607 – 609Combined sources3
Beta strandi614 – 618Combined sources5
Turni619 – 622Combined sources4
Beta strandi623 – 627Combined sources5
Beta strandi633 – 641Combined sources9
Beta strandi646 – 649Combined sources4
Beta strandi650 – 652Combined sources3
Helixi655 – 666Combined sources12
Helixi681 – 686Combined sources6
Beta strandi689 – 694Combined sources6
Helixi695 – 698Combined sources4
Beta strandi703 – 706Combined sources4
Helixi707 – 710Combined sources4
Beta strandi725 – 727Combined sources3
Beta strandi739 – 741Combined sources3
Helixi745 – 748Combined sources4
Helixi753 – 755Combined sources3
Beta strandi756 – 758Combined sources3
Helixi759 – 761Combined sources3
Helixi764 – 773Combined sources10
Helixi774 – 776Combined sources3
Beta strandi779 – 781Combined sources3
Turni783 – 787Combined sources5
Beta strandi791 – 798Combined sources8
Beta strandi803 – 805Combined sources3
Helixi809 – 812Combined sources4
Turni813 – 816Combined sources4
Beta strandi821 – 827Combined sources7
Beta strandi829 – 832Combined sources4
Turni833 – 837Combined sources5
Beta strandi838 – 842Combined sources5
Helixi843 – 847Combined sources5
Turni848 – 851Combined sources4
Beta strandi853 – 861Combined sources9
Beta strandi866 – 869Combined sources4
Beta strandi873 – 875Combined sources3
Beta strandi880 – 882Combined sources3
Beta strandi886 – 888Combined sources3
Helixi890 – 892Combined sources3
Beta strandi895 – 899Combined sources5
Beta strandi904 – 906Combined sources3
Beta strandi910 – 912Combined sources3
Beta strandi914 – 916Combined sources3
Beta strandi935 – 940Combined sources6
Beta strandi947 – 956Combined sources10
Beta strandi958 – 960Combined sources3
Beta strandi962 – 972Combined sources11
Beta strandi979 – 981Combined sources3
Beta strandi983 – 985Combined sources3
Beta strandi987 – 994Combined sources8
Turni996 – 998Combined sources3
Beta strandi1009 – 1012Combined sources4
Helixi1014 – 1016Combined sources3
Turni1018 – 1021Combined sources4
Helixi1023 – 1038Combined sources16
Beta strandi1046 – 1049Combined sources4
Helixi1052 – 1060Combined sources9
Turni1061 – 1063Combined sources3
Beta strandi1068 – 1070Combined sources3
Beta strandi1071 – 1073Combined sources3
Turni1075 – 1077Combined sources3
Beta strandi1080 – 1084Combined sources5
Beta strandi1085 – 1097Combined sources13
Helixi1099 – 1101Combined sources3
Beta strandi1104 – 1106Combined sources3
Turni1113 – 1115Combined sources3
Helixi1122 – 1124Combined sources3
Beta strandi1128 – 1130Combined sources3
Helixi1132 – 1141Combined sources10
Helixi1144 – 1151Combined sources8
Helixi1152 – 1156Combined sources5
Beta strandi1159 – 1166Combined sources8
Beta strandi1172 – 1174Combined sources3
Turni1175 – 1178Combined sources4
Beta strandi1179 – 1182Combined sources4
Turni1183 – 1186Combined sources4
Beta strandi1187 – 1195Combined sources9
Helixi1198 – 1209Combined sources12
Beta strandi1215 – 1218Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I3QX-ray3.10B1-1224[»]
1I50X-ray2.80B1-1224[»]
1I6HX-ray3.30B1-1224[»]
