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P08482

- ACM1_RAT

UniProt

P08482 - ACM1_RAT

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Protein

Muscarinic acetylcholine receptor M1

Gene

Chrm1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei105 – 1051Antagonist

GO - Molecular functioni

  1. drug binding Source: RGD
  2. G-protein coupled acetylcholine receptor activity Source: RGD

GO - Biological processi

  1. cognition Source: InterPro
  2. G-protein coupled acetylcholine receptor signaling pathway Source: RGD
  3. neuromuscular synaptic transmission Source: RGD
  4. positive regulation of intracellular protein transport Source: RGD
  5. positive regulation of ion transport Source: Ensembl
  6. regulation of locomotion Source: Ensembl
  7. saliva secretion Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_241250. Muscarinic acetylcholine receptors.
REACT_263374. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M1
Gene namesi
Name:Chrm1
Synonyms:Chrm-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi2342. Chrm1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424ExtracellularSequence AnalysisAdd
BLAST
Transmembranei25 – 4723Helical; Name=1By similarityAdd
BLAST
Topological domaini48 – 6114CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei62 – 8221Helical; Name=2By similarityAdd
BLAST
Topological domaini83 – 9917ExtracellularSequence AnalysisAdd
BLAST
Transmembranei100 – 12122Helical; Name=3By similarityAdd
BLAST
Topological domaini122 – 14120CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei142 – 16423Helical; Name=4By similarityAdd
BLAST
Topological domaini165 – 18622ExtracellularSequence AnalysisAdd
BLAST
Transmembranei187 – 20923Helical; Name=5By similarityAdd
BLAST
Topological domaini210 – 366157CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei367 – 38721Helical; Name=6By similarityAdd
BLAST
Topological domaini388 – 40114ExtracellularSequence AnalysisAdd
BLAST
Transmembranei402 – 42120Helical; Name=7By similarityAdd
BLAST
Topological domaini422 – 46039CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. asymmetric synapse Source: RGD
  2. axon terminus Source: RGD
  3. cell junction Source: UniProtKB-KW
  4. dendrite Source: RGD
  5. integral component of plasma membrane Source: InterPro
  6. plasma membrane Source: RGD
  7. postsynaptic density Source: RGD
  8. postsynaptic membrane Source: UniProtKB-KW
  9. synapse Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 460460Muscarinic acetylcholine receptor M1PRO_0000069019Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi2 – 21N-linked (GlcNAc...)Curated
Glycosylationi12 – 121N-linked (GlcNAc...)Curated
Disulfide bondi98 ↔ 178Curated
Modified residuei428 – 4281PhosphothreonineSequence Analysis
Modified residuei451 – 4511PhosphoserineSequence Analysis
Modified residuei455 – 4551PhosphothreonineSequence Analysis
Modified residuei457 – 4571PhosphoserineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiP08482.

PTM databases

PhosphoSiteiP08482.

Expressioni

Gene expression databases

GenevestigatoriP08482.

Interactioni

Subunit structurei

Interacts with GPRASP2.By similarity

Protein-protein interaction databases

BioGridi247270. 1 interaction.
STRINGi10116.ENSRNOP00000024785.

Structurei

3D structure databases

ProteinModelPortaliP08482.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM1 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG250863.
GeneTreeiENSGT00770000120457.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP08482.
KOiK04129.
OMAiRCCRTPR.
OrthoDBiEOG7V49Z7.
PhylomeDBiP08482.
TreeFamiTF320495.

