P08477 (G3PC_HORVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase, cytosolic
EC=1.2.1.12

Gene names

Name:

GAPC

Organism

Hordeum vulgare (Barley)

Taxonomic identifier

4513 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	305 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Miscellaneous	Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 305	›305	Glyceraldehyde-3-phosphate dehydrogenase, cytosolic		PRO_0000145603
Regions
	Region	121 – 123	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	181 – 182	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	122	1	Nucleophile By similarity
	Binding site	3	1	NAD By similarity
	Binding site	50	1	NAD; via carbonyl oxygen By similarity
	Binding site	152	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	204	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	286	1	NAD By similarity
	Site	149	1	Activates thiol group during catalysis By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P08477 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: 4F82FA362F222AC5	FASTA	305	33,236
	10 20 30 40 50 60 VNDPFITTDY MTYMFKYDTV HGQWKHHEVK VKDSKTLLFG EKEVAVFGCR NPEEIPWAAA 70 80 90 100 110 120 GAEYVVESTG VFTDKDKAAA HIKGGAKKVI ISAPSKDAPM FVCGVNEKEY KSDIDIVSNA 130 140 150 160 170 180 SCTTNCPAPL AKVINDRFGI VEGLMTTVHA MTATQKTVDG PSSKDWRGGR AASFNIIPSS 190 200 210 220 230 240 TGAAKAVGKV LPELNGKLTG MAFRVPTVDV SVVDLTVRLA KPATYEQIKA AIKEESEGNL 250 260 270 280 290 300 KGILGYVDED LVSTDFQGDS RSSIFDAKAG IALNDNFVKL VSWYDNEWGY STRVVDLIRH MHSTK

References

[1]	"Identification and characterisation of a cDNA clone for cytosolic glyceraldehyde-3-phosphate dehydrogenase in barley." Chojecki J. Carlsberg Res. Commun. 51:203-210(1986) Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M36650 mRNA. Translation: AAA32956.1.
PIR	A24159.
UniGene	Hv.9630.
3D structure databases
ProteinModelPortal	P08477.
SMR	P08477. Positions 1-302.
ModBase	Search...
Protein-protein interaction databases
IntAct	P08477. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P08477.
Gene expression databases
Genevestigator	P08477.
Family and domain databases
InterPro	IPR020831. GlycerAld/Erythrose_P_DH. IPR020829. GlycerAld_3-P_DH_cat. IPR020828. GlycerAld_3-P_DH_NAD(P)-bd. IPR006424. Glyceraldehyde-3-P_DH_1. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3PC_HORVU

Accession

Primary (citable) accession number: P08477

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	December 14, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program