1K83X-ray2.80B1-1224[»]
1NIKX-ray4.10B1-1224[»]
1NT9X-ray4.20B1-1224[»]
1PQVX-ray3.80B1-1224[»]
1R5UX-ray4.50B1-1224[»]
1R9SX-ray4.25B1-1224[»]
1R9TX-ray3.50B1-1224[»]
1SFOX-ray3.61B1-1224[»]
1TWAX-ray3.20B1-1224[»]
1TWCX-ray3.00B1-1224[»]
1TWFX-ray2.30B1-1224[»]
1TWGX-ray3.30B1-1224[»]
1TWHX-ray3.40B1-1224[»]
1WCMX-ray3.80B1-1224[»]
1Y1VX-ray3.80B1-1224[»]
1Y1WX-ray4.00B1-1224[»]
1Y1YX-ray4.00B1-1224[»]
1Y77X-ray4.50B1-1224[»]
2B63X-ray3.80B1-1224[»]
2B8KX-ray4.15B1-1224[»]
2E2HX-ray3.95B1-1224[»]
2E2IX-ray3.41B1-1224[»]
2E2JX-ray3.50B1-1224[»]
2JA5X-ray3.80B1-1224[»]
2JA6X-ray4.00B1-1224[»]
2JA7X-ray3.80B/N1-1224[»]
2JA8X-ray3.80B1-1224[»]
2NVQX-ray2.90B1-1224[»]
2NVTX-ray3.36B1-1224[»]
2NVXX-ray3.60B1-1224[»]
2NVYX-ray3.40B1-1224[»]
2NVZX-ray4.30B1-1224[»]
2R7ZX-ray3.80B1-1224[»]
2R92X-ray3.80B1-1224[»]
2R93X-ray4.00B1-1224[»]
2VUMX-ray3.40B1-1224[»]
2YU9X-ray3.40B1-1224[»]
3CQZX-ray2.80B1-1223[»]
3FKIX-ray3.88B1-1224[»]
3GTGX-ray3.78B1-1224[»]
3GTJX-ray3.42B1-1224[»]
3GTKX-ray3.80B1-1224[»]
3GTLX-ray3.38B1-1224[»]
3GTMX-ray3.80B1-1224[»]
3GTOX-ray4.00B1-1224[»]
3GTPX-ray3.90B1-1224[»]
3GTQX-ray3.80B1-1224[»]
3H3VX-ray4.00C1-1224[»]
3HOUX-ray3.20B/N1-1224[»]
3HOVX-ray3.50B1-1224[»]
3HOWX-ray3.60B1-1224[»]
3HOXX-ray3.65B1-1224[»]
3HOYX-ray3.40B1-1224[»]
3HOZX-ray3.65B1-1224[»]
3I4MX-ray3.70B1-1224[»]
3I4NX-ray3.90B1-1224[»]
3J0Kelectron microscopy36.00B1-1224[»]
3J1Nelectron microscopy16.00B1-1224[»]
3K1FX-ray4.30B1-1224[»]
3K7AX-ray3.80B1-1224[»]
3M3YX-ray3.18B1-1224[»]
3M4OX-ray3.57B1-1224[»]
3PO2X-ray3.30B1-1224[»]
3PO3X-ray3.30B1-1224[»]
3QT1X-ray4.30B1-1224[»]
3RZDX-ray3.30B1-1224[»]
3RZOX-ray3.00B1-1224[»]
3S14X-ray2.85B1-1224[»]
3S15X-ray3.30B1-1224[»]
3S16X-ray3.24B1-1224[»]
3S17X-ray3.20B1-1224[»]
3S1MX-ray3.13B1-1224[»]
3S1NX-ray3.10B1-1224[»]
3S1QX-ray3.30B1-1224[»]
3S1RX-ray3.20B1-1224[»]
3S2DX-ray3.20B1-1224[»]
3S2HX-ray3.30B1-1224[»]
4A3BX-ray3.50B1-1224[»]
4A3CX-ray3.50B1-1224[»]
4A3DX-ray3.40B1-1224[»]
4A3EX-ray3.40B1-1224[»]
4A3FX-ray3.50B1-1224[»]
4A3GX-ray3.50B1-1224[»]
4A3IX-ray3.80B1-1224[»]
4A3JX-ray3.70B1-1224[»]
4A3KX-ray3.50B1-1224[»]
4A3LX-ray3.50B1-1224[»]
4A3MX-ray3.90B1-1224[»]
4A93X-ray3.40B1-1224[»]
4BBRX-ray3.40B1-1224[»]
4BBSX-ray3.60B1-1224[»]
4BXXX-ray3.28B1-1224[»]
4BXZX-ray4.80B1-1224[»]
4BY1X-ray3.60B1-1224[»]
4BY7X-ray3.15B1-1224[»]
4V1Melectron microscopy6.60B1-1224[»]
4V1Nelectron microscopy7.80B1-1224[»]
4V1Oelectron microscopy9.70B1-1224[»]
4X67X-ray4.10B1-1224[»]
4X6AX-ray3.96B1-1224[»]
4Y52X-ray3.50B1-1224[»]
4Y7NX-ray3.30B1-1224[»]
5C3EX-ray3.70B1-1224[»]
5C44X-ray3.95B1-1224[»]
5C4AX-ray4.20B1-1224[»]
5C4JX-ray4.00B1-1224[»]
5C4XX-ray4.00B1-1224[»]
5FMFelectron microscopy6.00B1-1224[»]
5FYWelectron microscopy4.35B1-1224[»]
5FZ5electron microscopy8.80B1-1224[»]
5IP7X-ray3.52B2-1224[»]
5IP9X-ray3.90B2-1224[»]
5SVAelectron microscopy15.30B1-1224[»]
ProteinModelPortaliP08518.
SMRiP08518.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08518.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni467 – 478Fork loop 1Add BLAST12

