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P08466

- NUC1_YEAST

UniProt

P08466 - NUC1_YEAST

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Protein

Mitochondrial nuclease

Gene

NUC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

This enzyme has both RNase and DNase activity.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei138 – 1381Proton acceptorPROSITE-ProRule annotation
Metal bindingi170 – 1701Magnesium or manganese; catalyticBy similarity

GO - Molecular functioni

  1. endodeoxyribonuclease activity Source: SGD
  2. exodeoxyribonuclease activity Source: SGD
  3. metal ion binding Source: UniProtKB-KW
  4. nucleic acid binding Source: InterPro
  5. ribonuclease activity Source: SGD

GO - Biological processi

  1. apoptotic DNA fragmentation Source: SGD
  2. apoptotic process Source: SGD
  3. DNA catabolic process Source: SGD
  4. DNA catabolic process, exonucleolytic Source: GOC
  5. DNA recombination Source: SGD
  6. RNA catabolic process Source: SGD
  7. RNA phosphodiester bond hydrolysis Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31636-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial nuclease (EC:3.1.30.-)
Gene namesi
Name:NUC1
Ordered Locus Names:YJL208C
ORF Names:HRE329, J0310
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL208c.
SGDiS000003744. NUC1.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial inner membrane Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329Mitochondrial nucleasePRO_0000178670Add
BLAST

Proteomic databases

MaxQBiP08466.
PaxDbiP08466.
PeptideAtlasiP08466.

Expressioni

Gene expression databases

GenevestigatoriP08466.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi33550. 45 interactions.
DIPiDIP-5041N.
IntActiP08466. 5 interactions.
MINTiMINT-474953.
STRINGi4932.YJL208C.

Structurei

3D structure databases

ProteinModelPortaliP08466.
SMRiP08466. Positions 61-287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1864.
GeneTreeiENSGT00530000063772.
HOGENOMiHOG000214172.
InParanoidiP08466.
KOiK01173.
OMAiPNARIPN.
OrthoDBiEOG7034SQ.

Family and domain databases

Gene3Di3.40.570.10. 1 hit.
InterProiIPR018524. DNA/RNA_endonuclease_AS.
IPR001604. DNA/RNA_non-sp_Endonuclease.
IPR020821. Extracellular_endonuc_su_A.
[Graphical view]
PfamiPF01223. Endonuclease_NS. 1 hit.
[Graphical view]
SMARTiSM00892. Endonuclease_NS. 1 hit.
SM00477. NUC. 1 hit.
[Graphical view]
PROSITEiPS01070. NUCLEASE_NON_SPEC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08466-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCSRILLSGL VGLGAGTGLT YLLLNKHSPT QIIETPYPPT QKPNSNIQSH
60 70 80 90 100
SFNVDPSGFF KYGFPGPIHD LQNREEFISC YNRQTQNPYW VLEHITPESL
110 120 130 140 150
AARNADRKNS FFKEDEVIPE KFRGKLRDYF RSGYDRGHQA PAADAKFSQQ
160 170 180 190 200
AMDDTFYLSN MCPQVGEGFN RDYWAHLEYF CRGLTKKYKS VRIVTGPLYL
210 220 230 240 250
PKKDPIDNKF RVNYEVIGNP PSIAVPTHFF KLIVAEAPTA NPAREDIAVA
260 270 280 290 300
AFVLPNEPIS NETKLTDFEV PIDALERSTG LELLQKVPPS KKKALCKEVN
310 320
CQIVVRDFSN AAIKQSKDVK LLPPPKKRN
Length:329
Mass (Da):37,210
Last modified:August 1, 1988 - v1
Checksum:iCAEC9678CB00943C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06670 Genomic DNA. Translation: CAA29870.1.
X77688 Genomic DNA. Translation: CAA54748.1.
Z34098 Genomic DNA. Translation: CAA84003.1.
Z49483 Genomic DNA. Translation: CAA89505.1.
M28067 Genomic DNA. Translation: AAA34457.1.
BK006943 Genomic DNA. Translation: DAA08602.1.
PIRiS05888. NCBYN1.
RefSeqiNP_012327.1. NM_001181641.1.

Genome annotation databases

EnsemblFungiiYJL208C; YJL208C; YJL208C.
GeneIDi853222.
KEGGisce:YJL208C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06670 Genomic DNA. Translation: CAA29870.1 .
X77688 Genomic DNA. Translation: CAA54748.1 .
Z34098 Genomic DNA. Translation: CAA84003.1 .
Z49483 Genomic DNA. Translation: CAA89505.1 .
M28067 Genomic DNA. Translation: AAA34457.1 .
BK006943 Genomic DNA. Translation: DAA08602.1 .
PIRi S05888. NCBYN1.
RefSeqi NP_012327.1. NM_001181641.1.

3D structure databases

ProteinModelPortali P08466.
SMRi P08466. Positions 61-287.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33550. 45 interactions.
DIPi DIP-5041N.
IntActi P08466. 5 interactions.
MINTi MINT-474953.
STRINGi 4932.YJL208C.

Proteomic databases

MaxQBi P08466.
PaxDbi P08466.
PeptideAtlasi P08466.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL208C ; YJL208C ; YJL208C .
GeneIDi 853222.
KEGGi sce:YJL208C.

Organism-specific databases

CYGDi YJL208c.
SGDi S000003744. NUC1.

Phylogenomic databases

eggNOGi COG1864.
GeneTreei ENSGT00530000063772.
HOGENOMi HOG000214172.
InParanoidi P08466.
KOi K01173.
OMAi PNARIPN.
OrthoDBi EOG7034SQ.

Enzyme and pathway databases

BioCyci YEAST:G3O-31636-MONOMER.

Miscellaneous databases

NextBioi 973422.
PROi P08466.

Gene expression databases

Genevestigatori P08466.

Family and domain databases

Gene3Di 3.40.570.10. 1 hit.
InterProi IPR018524. DNA/RNA_endonuclease_AS.
IPR001604. DNA/RNA_non-sp_Endonuclease.
IPR020821. Extracellular_endonuc_su_A.
[Graphical view ]
Pfami PF01223. Endonuclease_NS. 1 hit.
[Graphical view ]
SMARTi SM00892. Endonuclease_NS. 1 hit.
SM00477. NUC. 1 hit.
[Graphical view ]
PROSITEi PS01070. NUCLEASE_NON_SPEC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and expression of NUC1, the gene encoding the mitochondrial nuclease in Saccharomyces cerevisiae."
    Vincent R.D., Hofmann T.J., Zassenhaus H.P.
    Nucleic Acids Res. 16:3297-3312(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 2935-106.
  2. "The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes."
    Purnelle B., Coster F., Goffeau A.
    Yeast 10:1235-1249(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Sequence analysis of a 40.2 kb DNA fragment located near the left telomere of yeast chromosome X."
    Vandenbol M., Durand P., Bolle P.-A., Dion C., Portetelle D., Hilger F.
    Yeast 10:1657-1662(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Overproduction of yeast viruslike particles by strains deficient in a mitochondrial nuclease."
    Liu Y., Dieckmann C.L.
    Mol. Cell. Biol. 9:3323-3331(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 204-329.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNUC1_YEAST
AccessioniPrimary (citable) accession number: P08466
Secondary accession number(s): D6VVY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 26, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3870 molecules/cell in log phase SD medium.1 Publication
The active site contains 1 hydrated divalent metal cation that has only 1 direct interaction with the protein; all other interactions are via water molecules.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3