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Protein

Glutathione S-transferase class-mu 26 kDa isozyme

Gene
N/A
Organism
Schistosoma japonicum (Blood fluke)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei111 – 1111SubstrateCurated

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BRENDAi2.5.1.18. 5607.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase class-mu 26 kDa isozyme (EC:2.5.1.18)
Short name:
GST 26
Alternative name(s):
Sj26 antigen
SjGST
OrganismiSchistosoma japonicum (Blood fluke)
Taxonomic identifieri6182 [NCBI]
Taxonomic lineageiEukaryotaMetazoaPlatyhelminthesTrematodaDigeneaStrigeididaSchistosomatoideaSchistosomatidaeSchistosoma

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 218217Glutathione S-transferase class-mu 26 kDa isozymePRO_0000185805Add
BLAST

Proteomic databases

PRIDEiP08515.

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1
218
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 63Combined sources
Beta strandi9 – 113Combined sources
Helixi12 – 143Combined sources
Helixi15 – 217Combined sources
Beta strandi29 – 313Combined sources
Helixi35 – 373Combined sources
Helixi38 – 425Combined sources
Turni43 – 464Combined sources
Beta strandi55 – 595Combined sources
Beta strandi64 – 674Combined sources
Helixi68 – 725Combined sources
Helixi77 – 793Combined sources
Helixi86 – 10722Combined sources
Turni108 – 1114Combined sources
Helixi115 – 13622Combined sources
Turni137 – 1393Combined sources
Beta strandi145 – 1473Combined sources
Helixi150 – 16213Combined sources
Turni163 – 1653Combined sources
Turni167 – 1726Combined sources
Helixi174 – 18411Combined sources
Helixi187 – 1937Combined sources
Beta strandi195 – 1973Combined sources
Beta strandi203 – 2075Combined sources
Beta strandi209 – 2113Combined sources
Beta strandi213 – 2153Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1B8XX-ray2.70A2-218[»]
1BG5X-ray2.60A1-218[»]
1DUGX-ray1.80A/B2-218[»]
1GNEX-ray2.50A2-218[»]
1GTAX-ray2.40A1-218[»]
1GTBX-ray2.60A1-218[»]
1M99X-ray2.30A1-218[»]
1M9AX-ray2.10A1-218[»]
1M9BX-ray2.60A1-218[»]
1U87X-ray3.50A1-218[»]
1U88X-ray3.50A/B1-218[»]
1UA5X-ray2.50A1-218[»]
1Y6EX-ray3.00A/B2-218[»]
3CRTX-ray1.90A1-214[»]
3CRUX-ray2.30A1-214[»]
3D0ZX-ray2.50A1-214[»]
3QMZX-ray6.00S/T2-218[»]
4AI6X-ray3.40A/B2-218[»]
4AKGX-ray3.30A/B2-218[»]
4AKHX-ray3.60A/B2-218[»]
4AKIX-ray3.70A/B2-218[»]
4ECBX-ray2.20A/B1-218[»]
4ECCX-ray2.20A1-215[»]
4WR4X-ray1.60A2-218[»]
4WR5X-ray1.93A2-218[»]
ProteinModelPortaliP08515.
SMRiP08515. Positions 2-218.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08515.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 8382GST N-terminalAdd
BLAST
Domaini85 – 203119GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni7 – 82Glutathione binding1 Publication1 Publication
Regioni41 – 455Glutathione binding1 Publication1 Publication
Regioni54 – 552Glutathione binding1 Publication1 Publication
Regioni67 – 682Glutathione binding1 Publication1 Publication

Sequence similaritiesi

Belongs to the GST superfamily. Mu family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08515-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL
60 70 80 90 100
EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL
110 120 130 140 150
DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH
160 170 180 190 200
PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA
210
WPLQGWQATF GGGDHPPK
Length:218
Mass (Da):25,499
Last modified:January 23, 2007 - v3
Checksum:i5E2AC418BD0EF13F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14654 mRNA. Translation: AAB59203.1.
PIRiA61514.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14654 mRNA. Translation: AAB59203.1.
PIRiA61514.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1B8XX-ray2.70A2-218[»]
1BG5X-ray2.60A1-218[»]
1DUGX-ray1.80A/B2-218[»]
1GNEX-ray2.50A2-218[»]
1GTAX-ray2.40A1-218[»]
1GTBX-ray2.60A1-218[»]
1M99X-ray2.30A1-218[»]
1M9AX-ray2.10A1-218[»]
1M9BX-ray2.60A1-218[»]
1U87X-ray3.50A1-218[»]
1U88X-ray3.50A/B1-218[»]
1UA5X-ray2.50A1-218[»]
1Y6EX-ray3.00A/B2-218[»]
3CRTX-ray1.90A1-214[»]
3CRUX-ray2.30A1-214[»]
3D0ZX-ray2.50A1-214[»]
3QMZX-ray6.00S/T2-218[»]
4AI6X-ray3.40A/B2-218[»]
4AKGX-ray3.30A/B2-218[»]
4AKHX-ray3.60A/B2-218[»]
4AKIX-ray3.70A/B2-218[»]
4ECBX-ray2.20A/B1-218[»]
4ECCX-ray2.20A1-215[»]
4WR4X-ray1.60A2-218[»]
4WR5X-ray1.93A2-218[»]
ProteinModelPortaliP08515.
SMRiP08515. Positions 2-218.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP08515.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.5.1.18. 5607.

Miscellaneous databases

EvolutionaryTraceiP08515.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGST26_SCHJA
AccessioniPrimary (citable) accession number: P08515
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

There are at least two isoenzymes of GST in S.japonicum.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.