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P08475

- RBS2_CHLRE

UniProt

P08475 - RBS2_CHLRE

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Protein

Ribulose bisphosphate carboxylase small chain 2, chloroplastic

Gene

RBCS-2

Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.

GO - Molecular functioni

  1. monooxygenase activity Source: UniProtKB-KW
  2. ribulose-bisphosphate carboxylase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbon fixation Source: UniProtKB-KW
  2. photorespiration Source: UniProtKB-KW
  3. photosynthesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Monooxygenase, Oxidoreductase

Keywords - Biological processi

Carbon dioxide fixation, Photorespiration, Photosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylase small chain 2, chloroplastic (EC:4.1.1.39)
Short name:
RuBisCO small subunit 2
Gene namesi
Name:RBCS-2
OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifieri3055 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4545ChloroplastAdd
BLAST
Chaini46 – 185140Ribulose bisphosphate carboxylase small chain 2, chloroplasticPRO_0000031473Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei46 – 461N-methylmethionine1 Publication

Keywords - PTMi

Methylation

Interactioni

Subunit structurei

8 large chains + 8 small chains.1 Publication

Structurei

Secondary structure

1
185
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni59 – 624Combined sources
Helixi68 – 8013Combined sources
Beta strandi84 – 907Combined sources
Helixi92 – 943Combined sources
Helixi100 – 1045Combined sources
Helixi131 – 14414Combined sources
Beta strandi148 – 1569Combined sources
Turni157 – 1604Combined sources
Beta strandi161 – 1699Combined sources
Helixi180 – 1823Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IR2X-ray1.841/2/3/4/5/6/7/8/I/J/K/L/M/N/O/P46-185[»]
1UZHX-ray2.20C/F/I/J/M/P/T/W-[»]
ProteinModelPortaliP08475.
SMRiP08475. Positions 46-185.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08475.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuBisCO small chain family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG4451.
OMAiRCLAFDN.

Family and domain databases

Gene3Di3.30.190.10. 1 hit.
InterProiIPR024681. RuBisCO_sc.
IPR000894. RuBisCO_sc_dom.
[Graphical view]
PfamiPF00101. RuBisCO_small. 1 hit.
[Graphical view]
PRINTSiPR00152. RUBISCOSMALL.
SMARTiSM00961. RuBisCO_small. 1 hit.
[Graphical view]
SUPFAMiSSF55239. SSF55239. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08475-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAVIAKSSV SAAVARPARS SVRPMAALKP AVKAAPVAAP AQANQMMVWT
60 70 80 90 100
PVNNKMFETF SYLPPLSDEQ IAAQVDYIVA NGWIPCLEFA ESDKAYVSNE
110 120 130 140 150
SAIRFGSVSC LYYDNRYWTM WKLPMFGCRD PMQVLREIVA CTKAFPDAYV
160 170 180
RLVAFDNQKQ VQIMGFLVQR PKSARDWQPA NKRSV
Length:185
Mass (Da):20,647
Last modified:August 1, 1988 - v1
Checksum:iE19A3627EF484F50
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04472 Genomic DNA. Translation: CAA28160.1.
PIRiB25785. RKKMS2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04472 Genomic DNA. Translation: CAA28160.1 .
PIRi B25785. RKKMS2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1IR2 X-ray 1.84 1/2/3/4/5/6/7/8/I/J/K/L/M/N/O/P 46-185 [» ]
1UZH X-ray 2.20 C/F/I/J/M/P/T/W - [» ]
ProteinModelPortali P08475.
SMRi P08475. Positions 46-185.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG4451.
OMAi RCLAFDN.

Miscellaneous databases

EvolutionaryTracei P08475.

Family and domain databases

Gene3Di 3.30.190.10. 1 hit.
InterProi IPR024681. RuBisCO_sc.
IPR000894. RuBisCO_sc_dom.
[Graphical view ]
Pfami PF00101. RuBisCO_small. 1 hit.
[Graphical view ]
PRINTSi PR00152. RUBISCOSMALL.
SMARTi SM00961. RuBisCO_small. 1 hit.
[Graphical view ]
SUPFAMi SSF55239. SSF55239. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii."
    Goldschmidt-Clermont M., Rahire M.
    J. Mol. Biol. 191:421-432(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Partial purification and properties of enzymes involved in the processing of a chloroplast import protein from Chlamydomonas reinhardii."
    Su Q., Boschetti A.
    Eur. J. Biochem. 217:1039-1047(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-60.
  3. "Crystal structure of activated ribulose-1,5-bisphosphate carboxylase/oxygenase from green alga Chlamydomonas reinhardtii complexed with 2-carboxyarabinitol-1,5-bisphosphate."
    Mizohata E., Matsumura H., Okano Y., Kumei M., Takuma H., Onodera J., Kato K., Shibata N., Inoue T., Yokota A., Kai Y.
    J. Mol. Biol. 316:679-691(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 46-185 IN COMPLEX WITH THE TRANSITION-STATE ANALOG 2-CABP, METHYLATION AT MET-46.
    Strain: 137c / CC-125.

Entry informationi

Entry nameiRBS2_CHLRE
AccessioniPrimary (citable) accession number: P08475
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 26, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3