Reviewed, UniProtKB/Swiss-Prot P08516 (CP4AA_RAT)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 4A10
    EC=1.14.15.3
Alternative name(s):
    CYPIVA10
    Lauric acid omega-hydroxylase
    P450-LA-omega 1
    P452

Gene names

Name:	Cyp4a10
Synonyms:	Cyp4a-1, Cyp4a1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	Octane + reduced rubredoxin + O(2) = 1-octanol + oxidized rubredoxin + H(2)O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	By clofibrate.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alkane 1-monooxygenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 509	509	Cytochrome P450 4A10		PRO_0000051816
Sites
	Metal binding	456	1	Iron (heme axial ligand)
	Binding site	320	1	Heme (covalent; via 1 link)
Experimental info
	Mutagenesis	320	1	E → A: Loss of heme binding
	Sequence conflict	341	1	E → K in AAA41061. Ref.1
	Sequence conflict	341	1	E → K in CAA30245. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P08516-1 [UniParc]. Last modified May 1, 2007. Version 2. Checksum: E622FAB351BC40AF	FASTA	509	58,215
	10 20 30 40 50 60 MSVSALSSTR FTGSISGFLQ VASVLGLLLL LVKAVQFYLQ RQWLLKAFQQ FPSPPFHWFF 70 80 90 100 110 120 GHKQFQGDKE LQQIMTCVEN FPSAFPRWFW GSKAYLIVYD PDYMKVILGR SDPKANGVYR 130 140 150 160 170 180 LLAPWIGYGL LLLNGQPWFQ HRRMLTPAFH YDILKPYVKN MADSIRLMLD KWEQLAGQDS 190 200 210 220 230 240 SIEIFQHISL MTLDTVMKCA FSHNGSVQVD GNYKSYIQAI GNLNDLFHSR VRNIFHQNDT 250 260 270 280 290 300 IYNFSSNGHL FNRACQLAHD HTDGVIKLRK DQLQNAGELE KVKKKRRLDF LDILLLARME 310 320 330 340 350 360 NGDSLSDKDL RAEVDTFMFE GHDTTASGVS WIFYALATHP EHQQRCREEV QSVLGDGSSI 370 380 390 400 410 420 TWDHLDQIPY TTMCIKEALR LYPPVPGIVR ELSTSVTFPD GRSLPKGIQV TLSIYGLHHN 430 440 450 460 470 480 PKVWPNPEVF DPSRFAPDSP RHSHSFLPFS GGARNCIGKQ FAMSEMKVIV ALTLLRFELL 490 500 PDPTKVPIPL PRLVLKSKNG IYLYLKKLH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, complementary DNA sequence, and regulation of rat hepatic lauric acid omega-hydroxylase (cytochrome P-450LA omega). Identification of a new cytochrome P-450 gene family." Hardwick J.P., Song B.-J., Huberman E., Gonzalez F.J. J. Biol. Chem. 262:801-810(1987) [PubMed: 3027069] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Differential splicing in the 3' non-coding region of rat cytochrome P-452 (P450 IVA1) mRNA." Earnshaw D., Dale J.W., Goldfarb P.S., Gibson G.G. FEBS Lett. 236:357-361(1988) [PubMed: 3410047] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Liver.
[3]	"The rat clofibrate-inducible CYP4A gene subfamily. I. Complete intron and exon sequence of the CYP4A1 and CYP4A2 genes, unique exon organization, and identification of a conserved 19-bp upstream element." Kimura S., Hanioka N., Matsunaga E., Gonzalez F.J. DNA 8:503-516(1989) [PubMed: 2766932] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Liver.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[5]	"Characterization of a cytochrome P450 from di(2-ethylhexyl) phthalate-treated rats which hydroxylates fatty acids." Okita R.T., Okita J.R. Arch. Biochem. Biophys. 294:475-481(1992) [PubMed: 1567203] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-22.
[6]	"Covalently linked heme in cytochrome P4504A fatty acid hydroxylases." Hoch U., Ortiz de Montellano P.R. J. Biol. Chem. 276:11339-11346(2001) [PubMed: 11139583] [Abstract] Cited for: COVALENT HEME ATTACHMENT.
[7]	"Autocatalytic mechanism and consequences of covalent heme attachment in the cytochrome P4504A family." LeBrun L.A., Hoch U., Ortiz de Montellano P.R. J. Biol. Chem. 277:12755-12761(2002) [PubMed: 11821421] [Abstract] Cited for: COVALENT HEME ATTACHMENT, MUTAGENESIS OF GLU-320.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M14972 mRNA. Translation: AAA41061.1. X07259 mRNA. Translation: CAA30245.1. M57718 Genomic DNA. Translation: AAA41038.1. BC089761 mRNA. Translation: AAH89761.1.
PIR	O4RTLO. S01336.
RefSeq	NP_695219.1. NP_787031.1.
UniGene	Rn.92401
3D structure databases
HSSP	HSSP built from PDB template 1JPZ based on UniProtKB P14779.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000009597. Rattus norvegicus. [Contig view]
GeneID	170544. 50549.
KEGG	rno:170544. rno:50549.
Organism-specific databases
RGD	68945. Cyp4a1.
Phylogenomic databases
HOVERGEN	P08516.
Gene expression databases
ArrayExpress	P08516.
GermOnline	ENSRNOG00000009597. Rattus norvegicus.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	621028.

Entry information

Entry name

CP4AA_RAT

Accession

Primary (citable) accession number: P08516
Secondary accession number(s): Q5EBD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	May 1, 2007
Last modified:	November 25, 2008
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)