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P08492

- HN_PI3H4

UniProt

P08492 - HN_PI3H4

Protein

Hemagglutinin-neuraminidase

Gene

HN

Organism
Human parainfluenza 3 virus (strain Wash/47885/57) (HPIV-3) (Human parainfluenza 3 virus (strain NIH 47885))
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion By similarity.By similarity
    Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins.By similarity

    Catalytic activityi

    Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

    pH dependencei

    Optimum pH is 4.7.

    GO - Molecular functioni

    1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
    2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
    3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. viral entry into host cell Source: UniProtKB-KW
    2. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hemagglutinin, Hydrolase

    Keywords - Biological processi

    Host-virus interaction, Viral attachment to host cell, Virus entry into host cell

    Protein family/group databases

    CAZyiGH83. Glycoside Hydrolase Family 83.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hemagglutinin-neuraminidase (EC:3.2.1.18)
    Gene namesi
    Name:HN
    OrganismiHuman parainfluenza 3 virus (strain Wash/47885/57) (HPIV-3) (Human parainfluenza 3 virus (strain NIH 47885))
    Taxonomic identifieri11217 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesParamyxoviridaeParamyxovirinaeRespirovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]

    Subcellular locationi

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. viral envelope Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 572572Hemagglutinin-neuraminidasePRO_0000142625Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi308 – 3081N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi351 – 3511N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi523 – 5231N-linked (GlcNAc...); by hostSequence Analysis

    Keywords - PTMi

    Glycoprotein

    Structurei

    Secondary structure

    1
    572
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi148 – 1503
    Helixi153 – 1564
    Beta strandi160 – 1623
    Beta strandi164 – 1663
    Beta strandi168 – 1703
    Beta strandi177 – 1793
    Beta strandi190 – 1989
    Beta strandi203 – 21513
    Beta strandi221 – 23111
    Beta strandi235 – 24713
    Turni249 – 2513
    Beta strandi254 – 2618
    Beta strandi264 – 2707
    Helixi276 – 2816
    Beta strandi282 – 2843
    Beta strandi288 – 2936
    Beta strandi299 – 3046
    Helixi306 – 3083
    Beta strandi309 – 3135
    Beta strandi315 – 3206
    Beta strandi326 – 3283
    Beta strandi331 – 34212
    Helixi360 – 3667
    Helixi370 – 3723
    Beta strandi377 – 38610
    Helixi388 – 3903
    Beta strandi392 – 3987
    Turni401 – 4033
    Beta strandi410 – 4156
    Beta strandi418 – 4236
    Beta strandi433 – 4386
    Beta strandi446 – 4494
    Beta strandi461 – 4633
    Beta strandi481 – 4855
    Beta strandi488 – 4969
    Beta strandi498 – 5036
    Beta strandi505 – 5139
    Beta strandi515 – 5228
    Beta strandi526 – 53813
    Beta strandi541 – 55212
    Turni553 – 5564
    Beta strandi557 – 56610
    Beta strandi569 – 5713

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4MZAX-ray1.65A/B136-572[»]
    4MZEX-ray1.80A/B136-572[»]
    ProteinModelPortaliP08492.
    SMRiP08492. Positions 142-572.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3131IntravirionSequence AnalysisAdd
    BLAST
    Topological domaini53 – 572520Virion surfaceSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei32 – 5221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di2.120.10.10. 1 hit.
    InterProiIPR000665. Hemagglutn-neuramid.
    IPR016285. Hemagglutn-neuramid_paramyxo.
    IPR011040. Sialidases.
    [Graphical view]
    PfamiPF00423. HN. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
    SUPFAMiSSF50939. SSF50939. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P08492-1 [UniParc]FASTAAdd to Basket