Sequence similaritiesi

Belongs to the RNA polymerase beta chain family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1163 – 1185C4-typeAdd BLAST23

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00860000133929.
HOGENOMiHOG000222962.
InParanoidiP08518.
KOiK03010.
OMAiRTQPHFE.
OrthoDBiEOG092C06IY.

Family and domain databases

CDDicd00653. RNA_pol_B_RPB2. 1 hit.
Gene3Di2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
InterProiIPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007646. RNA_pol_Rpb2_4.
IPR007647. RNA_pol_Rpb2_5.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 2 hits.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF04566. RNA_pol_Rpb2_4. 1 hit.
PF04567. RNA_pol_Rpb2_5. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08518-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDLANSEKY YDEDPYGFED ESAPITAEDS WAVISAFFRE KGLVSQQLDS
60 70 80 90 100
FNQFVDYTLQ DIICEDSTLI LEQLAQHTTE SDNISRKYEI SFGKIYVTKP
110 120 130 140 150
MVNESDGVTH ALYPQEARLR NLTYSSGLFV DVKKRTYEAI DVPGRELKYE
160 170 180 190 200
LIAEESEDDS ESGKVFIGRL PIMLRSKNCY LSEATESDLY KLKECPFDMG
210 220 230 240 250
GYFIINGSEK VLIAQERSAG NIVQVFKKAA PSPISHVAEI RSALEKGSRF
260 270 280 290 300
ISTLQVKLYG REGSSARTIK ATLPYIKQDI PIVIIFRALG IIPDGEILEH
310 320 330 340 350
ICYDVNDWQM LEMLKPCVED GFVIQDRETA LDFIGRRGTA LGIKKEKRIQ
360 370 380 390 400
YAKDILQKEF LPHITQLEGF ESRKAFFLGY MINRLLLCAL DRKDQDDRDH
410 420 430 440 450
FGKKRLDLAG PLLAQLFKTL FKKLTKDIFR YMQRTVEEAH DFNMKLAINA
460 470 480 490 500
KTITSGLKYA LATGNWGEQK KAMSSRAGVS QVLNRYTYSS TLSHLRRTNT
510 520 530 540 550
PIGRDGKLAK PRQLHNTHWG LVCPAETPEG QACGLVKNLS LMSCISVGTD
560 570 580 590 600
PMPIITFLSE WGMEPLEDYV PHQSPDATRV FVNGVWHGVH RNPARLMETL
610 620 630 640 650
RTLRRKGDIN PEVSMIRDIR EKELKIFTDA GRVYRPLFIV EDDESLGHKE
660 670 680 690 700
LKVRKGHIAK LMATEYQDIE GGFEDVEEYT WSSLLNEGLV EYIDAEEEES
710 720 730 740 750
ILIAMQPEDL EPAEANEEND LDVDPAKRIR VSHHATTFTH CEIHPSMILG
760 770 780 790 800
VAASIIPFPD HNQSPRNTYQ SAMGKQAMGV FLTNYNVRMD TMANILYYPQ
810 820 830 840 850
KPLGTTRAME YLKFRELPAG QNAIVAIACY SGYNQEDSMI MNQSSIDRGL
860 870 880 890 900
FRSLFFRSYM DQEKKYGMSI TETFEKPQRT NTLRMKHGTY DKLDDDGLIA
910 920 930 940 950
PGVRVSGEDV IIGKTTPISP DEEELGQRTA YHSKRDASTP LRSTENGIVD
960 970 980 990 1000
QVLVTTNQDG LKFVKVRVRT TKIPQIGDKF ASRHGQKGTI GITYRREDMP
1010 1020 1030 1040 1050
FTAEGIVPDL IINPHAIPSR MTVAHLIECL LSKVAALSGN EGDASPFTDI
1060 1070 1080 1090 1100
TVEGISKLLR EHGYQSRGFE VMYNGHTGKK LMAQIFFGPT YYQRLRHMVD
1110 1120 1130 1140 1150
DKIHARARGP MQVLTRQPVE GRSRDGGLRF GEMERDCMIA HGAASFLKER
1160 1170 1180 1190 1200
LMEASDAFRV HICGICGLMT VIAKLNHNQF ECKGCDNKID IYQIHIPYAA
1210 1220
KLLFQELMAM NITPRLYTDR SRDF
Length:1,224
Mass (Da):138,751
Last modified:November 1, 1997 - v2
Checksum:iBABD03212C0A583E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1003 – 1006AEGI → RRRY in AAA68096 (PubMed:3547406).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15693 Genomic DNA. Translation: AAA68096.1.
U55020 Genomic DNA. Translation: AAC49637.1.
Z75059 Genomic DNA. Translation: CAA99357.1.
AF527884 Genomic DNA. Translation: AAP57849.1.
BK006948 Genomic DNA. Translation: DAA10924.1.
PIRiA25884.
RefSeqiNP_014794.3. NM_001183570.3.

