Reviewed, UniProtKB/Swiss-Prot P08507 (GPDA_RABIT)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic
      Short name=GPDH-C
      Short name=GPD-C
    EC=1.1.1.8

Gene names

Name:

GPD1

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC protein homodimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 349	348	Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic		PRO_0000138081
Regions
	Nucleotide binding	10 – 15	6	NAD By similarity
	Region	269 – 270	2	Substrate binding By similarity
Sites
	Active site	204	1	Proton acceptor By similarity
	Binding site	97	1	NAD By similarity
	Binding site	120	1	NAD; via amide nitrogen By similarity
	Binding site	120	1	Substrate By similarity
	Binding site	153	1	NAD; via amide nitrogen By similarity
	Binding site	269	1	NAD By similarity
	Binding site	298	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08507-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: A11FE0E1F4ADD807	FASTA	349	37,610
	10 20 30 40 50 60 MAGKKVCIVG SGDWGSAIAK IVGGNAAQLA QFDPRVTMWV FEEDIGGKKL TEIINTHQEN 70 80 90 100 110 120 VKYLPGHKLP PNVVAVPDVV KAAADADILI FVVPHQFIGK ICDEIKGHLK ANAIGISLIK 130 140 150 160 170 180 GVNEGPKGLK LISEVIGEHL GIPMSVLMGA NIASEVADEK FCETTIGCKD QAQGQLLKQL 190 200 210 220 230 240 MQTPNFRIVV TQEVNTVEIC GALKDLVAVG AGFCDGIGFG DNTKAAVIRL GLMEMIAFAK 250 260 270 280 290 300 LFCSGPVSPA TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE MLNGQKLQGP 310 320 330 340 ETARELHSIL QHKGLVDWFP LFMAVYKVCY QGQPVGEFIR CLQNHPEHM

References

[1]	"Prediction of secondary structural elements in glycerol-3-phosphate dehydrogenase by comparison with other dehydrogenases." Otto J., Argos P., Rossmann M.G. Eur. J. Biochem. 109:325-330(1980) [PubMed: 6773774] [Abstract] Cited for: SECONDARY STRUCTURE PREDICTION.

Cross-references

Sequence databases
PIR	A32512.
3D structure databases
HSSP	HSSP built from PDB template 1EVY based on UniProtKB P90551.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P08507.
Enzyme and pathway databases
BRENDA	1.1.1.8. 255.
Family and domain databases
InterPro	IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. IPR006168. NAD-dep_Gly3P_DH. IPR017751. NAD-dep_Gly3P_DH_euk. IPR011128. NAD-dep_Gly3P_DH_N. IPR006109. NAD_Gly3P_DH_C. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR11728. NAD_Gly3P_DH. 1 hit.
Pfam	PF07479. NAD_Gly3P_dh_C. 1 hit. PF01210. NAD_Gly3P_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTS	PR00077. GPDHDRGNASE.
ProDom	PD001278. NAD_Gly3P_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03376. glycerol3P_DH. 1 hit.
PROSITE	PS00957. NAD_G3PDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPDA_RABIT

Accession

Primary (citable) accession number: P08507

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)