P08510 (KCNAS_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium voltage-gated channel protein Shaker

Alternative name(s):
Protein minisleep

Gene names

Name:	Sh
Synonyms:	mns
ORF Names:	CG12348

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	655 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Voltage-dependent potassium channel involved in regulation of sleep need or efficiency. Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. Ref.12
Subunit structure	The voltage-dependent potassium channel is a heterotetramer of potassium channel proteins By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Domain	The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
Disruption phenotype	Reduced sleep. Flies sleep for one-third of the wild-type amount. They perform normally in a number of tasks, have preserved sleep homeostasis, but are not impaired by sleep deprivation. They also have a reduced lifespan. Ref.12
Sequence similarities	Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Shaker sub-subfamily. [View classification]
RNA editing	Edited at positions 178, 360, 464, 489 and 491. Partially edited. Ref.1 Ref.3 Ref.8 Ref.11
Sequence caution	The sequence AAA70217.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing RNA editing
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Potassium channel Voltage-gated channel
PTM	Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axon extension Inferred from mutant phenotype PubMed 14960616. Source: FlyBase behavioral response to ether Non-traceable author statement PubMed 11715043. Source: FlyBase courtship behavior Non-traceable author statement PubMed 11715043. Source: FlyBase detection of visible light Inferred from mutant phenotype PubMed 16168982. Source: FlyBase flight behavior Inferred from mutant phenotype PubMed 17248760. Source: FlyBase larval locomotory behavior Inferred from mutant phenotype PubMed 16763031. Source: FlyBase learning or memory Non-traceable author statement PubMed 11715043. Source: FlyBase mating behavior, sex discrimination Inferred from direct assay PubMed 22292044. Source: FlyBase proboscis extension reflex Inferred from mutant phenotype PubMed 16121192. Source: FlyBase protein homooligomerization Inferred from electronic annotation. Source: InterPro regulation of action potential Inferred from mutant phenotype PubMed 16763031. Source: FlyBase regulation of circadian sleep/wake cycle, sleep Inferred from mutant phenotype PubMed 16753559. Source: FlyBase regulation of synaptic activity Inferred from mutant phenotype PubMed 16763031. Source: FlyBase sensory perception of taste Non-traceable author statement PubMed 11715043. Source: FlyBase sleep Inferred from mutant phenotype PubMed 19038223. Source: FlyBase
Cellular_component	voltage-gated potassium channel complex Inferred from physical interaction PubMed 8968587. Source: FlyBase
Molecular_function	delayed rectifier potassium channel activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]

Isoform E (identifier: P08510-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Alpha (identifier: P08510-2)

Also known as: B; G; Adult;

The sequence of this isoform differs from the canonical sequence as follows:
     453-463: VWGKIVGSLCA → FWGKIVGSLCV
     506-655: PYLPGTLGQH...ALAVSIETDV → SYLPGALGQH...AMAVSIETDV

Isoform Beta (identifier: P08510-3)

Also known as: C; Late population;

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: MAAVAGLYGL...LQRNSLDGYG → MTMWQSGGMG...SGSNERNLNQ

Isoform Gamma (identifier: P08510-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: MAAVAGLYGLGEDRQHRKKQQQQQQHQKEQLEQKEEQKKIAERKLQLREQQLQRNSLDGYG → MQMILVAGG
     506-655: PYLPGTLGQH...ALAVSIETDV → SYLPGALGQH...AMAVSIETDV

Isoform Delta (identifier: P08510-5)

Also known as: Larval; A;

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: MAAVAGLYGL...LQRNSLDGYG → MAHITTTHGSLSQATR
     349-349: D → V
     350-655: Missing.

Isoform Epsilon (identifier: P08510-6)

Also known as: Larval; F;

The sequence of this isoform differs from the canonical sequence as follows:
     349-349: D → V
     350-655: Missing.

