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P08482

- ACM1_RAT

UniProt

P08482 - ACM1_RAT

Protein

Muscarinic acetylcholine receptor M1

Gene

Chrm1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei105 – 1051Antagonist

    GO - Molecular functioni

    1. drug binding Source: RGD
    2. G-protein coupled acetylcholine receptor activity Source: RGD

    GO - Biological processi

    1. cognition Source: InterPro
    2. G-protein coupled acetylcholine receptor signaling pathway Source: RGD
    3. neuromuscular synaptic transmission Source: RGD
    4. positive regulation of intracellular protein transport Source: RGD
    5. positive regulation of ion transport Source: Ensembl
    6. regulation of locomotion Source: Ensembl
    7. saliva secretion Source: InterPro

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Muscarinic acetylcholine receptor M1
    Gene namesi
    Name:Chrm1
    Synonyms:Chrm-1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi2342. Chrm1.

    Subcellular locationi

    GO - Cellular componenti

    1. asymmetric synapse Source: RGD
    2. axon terminus Source: RGD
    3. cell junction Source: UniProtKB-KW
    4. dendrite Source: RGD
    5. integral component of plasma membrane Source: InterPro
    6. plasma membrane Source: RGD
    7. postsynaptic density Source: RGD
    8. postsynaptic membrane Source: UniProtKB-SubCell
    9. synapse Source: RGD

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 460460Muscarinic acetylcholine receptor M1PRO_0000069019Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi2 – 21N-linked (GlcNAc...)Curated
    Glycosylationi12 – 121N-linked (GlcNAc...)Curated
    Disulfide bondi98 ↔ 178Curated
    Modified residuei428 – 4281PhosphothreonineSequence Analysis
    Modified residuei451 – 4511PhosphoserineSequence Analysis
    Modified residuei455 – 4551PhosphothreonineSequence Analysis
    Modified residuei457 – 4571PhosphoserineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiP08482.

    PTM databases

    PhosphoSiteiP08482.

    Expressioni

    Gene expression databases

    GenevestigatoriP08482.

    Interactioni

    Subunit structurei

    Interacts with GPRASP2.By similarity

    Protein-protein interaction databases

    BioGridi247270. 1 interaction.
    STRINGi10116.ENSRNOP00000024785.

    Structurei

    3D structure databases

    ProteinModelPortaliP08482.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2424ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini48 – 6114CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini83 – 9917ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini122 – 14120CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini165 – 18622ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini210 – 366157CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini388 – 40114ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini422 – 46039CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei25 – 4723Helical; Name=1By similarityAdd
    BLAST
    Transmembranei62 – 8221Helical; Name=2By similarityAdd
    BLAST
    Transmembranei100 – 12122Helical; Name=3By similarityAdd
    BLAST
    Transmembranei142 – 16423Helical; Name=4By similarityAdd
    BLAST
    Transmembranei187 – 20923Helical; Name=5By similarityAdd
    BLAST
    Transmembranei367 – 38721Helical; Name=6By similarityAdd
    BLAST
    Transmembranei402 – 42120Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM1 sub-subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG250863.
    GeneTreeiENSGT00690000101881.
    HOGENOMiHOG000231484.
    HOVERGENiHBG105720.
    InParanoidiP08482.
    KOiK04129.
    OMAiRCCRTPR.
    OrthoDBiEOG7V49Z7.
    PhylomeDBiP08482.
    TreeFamiTF320495.

    Family and domain databases

    Gene3Di1.20.1070.10. 2 hits.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR002228. Musac_Ach_M1_rcpt.
    IPR000995. Musac_Ach_rcpt.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR00243. MUSCARINICR.
    PR00538. MUSCRINICM1R.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P08482-1 [UniParc]FASTAAdd to Basket

