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P08475 (RBS2_CHLRE) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribulose bisphosphate carboxylase small chain 2, chloroplastic

Short name=RuBisCO small subunit 2
EC=4.1.1.39
Gene names
Name:RBCS-2
OrganismChlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifier3055 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

Protein attributes

Sequence length185 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.

Catalytic activity

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.

3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.

Subunit structure

8 large chains + 8 small chains.

Subcellular location

Plastidchloroplast.

Sequence similarities

Belongs to the RuBisCO small chain family.

Ontologies

Keywords
   Biological processCarbon dioxide fixation
Photorespiration
Photosynthesis
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   Molecular functionLyase
Monooxygenase
Oxidoreductase
   PTMMethylation
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological_processcarbon fixation

Inferred from electronic annotation. Source: UniProtKB-KW

photorespiration

Inferred from electronic annotation. Source: UniProtKB-KW

photosynthesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmonooxygenase activity

Inferred from electronic annotation. Source: UniProtKB-KW

ribulose-bisphosphate carboxylase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4545Chloroplast
Chain46 – 185140Ribulose bisphosphate carboxylase small chain 2, chloroplastic
PRO_0000031473

Amino acid modifications

Modified residue461N-methylmethionine

Secondary structure

................... 185
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P08475 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: E19A3627EF484F50

FASTA18520,647
        10         20         30         40         50         60 
MAAVIAKSSV SAAVARPARS SVRPMAALKP AVKAAPVAAP AQANQMMVWT PVNNKMFETF 

        70         80         90        100        110        120 
SYLPPLSDEQ IAAQVDYIVA NGWIPCLEFA ESDKAYVSNE SAIRFGSVSC LYYDNRYWTM 

       130        140        150        160        170        180 
WKLPMFGCRD PMQVLREIVA CTKAFPDAYV RLVAFDNQKQ VQIMGFLVQR PKSARDWQPA 


NKRSV 

« Hide

References

[1]"Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii."
Goldschmidt-Clermont M., Rahire M.
J. Mol. Biol. 191:421-432(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Partial purification and properties of enzymes involved in the processing of a chloroplast import protein from Chlamydomonas reinhardii."
Su Q., Boschetti A.
Eur. J. Biochem. 217:1039-1047(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-60.
[3]"Crystal structure of activated ribulose-1,5-bisphosphate carboxylase/oxygenase from green alga Chlamydomonas reinhardtii complexed with 2-carboxyarabinitol-1,5-bisphosphate."
Mizohata E., Matsumura H., Okano Y., Kumei M., Takuma H., Onodera J., Kato K., Shibata N., Inoue T., Yokota A., Kai Y.
J. Mol. Biol. 316:679-691(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 46-185 IN COMPLEX WITH THE TRANSITION-STATE ANALOG 2-CABP.
Strain: 137c / CC-125.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X04472 Genomic DNA. Translation: CAA28160.1.
PIRRKKMS2. B25785.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1IR2X-ray1.841/2/3/4/5/6/7/8/I/J/K/L/M/N/O/P46-185[»]
1UZHX-ray2.20C/F/I/J/M/P/T/W-[»]
ProteinModelPortalP08475.
SMRP08475. Positions 46-185.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG4451.
OMAKSARDWQ.

Family and domain databases

Gene3D3.30.190.10. 1 hit.
InterProIPR024681. RuBisCO_sc.
IPR000894. RuBisCO_sc_dom.
[Graphical view]
PfamPF00101. RuBisCO_small. 1 hit.
[Graphical view]
PRINTSPR00152. RUBISCOSMALL.
SMARTSM00961. RuBisCO_small. 1 hit.
[Graphical view]
SUPFAMSSF55239. SSF55239. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP08475.

Entry information

Entry nameRBS2_CHLRE
AccessionPrimary (citable) accession number: P08475
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: October 16, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references