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P08482 (ACM1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Muscarinic acetylcholine receptor M1
Gene names
Name:Chrm1
Synonyms:Chrm-1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length460 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

Subunit structure

Interacts with GPRASP2 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM1 sub-subfamily.

Ontologies

Keywords
   Cellular componentCell junction
Cell membrane
Membrane
Postsynaptic cell membrane
Synapse
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled acetylcholine receptor signaling pathway

Inferred from direct assay PubMed 12534975. Source: RGD

cognition

Inferred from electronic annotation. Source: InterPro

neuromuscular synaptic transmission

Inferred from direct assay PubMed 12534975. Source: RGD

positive regulation of intracellular protein transport

Inferred from direct assay PubMed 17908240. Source: RGD

positive regulation of ion transport

Inferred from electronic annotation. Source: Ensembl

regulation of locomotion

Inferred from electronic annotation. Source: Ensembl

saliva secretion

Inferred from electronic annotation. Source: InterPro

   Cellular_componentasymmetric synapse

Inferred from direct assay PubMed 8182478. Source: RGD

axon terminus

Inferred from direct assay PubMed 8182478. Source: RGD

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

dendrite

Inferred from direct assay PubMed 8182478. Source: RGD

integral component of plasma membrane

Inferred from electronic annotation. Source: InterPro

plasma membrane

Inferred from direct assay PubMed 8320877. Source: RGD

postsynaptic density

Inferred from direct assay PubMed 8182478. Source: RGD

postsynaptic membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

synapse

Traceable author statement PubMed 12534975. Source: RGD

   Molecular_functionG-protein coupled acetylcholine receptor activity

Inferred from direct assay PubMed 12534975. Source: RGD

drug binding

Inferred from direct assay PubMed 8320877. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 460460Muscarinic acetylcholine receptor M1
PRO_0000069019

Regions

Topological domain1 – 2424Extracellular Potential
Transmembrane25 – 4723Helical; Name=1; By similarity
Topological domain48 – 6114Cytoplasmic Potential
Transmembrane62 – 8221Helical; Name=2; By similarity
Topological domain83 – 9917Extracellular Potential
Transmembrane100 – 12122Helical; Name=3; By similarity
Topological domain122 – 14120Cytoplasmic Potential
Transmembrane142 – 16423Helical; Name=4; By similarity
Topological domain165 – 18622Extracellular Potential
Transmembrane187 – 20923Helical; Name=5; By similarity
Topological domain210 – 366157Cytoplasmic Potential
Transmembrane367 – 38721Helical; Name=6; By similarity
Topological domain388 – 40114Extracellular Potential
Transmembrane402 – 42120Helical; Name=7; By similarity
Topological domain422 – 46039Cytoplasmic Potential

Sites

Binding site1051Antagonist

Amino acid modifications

Modified residue4281Phosphothreonine Potential
Modified residue4511Phosphoserine Potential
Modified residue4551Phosphothreonine Potential
Modified residue4571Phosphoserine Potential
Glycosylation21N-linked (GlcNAc...) Probable
Glycosylation121N-linked (GlcNAc...) Probable
Disulfide bond98 ↔ 178 Probable

Sequences

Sequence LengthMass (Da)Tools
P08482 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: 527573ED8FF7C317

FASTA46051,369
        10         20         30         40         50         60 
MNTSVPPAVS PNITVLAPGK GPWQVAFIGI TTGLLSLATV TGNLLVLISF KVNTELKTVN 

        70         80         90        100        110        120 
NYFLLSLACA DLIIGTFSMN LYTTYLLMGH WALGTLACDL WLALDYVASN ASVMNLLLIS 

       130        140        150        160        170        180 
FDRYFSVTRP LSYRAKRTPR RAALMIGLAW LVSFVLWAPA ILFWQYLVGE RTVLAGQCYI 

       190        200        210        220        230        240 
QFLSQPIITF GTAMAAFYLP VTVMCTLYWR IYRETENRAR ELAALQGSET PGKGGGSSSS 

       250        260        270        280        290        300 
SERSQPGAEG SPESPPGRCC RCCRAPRLLQ AYSWKEEEEE DEGSMESLTS SEGEEPGSEV 

       310        320        330        340        350        360 
VIKMPMVDSE AQAPTKQPPK SSPNTVKRPT KKGRDRGGKG QKPRGKEQLA KRKTFSLVKE 

       370        380        390        400        410        420 
KKAARTLSAI LLAFILTWTP YNIMVLVSTF CKDCVPETLW ELGYWLCYVN STVNPMCYAL 

       430        440        450        460 
CNKAFRDTFR LLLLCRWDKR RWRKIPKRPG SVHRTPSRQC 

« Hide

References

[1]"Identification of a family of muscarinic acetylcholine receptor genes."
Bonner T.I., Buckley N.J., Young A.C., Brann M.R.
Science 237:527-532(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The molecular properties of the M1 muscarinic receptor and its regulation of cytosolic calcium in a eukaryotic gene expression system."
Lai J., Smith T.L., Mei L., Ikeda M., Fujiwara Y., Gomez J., Halonen M., Roeske W.R., Yamamura H.I.
Adv. Exp. Med. Biol. 287:313-330(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Muscarinic acetylcholine receptors. Peptide sequencing identifies residues involved in antagonist binding and disulfide bond formation."
Kurtenbach E., Curtis C.A.M., Pedder E.K., Aitken A., Harris A.C.M., Hulme E.C.
J. Biol. Chem. 265:13702-13708(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 63-124.
[4]"Site-directed mutagenesis of the rat m1 muscarinic acetylcholine receptor. Role of conserved cysteines in receptor function."
Savarese T.M., Wang C.-D., Fraser C.M.
J. Biol. Chem. 267:11439-11448(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF CYSTEINE RESIDUES.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M16406 Genomic DNA. Translation: AAA40660.1.
S73971 Genomic DNA. Translation: AAB20705.1.
PIRA29514. A94518.
RefSeqNP_542951.1. NM_080773.1.
XP_006231036.1. XM_006230974.1.
UniGeneRn.119395.

3D structure databases

ProteinModelPortalP08482.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid247270. 1 interaction.
STRING10116.ENSRNOP00000024785.

Chemistry

BindingDBP08482.
ChEMBLCHEMBL1907609.
GuidetoPHARMACOLOGY13.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP08482.

Proteomic databases

PRIDEP08482.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000024785; ENSRNOP00000024785; ENSRNOG00000018385.
GeneID25229.
KEGGrno:25229.
UCSCRGD:2342. rat.

Organism-specific databases

CTD1128.
RGD2342. Chrm1.

Phylogenomic databases

eggNOGNOG250863.
GeneTreeENSGT00690000101881.
HOGENOMHOG000231484.
HOVERGENHBG105720.
InParanoidP08482.
KOK04129.
OMARCCRTPR.
OrthoDBEOG7V49Z7.
PhylomeDBP08482.
TreeFamTF320495.

Gene expression databases

GenevestigatorP08482.

Family and domain databases

Gene3D1.20.1070.10. 2 hits.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002228. Musac_Ach_M1_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERPTHR24249:SF59. PTHR24249:SF59. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00538. MUSCRINICM1R.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio605783.
PROP08482.

Entry information

Entry nameACM1_RAT
AccessionPrimary (citable) accession number: P08482
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: April 16, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries