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Protein

Cytochrome P450 4A10

Gene

Cyp4a10

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

A long-chain fatty acid + NADPH + O2 = an omega-hydroxy-long-chain fatty acid + NADP+ + H2O.By similarity
Octane + 2 reduced rubredoxin + O2 + 2 H+ = 1-octanol + 2 oxidized rubredoxin + H2O.

Cofactori

heme2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei320Heme (covalent; via 1 link)2 Publications1
Metal bindingi456Iron (heme axial ligand)2 Publications1

GO - Molecular functioni

  • alkane 1-monooxygenase activity Source: RGD
  • arachidonic acid monooxygenase activity Source: RGD
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro

GO - Biological processi

  • arachidonic acid metabolic process Source: RGD
  • icosanoid biosynthetic process Source: RGD
  • kidney development Source: RGD
  • lauric acid metabolic process Source: RGD
  • linoleic acid metabolic process Source: RGD
  • placenta development Source: RGD

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-RNO-211935 Fatty acids
R-RNO-211958 Miscellaneous substrates
R-RNO-211979 Eicosanoids
R-RNO-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
R-RNO-2142816 Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE)

Chemistry databases

SwissLipidsiSLP:000000747

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 4A10
Alternative name(s):
CYPIVA10
Cytochrome P450-LA-omega 1
Cytochrome P452
Lauric acid omega-hydroxylase
Long-chain fatty acid omega-monooxygenase (EC:1.14.13.205)
Gene namesi
Name:Cyp4a10
Synonyms:Cyp4a-1, Cyp4a1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi68945 Cyp4a1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi320E → A: Loss of heme binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000518161 – 509Cytochrome P450 4A10Add BLAST509

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei439PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP08516
PRIDEiP08516

PTM databases

iPTMnetiP08516
PhosphoSitePlusiP08516

Expressioni

Inductioni

By clofibrate.

Gene expression databases

BgeeiENSRNOG00000009597
ExpressionAtlasiP08516 baseline and differential
GenevisibleiP08516 RN

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000041025

Structurei

3D structure databases

ProteinModelPortaliP08516
SMRiP08516
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0157 Eukaryota
COG2124 LUCA
GeneTreeiENSGT00900000140829
HOGENOMiHOG000233833
HOVERGENiHBG000182
InParanoidiP08516
KOiK07425
OMAiLAMTTVY
OrthoDBiEOG091G06KN
PhylomeDBiP08516

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

P08516-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVSALSSTR FTGSISGFLQ VASVLGLLLL LVKAVQFYLQ RQWLLKAFQQ
60 70 80 90 100
FPSPPFHWFF GHKQFQGDKE LQQIMTCVEN FPSAFPRWFW GSKAYLIVYD
110 120 130 140 150
PDYMKVILGR SDPKANGVYR LLAPWIGYGL LLLNGQPWFQ HRRMLTPAFH
160 170 180 190 200
YDILKPYVKN MADSIRLMLD KWEQLAGQDS SIEIFQHISL MTLDTVMKCA
210 220 230 240 250
FSHNGSVQVD GNYKSYIQAI GNLNDLFHSR VRNIFHQNDT IYNFSSNGHL
260 270 280 290 300
FNRACQLAHD HTDGVIKLRK DQLQNAGELE KVKKKRRLDF LDILLLARME
310 320 330 340 350
NGDSLSDKDL RAEVDTFMFE GHDTTASGVS WIFYALATHP EHQQRCREEV
360 370 380 390 400
QSVLGDGSSI TWDHLDQIPY TTMCIKEALR LYPPVPGIVR ELSTSVTFPD
410 420 430 440 450
GRSLPKGIQV TLSIYGLHHN PKVWPNPEVF DPSRFAPDSP RHSHSFLPFS
460 470 480 490 500
GGARNCIGKQ FAMSEMKVIV ALTLLRFELL PDPTKVPIPL PRLVLKSKNG

IYLYLKKLH
Length:509
Mass (Da):58,215
Last modified:May 1, 2007 - v2
Checksum:iE622FAB351BC40AF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti341E → K in AAA41061 (PubMed:3027069).Curated1
Sequence conflicti341E → K in CAA30245 (PubMed:3410047).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14972 mRNA Translation: AAA41061.1
X07259 mRNA Translation: CAA30245.1
M57718 Genomic DNA Translation: AAA41038.1
BC089761 mRNA Translation: AAH89761.1
PIRiS01336 O4RTLO
RefSeqiNP_787031.1, NM_175837.1
XP_006238774.1, XM_006238712.3
UniGeneiRn.92401

Genome annotation databases

EnsembliENSRNOT00000012888; ENSRNOP00000012888; ENSRNOG00000009597
GeneIDi50549
KEGGirno:50549

Similar proteinsi

Entry informationi

Entry nameiCP4AA_RAT
AccessioniPrimary (citable) accession number: P08516
Secondary accession number(s): Q5EBD8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: May 1, 2007
Last modified: May 23, 2018
This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health