ID   G3PC_HORVU              Reviewed;         305 AA.
AC   P08477;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1988, sequence version 1.
DT   16-JUN-2009, entry version 67.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase, cytosolic;
DE            EC=1.2.1.12;
DE   Flags: Fragment;
GN   Name=GAPC;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade;
OC   Pooideae; Triticeae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Chojecki J.;
RT   "Identification and characterisation of a cDNA clone for cytosolic
RT   glyceraldehyde-3-phosphate dehydrogenase in barley.";
RL   Carlsberg Res. Commun. 51:203-210(1986).
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 1/5.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- MISCELLANEOUS: Plants contain three forms of GAPDH: a cytosolic
CC       form which participates in glycolysis and two chloroplast forms
CC       which participates in photosynthesis. These three forms are
CC       encoded by distinct genes.
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family.
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DR   EMBL; M36650; AAA32956.1; -; mRNA.
DR   PIR; A24159; A24159.
DR   UniGene; Hv.9630; -.
DR   HSSP; P56649; 1DSS.
DR   Gramene; P08477; -.
DR   BRENDA; 1.2.1.12; 283.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (p...; IEA:EC.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006096; P:glycolysis; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000173; GlycerAld_3-P_DH.
DR   InterPro; IPR006424; Glyceraldehyde-3-P_DH_1.
DR   PANTHER; PTHR10836; GAP_DH; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PIRSF; PIRSF000149; GAP_DH; 1.
DR   PRINTS; PR00078; G3PDHDRGNASE.
DR   TIGRFAMs; TIGR01534; GAPDH-I; 1.
DR   PROSITE; PS00071; GAPDH; PARTIAL.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Glycolysis; NAD; Oxidoreductase.
FT   CHAIN        <1    305       Glyceraldehyde-3-phosphate dehydrogenase,
FT                                cytosolic.
FT                                /FTId=PRO_0000145603.
FT   REGION      121    123       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   REGION      181    182       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   ACT_SITE    122    122       Nucleophile (By similarity).
FT   BINDING       3      3       NAD (By similarity).
FT   BINDING      50     50       NAD; via carbonyl oxygen (By similarity).
FT   BINDING     152    152       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     204    204       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     286    286       NAD (By similarity).
FT   SITE        149    149       Activates thiol group during catalysis
FT                                (By similarity).
FT   NON_TER       1      1
SQ   SEQUENCE   305 AA;  33236 MW;  4F82FA362F222AC5 CRC64;
     VNDPFITTDY MTYMFKYDTV HGQWKHHEVK VKDSKTLLFG EKEVAVFGCR NPEEIPWAAA
     GAEYVVESTG VFTDKDKAAA HIKGGAKKVI ISAPSKDAPM FVCGVNEKEY KSDIDIVSNA
     SCTTNCPAPL AKVINDRFGI VEGLMTTVHA MTATQKTVDG PSSKDWRGGR AASFNIIPSS
     TGAAKAVGKV LPELNGKLTG MAFRVPTVDV SVVDLTVRLA KPATYEQIKA AIKEESEGNL
     KGILGYVDED LVSTDFQGDS RSSIFDAKAG IALNDNFVKL VSWYDNEWGY STRVVDLIRH
     MHSTK
//