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P08492

- HN_PI3H4

UniProt

P08492 - HN_PI3H4

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Protein
Hemagglutinin-neuraminidase
Gene
HN
Organism
Human parainfluenza 3 virus (strain Wash/47885/57) (HPIV-3) (Human parainfluenza 3 virus (strain NIH 47885))
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion By similarity.
Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins By similarity.

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

pH dependencei

Optimum pH is 4.7.

GO - Molecular functioni

  1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
  2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
  3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC

GO - Biological processi

  1. viral entry into host cell Source: UniProtKB-KW
  2. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Keywords - Biological processi

Host-virus interaction, Viral attachment to host cell, Virus entry into host cell

Protein family/group databases

CAZyiGH83. Glycoside Hydrolase Family 83.

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-neuraminidase (EC:3.2.1.18)
Gene namesi
Name:HN
OrganismiHuman parainfluenza 3 virus (strain Wash/47885/57) (HPIV-3) (Human parainfluenza 3 virus (strain NIH 47885))
Taxonomic identifieri11217 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesParamyxoviridaeParamyxovirinaeRespirovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3131Intravirion Reviewed prediction
Add
BLAST
Transmembranei32 – 5221Helical; Reviewed prediction
Add
BLAST
Topological domaini53 – 572520Virion surface Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 572572Hemagglutinin-neuraminidase
PRO_0000142625Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi308 – 3081N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi351 – 3511N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi523 – 5231N-linked (GlcNAc...); by host Reviewed prediction

Keywords - PTMi

Glycoprotein

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi148 – 1503
Helixi153 – 1564
Beta strandi160 – 1623
Beta strandi164 – 1663
Beta strandi168 – 1703
Beta strandi177 – 1793
Beta strandi190 – 1989
Beta strandi203 – 21513
Beta strandi221 – 23111
Beta strandi235 – 24713
Turni249 – 2513
Beta strandi254 – 2618
Beta strandi264 – 2707
Helixi276 – 2816
Beta strandi282 – 2843
Beta strandi288 – 2936
Beta strandi299 – 3046
Helixi306 – 3083
Beta strandi309 – 3135
Beta strandi315 – 3206
Beta strandi326 – 3283
Beta strandi331 – 34212
Helixi360 – 3667
Helixi370 – 3723
Beta strandi377 – 38610
Helixi388 – 3903
Beta strandi392 – 3987
Turni401 – 4033
Beta strandi410 – 4156
Beta strandi418 – 4236
Beta strandi433 – 4386
Beta strandi446 – 4494
Beta strandi461 – 4633
Beta strandi481 – 4855
Beta strandi488 – 4969
Beta strandi498 – 5036
Beta strandi505 – 5139
Beta strandi515 – 5228
Beta strandi526 – 53813
Beta strandi541 – 55212
Turni553 – 5564
Beta strandi557 – 56610
Beta strandi569 – 5713

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4MZAX-ray1.65A/B136-572[»]
4MZEX-ray1.80A/B136-572[»]
ProteinModelPortaliP08492.
SMRiP08492. Positions 142-572.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR000665. Hemagglutn-neuramid.
IPR016285. Hemagglutn-neuramid_paramyxo.
IPR011040. Sialidases.
[Graphical view]
PfamiPF00423. HN. 1 hit.
[Graphical view]
PIRSFiPIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAMiSSF50939. SSF50939. 1 hit.

Sequencei

Sequence statusi: Complete.

