Reviewed, UniProtKB/Swiss-Prot P08466 (NUC1_YEAST)

Names and origin

Protein names

Recommended name:
    Mitochondrial nuclease
    EC=3.1.30.-

Gene names

Name:	NUC1
Ordered Locus Names:	YJL208C
ORF Names:	J0310, HRE329

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This enzyme has both RNase and DNase activity.
Cofactor	Manganese or magnesium.
Subunit structure	Homodimer.
Subcellular location	Mitochondrion inner membrane.
Miscellaneous	Present with 3870 molecules/cell in log phase SD medium. Ref.6 The active site contains 1 hydrated divalent metal cation that has only 1 direct interaction with the protein; all other interactions are via water molecules By similarity.
Sequence similarities	Belongs to the DNA/RNA non-specific endonuclease family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Ligand	Magnesium Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA catabolic process Inferred from direct assay. Source: SGD DNA recombination Inferred from mutant phenotype. Source: SGD RNA catabolic process Inferred from direct assay. Source: SGD apoptosis Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrial inner membrane Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	endodeoxyribonuclease activity Inferred from direct assay. Source: SGD exodeoxyribonuclease activity Inferred from direct assay. Source: SGD magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro ribonuclease activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	Mitochondrial nuclease		PRO_0000178670
Sites
	Active site	138	1	Proton acceptor By similarity
	Metal binding	170	1	Manganese or magnesium; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08466-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: CAEC9678CB00943C	FASTA	329	37,210
	10 20 30 40 50 60 MCSRILLSGL VGLGAGTGLT YLLLNKHSPT QIIETPYPPT QKPNSNIQSH SFNVDPSGFF 70 80 90 100 110 120 KYGFPGPIHD LQNREEFISC YNRQTQNPYW VLEHITPESL AARNADRKNS FFKEDEVIPE 130 140 150 160 170 180 KFRGKLRDYF RSGYDRGHQA PAADAKFSQQ AMDDTFYLSN MCPQVGEGFN RDYWAHLEYF 190 200 210 220 230 240 CRGLTKKYKS VRIVTGPLYL PKKDPIDNKF RVNYEVIGNP PSIAVPTHFF KLIVAEAPTA 250 260 270 280 290 300 NPAREDIAVA AFVLPNEPIS NETKLTDFEV PIDALERSTG LELLQKVPPS KKKALCKEVN 310 320 CQIVVRDFSN AAIKQSKDVK LLPPPKKRN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and expression of NUC1, the gene encoding the mitochondrial nuclease in Saccharomyces cerevisiae." Vincent R.D., Hofmann T.J., Zassenhaus H.P. Nucleic Acids Res. 16:3297-3312(1988) [PubMed: 2836792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 2935-106.
[2]	"The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes." Purnelle B., Coster F., Goffeau A. Yeast 10:1235-1249(1994) [PubMed: 7754713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Sequence analysis of a 40.2 kb DNA fragment located near the left telomere of yeast chromosome X." Vandenbol M., Durand P., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:1657-1662(1994) [PubMed: 7725802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Overproduction of yeast viruslike particles by strains deficient in a mitochondrial nuclease." Liu Y., Dieckmann C.L. Mol. Cell. Biol. 9:3323-3331(1989) [PubMed: 2552292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 204-329.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X06670 Genomic DNA. Translation: CAA29870.1. X77688 Genomic DNA. Translation: CAA54748.1. Z34098 Genomic DNA. Translation: CAA84003.1. Z49483 Genomic DNA. Translation: CAA89505.1. M28067 Genomic DNA. Translation: AAA34457.1.
PIR	NCBYN1. S05888.
RefSeq	NP_012327.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5041N.
IntAct	P08466. 8 interactions.
STRING	P08466.
Proteomic databases
PeptideAtlas	P08466.
Genome annotation databases
Ensembl	YJL208C; YJL208C; YJL208C; Saccharomyces cerevisiae. [Genome view]
GeneID	853222.
KEGG	sce:YJL208C.
NMPDR	fig|4932.3.peg.3290.
Organism-specific databases
CYGD	YJL208c.
SGD	S000003744. NUC1.
Phylogenomic databases
HOGENOM	P08466.
OMA	NIAVPTH
OrthoDB	EOG95HTDT
Gene expression databases
ArrayExpress	P08466.
Genevestigator	P08466.
GermOnline	YJL208C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018524. DNA/RNA_endonuclease_AS. IPR001604. DNA/RNA_non-sp_Endonuclease. IPR020821. Extracellular_endonuc_su_A. [Graphical view]
Gene3D	G3DSA:3.40.570.10. Endonuclease. 1 hit.
Pfam	PF01223. Endonuclease_NS. 1 hit. [Graphical view]
SMART	SM00892. Endonuclease_NS. 1 hit. SM00477. NUC. 1 hit. [Graphical view]
PROSITE	PS01070. NUCLEASE_NON_SPEC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973422.

Entry information

Entry name

NUC1_YEAST

Accession

Primary (citable) accession number: P08466

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	November 24, 2009
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)