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Protein

Cytochrome P450 4A10

Gene

Cyp4a10

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

A long-chain fatty acid + NADPH + O2 = an omega-hydroxy-long-chain fatty acid + NADP+ + H2O.By similarity
Octane + 2 reduced rubredoxin + O2 + 2 H+ = 1-octanol + 2 oxidized rubredoxin + H2O.

Cofactori

heme2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei320 – 3201Heme (covalent; via 1 link)2 Publications
Metal bindingi456 – 4561Iron (heme axial ligand)2 Publications

GO - Molecular functioni

  • alkane 1-monooxygenase activity Source: RGD
  • arachidonic acid monooxygenase activity Source: RGD
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro

GO - Biological processi

  • arachidonic acid metabolic process Source: RGD
  • icosanoid biosynthetic process Source: RGD
  • kidney development Source: RGD
  • lauric acid metabolic process Source: RGD
  • linoleic acid metabolic process Source: RGD
  • placenta development Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-RNO-211935. Fatty acids.
R-RNO-2142816. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).

Chemistry

SwissLipidsiSLP:000000747.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 4A10
Alternative name(s):
CYPIVA10
Cytochrome P450-LA-omega 1
Cytochrome P452
Lauric acid omega-hydroxylase
Long-chain fatty acid omega-monooxygenase (EC:1.14.13.205)
Cleaved into the following chain:
Gene namesi
Name:Cyp4a10
Synonyms:Cyp4a-1, Cyp4a1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi68945. Cyp4a1.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi320 – 3201E → A: Loss of heme binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 509509Cytochrome P450 4A10PRO_0000051816Add
BLAST
Initiator methionineiRemoved; alternateBy similarity
Chaini2 – 509508Cytochrome P450 4A10, N-terminally processedPRO_0000436693Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei439 – 4391PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP08516.
PRIDEiP08516.

PTM databases

iPTMnetiP08516.

Expressioni

Inductioni

By clofibrate.

Gene expression databases

ExpressionAtlasiP08516. baseline and differential.
GenevisibleiP08516. RN.

Interactioni

Protein-protein interaction databases

MINTiMINT-4576047.
STRINGi10116.ENSRNOP00000041025.

Structurei

3D structure databases

ProteinModelPortaliP08516.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118816.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiP08516.
KOiK07425.
PhylomeDBiP08516.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08516-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVSALSSTR FTGSISGFLQ VASVLGLLLL LVKAVQFYLQ RQWLLKAFQQ
60 70 80 90 100
FPSPPFHWFF GHKQFQGDKE LQQIMTCVEN FPSAFPRWFW GSKAYLIVYD
110 120 130 140 150
PDYMKVILGR SDPKANGVYR LLAPWIGYGL LLLNGQPWFQ HRRMLTPAFH
160 170 180 190 200
YDILKPYVKN MADSIRLMLD KWEQLAGQDS SIEIFQHISL MTLDTVMKCA
210 220 230 240 250
FSHNGSVQVD GNYKSYIQAI GNLNDLFHSR VRNIFHQNDT IYNFSSNGHL
260 270 280 290 300
FNRACQLAHD HTDGVIKLRK DQLQNAGELE KVKKKRRLDF LDILLLARME
310 320 330 340 350
NGDSLSDKDL RAEVDTFMFE GHDTTASGVS WIFYALATHP EHQQRCREEV
360 370 380 390 400
QSVLGDGSSI TWDHLDQIPY TTMCIKEALR LYPPVPGIVR ELSTSVTFPD
410 420 430 440 450
GRSLPKGIQV TLSIYGLHHN PKVWPNPEVF DPSRFAPDSP RHSHSFLPFS
460 470 480 490 500
GGARNCIGKQ FAMSEMKVIV ALTLLRFELL PDPTKVPIPL PRLVLKSKNG

IYLYLKKLH
Length:509
Mass (Da):58,215
Last modified:May 1, 2007 - v2
Checksum:iE622FAB351BC40AF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti341 – 3411E → K in AAA41061 (PubMed:3027069).Curated
Sequence conflicti341 – 3411E → K in CAA30245 (PubMed:3410047).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14972 mRNA. Translation: AAA41061.1.
X07259 mRNA. Translation: CAA30245.1.
M57718 Genomic DNA. Translation: AAA41038.1.
BC089761 mRNA. Translation: AAH89761.1.
PIRiS01336. O4RTLO.
RefSeqiNP_787031.1. NM_175837.1.
XP_006238774.1. XM_006238712.2.
UniGeneiRn.92401.

