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Protein

Muscarinic acetylcholine receptor M3

Gene

Chrm3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.3 Publications

GO - Molecular functioni

  • acetylcholine binding Source: UniProtKB
  • drug binding Source: RGD
  • G-protein coupled acetylcholine receptor activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-RNO-390648. Muscarinic acetylcholine receptors.
R-RNO-416476. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M3
Gene namesi
Name:Chrm3
Synonyms:Chrm-3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi2343. Chrm3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 66Extracellular1 PublicationAdd BLAST66
Transmembranei67 – 90Helical; Name=1Add BLAST24
Topological domaini91 – 103Cytoplasmic1 PublicationAdd BLAST13
Transmembranei104 – 129Helical; Name=2Add BLAST26
Topological domaini130 – 141Extracellular1 PublicationAdd BLAST12
Transmembranei142 – 163Helical; Name=3Add BLAST22
Topological domaini164 – 183Cytoplasmic1 PublicationAdd BLAST20
Transmembranei184 – 205Helical; Name=4Add BLAST22
Topological domaini206 – 228Extracellular1 PublicationAdd BLAST23
Transmembranei229 – 251Helical; Name=5Add BLAST23
Topological domaini252 – 490Cytoplasmic1 PublicationAdd BLAST239
Transmembranei491 – 513Helical; Name=6Add BLAST23
Topological domaini514 – 525Extracellular1 PublicationAdd BLAST12
Transmembranei526 – 545Helical; Name=7Add BLAST20
Topological domaini546 – 589Cytoplasmic1 PublicationAdd BLAST44

GO - Cellular componenti

  • asymmetric synapse Source: RGD
  • axon terminus Source: RGD
  • basolateral plasma membrane Source: UniProtKB-SubCell
  • cell junction Source: UniProtKB-KW
  • dendrite Source: RGD
  • integral component of plasma membrane Source: UniProtKB
  • plasma membrane Source: RGD
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi148Y → A: Decreased affinity for acetylcholine. 1 Publication1
Mutagenesisi231T → A: Decreased affinity for acetylcholine. 1 Publication1
Mutagenesisi234T → A: Strongly decreased affinity for acetylcholine. 2 Publications1
Mutagenesisi506Y → F: Decreased affinity for acetylcholine. 2 Publications1
Mutagenesisi529Y → F: Decreased affinity for acetylcholine. 1 Publication1
Mutagenesisi533Y → F: Decreased affinity for acetylcholine. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL320.
GuidetoPHARMACOLOGYi15.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000690341 – 589Muscarinic acetylcholine receptor M3Add BLAST589

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi6N-linked (GlcNAc...)Sequence analysis1
Glycosylationi15N-linked (GlcNAc...)Sequence analysis1
Glycosylationi41N-linked (GlcNAc...)Sequence analysis1
Glycosylationi48N-linked (GlcNAc...)Sequence analysis1
Glycosylationi52N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi140 ↔ 220PROSITE-ProRule annotation1 Publication
Modified residuei384PhosphoserineBy similarity1
Disulfide bondi516 ↔ 519PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP08483.
PRIDEiP08483.

PTM databases

PhosphoSitePlusiP08483.

Expressioni

Gene expression databases

BgeeiENSRNOG00000049410.
GenevisibleiP08483. RN.

Interactioni

Subunit structurei

Homodimer; the dimers can form tetramers. Interacts with NALCN.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Camk2aP112752EBI-7946407,EBI-2640645

Protein-protein interaction databases

IntActiP08483. 1 interactor.
STRINGi10116.ENSRNOP00000067435.

Chemistry databases

BindingDBiP08483.

Structurei

Secondary structure

1589
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi65 – 94Combined sources30
Helixi96 – 98Combined sources3
Helixi102 – 118Combined sources17
Helixi120 – 129Combined sources10
Beta strandi130 – 132Combined sources3
Helixi137 – 170Combined sources34
Beta strandi173 – 175Combined sources3
Turni176 – 178Combined sources3
Helixi181 – 210Combined sources30
Helixi223 – 225Combined sources3
Helixi228 – 238Combined sources11
Helixi240 – 253Combined sources14
Helixi255 – 257Combined sources3
Helixi483 – 502Combined sources20
Helixi504 – 513Combined sources10
Beta strandi517 – 519Combined sources3
Helixi522 – 533Combined sources12
Helixi535 – 544Combined sources10
Helixi548 – 556Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AMKmodel-A56-560[»]
4DAJX-ray3.40A/B/C/D57-259[»]
A/B/C/D482-589[»]
4U14X-ray3.57A57-259[»]
A482-563[»]
4U15X-ray2.80A/B57-259[»]
A/B482-563[»]
4U16X-ray3.70A/B57-259[»]
A/B482-563[»]
ProteinModelPortaliP08483.
SMRiP08483.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni147 – 151Agonist bindingCurated5
Regioni506 – 529Agonist bindingCuratedAdd BLAST24

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi274 – 280Basolateral sorting signalBy similarity7

