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P08466

- NUC1_YEAST

UniProt

P08466 - NUC1_YEAST

Protein

Mitochondrial nuclease

Gene

NUC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    This enzyme has both RNase and DNase activity.

    Cofactori

    Manganese or magnesium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei138 – 1381Proton acceptorPROSITE-ProRule annotation
    Metal bindingi170 – 1701Magnesium or manganese; catalyticBy similarity

    GO - Molecular functioni

    1. endodeoxyribonuclease activity Source: SGD
    2. exodeoxyribonuclease activity Source: SGD
    3. metal ion binding Source: UniProtKB-KW
    4. nucleic acid binding Source: InterPro
    5. ribonuclease activity Source: SGD

    GO - Biological processi

    1. apoptotic DNA fragmentation Source: SGD
    2. apoptotic process Source: SGD
    3. DNA catabolic process Source: SGD
    4. DNA catabolic process, exonucleolytic Source: GOC
    5. DNA recombination Source: SGD
    6. RNA catabolic process Source: SGD
    7. RNA phosphodiester bond hydrolysis Source: GOC

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31636-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial nuclease (EC:3.1.30.-)
    Gene namesi
    Name:NUC1
    Ordered Locus Names:YJL208C
    ORF Names:HRE329, J0310
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL208c.
    SGDiS000003744. NUC1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 329329Mitochondrial nucleasePRO_0000178670Add
    BLAST

    Proteomic databases

    MaxQBiP08466.
    PaxDbiP08466.
    PeptideAtlasiP08466.

    Expressioni

    Gene expression databases

    GenevestigatoriP08466.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    BioGridi33550. 45 interactions.
    DIPiDIP-5041N.
    IntActiP08466. 5 interactions.
    MINTiMINT-474953.
    STRINGi4932.YJL208C.

    Structurei

    3D structure databases

    ProteinModelPortaliP08466.
    SMRiP08466. Positions 61-287.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1864.
    GeneTreeiENSGT00530000063772.
    HOGENOMiHOG000214172.
    KOiK01173.
    OMAiPNARIPN.
    OrthoDBiEOG7034SQ.

    Family and domain databases

    Gene3Di3.40.570.10. 1 hit.
    InterProiIPR018524. DNA/RNA_endonuclease_AS.
    IPR001604. DNA/RNA_non-sp_Endonuclease.
    IPR020821. Extracellular_endonuc_su_A.
    [Graphical view]
    PfamiPF01223. Endonuclease_NS. 1 hit.
    [Graphical view]
    SMARTiSM00892. Endonuclease_NS. 1 hit.
    SM00477. NUC. 1 hit.
    [Graphical view]
    PROSITEiPS01070. NUCLEASE_NON_SPEC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P08466-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCSRILLSGL VGLGAGTGLT YLLLNKHSPT QIIETPYPPT QKPNSNIQSH    50
    SFNVDPSGFF KYGFPGPIHD LQNREEFISC YNRQTQNPYW VLEHITPESL 100
    AARNADRKNS FFKEDEVIPE KFRGKLRDYF RSGYDRGHQA PAADAKFSQQ 150
    AMDDTFYLSN MCPQVGEGFN RDYWAHLEYF CRGLTKKYKS VRIVTGPLYL 200
    PKKDPIDNKF RVNYEVIGNP PSIAVPTHFF KLIVAEAPTA NPAREDIAVA 250
    AFVLPNEPIS NETKLTDFEV PIDALERSTG LELLQKVPPS KKKALCKEVN 300
    CQIVVRDFSN AAIKQSKDVK LLPPPKKRN 329
    Length:329
    Mass (Da):37,210
    Last modified:August 1, 1988 - v1
    Checksum:iCAEC9678CB00943C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06670 Genomic DNA. Translation: CAA29870.1.
    X77688 Genomic DNA. Translation: CAA54748.1.
    Z34098 Genomic DNA. Translation: CAA84003.1.
    Z49483 Genomic DNA. Translation: CAA89505.1.
    M28067 Genomic DNA. Translation: AAA34457.1.
    BK006943 Genomic DNA. Translation: DAA08602.1.
    PIRiS05888. NCBYN1.
    RefSeqiNP_012327.1. NM_001181641.1.

    Genome annotation databases

    EnsemblFungiiYJL208C; YJL208C; YJL208C.
    GeneIDi853222.
    KEGGisce:YJL208C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06670 Genomic DNA. Translation: CAA29870.1 .
    X77688 Genomic DNA. Translation: CAA54748.1 .
    Z34098 Genomic DNA. Translation: CAA84003.1 .
    Z49483 Genomic DNA. Translation: CAA89505.1 .
    M28067 Genomic DNA. Translation: AAA34457.1 .
    BK006943 Genomic DNA. Translation: DAA08602.1 .
    PIRi S05888. NCBYN1.
    RefSeqi NP_012327.1. NM_001181641.1.

    3D structure databases

    ProteinModelPortali P08466.
    SMRi P08466. Positions 61-287.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33550. 45 interactions.
    DIPi DIP-5041N.
    IntActi P08466. 5 interactions.
    MINTi MINT-474953.
    STRINGi 4932.YJL208C.

    Proteomic databases

    MaxQBi P08466.
    PaxDbi P08466.
    PeptideAtlasi P08466.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL208C ; YJL208C ; YJL208C .
    GeneIDi 853222.
    KEGGi sce:YJL208C.

    Organism-specific databases

    CYGDi YJL208c.
    SGDi S000003744. NUC1.

    Phylogenomic databases

    eggNOGi COG1864.
    GeneTreei ENSGT00530000063772.
    HOGENOMi HOG000214172.
    KOi K01173.
    OMAi PNARIPN.
    OrthoDBi EOG7034SQ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31636-MONOMER.

    Miscellaneous databases

    NextBioi 973422.
    PROi P08466.

    Gene expression databases

    Genevestigatori P08466.

    Family and domain databases

    Gene3Di 3.40.570.10. 1 hit.
    InterProi IPR018524. DNA/RNA_endonuclease_AS.
    IPR001604. DNA/RNA_non-sp_Endonuclease.
    IPR020821. Extracellular_endonuc_su_A.
    [Graphical view ]
    Pfami PF01223. Endonuclease_NS. 1 hit.
    [Graphical view ]
    SMARTi SM00892. Endonuclease_NS. 1 hit.
    SM00477. NUC. 1 hit.
    [Graphical view ]
    PROSITEi PS01070. NUCLEASE_NON_SPEC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and expression of NUC1, the gene encoding the mitochondrial nuclease in Saccharomyces cerevisiae."
      Vincent R.D., Hofmann T.J., Zassenhaus H.P.
      Nucleic Acids Res. 16:3297-3312(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 2935-106.
    2. "The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes."
      Purnelle B., Coster F., Goffeau A.
      Yeast 10:1235-1249(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Sequence analysis of a 40.2 kb DNA fragment located near the left telomere of yeast chromosome X."
      Vandenbol M., Durand P., Bolle P.-A., Dion C., Portetelle D., Hilger F.
      Yeast 10:1657-1662(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Overproduction of yeast viruslike particles by strains deficient in a mitochondrial nuclease."
      Liu Y., Dieckmann C.L.
      Mol. Cell. Biol. 9:3323-3331(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 204-329.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNUC1_YEAST
    AccessioniPrimary (citable) accession number: P08466
    Secondary accession number(s): D6VVY6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3870 molecules/cell in log phase SD medium.1 Publication
    The active site contains 1 hydrated divalent metal cation that has only 1 direct interaction with the protein; all other interactions are via water molecules.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3