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Protein

Interleukin-6

Gene

Il6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation.1 Publication

GO - Molecular functioni

  • cytokine activity Source: MGI
  • growth factor activity Source: MGI
  • interleukin-6 receptor binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Growth factor

Keywords - Biological processi

Acute phase

Enzyme and pathway databases

ReactomeiREACT_277805. ERK1 activation.
REACT_301070. Interleukin-6 signaling.
REACT_350665. Senescence-Associated Secretory Phenotype (SASP).
REACT_353300. ERK2 activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-6
Short name:
IL-6
Alternative name(s):
B-cell hybridoma growth factor
Interleukin HP-1
Gene namesi
Name:Il6
Synonyms:Il-6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:96559. Il6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Animals have normal T-cell numbers in the lamina propria but the T(H)17 cells are reduced by about 10-fold.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24242 PublicationsAdd
BLAST
Chaini25 – 211187Interleukin-6PRO_0000015588Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi70 ↔ 761 Publication
Disulfide bondi99 ↔ 1091 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP08505.
PRIDEiP08505.

PTM databases

PhosphoSiteiP08505.

Expressioni

Gene expression databases

BgeeiP08505.
CleanExiMM_IL6.
ExpressionAtlasiP08505. baseline and differential.
GenevisibleiP08505. MM.

Interactioni

Protein-protein interaction databases

BioGridi200641. 2 interactions.
IntActiP08505. 1 interaction.
STRINGi10090.ENSMUSP00000026845.

Structurei

Secondary structure

1
211
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi48 – 6821Combined sources
Turni69 – 724Combined sources
Helixi74 – 774Combined sources
Turni81 – 833Combined sources
Helixi84 – 863Combined sources
Beta strandi101 – 1033Combined sources
Helixi114 – 12916Combined sources
Helixi133 – 16028Combined sources
Helixi170 – 1767Combined sources
Helixi186 – 20924Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L3YNMR-A27-211[»]
ProteinModelPortaliP08505.
SMRiP08505. Positions 28-211.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08505.

Family & Domainsi

Sequence similaritiesi

Belongs to the IL-6 superfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG48083.
GeneTreeiENSGT00390000000878.
HOGENOMiHOG000236330.
HOVERGENiHBG000471.
InParanoidiP08505.
KOiK05405.
OMAiFNQETCL.
OrthoDBiEOG7QG453.
PhylomeDBiP08505.
TreeFamiTF335984.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR003574. IL-6.
IPR030474. IL-6/GCSF/MGF.
IPR003573. IL-6/IL-23/GCSF/MGF.
IPR030473. IL6/GCSF/MGF_CS.
[Graphical view]
PANTHERiPTHR11457. PTHR11457. 1 hit.
PfamiPF00489. IL6. 1 hit.
[Graphical view]
PIRSFiPIRSF001935. IL6_MGF_GCSF. 1 hit.
PRINTSiPR00433. IL6GCSFMGF.
SMARTiSM00126. IL6. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00254. INTERLEUKIN_6. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08505-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFLSARDFH PVAFLGLMLV TTTAFPTSQV RRGDFTEDTT PNRPVYTTSQ
60 70 80 90 100
VGGLITHVLW EIVEMRKELC NGNSDCMNND DALAENNLKL PEIQRNDGCY
110 120 130 140 150
QTGYNQEICL LKISSGLLEY HSYLEYMKNN LKDNKKDKAR VLQRDTETLI
160 170 180 190 200
HIFNQEVKDL HKIVLPTPIS NALLTDKLES QKEWLRTKTI QFILKSLEEF
210
LKVTLRSTRQ T
Length:211
Mass (Da):24,384
Last modified:August 1, 1988 - v1
Checksum:iBBB47DDA9E86787A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06203 mRNA. Translation: CAA29560.1.
M20572 Genomic DNA. Translation: AAA39302.1.
J03783 mRNA. Translation: AAA39301.1.
X54542 mRNA. Translation: CAA38411.1.
AK150440 mRNA. Translation: BAE29562.1.
X51457 Genomic DNA. Translation: CAA35824.1.
M24221 Genomic DNA. Translation: AAA68814.1.
CCDSiCCDS19153.1.
PIRiA30531. ICMS6.
RefSeqiNP_112445.1. NM_031168.1.
UniGeneiMm.1019.

Genome annotation databases

EnsembliENSMUST00000026845; ENSMUSP00000026845; ENSMUSG00000025746.
GeneIDi16193.
KEGGimmu:16193.
UCSCiuc008wuu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06203 mRNA. Translation: CAA29560.1.
M20572 Genomic DNA. Translation: AAA39302.1.
J03783 mRNA. Translation: AAA39301.1.
X54542 mRNA. Translation: CAA38411.1.
AK150440 mRNA. Translation: BAE29562.1.
X51457 Genomic DNA. Translation: CAA35824.1.
M24221 Genomic DNA. Translation: AAA68814.1.
CCDSiCCDS19153.1.
PIRiA30531. ICMS6.
RefSeqiNP_112445.1. NM_031168.1.
UniGeneiMm.1019.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L3YNMR-A27-211[»]
ProteinModelPortaliP08505.
SMRiP08505. Positions 28-211.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200641. 2 interactions.
IntActiP08505. 1 interaction.
STRINGi10090.ENSMUSP00000026845.