Isoform D (identifier: P08510-7)

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: MAAVAGLYGL...LQRNSLDGYG → MAHITTTHGSLSQATR
     453-463: VWGKIVGSLCA → FWGKIVGSLCV
     506-655: PYLPGTLGQH...ALAVSIETDV → SYLPGALGQH...AMAVSIETDV

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 655

655

Potassium voltage-gated channel protein Shaker

PRO_0000053963

Regions

Topological domain

1 – 227

227

Cytoplasmic Potential

Transmembrane

228 – 246

19

Helical; Name=Segment S1

Topological domain

247 – 278

32

Extracellular Potential

Transmembrane

279 – 300

22

Helical; Name=Segment S2

Topological domain

301 – 311

11

Cytoplasmic Potential

Transmembrane

312 – 332

21

Helical; Name=Segment S3

Topological domain

333 – 360

28

Extracellular Potential

Transmembrane

361 – 380

20

Helical; Voltage-sensor; Name=Segment S4

Topological domain

381 – 395

15

Cytoplasmic Potential

Transmembrane

396 – 415

20

Helical; Name=Segment S5

Transmembrane

457 – 478

22

Helical; Name=Segment S6

Topological domain

479 – 616

138

Cytoplasmic Potential

Motif

442 – 447

6

Selectivity filter

Compositional bias

15 – 53

39

Gln-rich

Compositional bias

558 – 619

62

Gln-rich

Compositional bias

625 – 630

6

Poly-Ala

Amino acid modifications

Glycosylation

259

1

N-linked (GlcNAc...) Ref.13

Glycosylation

263

1

N-linked (GlcNAc...) Ref.13

Natural variations

Alternative sequence

1 – 61

61

MAAVA…LDGYG → MTMWQSGGMGGHGSQNNPWM KLMGIVHKERRHTENVQSQS GSNERNLNQ in isoform Beta.

VSP_000954

Alternative sequence

1 – 61

61

MAAVA…LDGYG → MAHITTTHGSLSQATR in isoform Delta and isoform D.

VSP_000956

Alternative sequence

1 – 61

61

MAAVA…LDGYG → MQMILVAGG in isoform Gamma.

VSP_000955

Alternative sequence

349

1

D → V in isoform Delta and isoform Epsilon.

VSP_000957

Alternative sequence

350 – 655

306

Missing in isoform Delta and isoform Epsilon.

VSP_000958

Alternative sequence

453 – 463

11

VWGKIVGSLCA → FWGKIVGSLCV in isoform Alpha and isoform D.

VSP_017889

Alternative sequence

506 – 655

150

PYLPG…IETDV → SYLPGALGQHLKKSSLSESS SDIMDLDDGIDATTPGLTDH TGRHMVPFLRTQQSFEKQQL QLQLQLQQQSQSPHGQQMTQ QQQLGQNGLRSTNSLQLRHN NAMAVSIETDV in isoform Alpha, isoform Gamma and isoform D.

VSP_000959

Natural variant

178

1

K → E in RNA edited version.

Natural variant

178

1

K → G in RNA edited version.

Natural variant

178

1

K → R in RNA edited version.

Natural variant

360

1

I → M in RNA edited version.

Natural variant

464

1

I → V in RNA edited version.

Natural variant

489

1

T → A in RNA edited version.

Natural variant

491

1

Q → R in RNA edited version.

Experimental info

Mutagenesis

248

1

T → I in mns; flies display reduced sleep. Ref.12

Sequence conflict

563

1

Q → QQ in CAA29917. Ref.4

Sequence conflict

583 – 584

2

QL → HV in CAA29917. Ref.4

Secondary structure

1

.

.

.

655

Helix Strand Turn

Details...