Genome annotation databases

EnsemblFungiiYOR151C; YOR151C; YOR151C.
GeneIDi854322.
KEGGisce:YOR151C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15693 Genomic DNA. Translation: AAA68096.1.
U55020 Genomic DNA. Translation: AAC49637.1.
Z75059 Genomic DNA. Translation: CAA99357.1.
AF527884 Genomic DNA. Translation: AAP57849.1.
BK006948 Genomic DNA. Translation: DAA10924.1.
PIRiA25884.
RefSeqiNP_014794.3. NM_001183570.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I3QX-ray3.10B1-1224[»]
1I50X-ray2.80B1-1224[»]
1I6HX-ray3.30B1-1224[»]
1K83X-ray2.80B1-1224[»]
1NIKX-ray4.10B1-1224[»]
1NT9X-ray4.20B1-1224[»]
1PQVX-ray3.80B1-1224[»]
1R5UX-ray4.50B1-1224[»]
1R9SX-ray4.25B1-1224[»]
1R9TX-ray3.50B1-1224[»]
1SFOX-ray3.61B1-1224[»]
1TWAX-ray3.20B1-1224[»]
1TWCX-ray3.00B1-1224[»]
1TWFX-ray2.30B1-1224[»]
1TWGX-ray3.30B1-1224[»]
1TWHX-ray3.40B1-1224[»]
1WCMX-ray3.80B1-1224[»]
1Y1VX-ray3.80B1-1224[»]
1Y1WX-ray4.00B1-1224[»]
1Y1YX-ray4.00B1-1224[»]
1Y77X-ray4.50B1-1224[»]
2B63X-ray3.80B1-1224[»]
2B8KX-ray4.15B1-1224[»]
2E2HX-ray3.95B1-1224[»]
2E2IX-ray3.41B1-1224[»]
2E2JX-ray3.50B1-1224[»]
2JA5X-ray3.80B1-1224[»]
2JA6X-ray4.00B1-1224[»]
2JA7X-ray3.80B/N1-1224[»]
2JA8X-ray3.80B1-1224[»]
2NVQX-ray2.90B1-1224[»]
2NVTX-ray3.36B1-1224[»]
2NVXX-ray3.60B1-1224[»]
2NVYX-ray3.40B1-1224[»]
2NVZX-ray4.30B1-1224[»]
2R7ZX-ray3.80B1-1224[»]
2R92X-ray3.80B1-1224[»]
2R93X-ray4.00B1-1224[»]
2VUMX-ray3.40B1-1224[»]
2YU9X-ray3.40B1-1224[»]
3CQZX-ray2.80B1-1223[»]
3FKIX-ray3.88B1-1224[»]
3GTGX-ray3.78B1-1224[»]
3GTJX-ray3.42B1-1224[»]
3GTKX-ray3.80B1-1224[»]
3GTLX-ray3.38B1-1224[»]
3GTMX-ray3.80B1-1224[»]
3GTOX-ray4.00B1-1224[»]
3GTPX-ray3.90B1-1224[»]
3GTQX-ray3.80B1-1224[»]
3H3VX-ray4.00C1-1224[»]
3HOUX-ray3.20B/N1-1224[»]
3HOVX-ray3.50B1-1224[»]
3HOWX-ray3.60B1-1224[»]
3HOXX-ray3.65B1-1224[»]
3HOYX-ray3.40B1-1224[»]
3HOZX-ray3.65B1-1224[»]
3I4MX-ray3.70B1-1224[»]
3I4NX-ray3.90B1-1224[»]
3J0Kelectron microscopy36.00B1-1224[»]
3J1Nelectron microscopy16.00B1-1224[»]
3K1FX-ray4.30B1-1224[»]
3K7AX-ray3.80B1-1224[»]
3M3YX-ray3.18B1-1224[»]
3M4OX-ray3.57B1-1224[»]
3PO2X-ray3.30B1-1224[»]
3PO3X-ray3.30B1-1224[»]
3QT1X-ray4.30B1-1224[»]
3RZDX-ray3.30B1-1224[»]
3RZOX-ray3.00B1-1224[»]
3S14X-ray2.85B1-1224[»]
3S15X-ray3.30B1-1224[»]
3S16X-ray3.24B1-1224[»]
3S17X-ray3.20B1-1224[»]
3S1MX-ray3.13B1-1224[»]
3S1NX-ray3.10B1-1224[»]
3S1QX-ray3.30B1-1224[»]
3S1RX-ray3.20B1-1224[»]
3S2DX-ray3.20B1-1224[»]
3S2HX-ray3.30B1-1224[»]
4A3BX-ray3.50B1-1224[»]
4A3CX-ray3.50B1-1224[»]
4A3DX-ray3.40B1-1224[»]
4A3EX-ray3.40B1-1224[»]
4A3FX-ray3.50B1-1224[»]
4A3GX-ray3.50B1-1224[»]
4A3IX-ray3.80B1-1224[»]
4A3JX-ray3.70B1-1224[»]
4A3KX-ray3.50B1-1224[»]
4A3LX-ray3.50B1-1224[»]
4A3MX-ray3.90B1-1224[»]
4A93X-ray3.40B1-1224[»]
4BBRX-ray3.40B1-1224[»]
4BBSX-ray3.60B1-1224[»]
4BXXX-ray3.28B1-1224[»]
4BXZX-ray4.80B1-1224[»]
4BY1X-ray3.60B1-1224[»]
4BY7X-ray3.15B1-1224[»]
4V1Melectron microscopy6.60B1-1224[»]
4V1Nelectron microscopy7.80B1-1224[»]
4V1Oelectron microscopy9.70B1-1224[»]
4X67X-ray4.10B1-1224[»]
4X6AX-ray3.96B1-1224[»]
4Y52X-ray3.50B1-1224[»]
4Y7NX-ray3.30B1-1224[»]
5C3EX-ray3.70B1-1224[»]
5C44X-ray3.95B1-1224[»]
5C4AX-ray4.20B1-1224[»]
5C4JX-ray4.00B1-1224[»]
5C4XX-ray4.00B1-1224[»]
5FMFelectron microscopy6.00B1-1224[»]
5FYWelectron microscopy4.35B1-1224[»]
5FZ5electron microscopy8.80B1-1224[»]
5IP7X-ray3.52B2-1224[»]
5IP9X-ray3.90B2-1224[»]
5SVAelectron microscopy15.30B1-1224[»]
ProteinModelPortaliP08518.
SMRiP08518.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34547. 146 interactors.
DIPiDIP-14N.
IntActiP08518. 63 interactors.
MINTiMINT-654304.

