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P08466 (NUC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial nuclease

EC=3.1.30.-
Gene names
Name:NUC1
Ordered Locus Names:YJL208C
ORF Names:HRE329, J0310
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This enzyme has both RNase and DNase activity.

Cofactor

Manganese or magnesium.

Subunit structure

Homodimer.

Subcellular location

Mitochondrion inner membrane.

Miscellaneous

Present with 3870 molecules/cell in log phase SD medium.

The active site contains 1 hydrated divalent metal cation that has only 1 direct interaction with the protein; all other interactions are via water molecules By similarity.

Sequence similarities

Belongs to the DNA/RNA non-specific endonuclease family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329Mitochondrial nuclease
PRO_0000178670

Sites

Active site1381Proton acceptor By similarity
Metal binding1701Magnesium or manganese; catalytic By similarity

Sequences

Sequence LengthMass (Da)Tools
P08466 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: CAEC9678CB00943C

FASTA32937,210
        10         20         30         40         50         60 
MCSRILLSGL VGLGAGTGLT YLLLNKHSPT QIIETPYPPT QKPNSNIQSH SFNVDPSGFF 

        70         80         90        100        110        120 
KYGFPGPIHD LQNREEFISC YNRQTQNPYW VLEHITPESL AARNADRKNS FFKEDEVIPE 

       130        140        150        160        170        180 
KFRGKLRDYF RSGYDRGHQA PAADAKFSQQ AMDDTFYLSN MCPQVGEGFN RDYWAHLEYF 

       190        200        210        220        230        240 
CRGLTKKYKS VRIVTGPLYL PKKDPIDNKF RVNYEVIGNP PSIAVPTHFF KLIVAEAPTA 

       250        260        270        280        290        300 
NPAREDIAVA AFVLPNEPIS NETKLTDFEV PIDALERSTG LELLQKVPPS KKKALCKEVN 

       310        320 
CQIVVRDFSN AAIKQSKDVK LLPPPKKRN 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and expression of NUC1, the gene encoding the mitochondrial nuclease in Saccharomyces cerevisiae."
Vincent R.D., Hofmann T.J., Zassenhaus H.P.
Nucleic Acids Res. 16:3297-3312(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 2935-106.
[2]"The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes."
Purnelle B., Coster F., Goffeau A.
Yeast 10:1235-1249(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Sequence analysis of a 40.2 kb DNA fragment located near the left telomere of yeast chromosome X."
Vandenbol M., Durand P., Bolle P.-A., Dion C., Portetelle D., Hilger F.
Yeast 10:1657-1662(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Overproduction of yeast viruslike particles by strains deficient in a mitochondrial nuclease."
Liu Y., Dieckmann C.L.
Mol. Cell. Biol. 9:3323-3331(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 204-329.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X06670 Genomic DNA. Translation: CAA29870.1.
X77688 Genomic DNA. Translation: CAA54748.1.
Z34098 Genomic DNA. Translation: CAA84003.1.
Z49483 Genomic DNA. Translation: CAA89505.1.
M28067 Genomic DNA. Translation: AAA34457.1.
BK006943 Genomic DNA. Translation: DAA08602.1.
PIRNCBYN1. S05888.
RefSeqNP_012327.1. NM_001181641.1.

3D structure databases

ProteinModelPortalP08466.
SMRP08466. Positions 61-287.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33550. 45 interactions.
DIPDIP-5041N.
IntActP08466. 5 interactions.
MINTMINT-474953.
STRING4932.YJL208C.

Proteomic databases

MaxQBP08466.
PaxDbP08466.
PeptideAtlasP08466.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL208C; YJL208C; YJL208C.
GeneID853222.
KEGGsce:YJL208C.

Organism-specific databases

CYGDYJL208c.
SGDS000003744. NUC1.

Phylogenomic databases

eggNOGCOG1864.
GeneTreeENSGT00530000063772.
HOGENOMHOG000214172.
KOK01173.
OMAPNARIPN.
OrthoDBEOG7034SQ.

Enzyme and pathway databases

BioCycYEAST:G3O-31636-MONOMER.

Gene expression databases

GenevestigatorP08466.

Family and domain databases

Gene3D3.40.570.10. 1 hit.
InterProIPR018524. DNA/RNA_endonuclease_AS.
IPR001604. DNA/RNA_non-sp_Endonuclease.
IPR020821. Extracellular_endonuc_su_A.
[Graphical view]
PfamPF01223. Endonuclease_NS. 1 hit.
[Graphical view]
SMARTSM00892. Endonuclease_NS. 1 hit.
SM00477. NUC. 1 hit.
[Graphical view]
PROSITEPS01070. NUCLEASE_NON_SPEC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973422.
PROP08466.

Entry information

Entry nameNUC1_YEAST
AccessionPrimary (citable) accession number: P08466
Secondary accession number(s): D6VVY6
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: May 14, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families