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Protein

Muscarinic acetylcholine receptor M4

Gene

Chrm4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.

GO - Molecular functioni

  • G-protein coupled acetylcholine receptor activity Source: RGD
  • guanyl-nucleotide exchange factor activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M4
Gene namesi
Name:Chrm4
Synonyms:Chrm-4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2344. Chrm4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3030ExtracellularBy similarityAdd
BLAST
Transmembranei31 – 5323Helical; Name=1By similarityAdd
BLAST
Topological domaini54 – 6714CytoplasmicBy similarityAdd
BLAST
Transmembranei68 – 8821Helical; Name=2By similarityAdd
BLAST
Topological domaini89 – 10517ExtracellularBy similarityAdd
BLAST
Transmembranei106 – 12722Helical; Name=3By similarityAdd
BLAST
Topological domaini128 – 14720CytoplasmicBy similarityAdd
BLAST
Transmembranei148 – 17023Helical; Name=4By similarityAdd
BLAST
Topological domaini171 – 19222ExtracellularBy similarityAdd
BLAST
Transmembranei193 – 21523Helical; Name=5By similarityAdd
BLAST
Topological domaini216 – 400185CytoplasmicBy similarityAdd
BLAST
Transmembranei401 – 42121Helical; Name=6By similarityAdd
BLAST
Topological domaini422 – 43514ExtracellularBy similarityAdd
BLAST
Transmembranei436 – 45520Helical; Name=7By similarityAdd
BLAST
Topological domaini456 – 47823CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • asymmetric synapse Source: RGD
  • axon terminus Source: RGD
  • cell junction Source: UniProtKB-KW
  • cytoplasm Source: RGD
  • integral component of plasma membrane Source: InterPro
  • membrane Source: RGD
  • neuronal cell body Source: RGD
  • postsynaptic density Source: RGD
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1907609.
GuidetoPHARMACOLOGYi16.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 478478Muscarinic acetylcholine receptor M4PRO_0000069039Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi8 – 81N-linked (GlcNAc...)Sequence analysis
Glycosylationi13 – 131N-linked (GlcNAc...)Sequence analysis
Disulfide bondi104 ↔ 184PROSITE-ProRule annotation
Modified residuei458 – 4581PhosphothreonineSequence analysis
Modified residuei462 – 4621PhosphothreonineSequence analysis
Modified residuei476 – 4761PhosphothreonineSequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP08485.

PTM databases

iPTMnetiP08485.
PhosphoSiteiP08485.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Camk2aP1127510EBI-7946147,EBI-2640645

Protein-protein interaction databases

IntActiP08485. 1 interaction.
MINTiMINT-7984475.
STRINGi10116.ENSRNOP00000023586.

Chemistry

BindingDBiP08485.

Structurei

3D structure databases

ProteinModelPortaliP08485.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP08485.
KOiK04132.
PhylomeDBiP08485.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001432. Musac_Ach_M4_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF60. PTHR24249:SF60. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00541. MUSCRINICM4R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08485-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MXNFTPVNGS SANQSVRLVT AAHNHLETVE MVFIATVTGS LSLVTVVGNI
60 70 80 90 100
LVMLSIKVNR QLQTVNNYFL FSLGCADLII GAFSMNLYTL YIIKGYWPLG
110 120 130 140 150
AVVCDLWLAL DYVVSNASVM NLLIISFDRY FCVTKPLTYP ARRTTKMAGL
160 170 180 190 200
MIAAAWVLSF VLWAPAILFW QFVVGKRTVP DNQCFIQFLS NPAVTFGTAI
210 220 230 240 250
AAFYLPVVIM TVLYIHISLA SRSRVHKHRP EGPKEKKAKT LAFLKSPLMK
260 270 280 290 300
PSIKKPPPGG ASREELRNGK LEEAPPPALP PPPRPVPDKD TSNESSSGSA
310 320 330 340 350
TQNTKERPPT ELSTAEATTP ALPAPTLQPR TLNPASKWSK IQIVTKQTGN
360 370 380 390 400
ECVTAIEIVP ATPAGMRPAA NVARKFASIA RNQVRKKRQM AARERKVTRT
410 420 430 440 450
IFAILLAFIL TWTPYNVMVL VNTFCQSCIP ERVWSIGYWL CYVNSTINPA
460 470
CYALCNATFK KTFRHLLLCQ YRNIGTAR
Length:478
Mass (Da):52,921
Last modified:August 1, 1988 - v1
Checksum:i2551FF5F77B1A56C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16409 mRNA. Translation: AAA40663.1. Sequence problems.
PIRiC94518. C29514.
RefSeqiNP_113735.1. NM_031547.1.
UniGeneiRn.10676.

Genome annotation databases

GeneIDi25111.
KEGGirno:25111.
UCSCiRGD:2344. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16409 mRNA. Translation: AAA40663.1. Sequence problems.
PIRiC94518. C29514.
RefSeqiNP_113735.1. NM_031547.1.
UniGeneiRn.10676.

3D structure databases

ProteinModelPortaliP08485.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP08485. 1 interaction.
MINTiMINT-7984475.
STRINGi10116.ENSRNOP00000023586.

Chemistry

BindingDBiP08485.
ChEMBLiCHEMBL1907609.
GuidetoPHARMACOLOGYi16.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP08485.
PhosphoSiteiP08485.

Proteomic databases

PaxDbiP08485.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25111.
KEGGirno:25111.
UCSCiRGD:2344. rat.

Organism-specific databases

CTDi1132.
RGDi2344. Chrm4.

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP08485.
KOiK04132.
PhylomeDBiP08485.

Miscellaneous databases

PROiP08485.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001432. Musac_Ach_M4_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF60. PTHR24249:SF60. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00541. MUSCRINICM4R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACM4_RAT
AccessioniPrimary (citable) accession number: P08485
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: July 6, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.