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Protein

Mitochondrial nuclease

Gene

NUC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This enzyme has both RNase and DNase activity.

Miscellaneous

Present with 3870 molecules/cell in log phase SD medium.1 Publication
The active site contains 1 hydrated divalent metal cation that has only 1 direct interaction with the protein; all other interactions are via water molecules.By similarity

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei138Proton acceptorPROSITE-ProRule annotation1
Metal bindingi170Magnesium or manganese; catalyticBy similarity1

GO - Molecular functioni

  • endodeoxyribonuclease activity Source: SGD
  • exodeoxyribonuclease activity Source: SGD
  • metal ion binding Source: UniProtKB-KW
  • nucleic acid binding Source: InterPro
  • ribonuclease activity Source: SGD

GO - Biological processi

  • apoptotic DNA fragmentation Source: SGD
  • apoptotic process Source: SGD
  • DNA catabolic process Source: SGD
  • DNA recombination Source: SGD
  • RNA catabolic process Source: SGD

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31636-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial nuclease (EC:3.1.30.-)
Gene namesi
Name:NUC1
Ordered Locus Names:YJL208C
ORF Names:HRE329, J0310
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL208C
SGDiS000003744 NUC1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001786701 – 329Mitochondrial nucleaseAdd BLAST329

Proteomic databases

MaxQBiP08466
PaxDbiP08466
PRIDEiP08466
TopDownProteomicsiP08466

PTM databases

SwissPalmiP08466

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi33550, 128 interactors
DIPiDIP-5041N
IntActiP08466, 6 interactors
STRINGi4932.YJL208C

Structurei

3D structure databases

ProteinModelPortaliP08466
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00530000063772
HOGENOMiHOG000214172
InParanoidiP08466
KOiK01173
OMAiVPHWVFE
OrthoDBiEOG092C46VQ

Family and domain databases

InterProiView protein in InterPro
IPR018524 DNA/RNA_endonuclease_AS
IPR001604 DNA/RNA_non-sp_Endonuclease
IPR020821 Extracellular_endonuc_su_A
PfamiView protein in Pfam
PF01223 Endonuclease_NS, 1 hit
SMARTiView protein in SMART
SM00892 Endonuclease_NS, 1 hit
SM00477 NUC, 1 hit
PROSITEiView protein in PROSITE
PS01070 NUCLEASE_NON_SPEC, 1 hit

Sequencei

Sequence statusi: Complete.

P08466-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCSRILLSGL VGLGAGTGLT YLLLNKHSPT QIIETPYPPT QKPNSNIQSH
60 70 80 90 100
SFNVDPSGFF KYGFPGPIHD LQNREEFISC YNRQTQNPYW VLEHITPESL
110 120 130 140 150
AARNADRKNS FFKEDEVIPE KFRGKLRDYF RSGYDRGHQA PAADAKFSQQ
160 170 180 190 200
AMDDTFYLSN MCPQVGEGFN RDYWAHLEYF CRGLTKKYKS VRIVTGPLYL
210 220 230 240 250
PKKDPIDNKF RVNYEVIGNP PSIAVPTHFF KLIVAEAPTA NPAREDIAVA
260 270 280 290 300
AFVLPNEPIS NETKLTDFEV PIDALERSTG LELLQKVPPS KKKALCKEVN
310 320
CQIVVRDFSN AAIKQSKDVK LLPPPKKRN
Length:329
Mass (Da):37,210
Last modified:August 1, 1988 - v1
Checksum:iCAEC9678CB00943C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06670 Genomic DNA Translation: CAA29870.1
X77688 Genomic DNA Translation: CAA54748.1
Z34098 Genomic DNA Translation: CAA84003.1
Z49483 Genomic DNA Translation: CAA89505.1
M28067 Genomic DNA Translation: AAA34457.1
BK006943 Genomic DNA Translation: DAA08602.1
PIRiS05888 NCBYN1
RefSeqiNP_012327.1, NM_001181641.1

Genome annotation databases

EnsemblFungiiYJL208C; YJL208C; YJL208C
GeneIDi853222
KEGGisce:YJL208C

Similar proteinsi

Entry informationi

Entry nameiNUC1_YEAST
AccessioniPrimary (citable) accession number: P08466
Secondary accession number(s): D6VVY6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: May 23, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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