Helix

380 – 394

15

Sequences

	Sequence		Length	Mass (Da)
	Isoform E [UniParc]. Last modified April 4, 2006. Version 3. Checksum: 5833E3052C9D19B3	FASTA	655	74,193
	10 20 30 40 50 60 MAAVAGLYGL GEDRQHRKKQ QQQQQHQKEQ LEQKEEQKKI AERKLQLREQ QLQRNSLDGY 70 80 90 100 110 120 GSLPKLSSQD EEGGAGHGFG GGPQHFEPIP HDHDFCERVV INVSGLRFET QLRTLNQFPD 130 140 150 160 170 180 TLLGDPARRL RYFDPLRNEY FFDRSRPSFD AILYYYQSGG RLRRPVNVPL DVFSEEIKFY 190 200 210 220 230 240 ELGDQAINKF REDEGFIKEE ERPLPDNEKQ RKVWLLFEYP ESSQAARVVA IISVFVILLS 250 260 270 280 290 300 IVIFCLETLP EFKHYKVFNT TTNGTKIEED EVPDITDPFF LIETLCIIWF TFELTVRFLA 310 320 330 340 350 360 CPNKLNFCRD VMNVIDIIAI IPYFITLATV VAEEEDTLNL PKAPVSPQDK SSNQAMSLAI 370 380 390 400 410 420 LRVIRLVRVF RIFKLSRHSK GLQILGRTLK ASMRELGLLI FFLFIGVVLF SSAVYFAEAG 430 440 450 460 470 480 SENSFFKSIP DAFWWAVVTM TTVGYGDMTP VGVWGKIVGS LCAIAGVLTI ALPVPVIVSN 490 500 510 520 530 540 FNYFYHRETD QEEMQSQNFN HVTSCPYLPG TLGQHMKKSS LSESSSDMMD LDDGVESTPG 550 560 570 580 590 600 LTETHPGRSA VAPFLGAQQQ QQQPVASSLS MSIDKQLQHP LQQLTQTQLY QQQQQQQQQQ 610 620 630 640 650 QNGFKQQQQQ TQQQLQQQQS HTINASAAAA TSGSGSSGLT MRHNNALAVS IETDV
	Isoform Alpha (B) (G) (Adult) [UniParc]. Checksum: B6453435442CB513	FASTA	616	70,281
	10 20 30 40 50 60 MAAVAGLYGL GEDRQHRKKQ QQQQQHQKEQ LEQKEEQKKI AERKLQLREQ QLQRNSLDGY 70 80 90 100 110 120 GSLPKLSSQD EEGGAGHGFG GGPQHFEPIP HDHDFCERVV INVSGLRFET QLRTLNQFPD 130 140 150 160 170 180 TLLGDPARRL RYFDPLRNEY FFDRSRPSFD AILYYYQSGG RLRRPVNVPL DVFSEEIKFY 190 200 210 220 230 240 ELGDQAINKF REDEGFIKEE ERPLPDNEKQ RKVWLLFEYP ESSQAARVVA IISVFVILLS 250 260 270 280 290 300 IVIFCLETLP EFKHYKVFNT TTNGTKIEED EVPDITDPFF LIETLCIIWF TFELTVRFLA 310 320 330 340 350 360 CPNKLNFCRD VMNVIDIIAI IPYFITLATV VAEEEDTLNL PKAPVSPQDK SSNQAMSLAI 370 380 390 400 410 420 LRVIRLVRVF RIFKLSRHSK GLQILGRTLK ASMRELGLLI FFLFIGVVLF SSAVYFAEAG 430 440 450 460 470 480 SENSFFKSIP DAFWWAVVTM TTVGYGDMTP VGFWGKIVGS LCVIAGVLTI ALPVPVIVSN 490 500 510 520 530 540 FNYFYHRETD QEEMQSQNFN HVTSCSYLPG ALGQHLKKSS LSESSSDIMD LDDGIDATTP 550 560 570 580 590 600 GLTDHTGRHM VPFLRTQQSF EKQQLQLQLQ LQQQSQSPHG QQMTQQQQLG QNGLRSTNSL 610 QLRHNNAMAV SIETDV
	Isoform Beta (C) (Late population) [UniParc]. Checksum: E1F358B5555496D2	FASTA	643	72,501
	10 20 30 40 50 60 MTMWQSGGMG GHGSQNNPWM KLMGIVHKER RHTENVQSQS GSNERNLNQS LPKLSSQDEE 70 80 90 100 110 120 GGAGHGFGGG PQHFEPIPHD HDFCERVVIN VSGLRFETQL RTLNQFPDTL LGDPARRLRY 130 140 150 160 170 180 FDPLRNEYFF DRSRPSFDAI LYYYQSGGRL RRPVNVPLDV FSEEIKFYEL GDQAINKFRE 190 200 210 220 230 240 DEGFIKEEER PLPDNEKQRK VWLLFEYPES SQAARVVAII SVFVILLSIV IFCLETLPEF 250 260 270 280 290 300 KHYKVFNTTT NGTKIEEDEV PDITDPFFLI ETLCIIWFTF ELTVRFLACP NKLNFCRDVM 310 320 330 340 350 360 NVIDIIAIIP YFITLATVVA EEEDTLNLPK APVSPQDKSS NQAMSLAILR VIRLVRVFRI 370 380 390 400 410 420 FKLSRHSKGL QILGRTLKAS MRELGLLIFF LFIGVVLFSS AVYFAEAGSE NSFFKSIPDA 430 440 450 460 470 480 FWWAVVTMTT VGYGDMTPVG VWGKIVGSLC AIAGVLTIAL PVPVIVSNFN YFYHRETDQE 490 500 510 520 530 540 EMQSQNFNHV TSCPYLPGTL GQHMKKSSLS ESSSDMMDLD DGVESTPGLT ETHPGRSAVA 550 560 570 580 590 600 PFLGAQQQQQ QPVASSLSMS IDKQLQHPLQ QLTQTQLYQQ QQQQQQQQQN GFKQQQQQTQ 610 620 630 640 QQLQQQQSHT INASAAAATS GSGSSGLTMR HNNALAVSIE TDV
	Isoform Gamma [UniParc]. Checksum: 24355C070CF1A405	FASTA	564	63,865
