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Protein

Alpha-amylase

Gene
N/A
Organism
Streptomyces hygroscopicus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi122 – 1221CalciumBy similarity
Metal bindingi175 – 1751CalciumBy similarity
Active sitei204 – 2041NucleophileBy similarity
Metal bindingi208 – 2081Calcium; via carbonyl oxygenBy similarity
Active sitei230 – 2301Proton donorBy similarity
Sitei292 – 2921Transition state stabilizerBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase (EC:3.2.1.1)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
OrganismiStreptomyces hygroscopicus
Taxonomic identifieri1912 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Add
BLAST
Chaini31 – 478448Alpha-amylasePRO_0000001341Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP08486.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08486-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQRSRVLGG TLAGIVAAAA ATVAPWPSQA TPPGQKTVTA TLFERKYVDV
60 70 80 90 100
AKACTDQLGP AGYGYVEVSP ASEHIQGGQW WTSYQPVSYK IAGRLGDRDA
110 120 130 140 150
FASMVSACHA AGVKVIADAV VNHMAAGSGR HHAQYTKYNY PGFYQDQTFH
160 170 180 190 200
GCRKSISDYT NRDDVQTCEL VDLADLGTGS DYVRTTIAGY LGLRSLGVDG
210 220 230 240 250
FRIDAAKHIS ATDLAAVKGK MKDPGFWVQE VIYGAGEAVR PDEYTGIGDV
260 270 280 290 300
DEFRYGTHLK SAFQSGNIAQ LKSVADGKLW QRQARTFVDN WDTERNGSTL
310 320 330 340 350
TYKDGAAYTL ANVFMLASPY GSPNVYSGYE WTDKDAAAGG STGWTDDAAK
360 370 380 390 400
REITGMVGFR NAVGSAELTN WWDNGGRPLA FARSDKGFVA LNNGDAALTQ
410 420 430 440 450
TFATSLPAGT YCDVVHAASS CDGDTVTVGD TEAQVDAAKS VALHVGATGQ
460 470
SACRQAVALH VPGQSAGSPR SSAKRVEQ
Length:478
Mass (Da):50,755
Last modified:August 1, 1988 - v1
Checksum:iFC7B0AF9C6F22F4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15540 Genomic DNA. Translation: AAA26698.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15540 Genomic DNA. Translation: AAA26698.1.

3D structure databases

ProteinModelPortaliP08486.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and characterization of the Streptomyces hygroscopicus alpha-amylase gene."
    Hoshiko S., Makabe O., Nojiri C., Katsumata K., Satoh E., Nagaoka K.
    J. Bacteriol. 169:1029-1036(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiAMY_STRHY
AccessioniPrimary (citable) accession number: P08486
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: May 11, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.