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P08475

- RBS2_CHLRE

UniProt

P08475 - RBS2_CHLRE

Protein

Ribulose bisphosphate carboxylase small chain 2, chloroplastic

Gene

RBCS-2

Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.

    Catalytic activityi

    2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.
    3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.

    GO - Molecular functioni

    1. monooxygenase activity Source: UniProtKB-KW
    2. ribulose-bisphosphate carboxylase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbon fixation Source: UniProtKB-KW
    2. photorespiration Source: UniProtKB-KW
    3. photosynthesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Lyase, Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Carbon dioxide fixation, Photorespiration, Photosynthesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribulose bisphosphate carboxylase small chain 2, chloroplastic (EC:4.1.1.39)
    Short name:
    RuBisCO small subunit 2
    Gene namesi
    Name:RBCS-2
    OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
    Taxonomic identifieri3055 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4545ChloroplastAdd
    BLAST
    Chaini46 – 185140Ribulose bisphosphate carboxylase small chain 2, chloroplasticPRO_0000031473Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei46 – 461N-methylmethionine

    Keywords - PTMi

    Methylation

    Interactioni

    Subunit structurei

    8 large chains + 8 small chains.1 Publication

    Structurei

    Secondary structure

    1
    185
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni59 – 624
    Helixi68 – 8013
    Beta strandi84 – 907
    Helixi92 – 943
    Helixi100 – 1045
    Helixi131 – 14414
    Beta strandi148 – 1569
    Turni157 – 1604
    Beta strandi161 – 1699
    Helixi180 – 1823

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1IR2X-ray1.841/2/3/4/5/6/7/8/I/J/K/L/M/N/O/P46-185[»]
    1UZHX-ray2.20C/F/I/J/M/P/T/W-[»]
    ProteinModelPortaliP08475.
    SMRiP08475. Positions 46-185.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP08475.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RuBisCO small chain family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG4451.
    OMAiRCLAFDN.

    Family and domain databases

    Gene3Di3.30.190.10. 1 hit.
    InterProiIPR024681. RuBisCO_sc.
    IPR000894. RuBisCO_sc_dom.
    [Graphical view]
    PfamiPF00101. RuBisCO_small. 1 hit.
    [Graphical view]
    PRINTSiPR00152. RUBISCOSMALL.
    SMARTiSM00961. RuBisCO_small. 1 hit.
    [Graphical view]
    SUPFAMiSSF55239. SSF55239. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P08475-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAVIAKSSV SAAVARPARS SVRPMAALKP AVKAAPVAAP AQANQMMVWT    50
    PVNNKMFETF SYLPPLSDEQ IAAQVDYIVA NGWIPCLEFA ESDKAYVSNE 100
    SAIRFGSVSC LYYDNRYWTM WKLPMFGCRD PMQVLREIVA CTKAFPDAYV 150
    RLVAFDNQKQ VQIMGFLVQR PKSARDWQPA NKRSV 185
    Length:185
    Mass (Da):20,647
    Last modified:August 1, 1988 - v1
    Checksum:iE19A3627EF484F50
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04472 Genomic DNA. Translation: CAA28160.1.
    PIRiB25785. RKKMS2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04472 Genomic DNA. Translation: CAA28160.1 .
    PIRi B25785. RKKMS2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1IR2 X-ray 1.84 1/2/3/4/5/6/7/8/I/J/K/L/M/N/O/P 46-185 [» ]
    1UZH X-ray 2.20 C/F/I/J/M/P/T/W - [» ]
    ProteinModelPortali P08475.
    SMRi P08475. Positions 46-185.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG4451.
    OMAi RCLAFDN.

    Miscellaneous databases

    EvolutionaryTracei P08475.

    Family and domain databases

    Gene3Di 3.30.190.10. 1 hit.
    InterProi IPR024681. RuBisCO_sc.
    IPR000894. RuBisCO_sc_dom.
    [Graphical view ]
    Pfami PF00101. RuBisCO_small. 1 hit.
    [Graphical view ]
    PRINTSi PR00152. RUBISCOSMALL.
    SMARTi SM00961. RuBisCO_small. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55239. SSF55239. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii."
      Goldschmidt-Clermont M., Rahire M.
      J. Mol. Biol. 191:421-432(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Partial purification and properties of enzymes involved in the processing of a chloroplast import protein from Chlamydomonas reinhardii."
      Su Q., Boschetti A.
      Eur. J. Biochem. 217:1039-1047(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-60.
    3. "Crystal structure of activated ribulose-1,5-bisphosphate carboxylase/oxygenase from green alga Chlamydomonas reinhardtii complexed with 2-carboxyarabinitol-1,5-bisphosphate."
      Mizohata E., Matsumura H., Okano Y., Kumei M., Takuma H., Onodera J., Kato K., Shibata N., Inoue T., Yokota A., Kai Y.
      J. Mol. Biol. 316:679-691(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 46-185 IN COMPLEX WITH THE TRANSITION-STATE ANALOG 2-CABP.
      Strain: 137c / CC-125.

    Entry informationi

    Entry nameiRBS2_CHLRE
    AccessioniPrimary (citable) accession number: P08475
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3