	10 20 30 40 50 60 MQMILVAGGS LPKLSSQDEE GGAGHGFGGG PQHFEPIPHD HDFCERVVIN VSGLRFETQL 70 80 90 100 110 120 RTLNQFPDTL LGDPARRLRY FDPLRNEYFF DRSRPSFDAI LYYYQSGGRL RRPVNVPLDV 130 140 150 160 170 180 FSEEIKFYEL GDQAINKFRE DEGFIKEEER PLPDNEKQRK VWLLFEYPES SQAARVVAII 190 200 210 220 230 240 SVFVILLSIV IFCLETLPEF KHYKVFNTTT NGTKIEEDEV PDITDPFFLI ETLCIIWFTF 250 260 270 280 290 300 ELTVRFLACP NKLNFCRDVM NVIDIIAIIP YFITLATVVA EEEDTLNLPK APVSPQDKSS 310 320 330 340 350 360 NQAMSLAILR VIRLVRVFRI FKLSRHSKGL QILGRTLKAS MRELGLLIFF LFIGVVLFSS 370 380 390 400 410 420 AVYFAEAGSE NSFFKSIPDA FWWAVVTMTT VGYGDMTPVG VWGKIVGSLC AIAGVLTIAL 430 440 450 460 470 480 PVPVIVSNFN YFYHRETDQE EMQSQNFNHV TSCSYLPGAL GQHLKKSSLS ESSSDIMDLD 490 500 510 520 530 540 DGIDATTPGL TDHTGRHMVP FLRTQQSFEK QQLQLQLQLQ QQSQSPHGQQ MTQQQQLGQN 550 560 GLRSTNSLQL RHNNAMAVSI ETDV
	Isoform Delta (Larval) (A) [UniParc]. Checksum: 05898ADD605604B5	FASTA	304	34,946
	10 20 30 40 50 60 MAHITTTHGS LSQATRSLPK LSSQDEEGGA GHGFGGGPQH FEPIPHDHDF CERVVINVSG 70 80 90 100 110 120 LRFETQLRTL NQFPDTLLGD PARRLRYFDP LRNEYFFDRS RPSFDAILYY YQSGGRLRRP 130 140 150 160 170 180 VNVPLDVFSE EIKFYELGDQ AINKFREDEG FIKEEERPLP DNEKQRKVWL LFEYPESSQA 190 200 210 220 230 240 ARVVAIISVF VILLSIVIFC LETLPEFKHY KVFNTTTNGT KIEEDEVPDI TDPFFLIETL 250 260 270 280 290 300 CIIWFTFELT VRFLACPNKL NFCRDVMNVI DIIAIIPYFI TLATVVAEEE DTLNLPKAPV SPQV
	Isoform Epsilon (Larval) (F) [UniParc]. Checksum: B509504C2D3EE550	FASTA	349	40,493
	10 20 30 40 50 60 MAAVAGLYGL GEDRQHRKKQ QQQQQHQKEQ LEQKEEQKKI AERKLQLREQ QLQRNSLDGY 70 80 90 100 110 120 GSLPKLSSQD EEGGAGHGFG GGPQHFEPIP HDHDFCERVV INVSGLRFET QLRTLNQFPD 130 140 150 160 170 180 TLLGDPARRL RYFDPLRNEY FFDRSRPSFD AILYYYQSGG RLRRPVNVPL DVFSEEIKFY 190 200 210 220 230 240 ELGDQAINKF REDEGFIKEE ERPLPDNEKQ RKVWLLFEYP ESSQAARVVA IISVFVILLS 250 260 270 280 290 300 IVIFCLETLP EFKHYKVFNT TTNGTKIEED EVPDITDPFF LIETLCIIWF TFELTVRFLA 310 320 330 340 CPNKLNFCRD VMNVIDIIAI IPYFITLATV VAEEEDTLNL PKAPVSPQV
	Isoform D [UniParc]. Checksum: 7CFF19956D54D599	FASTA	571	64,734
	10 20 30 40 50 60 MAHITTTHGS LSQATRSLPK LSSQDEEGGA GHGFGGGPQH FEPIPHDHDF CERVVINVSG 70 80 90 100 110 120 LRFETQLRTL NQFPDTLLGD PARRLRYFDP LRNEYFFDRS RPSFDAILYY YQSGGRLRRP 130 140 150 160 170 180 VNVPLDVFSE EIKFYELGDQ AINKFREDEG FIKEEERPLP DNEKQRKVWL LFEYPESSQA 190 200 210 220 230 240 ARVVAIISVF VILLSIVIFC LETLPEFKHY KVFNTTTNGT KIEEDEVPDI TDPFFLIETL 250 260 270 280 290 300 CIIWFTFELT VRFLACPNKL NFCRDVMNVI DIIAIIPYFI TLATVVAEEE DTLNLPKAPV 310 320 330 340 350 360 SPQDKSSNQA MSLAILRVIR LVRVFRIFKL SRHSKGLQIL GRTLKASMRE LGLLIFFLFI 370 380 390 400 410 420 GVVLFSSAVY FAEAGSENSF FKSIPDAFWW AVVTMTTVGY GDMTPVGFWG KIVGSLCVIA 430 440 450 460 470 480 GVLTIALPVP VIVSNFNYFY HRETDQEEMQ SQNFNHVTSC SYLPGALGQH LKKSSLSESS 490 500 510 520 530 540 SDIMDLDDGI DATTPGLTDH TGRHMVPFLR TQQSFEKQQL QLQLQLQQQS QSPHGQQMTQ 550 560 570 QQQLGQNGLR STNSLQLRHN NAMAVSIETD V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of a probable potassium channel component encoded at Shaker locus of Drosophila." Tempel B.L., Papazian D.M., Schwarz T.L., Jan Y.N., Jan L.Y. Science 237:770-775(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA), RNA EDITING OF POSITIONS 464; 489 AND 491.
[2]	"Molecular organization of the maternal effect region of the Shaker complex of Drosophila: characterization of an I(A) channel transcript with homology to vertebrate Na(+) channel." Baumann A., Krah-Jentgens I., Mueller R., Mueller-Holtkamp F., Seidel R., Kecskemethy N., Casal J., Ferrus A., Pongs O. EMBO J. 6:3419-3429(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM DELTA). Strain: Canton-S and Oregon-R.