P08492-1 [UniParc]FASTAAdd to Basket

« Hide

MEYWKHTNHG KDAGNELETS MATHGNKITN KITYILWTII LVLLSIVFII    50
VLINSIKSEK AHESLLQDVN NEFMEVTEKI QMASDNINDL IQSGVNTRLL 100
TIQSHVQNYI PISLTQQMSD LRKFISEITI RNDNQEVPPQ RITHDVGIKP 150
LNPDDFWRCT SGLPSLMKTP KIRLMPGPGL LAMPTTVDGC VRTPSLVIND 200
LIYAYTSNLI TRGCQDIGKS YQVLQIGIIT VNSDLVPDLN PRISHTFNIN 250
DNRKSCSLAL LNTDVYQLCS TPKVDERSDY ASSGIEDIVL DIVNHDGSIS 300
TTRFKNNNIS FDQPYAALYP SVGPGIYYKG KIIFLGYGGL EHPINENAIC 350
NTTGCPGKTQ RDCNQASHSP WFSDRRMVNS IIVVDKGLNS IPKLKVWTIS 400
MRQNYWGSEG RLLLLGNKIY IYTRSTSWHS KLQLGIIDIT DYSDIRIKWT 450
WHNVLSRPGN NECPWGHSCP DGCITGVYTD AYPLNPTGSI VSSVILDSQK 500
SRVNPVITYS TSTERVNELA IRNKTLSAGY TTTSCITHYN KGYCFHIVEI 550
NHKSLDTFQP MLFKTEIPKS CS 572
Length:572
Mass (Da):64,247
Last modified:August 1, 1988 - v1
Checksum:iBDCA6F9A41E20369
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti193 – 1931T → I in strain: Isolate ZM1; 4-GU-DANA antiviral drug resistant and increased receptor binding avidity. 1 Publication
Natural varianti216 – 2161D → N in strain: Isolate C28; 50% loss of neuraminidase activity. 1 Publication
Natural varianti567 – 5671I → V in strain: Isolate ZM1.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti135 – 1351Q → R in AAA46853. 1 Publication
Sequence conflicti146 – 1461V → A in AAA46853. 1 Publication
Sequence conflicti489 – 4891S → G in AAA46856. 1 Publication
Sequence conflicti512 – 5121S → A1 Publication
Sequence conflicti512 – 5121S → A1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M17641 mRNA. Translation: AAA46846.1.
M21649 Genomic RNA. Translation: AAA46853.1.
M20402 mRNA. Translation: AAA46856.1.
PIRiA27765. HNNZP3.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M17641 mRNA. Translation: AAA46846.1 .
M21649 Genomic RNA. Translation: AAA46853.1 .
M20402 mRNA. Translation: AAA46856.1 .
PIRi A27765. HNNZP3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4MZA X-ray 1.65 A/B 136-572 [» ]
4MZE X-ray 1.80 A/B 136-572 [» ]
ProteinModelPortali P08492.
SMRi P08492. Positions 142-572.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH83. Glycoside Hydrolase Family 83.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.120.10.10. 1 hit.
InterProi IPR000665. Hemagglutn-neuramid.
IPR016285. Hemagglutn-neuramid_paramyxo.
IPR011040. Sialidases.
[Graphical view ]
Pfami PF00423. HN. 1 hit.
[Graphical view ]
PIRSFi PIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAMi SSF50939. SSF50939. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Human parainfluenza type 3 virus hemagglutinin-neuraminidase glycoprotein: nucleotide sequence of mRNA and limited amino acid sequence of the purified protein."
    Elango N., Coligan J.E., Jambou R.C., Venkatesan S.
    J. Virol. 57:481-489(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular cloning and sequence analysis of the human parainfluenza 3 virus genes encoding the surface glycoproteins, F and HN."
    Galinski M.S., Mink M.A., Pons M.W.
    Virus Res. 8:205-215(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Nucleotide sequence of the coding and flanking regions of the human parainfluenza virus 3 hemagglutinin-neuraminidase gene: comparison with other paramyxoviruses."
    Storey D.G., Cote M.-J., Dimock K., Kang C.Y.
    Intervirology 27:69-80(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Hemagglutinin-neuraminidase of human parainfluenza 3: role of the neuraminidase in the viral life cycle."
    Huberman K., Peluso R.W., Moscona A.
    Virology 214:294-300(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT NEURAMINIDASE-DEFICIENT ASN-216.
    Strain: Isolate C28.
  5. "Mutations in human parainfluenza virus type 3 hemagglutinin-neuraminidase causing increased receptor binding activity and resistance to the transition state sialic acid analog 4-GU-DANA (Zanamivir)."
    Murrell M., Porotto M., Weber T., Greengard O., Moscona A.
    J. Virol. 77:309-317(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT 4-GU-DANA-RESISTANT ILE-193.
    Strain: Isolate ZM1.

Entry informationi

Entry nameiHN_PI3H4
AccessioniPrimary (citable) accession number: P08492
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: April 16, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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