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Reviewed, UniProtKB/Swiss-Prot P08477 (G3PC_HORVU)

Last modified June 16, 2009. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Glyceraldehyde-3-phosphate dehydrogenase, cytosolic
    EC=1.2.1.12
Gene names
Name: GAPC
OrganismHordeum vulgare (Barley)
Taxonomic identifier4513 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

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Protein attributes

Sequence length305 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm.

Miscellaneous

Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

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Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological processglycolysis

Inferred from electronic annotation. Source: UniProtKB-KW

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionNAD or NADH binding

Inferred from electronic annotation. Source: InterPro

glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 305›305Glyceraldehyde-3-phosphate dehydrogenase, cytosolic
PRO_0000145603

Regions

Region121 – 1233Glyceraldehyde 3-phosphate binding By similarity
Region181 – 1822Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site1221Nucleophile By similarity
Binding site31NAD By similarity
Binding site501NAD; via carbonyl oxygen By similarity
Binding site1521Glyceraldehyde 3-phosphate By similarity
Binding site2041Glyceraldehyde 3-phosphate By similarity
Binding site2861NAD By similarity
Site1491Activates thiol group during catalysis By similarity

Experimental info

Non-terminal residue11

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Sequences

Sequence LengthMass (Da)Tools
P08477-1 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: 4F82FA362F222AC5

FASTA30533,236
        10         20         30         40         50         60 
VNDPFITTDY MTYMFKYDTV HGQWKHHEVK VKDSKTLLFG EKEVAVFGCR NPEEIPWAAA 

        70         80         90        100        110        120 
GAEYVVESTG VFTDKDKAAA HIKGGAKKVI ISAPSKDAPM FVCGVNEKEY KSDIDIVSNA 

       130        140        150        160        170        180 
SCTTNCPAPL AKVINDRFGI VEGLMTTVHA MTATQKTVDG PSSKDWRGGR AASFNIIPSS 

       190        200        210        220        230        240 
TGAAKAVGKV LPELNGKLTG MAFRVPTVDV SVVDLTVRLA KPATYEQIKA AIKEESEGNL 

       250        260        270        280        290        300 
KGILGYVDED LVSTDFQGDS RSSIFDAKAG IALNDNFVKL VSWYDNEWGY STRVVDLIRH 


MHSTK

« Hide

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References

[1]"Identification and characterisation of a cDNA clone for cytosolic glyceraldehyde-3-phosphate dehydrogenase in barley."
Chojecki J.
Carlsberg Res. Commun. 51:203-210(1986)
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

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Cross-references

Sequence databases

M36650 mRNA. Translation: AAA32956.1.
PIRA24159.
UniGeneHv.9630

3D structure databases

HSSPHSSP built from PDB template 1DSS based on UniProtKB P56649.
ModBaseSearch...

Organism-specific databases

GrameneP08477.

Enzyme and pathway databases

BRENDA1.2.1.12. 283.

Family and domain databases

InterProIPR000173. GlycerAld_3-P_DH.
IPR006424. Glyceraldehyde-3-P_DH_1.
[Graphical view]
PANTHERPTHR10836. GAP_DH. 1 hit.
PfamPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000149. GAP_DH. 1 hit.
PRINTSPR00078. G3PDHDRGNASE.
TIGRFAMsTIGR01534. GAPDH-I. 1 hit.
PROSITEPS00071. GAPDH. Partial match.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameG3PC_HORVU
AccessionPrimary (citable) accession number: P08477
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents