Reviewed, UniProtKB/Swiss-Prot P08515 (GST26_SCHJA)

Names and origin

Protein names	Recommended name:     Glutathione S-transferase class-mu 26 kDa isozyme       Short name=GST 26     EC=2.5.1.18 Alternative name(s):     Sj26 antigen     SjGST
Organism	Schistosoma japonicum (Blood fluke)
Taxonomic identifier	6182 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Platyhelminthes › Trematoda › Digenea › Strigeidida › Schistosomatoidea › Schistosomatidae › Schistosoma

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer.
Miscellaneous	There are at least two isoenzymes of GST in S.japonicum.
Sequence similarities	Belongs to the GST superfamily. Mu family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Molecular function	Transferase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.3

Chain

2 – 218

217

Glutathione S-transferase class-mu 26 kDa isozyme

PRO_0000185805

Regions

Domain

2 – 83

82

GST N-terminal

Domain

85 – 203

119

GST C-terminal

Sites

Active site

7

1

By similarity

Secondary structure

1

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218

Helix Strand Turn

Details...

Beta strand

4 – 11

8

Helix

12 – 14

3

Helix

15 – 24

10

Beta strand

29 – 33

5

Helix

38 – 44

7

Beta strand

55 – 59

5

Beta strand

64 – 67

4

Helix

68 – 78

11

Helix

86 – 110

25

Helix

115 – 136

22

Turn

137 – 139

3

Beta strand

145 – 147

3

Helix

150 – 165

16

Turn

167 – 172

6

Helix

174 – 185

12

Helix

187 – 193

7

Beta strand

209 – 211

3

Beta strand

213 – 215

3

Sequences

	Sequence		Length	Mass (Da)
	P08515-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 5E2AC418BD0EF13F	FASTA	218	25,499
	10 20 30 40 50 60 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 70 80 90 100 110 120 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 130 140 150 160 170 180 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 190 200 210 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPK

References

[1]	"Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase." Smith D.B., Davern K.M., Board P.G., Tiu W.U., Garcia E.G., Mitchell G.F. Proc. Natl. Acad. Sci. U.S.A. 83:8703-8707(1986) [PubMed: 3095841] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Smith D.B., Davern K.M., Board P.G., Tiu W.U., Garcia E.G., Mitchell G.F. Proc. Natl. Acad. Sci. U.S.A. 84:6541-6541(1987) Cited for: SEQUENCE REVISION.
[3]	"Expression of an enzymatically active parasite molecule in Escherichia coli: Schistosoma japonicum glutathione S-transferase." Smith D.B., Rubira M.R., Simpson R.J., Davern K.M., Tiu W.U., Board P.G., Mitchell G.F. Mol. Biochem. Parasitol. 27:249-256(1988) [PubMed: 3278228] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-15.
[4]	"Three-dimensional structure of Schistosoma japonicum glutathione S-transferase fused with a six-amino acid conserved neutralizing epitope of gp41 from HIV." Lim K., Ho J.X., Keeling K., Gilliland G.L., Ji X., Ruker F., Carter D.C. Protein Sci. 3:2233-2244(1994) [PubMed: 7538846] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
[5]	"Crystal structures of a schistosomal drug and vaccine target: glutathione S-transferase from Schistosoma japonica and its complex with the leading antischistosomal drug praziquantel." McTigue M.A., Williams D.R., Tainer J.A. J. Mol. Biol. 246:21-27(1995) [PubMed: 7853399] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M14654 mRNA. Translation: AAB59203.1.

PIR

A61514.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1B8X	X-ray	2.70	A	2-218	[»]
1BG5	X-ray	2.60	A	1-218	[»]
1DUG	X-ray	1.80	A/B	2-218	[»]
1GNE	X-ray	2.50	A	1-218	[»]
1GTA	X-ray	2.40	A	1-218	[»]
1GTB	X-ray	2.60	A	1-218	[»]
1M99	X-ray	2.30	A	1-218	[»]
1M9A	X-ray	2.10	A	1-218	[»]
1M9B	X-ray	2.60	A	1-218	[»]
1U87	X-ray	3.50	A	1-218	[»]
1U88	X-ray	3.50	A/B	1-218	[»]
1UA5	X-ray	2.50	A	1-218	[»]
1Y6E	X-ray	3.00	A/B	2-217	[»]
3CRT	X-ray	1.90	A	1-214	[»]
3CRU	X-ray	2.30	A	1-214	[»]
3D0Z	X-ray	2.50	A	1-214	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

2.5.1.18. 1465.

Family and domain databases

InterPro

IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR017933. Glutathione_S_Trfase/Cl_chnl_C.
IPR004046. GST_C.
IPR012335. Thioredoxin_fold.
[Graphical view]

Gene3D

G3DSA:1.20.1050.10. GST_C_like. 1 hit.
G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.

Pfam

PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]

PROSITE

PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

DrugBank

DB00143. Glutathione.
DB01058. Praziquantel.

Entry information

Entry name

GST26_SCHJA

Accession

Primary (citable) accession number: P08515

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 81 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)