Family and domain databases

Gene3Di1.20.1070.10. 2 hits.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002228. Musac_Ach_M1_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00538. MUSCRINICM1R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08482-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNTSVPPAVS PNITVLAPGK GPWQVAFIGI TTGLLSLATV TGNLLVLISF
60 70 80 90 100
KVNTELKTVN NYFLLSLACA DLIIGTFSMN LYTTYLLMGH WALGTLACDL
110 120 130 140 150
WLALDYVASN ASVMNLLLIS FDRYFSVTRP LSYRAKRTPR RAALMIGLAW
160 170 180 190 200
LVSFVLWAPA ILFWQYLVGE RTVLAGQCYI QFLSQPIITF GTAMAAFYLP
210 220 230 240 250
VTVMCTLYWR IYRETENRAR ELAALQGSET PGKGGGSSSS SERSQPGAEG
260 270 280 290 300
SPESPPGRCC RCCRAPRLLQ AYSWKEEEEE DEGSMESLTS SEGEEPGSEV
310 320 330 340 350
VIKMPMVDSE AQAPTKQPPK SSPNTVKRPT KKGRDRGGKG QKPRGKEQLA
360 370 380 390 400
KRKTFSLVKE KKAARTLSAI LLAFILTWTP YNIMVLVSTF CKDCVPETLW
410 420 430 440 450
ELGYWLCYVN STVNPMCYAL CNKAFRDTFR LLLLCRWDKR RWRKIPKRPG
460
SVHRTPSRQC
Length:460
Mass (Da):51,369
Last modified:August 1, 1988 - v1
Checksum:i527573ED8FF7C317
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16406 Genomic DNA. Translation: AAA40660.1.
S73971 Genomic DNA. Translation: AAB20705.1.
PIRiA94518. A29514.
RefSeqiNP_542951.1. NM_080773.1.
XP_006231036.1. XM_006230974.2.
XP_008758437.1. XM_008760215.1.
UniGeneiRn.119395.

Genome annotation databases

EnsembliENSRNOT00000024785; ENSRNOP00000024785; ENSRNOG00000018385.
GeneIDi25229.
KEGGirno:25229.
UCSCiRGD:2342. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16406 Genomic DNA. Translation: AAA40660.1 .
S73971 Genomic DNA. Translation: AAB20705.1 .
PIRi A94518. A29514.
RefSeqi NP_542951.1. NM_080773.1.
XP_006231036.1. XM_006230974.2.
XP_008758437.1. XM_008760215.1.
UniGenei Rn.119395.

3D structure databases

ProteinModelPortali P08482.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 247270. 1 interaction.
STRINGi 10116.ENSRNOP00000024785.

Chemistry

BindingDBi P08482.
ChEMBLi CHEMBL1907609.
GuidetoPHARMACOLOGYi 13.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P08482.

Proteomic databases

PRIDEi P08482.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000024785 ; ENSRNOP00000024785 ; ENSRNOG00000018385 .
GeneIDi 25229.
KEGGi rno:25229.
UCSCi RGD:2342. rat.

Organism-specific databases

CTDi 1128.
RGDi 2342. Chrm1.

Phylogenomic databases

eggNOGi NOG250863.
GeneTreei ENSGT00770000120457.
HOGENOMi HOG000231484.
HOVERGENi HBG105720.
InParanoidi P08482.
KOi K04129.
OMAi RCCRTPR.
OrthoDBi EOG7V49Z7.
PhylomeDBi P08482.
TreeFami TF320495.

Enzyme and pathway databases

Reactomei REACT_241250. Muscarinic acetylcholine receptors.
REACT_263374. G alpha (q) signalling events.

Miscellaneous databases

NextBioi 605783.
PROi P08482.

Gene expression databases

Genevestigatori P08482.

Family and domain databases

Gene3Di 1.20.1070.10. 2 hits.
InterProi IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002228. Musac_Ach_M1_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view ]
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00538. MUSCRINICM1R.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of a family of muscarinic acetylcholine receptor genes."
    Bonner T.I., Buckley N.J., Young A.C., Brann M.R.
    Science 237:527-532(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The molecular properties of the M1 muscarinic receptor and its regulation of cytosolic calcium in a eukaryotic gene expression system."
    Lai J., Smith T.L., Mei L., Ikeda M., Fujiwara Y., Gomez J., Halonen M., Roeske W.R., Yamamura H.I.
    Adv. Exp. Med. Biol. 287:313-330(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Muscarinic acetylcholine receptors. Peptide sequencing identifies residues involved in antagonist binding and disulfide bond formation."
    Kurtenbach E., Curtis C.A.M., Pedder E.K., Aitken A., Harris A.C.M., Hulme E.C.
    J. Biol. Chem. 265:13702-13708(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 63-124.
  4. "Site-directed mutagenesis of the rat m1 muscarinic acetylcholine receptor. Role of conserved cysteines in receptor function."
    Savarese T.M., Wang C.-D., Fraser C.M.
    J. Biol. Chem. 267:11439-11448(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF CYSTEINE RESIDUES.

Entry informationi

Entry nameiACM1_RAT
AccessioniPrimary (citable) accession number: P08482
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 26, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3