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP08483.
KOiK04131.
OMAiKSMDDGG.
OrthoDBiEOG091G06VI.
PhylomeDBiP08483.
TreeFamiTF320495.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001183. Musac_Ach_M3_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF61. PTHR24249:SF61. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00540. MUSCRINICM3R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08483-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLHSNSTTS PLFPNISSSW VHSPSEAGLP LGTVTQLGSY NISQETGNFS
60 70 80 90 100
SNDTSSDPLG GHTIWQVVFI AFLTGFLALV TIIGNILVIV AFKVNKQLKT
110 120 130 140 150
VNNYFLLSLA CADLIIGVIS MNLFTTYIIM NRWALGNLAC DLWLSIDYVA
160 170 180 190 200
SNASVMNLLV ISFDRYFSIT RPLTYRAKRT TKRAGVMIGL AWVISFVLWA
210 220 230 240 250
PAILFWQYFV GKRTVPPGEC FIQFLSEPTI TFGTAIAAFY MPVTIMTILY
260 270 280 290 300
WRIYKETEKR TKELAGLQAS GTEAEAENFV HPTGSSRSCS SYELQQQGVK
310 320 330 340 350
RSSRRKYGRC HFWFTTKSWK PSAEQMDQDH SSSDSWNNND AAASLENSAS
360 370 380 390 400
SDEEDIGSET RAIYSIVLKL PGHSSILNST KLPSSDNLQV SNEDLGTVDV
410 420 430 440 450
ERNAHKLQAQ KSMGDGDNCQ KDFTKLPIQL ESAVDTGKTS DTNSSADKTT
460 470 480 490 500
ATLPLSFKEA TLAKRFALKT RSQITKRKRM SLIKEKKAAQ TLSAILLAFI
510 520 530 540 550
ITWTPYNIMV LVNTFCDSCI PKTYWNLGYW LCYINSTVNP VCYALCNKTF
560 570 580
RTTFKTLLLC QCDKRKRRKQ QYQQRQSVIF HKRVPEQAL
Length:589
Mass (Da):66,066
Last modified:November 1, 1988 - v1
Checksum:i9A5EF2FA653C830A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti184A → R in AAA40661 (PubMed:3037705).Curated1
Sequence conflicti184A → R in AAA40662 (PubMed:3037705).Curated1
Sequence conflicti184A → R in BAA36839 (PubMed:9972520).Curated1
Sequence conflicti516C → R in AAA40659 (PubMed:3120722).Curated1
Sequence conflicti556T → M in AAA40659 (PubMed:3120722).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16407 mRNA. Translation: AAA40661.1.
M16408 Genomic DNA. Translation: AAA40662.1.
M18088 mRNA. Translation: AAA40659.1.
M62826 Genomic DNA. Translation: AAA41553.1.
AB017656 mRNA. Translation: BAA36839.1.
PIRiA29476.
B94518. B29514.
RefSeqiNP_036659.2. NM_012527.2.
XP_017455938.1. XM_017600449.1.
XP_017455939.1. XM_017600450.1.
XP_017455940.1. XM_017600451.1.
XP_017455941.1. XM_017600452.1.
UniGeneiRn.87735.

Genome annotation databases

EnsembliENSRNOT00000075651; ENSRNOP00000067435; ENSRNOG00000049410.
GeneIDi24260.
KEGGirno:24260.
UCSCiRGD:2343. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16407 mRNA. Translation: AAA40661.1.
M16408 Genomic DNA. Translation: AAA40662.1.
M18088 mRNA. Translation: AAA40659.1.
M62826 Genomic DNA. Translation: AAA41553.1.
AB017656 mRNA. Translation: BAA36839.1.
PIRiA29476.
B94518. B29514.
RefSeqiNP_036659.2. NM_012527.2.
XP_017455938.1. XM_017600449.1.
XP_017455939.1. XM_017600450.1.
XP_017455940.1. XM_017600451.1.
XP_017455941.1. XM_017600452.1.
UniGeneiRn.87735.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AMKmodel-A56-560[»]
4DAJX-ray3.40A/B/C/D57-259[»]
A/B/C/D482-589[»]
4U14X-ray3.57A57-259[»]
A482-563[»]
4U15X-ray2.80A/B57-259[»]
A/B482-563[»]
4U16X-ray3.70A/B57-259[»]
A/B482-563[»]
ProteinModelPortaliP08483.
SMRiP08483.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP08483. 1 interactor.
STRINGi10116.ENSRNOP00000067435.

Chemistry databases

BindingDBiP08483.
ChEMBLiCHEMBL320.
GuidetoPHARMACOLOGYi15.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSitePlusiP08483.

Proteomic databases

PaxDbiP08483.
PRIDEiP08483.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000075651; ENSRNOP00000067435; ENSRNOG00000049410.
GeneIDi24260.
KEGGirno:24260.
UCSCiRGD:2343. rat.

Organism-specific databases

CTDi1131.
RGDi2343. Chrm3.

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP08483.
KOiK04131.
OMAiKSMDDGG.
OrthoDBiEOG091G06VI.
PhylomeDBiP08483.
TreeFamiTF320495.

Enzyme and pathway databases

ReactomeiR-RNO-390648. Muscarinic acetylcholine receptors.
R-RNO-416476. G alpha (q) signalling events.

Miscellaneous databases

PROiP08483.

Gene expression databases

BgeeiENSRNOG00000049410.
GenevisibleiP08483. RN.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001183. Musac_Ach_M3_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF61. PTHR24249:SF61. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00540. MUSCRINICM3R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACM3_RAT
AccessioniPrimary (citable) accession number: P08483
Secondary accession number(s): Q9QWK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1988
Last modified: November 30, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.