[3]	"Shaker encodes a family of putative potassium channel proteins in the nervous system of Drosophila." Pongs O., Kecskemethy N., Mueller R., Krah-Jentgens I., Baumann A., Kiltz H.H., Canal I., Llamazares S., Ferrus A. EMBO J. 7:1087-1096(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA; BETA; DELTA; GAMMA AND EPSILON), RNA EDITING OF POSITIONS 464; 489 AND 491. Strain: Canton-S. Tissue: Larva.
[4]	"Multiple potassium-channel components are produced by alternative splicing at the Shaker locus in Drosophila." Schwarz T.L., Tempel B.L., Papazian D.M., Jan Y.N., Jan L.Y. Nature 331:137-142(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA; BETA; GAMMA AND E).
[5]	Erratum Schwarz T.L., Tempel B.L., Papazian D.M., Jan Y.N., Jan L.Y. Nature 332:740-740(1988)
[6]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[7]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[8]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM D), RNA EDITING OF POSITION 360. Strain: Berkeley. Tissue: Embryo.
[9]	"Molecular characterization of Shaker, a Drosophila gene that encodes a potassium channel." Kamb A., Iverson L.E., Tanouye M.A. Cell 50:405-413(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-257 (ISOFORM BETA). Tissue: Larva.
[10]	"Multiple products of the Drosophila Shaker gene may contribute to potassium channel diversity." Kamb A., Tseng-Crank J., Tanouye M.A. Neuron 1:421-430(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 450-534. Strain: H4.
[11]	"Nervous system targets of RNA editing identified by comparative genomics." Hoopengardner B., Bhalla T., Staber C., Reenan R. Science 301:832-836(2003) [PubMed] [Europe PMC] [Abstract] Cited for: RNA EDITING.
[12]	"Reduced sleep in Drosophila Shaker mutants." Cirelli C., Bushey D., Hill S., Huber R., Kreber R., Ganetzky B., Tononi G. Nature 434:1087-1092(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF THR-248.
[13]	"Identification of N-glycosylated proteins from the central nervous system of Drosophila melanogaster." Koles K., Lim J.-M., Aoki K., Porterfield M., Tiemeyer M., Wells L., Panin V. Glycobiology 17:1388-1403(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-259 AND ASN-263, MASS SPECTROMETRY. Strain: Oregon-R. Tissue: Head.
[14]	"Three-dimensional structure of the S4-S5 segment of the Shaker potassium channel." Ohlenschlaeger O., Hojo H., Ramachandran R., Goerlach M., Haris P.I. Biophys. J. 82:2995-3002(2002) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 378-397.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M17211 mRNA. Translation: AAA28417.1.
X06184 mRNA. Translation: CAA29549.1.
X07131 mRNA. Translation: CAA30143.1.
X07132 mRNA. Translation: CAA30144.1.
X07133 mRNA. Translation: CAA30145.1.
X07134 mRNA. Translation: CAA30146.1.
X06742 mRNA. Translation: CAA29917.1.
AE014298 Genomic DNA. Translation: AAF48785.3.
AE014298 Genomic DNA. Translation: AAF48786.3.
AE014298 Genomic DNA. Translation: AAF48790.2.
AE014298 Genomic DNA. Translation: AAN09451.1.
AE014298 Genomic DNA. Translation: AAN09452.1.
AE014298 Genomic DNA. Translation: AAS65395.1.
AE014298 Genomic DNA. Translation: AAS65396.1.
AY118398 mRNA. Translation: AAM48427.1.
M17155 mRNA. Translation: AAA70217.1. Frameshift.
X78908 Genomic DNA. Translation: CAA55519.1.