PTM databases

iPTMnetiP08518.

Proteomic databases

MaxQBiP08518.
PRIDEiP08518.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR151C; YOR151C; YOR151C.
GeneIDi854322.
KEGGisce:YOR151C.

Organism-specific databases

EuPathDBiFungiDB:YOR151C.
SGDiS000005677. RPB2.

Phylogenomic databases

GeneTreeiENSGT00860000133929.
HOGENOMiHOG000222962.
InParanoidiP08518.
KOiK03010.
OMAiRTQPHFE.
OrthoDBiEOG092C06IY.

Enzyme and pathway databases

BioCyciYEAST:G3O-33668-MONOMER.
ReactomeiR-SCE-113418. Formation of the Early Elongation Complex.
R-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.
R-SCE-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SCE-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-SCE-72086. mRNA Capping.
R-SCE-72165. mRNA Splicing - Minor Pathway.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SCE-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

EvolutionaryTraceiP08518.
PROiP08518.

Family and domain databases

CDDicd00653. RNA_pol_B_RPB2. 1 hit.
Gene3Di2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
InterProiIPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007646. RNA_pol_Rpb2_4.
IPR007647. RNA_pol_Rpb2_5.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 2 hits.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF04566. RNA_pol_Rpb2_4. 1 hit.
PF04567. RNA_pol_Rpb2_5. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPB2_YEAST
AccessioniPrimary (citable) accession number: P08518
Secondary accession number(s): D6W2K8, Q12738, Q7Z9Y0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 182 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits.
Present with 18700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.