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    MEYWKHTNHG KDAGNELETS MATHGNKITN KITYILWTII LVLLSIVFII    50
    VLINSIKSEK AHESLLQDVN NEFMEVTEKI QMASDNINDL IQSGVNTRLL 100
    TIQSHVQNYI PISLTQQMSD LRKFISEITI RNDNQEVPPQ RITHDVGIKP 150
    LNPDDFWRCT SGLPSLMKTP KIRLMPGPGL LAMPTTVDGC VRTPSLVIND 200
    LIYAYTSNLI TRGCQDIGKS YQVLQIGIIT VNSDLVPDLN PRISHTFNIN 250
    DNRKSCSLAL LNTDVYQLCS TPKVDERSDY ASSGIEDIVL DIVNHDGSIS 300
    TTRFKNNNIS FDQPYAALYP SVGPGIYYKG KIIFLGYGGL EHPINENAIC 350
    NTTGCPGKTQ RDCNQASHSP WFSDRRMVNS IIVVDKGLNS IPKLKVWTIS 400
    MRQNYWGSEG RLLLLGNKIY IYTRSTSWHS KLQLGIIDIT DYSDIRIKWT 450
    WHNVLSRPGN NECPWGHSCP DGCITGVYTD AYPLNPTGSI VSSVILDSQK 500
    SRVNPVITYS TSTERVNELA IRNKTLSAGY TTTSCITHYN KGYCFHIVEI 550
    NHKSLDTFQP MLFKTEIPKS CS 572
    Length:572
    Mass (Da):64,247
    Last modified:August 1, 1988 - v1
    Checksum:iBDCA6F9A41E20369
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti135 – 1351Q → R in AAA46853. (PubMed:2825443)Curated
    Sequence conflicti146 – 1461V → A in AAA46853. (PubMed:2825443)Curated
    Sequence conflicti489 – 4891S → G in AAA46856. (PubMed:2822598)Curated
    Sequence conflicti512 – 5121S → A(PubMed:2825443)Curated
    Sequence conflicti512 – 5121S → A(PubMed:2822598)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti193 – 1931T → I in strain: Isolate ZM1; 4-GU-DANA antiviral drug resistant and increased receptor binding avidity. 1 Publication
    Natural varianti216 – 2161D → N in strain: Isolate C28; 50% loss of neuraminidase activity. 1 Publication
    Natural varianti567 – 5671I → V in strain: Isolate ZM1.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M17641 mRNA. Translation: AAA46846.1.
    M21649 Genomic RNA. Translation: AAA46853.1.
    M20402 mRNA. Translation: AAA46856.1.
    PIRiA27765. HNNZP3.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M17641 mRNA. Translation: AAA46846.1 .
    M21649 Genomic RNA. Translation: AAA46853.1 .
    M20402 mRNA. Translation: AAA46856.1 .
    PIRi A27765. HNNZP3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4MZA X-ray 1.65 A/B 136-572 [» ]
    4MZE X-ray 1.80 A/B 136-572 [» ]
    ProteinModelPortali P08492.
    SMRi P08492. Positions 142-572.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH83. Glycoside Hydrolase Family 83.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.120.10.10. 1 hit.
    InterProi IPR000665. Hemagglutn-neuramid.
    IPR016285. Hemagglutn-neuramid_paramyxo.
    IPR011040. Sialidases.
    [Graphical view ]
    Pfami PF00423. HN. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
    SUPFAMi SSF50939. SSF50939. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Human parainfluenza type 3 virus hemagglutinin-neuraminidase glycoprotein: nucleotide sequence of mRNA and limited amino acid sequence of the purified protein."
      Elango N., Coligan J.E., Jambou R.C., Venkatesan S.
      J. Virol. 57:481-489(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Molecular cloning and sequence analysis of the human parainfluenza 3 virus genes encoding the surface glycoproteins, F and HN."
      Galinski M.S., Mink M.A., Pons M.W.
      Virus Res. 8:205-215(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    3. "Nucleotide sequence of the coding and flanking regions of the human parainfluenza virus 3 hemagglutinin-neuraminidase gene: comparison with other paramyxoviruses."
      Storey D.G., Cote M.-J., Dimock K., Kang C.Y.
      Intervirology 27:69-80(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "Hemagglutinin-neuraminidase of human parainfluenza 3: role of the neuraminidase in the viral life cycle."
      Huberman K., Peluso R.W., Moscona A.
      Virology 214:294-300(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT NEURAMINIDASE-DEFICIENT ASN-216.
      Strain: Isolate C28.
    5. "Mutations in human parainfluenza virus type 3 hemagglutinin-neuraminidase causing increased receptor binding activity and resistance to the transition state sialic acid analog 4-GU-DANA (Zanamivir)."
      Murrell M., Porotto M., Weber T., Greengard O., Moscona A.
      J. Virol. 77:309-317(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT 4-GU-DANA-RESISTANT ILE-193.
      Strain: Isolate ZM1.

    Entry informationi

    Entry nameiHN_PI3H4
    AccessioniPrimary (citable) accession number: P08492
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3