PIR

A27159.
JH0193.
S00480.
S02284.

RefSeq

NP_001162788.1. NM_001169317.1.
NP_001188668.1. NM_001201739.1.
NP_523393.3. NM_078669.5.
NP_728120.1. NM_167592.4.
NP_728122.1. NM_167594.3.
NP_728123.1. NM_167595.4.
NP_728124.1. NM_167596.4.
NP_996497.1. NM_206774.2.
NP_996498.2. NM_206775.4.

UniGene

Dm.7088.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HO2	NMR	-	A	378-397	[»]
1HO7	NMR	-	A	378-397	[»]

DisProt

DP00267.

ProteinModelPortal

P08510.

SMR

P08510. Positions 96-489.

ModBase

Search...

Protein-protein interaction databases

IntAct

P08510. 5 interactions.

MINT

MINT-317832.

Protein family/group databases

TCDB

1.A.1.2.6. voltage-gated ion channel (VIC) superfamily.

Proteomic databases

PaxDb

P08510.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

FBtr0089657; FBpp0088599; FBgn0003380.

GeneID

32780.

KEGG

dme:Dmel_CG12348.

Organism-specific databases

CTD

32780.

FlyBase

FBgn0003380. Sh.

Phylogenomic databases

eggNOG

COG1226.

GeneTree

ENSGT00560000076957.

InParanoid

P08510.

KO

K05318.

OMA

SERIIIN.

OrthoDB

EOG4KD52H.

PhylomeDB

P08510.

Gene expression databases

Bgee

P08510.

GermOnline

CG12348. Drosophila melanogaster.

Family and domain databases

Gene3D

3.30.710.10. 1 hit.

InterPro

IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR003131. T1-type_BTB.
[Graphical view]

PANTHER

PTHR11537. PTHR11537. 1 hit.

Pfam

PF00520. Ion_trans. 1 hit.
PF02214. K_tetra. 1 hit.
[Graphical view]

PRINTS

PR00169. KCHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.

SMART

SM00225. BTB. 1 hit.
[Graphical view]

SUPFAM

SSF54695. BTB/POZ_fold. 1 hit.

ProtoNet

Search...

Other

ChiTaRS

Sh. drosophila.

EvolutionaryTrace

P08510.

GenomeRNAi

32780.

NextBio

780331.

Entry information

Entry name

KCNAS_DROME

Accession

Primary (citable) accession number: P08510
Secondary accession number(s): A4V4Q2 , P08511, P08512, P08513, Q24277, Q24521, Q7KUW5, Q8IQY9, Q8MT41, Q9VWZ5, Q9VWZ9, Q9VX00

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	April 4, 2006
Last modified:	April 3, 2013
This is version 155 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project