Genome annotation databases

EnsembliENSRNOT00000012888; ENSRNOP00000012888; ENSRNOG00000009597.
GeneIDi50549.
KEGGirno:50549.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14972 mRNA. Translation: AAA41061.1.
X07259 mRNA. Translation: CAA30245.1.
M57718 Genomic DNA. Translation: AAA41038.1.
BC089761 mRNA. Translation: AAH89761.1.
PIRiS01336. O4RTLO.
RefSeqiNP_787031.1. NM_175837.1.
XP_006238774.1. XM_006238712.2.
UniGeneiRn.92401.

3D structure databases

ProteinModelPortaliP08516.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4576047.
STRINGi10116.ENSRNOP00000041025.

Chemistry

SwissLipidsiSLP:000000747.

PTM databases

iPTMnetiP08516.

Proteomic databases

PaxDbiP08516.
PRIDEiP08516.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012888; ENSRNOP00000012888; ENSRNOG00000009597.
GeneIDi50549.
KEGGirno:50549.

Organism-specific databases

CTDi50549.
RGDi68945. Cyp4a1.

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118816.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiP08516.
KOiK07425.
PhylomeDBiP08516.

Enzyme and pathway databases

ReactomeiR-RNO-211935. Fatty acids.
R-RNO-2142816. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).

Miscellaneous databases

PROiP08516.

Gene expression databases

ExpressionAtlasiP08516. baseline and differential.
GenevisibleiP08516. RN.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, complementary DNA sequence, and regulation of rat hepatic lauric acid omega-hydroxylase (cytochrome P-450LA omega). Identification of a new cytochrome P-450 gene family."
    Hardwick J.P., Song B.-J., Huberman E., Gonzalez F.J.
    J. Biol. Chem. 262:801-810(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Differential splicing in the 3' non-coding region of rat cytochrome P-452 (P450 IVA1) mRNA."
    Earnshaw D., Dale J.W., Goldfarb P.S., Gibson G.G.
    FEBS Lett. 236:357-361(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Liver.
  3. "The rat clofibrate-inducible CYP4A gene subfamily. I. Complete intron and exon sequence of the CYP4A1 and CYP4A2 genes, unique exon organization, and identification of a conserved 19-bp upstream element."
    Kimura S., Hanioka N., Matsunaga E., Gonzalez F.J.
    DNA 8:503-516(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  5. "Characterization of a cytochrome P450 from di(2-ethylhexyl) phthalate-treated rats which hydroxylates fatty acids."
    Okita R.T., Okita J.R.
    Arch. Biochem. Biophys. 294:475-481(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-22.
  6. "Covalently linked heme in cytochrome P4504A fatty acid hydroxylases."
    Hoch U., Ortiz de Montellano P.R.
    J. Biol. Chem. 276:11339-11346(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: COVALENT HEME ATTACHMENT.
  7. "Autocatalytic mechanism and consequences of covalent heme attachment in the cytochrome P4504A family."
    LeBrun L.A., Hoch U., Ortiz de Montellano P.R.
    J. Biol. Chem. 277:12755-12761(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: COVALENT HEME ATTACHMENT, MUTAGENESIS OF GLU-320.

Entry informationi

Entry nameiCP4AA_RAT
AccessioniPrimary (citable) accession number: P08516
Secondary accession number(s): Q5EBD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: May 1, 2007
Last modified: July 6, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.