PTM databases

PhosphoSiteiP08505.

Proteomic databases

MaxQBiP08505.
PRIDEiP08505.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026845; ENSMUSP00000026845; ENSMUSG00000025746.
GeneIDi16193.
KEGGimmu:16193.
UCSCiuc008wuu.1. mouse.

Organism-specific databases

CTDi3569.
MGIiMGI:96559. Il6.

Phylogenomic databases

eggNOGiNOG48083.
GeneTreeiENSGT00390000000878.
HOGENOMiHOG000236330.
HOVERGENiHBG000471.
InParanoidiP08505.
KOiK05405.
OMAiFNQETCL.
OrthoDBiEOG7QG453.
PhylomeDBiP08505.
TreeFamiTF335984.

Enzyme and pathway databases

ReactomeiREACT_277805. ERK1 activation.
REACT_301070. Interleukin-6 signaling.
REACT_350665. Senescence-Associated Secretory Phenotype (SASP).
REACT_353300. ERK2 activation.

Miscellaneous databases

EvolutionaryTraceiP08505.
NextBioi289109.
PROiP08505.
SOURCEiSearch...

Gene expression databases

BgeeiP08505.
CleanExiMM_IL6.
ExpressionAtlasiP08505. baseline and differential.
GenevisibleiP08505. MM.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR003574. IL-6.
IPR030474. IL-6/GCSF/MGF.
IPR003573. IL-6/IL-23/GCSF/MGF.
IPR030473. IL6/GCSF/MGF_CS.
[Graphical view]
PANTHERiPTHR11457. PTHR11457. 1 hit.
PfamiPF00489. IL6. 1 hit.
[Graphical view]
PIRSFiPIRSF001935. IL6_MGF_GCSF. 1 hit.
PRINTSiPR00433. IL6GCSFMGF.
SMARTiSM00126. IL6. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00254. INTERLEUKIN_6. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning of murine interleukin-HP1: homology with human interleukin 6."
    van Snick J., Cayphas S., Szikora J.-P., Renauld J.-C., van Roost E., Boon T., Simpson R.J.
    Eur. J. Immunol. 18:193-197(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. "Genomic structure of the murine IL-6 gene. High degree conservation of potential regulatory sequences between mouse and human."
    Tanabe O., Akira S., Kamiya T., Wong G.G., Hirano T., Kishimoto T.
    J. Immunol. 141:3875-3881(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Multiple biological activities are expressed by a mouse interleukin 6 cDNA clone isolated from bone marrow stromal cells."
    Chiu C.P., Moulds C., Coffman R.L., Rennick D., Lee F.
    Proc. Natl. Acad. Sci. U.S.A. 85:7099-7103(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Cloning and sequence analysis of the cDNA for murine interleukin-6."
    Grenett H.E., Fuentes N.L., Fuller G.M.
    Nucleic Acids Res. 18:6455-6455(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Macrophage.
  6. "DNA rearrangement and constitutive expression of the interleukin 6 gene in a mouse plasmacytoma."
    Blankenstein T., Qin Z., Li W., Diamantstein T.
    J. Exp. Med. 171:965-970(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
    Strain: BALB/c.
  7. "The murine Il-6 gene maps to the proximal region of chromosome 5."
    Mock B.A., Nordan R.P., Justice M.J., Kozak C., Jenkins N.A., Copeland N.G., Clark S.C., Wong G.G., Rudikoff S.
    J. Immunol. 142:1372-1376(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 5-211.
    Strain: C57BL/6J.
  8. "Purification and NH2-terminal amino acid sequence of a T-cell-derived lymphokine with growth factor activity for B-cell hybridomas."
    van Snick J., Cayphas S., Vink A., Uyttenhove C., Coulie P.G., Rubira M.R., Simpson R.J.
    Proc. Natl. Acad. Sci. U.S.A. 83:9679-9683(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-45.
  9. "Murine hybridoma/plasmacytoma growth factor. Complete amino-acid sequence and relation to human interleukin-6."
    Simpson R.J., Moritz R.L., Rubira M.R., van Snick J.
    Eur. J. Biochem. 176:187-197(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-211.
  10. "Internal amino acid sequencing of proteins by in situ cyanogen bromide cleavage in polyacrylamide gels."
    Jahnen W., Ward L.D., Reid G.E., Moritz R.L., Simpson R.J.
    Biochem. Biophys. Res. Commun. 166:139-145(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 66-75; 78-84 AND 128-148.
  11. "Characterization of a recombinant murine interleukin-6: assignment of disulphide bonds."
    Simpson R.J., Moritz R.L., van Roost E., van Snick J.
    Biochem. Biophys. Res. Commun. 157:364-372(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS.
  12. "The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells."
    Ivanov I.I., McKenzie B.S., Zhou L., Tadokoro C.E., Lepelley A., Lafaille J.J., Cua D.J., Littman D.R.
    Cell 126:1121-1133(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TH17 DIFFERENTIATION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiIL6_MOUSE
AccessioniPrimary (citable) accession number: P08505
Secondary accession number(s): Q3UCQ0, Q8BN26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: June 24, 2015
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.