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    MNTSVPPAVS PNITVLAPGK GPWQVAFIGI TTGLLSLATV TGNLLVLISF    50
    KVNTELKTVN NYFLLSLACA DLIIGTFSMN LYTTYLLMGH WALGTLACDL 100
    WLALDYVASN ASVMNLLLIS FDRYFSVTRP LSYRAKRTPR RAALMIGLAW 150
    LVSFVLWAPA ILFWQYLVGE RTVLAGQCYI QFLSQPIITF GTAMAAFYLP 200
    VTVMCTLYWR IYRETENRAR ELAALQGSET PGKGGGSSSS SERSQPGAEG 250
    SPESPPGRCC RCCRAPRLLQ AYSWKEEEEE DEGSMESLTS SEGEEPGSEV 300
    VIKMPMVDSE AQAPTKQPPK SSPNTVKRPT KKGRDRGGKG QKPRGKEQLA 350
    KRKTFSLVKE KKAARTLSAI LLAFILTWTP YNIMVLVSTF CKDCVPETLW 400
    ELGYWLCYVN STVNPMCYAL CNKAFRDTFR LLLLCRWDKR RWRKIPKRPG 450
    SVHRTPSRQC 460
    Length:460
    Mass (Da):51,369
    Last modified:August 1, 1988 - v1
    Checksum:i527573ED8FF7C317
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16406 Genomic DNA. Translation: AAA40660.1.
    S73971 Genomic DNA. Translation: AAB20705.1.
    PIRiA94518. A29514.
    RefSeqiNP_542951.1. NM_080773.1.
    XP_006231036.1. XM_006230974.1.
    UniGeneiRn.119395.

    Genome annotation databases

    EnsembliENSRNOT00000024785; ENSRNOP00000024785; ENSRNOG00000018385.
    GeneIDi25229.
    KEGGirno:25229.
    UCSCiRGD:2342. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16406 Genomic DNA. Translation: AAA40660.1 .
    S73971 Genomic DNA. Translation: AAB20705.1 .
    PIRi A94518. A29514.
    RefSeqi NP_542951.1. NM_080773.1.
    XP_006231036.1. XM_006230974.1.
    UniGenei Rn.119395.

    3D structure databases

    ProteinModelPortali P08482.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 247270. 1 interaction.
    STRINGi 10116.ENSRNOP00000024785.

    Chemistry

    BindingDBi P08482.
    ChEMBLi CHEMBL1907609.
    GuidetoPHARMACOLOGYi 13.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P08482.

    Proteomic databases

    PRIDEi P08482.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000024785 ; ENSRNOP00000024785 ; ENSRNOG00000018385 .
    GeneIDi 25229.
    KEGGi rno:25229.
    UCSCi RGD:2342. rat.

    Organism-specific databases

    CTDi 1128.
    RGDi 2342. Chrm1.

    Phylogenomic databases

    eggNOGi NOG250863.
    GeneTreei ENSGT00690000101881.
    HOGENOMi HOG000231484.
    HOVERGENi HBG105720.
    InParanoidi P08482.
    KOi K04129.
    OMAi RCCRTPR.
    OrthoDBi EOG7V49Z7.
    PhylomeDBi P08482.
    TreeFami TF320495.

    Miscellaneous databases

    NextBioi 605783.
    PROi P08482.

    Gene expression databases

    Genevestigatori P08482.

    Family and domain databases

    Gene3Di 1.20.1070.10. 2 hits.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR002228. Musac_Ach_M1_rcpt.
    IPR000995. Musac_Ach_rcpt.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR00243. MUSCARINICR.
    PR00538. MUSCRINICM1R.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a family of muscarinic acetylcholine receptor genes."
      Bonner T.I., Buckley N.J., Young A.C., Brann M.R.
      Science 237:527-532(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The molecular properties of the M1 muscarinic receptor and its regulation of cytosolic calcium in a eukaryotic gene expression system."
      Lai J., Smith T.L., Mei L., Ikeda M., Fujiwara Y., Gomez J., Halonen M., Roeske W.R., Yamamura H.I.
      Adv. Exp. Med. Biol. 287:313-330(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Muscarinic acetylcholine receptors. Peptide sequencing identifies residues involved in antagonist binding and disulfide bond formation."
      Kurtenbach E., Curtis C.A.M., Pedder E.K., Aitken A., Harris A.C.M., Hulme E.C.
      J. Biol. Chem. 265:13702-13708(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 63-124.
    4. "Site-directed mutagenesis of the rat m1 muscarinic acetylcholine receptor. Role of conserved cysteines in receptor function."
      Savarese T.M., Wang C.-D., Fraser C.M.
      J. Biol. Chem. 267:11439-11448(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF CYSTEINE RESIDUES.

    Entry informationi

    Entry nameiACM1_RAT
    AccessioniPrimary (citable) accession number: P08482
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3